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Protein

Upstream stimulatory factor 2

Gene

Usf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functioni

GO - Biological processi

  • lactation Source: MGI
  • lipid homeostasis Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Upstream stimulatory factor 2
Alternative name(s):
Major late transcription factor 2
Upstream transcription factor 2
Gene namesi
Name:Usf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:99961. Usf2.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Upstream stimulatory factor 2PRO_0000127501Add
BLAST

Proteomic databases

MaxQBiQ64705.
PaxDbiQ64705.
PRIDEiQ64705.

PTM databases

PhosphoSiteiQ64705.

Expressioni

Gene expression databases

BgeeiQ64705.
CleanExiMM_USF2.
ExpressionAtlasiQ64705. baseline and differential.
GenevisibleiQ64705. MM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with MAF.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Stat1P422253EBI-647583,EBI-647118

Protein-protein interaction databases

IntActiQ64705. 2 interactions.
STRINGi10090.ENSMUSP00000132256.

Structurei

3D structure databases

ProteinModelPortaliQ64705.
SMRiQ64705. Positions 233-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini235 – 29056bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni307 – 32822Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 207Poly-Ala

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG272240.
GeneTreeiENSGT00390000004498.
HOGENOMiHOG000294174.
HOVERGENiHBG004346.
InParanoidiQ64705.
KOiK09106.
OMAiIPDCNTD.
OrthoDBiEOG7GXPBW.
PhylomeDBiQ64705.
TreeFamiTF323338.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform USF2A (identifier: Q64705-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA
60 70 80 90 100
IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS
110 120 130 140 150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF
160 170 180 190 200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM
210 220 230 240 250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK
260 270 280 290 300
INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
310 320 330 340
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ
Length:346
Mass (Da):36,954
Last modified:November 1, 1996 - v1
Checksum:iE9D216BC25F9447B
GO
Isoform USF2B (identifier: Q64705-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-143: Missing.

Show »
Length:279
Mass (Da):30,595
Checksum:iD9474CE884D8A60B
GO

Sequence cautioni

The sequence AAA20493.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH19729.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti240 – 2434Missing in CAA54697 (PubMed:7774954).Curated
Sequence conflicti326 – 3261A → S in CAA54697 (PubMed:7774954).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei77 – 14367Missing in isoform USF2B. 1 PublicationVSP_002166Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12283, U12282 Genomic DNA. Translation: AAB60674.1.
U01662 mRNA. Translation: AAA20492.1.
U01663 mRNA. Translation: AAA20493.1. Different initiation.
X77602, X77605 Genomic DNA. Translation: CAA54697.1.
BC019729 mRNA. Translation: AAH19729.1. Different initiation.
BC082995 mRNA. Translation: AAH82995.1.
AK146871 mRNA. Translation: BAE27494.1.
CCDSiCCDS21118.1. [Q64705-1]
PIRiA55111.
RefSeqiNP_035810.1. NM_011680.2. [Q64705-1]
XP_006539796.1. XM_006539733.2. [Q64705-2]
XP_006539797.1. XM_006539734.2.
XP_006539798.1. XM_006539735.2.
UniGeneiMm.322453.

Genome annotation databases

EnsembliENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239. [Q64705-1]
ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239. [Q64705-2]
GeneIDi