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Protein

Insulin receptor-related protein

Gene

Insrr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1013ATPPROSITE-ProRule annotation1
Active sitei1115Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi985 – 993ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin receptor-related protein (EC:2.7.10.1)
Short name:
IRR
Alternative name(s):
IR-related receptor
Gene namesi
Name:Insrr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi67399. Insrr.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini747 – 921ExtracellularSequence analysisAdd BLAST175
Transmembranei922 – 942HelicalSequence analysisAdd BLAST21
Topological domaini943 – 1300CytoplasmicSequence analysisAdd BLAST358

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001670724 – 1300Insulin receptor-related proteinAdd BLAST1277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi214 ↔ 222By similarity
Disulfide bondi216 ↔ 228By similarity
Disulfide bondi229 ↔ 237By similarity
Disulfide bondi233 ↔ 246By similarity
Disulfide bondi249 ↔ 258By similarity
Disulfide bondi262 ↔ 274By similarity
Disulfide bondi280 ↔ 300By similarity
Disulfide bondi304 ↔ 317By similarity
Glycosylationi311N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi320 ↔ 324By similarity
Glycosylationi411N-linked (GlcNAc...)Sequence analysis1
Glycosylationi492N-linked (GlcNAc...)Sequence analysis1
Glycosylationi528N-linked (GlcNAc...)Sequence analysis1
Glycosylationi616N-linked (GlcNAc...)Sequence analysis1
Glycosylationi634N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi657 ↔ 864Interchain (between alpha and beta chains)Sequence analysis
Glycosylationi756N-linked (GlcNAc...)Sequence analysis1
Glycosylationi885N-linked (GlcNAc...)Sequence analysis1
Glycosylationi898N-linked (GlcNAc...)Sequence analysis1
Modified residuei1145Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1146Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated on tyrosine residues between pH 7.9 and pH 10.5.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ64716.
PRIDEiQ64716.

PTM databases

iPTMnetiQ64716.
PhosphoSitePlusiQ64716.

Expressioni

Tissue specificityi

Expressed preferentially in the kidney. Also found in stomach and thymus but not in skeletal muscle, brain, intestine, and uterus.2 Publications

Gene expression databases

BgeeiENSRNOG00000013344.
GenevisibleiQ64716. RN.

Interactioni

Subunit structurei

Probable tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018638.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini483 – 603Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST121
Domaini607 – 707Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST101
Domaini818 – 913Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST96
Domaini979 – 1254Protein kinasePROSITE-ProRule annotationAdd BLAST276

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi204 – 262Cys-richAdd BLAST59

Domaini

The extracellular domain is required for sensing alterations in external pH.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.PROSITE-ProRule annotation
Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1095. Eukaryota.
KOG4258. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118818.
HOVERGENiHBG060167.
InParanoidiQ64716.
KOiK05086.
OMAiFARTMPH.
OrthoDBiEOG091G00GE.
TreeFamiTF351636.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 4 hits.
3.80.20.20. 2 hits.
InterProiIPR003961. FN3_dom.
IPR006211. Furin-like_Cys-rich_dom.
IPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_.
IPR013783. Ig-like_fold.
IPR028792. INSRR.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR000494. Rcpt_L-dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016246. Tyr_kinase_insulin-like_rcpt.
IPR002011. Tyr_kinase_rcpt_2_CS.
[Graphical view]
PANTHERiPTHR24416:SF338. PTHR24416:SF338. 3 hits.
PfamiPF00757. Furin-like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF01030. Recep_L_domain. 2 hits.
[Graphical view]
PIRSFiPIRSF000620. Insulin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00060. FN3. 3 hits.
SM00261. FU. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 4 hits.
SSF52058. SSF52058. 3 hits.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS50853. FN3. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00239. RECEPTOR_TYR_KIN_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64716-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPALWPWG VYLLMSLLSL GSCLDTLEVC PSLDIRSEVT ELRRLENCSV
60 70 80 90 100
VEGHLQILLM FAATGEDFRG LSFPRLTQVT DYLLLFRVYG LESLRDLFPN
110 120 130 140 150
LAVIRGARLF LGYALIIFEM PHLRDIGLPS LGAVLRGAVR VEKNQELCHL
160 170 180 190 200
STIDWGLLQP APGANHIVGN KLGEECADVC PGVLGAAGEP CVRTTFGGHT
210 220 230 240 250
DYRCWTSSHC QRVCPCPRGL ACTVGGECCH SECLGGCSQP EDPRACVACR
260 270 280 290 300
HLYFQGVCLP TCPPGTYQYE SWRCVTAELC GHLREVPGHA TAFGIYEGSC
310 320 330 340 350
LAQCPPGFTR NGSSIFCHKC EGLCPKECKV GTKTIDSVQA TQDLVGCTHV
360 370 380 390 400
EGSLILNLRQ GYNLEPELQR NLGLVETITG FLKIKHSFAL VTLGFFKNLK
410 420 430 440 450
LIRGDSMVDG NYTLYVLDNQ NLQQLGSWVA AGLTIPVGKI YFAFNPRLCL
460 470 480 490 500
EHIYQLEEVT GTRGRQSKAE INPRTNGDRA ACQTRTLRFV FNLTEEDRIL
510 520 530 540 550
LRWERYEPLE ARDLLSFIVY YKESPFQNAT EHVGPDACGT QSWNLLDVEL
560 570 580 590 600
PLSRTQEPGV TLAPLKPWTQ YAVFVRAITL TTAEDSPHQG AQSPIVYLRT
610 620 630 640 650
LPAAPTVPQD VISTSNSSSH LLVRWKPPVQ RNGNITYYLV LWQRLAEDGD
660 670 680 690 700
LYINDYCHRG LRLPTSSHDT RFDREDPALE AEPEQGCCPC QHSPPGQALP
710 720 730 740 750
ALEAQEVTFQ KKFENFLHHA ITIPKAPWKV TSINKNPQRD SERHRRETGL
760 770 780 790 800
LRLGKNNSDF EIHEDKVPRE RAVLSGLRHF TEYRIDIHAC NHAAHTVGCS
810 820 830 840 850
AATFVFARTM PHREADGIPG KVVWKAVGKS SVILHWLEPP DPNGLILKYE
860 870 880 890 900
IKYRRLGEEA TVLCVSRLRY AKVGGVHLAL LPPGNYSAKV RATSLAGNGS
910 920 930 940 950
WTDGVAFYIT GPEEEDTGGL RILLTVTPVG FMLLVMLAAL GFFYSKKRNS
960 970 980 990 1000
TLYTSVNPEY FSASHMYVPD EWEVPREQIA IIRELGQGSF GMVYEGLARG
1010 1020 1030 1040 1050
LEAGEESTPV ALKTVNELAS ARERVEFLKE ASVMKAFKCH HVVRLLGVVS
1060 1070 1080 1090 1100
QGQPTLVIME LMTRGDLKSH LRSLRPEAEN NPGLPQPALS DMIQMAGEIA
1110 1120 1130 1140 1150
DGMAYLAAKK FVHRDLAARN CMVSQDFTVK IGDFGMTRDV YETDYYRKGG
1160 1170 1180 1190 1200
KGLLPVRWMA PESLKDGIFT THSDVWSFGV VLWEIVTLAE QPYQGLSNEQ
1210 1220 1230 1240 1250
VLKFVMDGGV LEEQEDCPIQ LQELMRRCWQ HTPRLRPTFV HILDRIQDEL
1260 1270 1280 1290 1300
RPSFRLCSFY YSPECQRGQA SLLPTEAEPD SPPTLNGASD YGPPNGDPGH
Length:1,300
Mass (Da):144,846
Last modified:November 16, 2011 - v3
Checksum:i0CF24D5B96D68225
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261T → A in AAB59692 (PubMed:1603082).Curated1
Sequence conflicti425L → M in AAB59692 (PubMed:1603082).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03012734 Genomic DNA. No translation available.
M90661 mRNA. Translation: AAB59692.1.
M90660 Genomic DNA. Translation: AAA41452.1.
D12678 mRNA. Translation: BAA20982.1.
PIRiPC1130.
RefSeqiNP_071548.2. NM_022212.2.
UniGeneiRn.44446.

Genome annotation databases

EnsembliENSRNOT00000080693; ENSRNOP00000073224; ENSRNOG00000057702.
GeneIDi60663.
KEGGirno:60663.
UCSCiRGD:67399. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03012734 Genomic DNA. No translation available.
M90661 mRNA. Translation: AAB59692.1.
M90660 Genomic DNA. Translation: AAA41452.1.
D12678 mRNA. Translation: BAA20982.1.
PIRiPC1130.
RefSeqiNP_071548.2. NM_022212.2.
UniGeneiRn.44446.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018638.

PTM databases

iPTMnetiQ64716.
PhosphoSitePlusiQ64716.

Proteomic databases

PaxDbiQ64716.
PRIDEiQ64716.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000080693; ENSRNOP00000073224; ENSRNOG00000057702.
GeneIDi60663.
KEGGirno:60663.
UCSCiRGD:67399. rat.

Organism-specific databases

CTDi3645.
RGDi67399. Insrr.

Phylogenomic databases

eggNOGiKOG1095. Eukaryota.
KOG4258. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118818.
HOVERGENiHBG060167.
InParanoidiQ64716.
KOiK05086.
OMAiFARTMPH.
OrthoDBiEOG091G00GE.
TreeFamiTF351636.

Miscellaneous databases

PROiQ64716.

Gene expression databases

BgeeiENSRNOG00000013344.
GenevisibleiQ64716. RN.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 4 hits.
3.80.20.20. 2 hits.
InterProiIPR003961. FN3_dom.
IPR006211. Furin-like_Cys-rich_dom.
IPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_.
IPR013783. Ig-like_fold.
IPR028792. INSRR.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR000494. Rcpt_L-dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016246. Tyr_kinase_insulin-like_rcpt.
IPR002011. Tyr_kinase_rcpt_2_CS.
[Graphical view]
PANTHERiPTHR24416:SF338. PTHR24416:SF338. 3 hits.
PfamiPF00757. Furin-like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF01030. Recep_L_domain. 2 hits.
[Graphical view]
PIRSFiPIRSF000620. Insulin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00060. FN3. 3 hits.
SM00261. FU. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 4 hits.
SSF52058. SSF52058. 3 hits.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS50853. FN3. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00239. RECEPTOR_TYR_KIN_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiINSRR_RAT
AccessioniPrimary (citable) accession number: Q64716
Secondary accession number(s): Q63146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 16, 2011
Last modified: November 30, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.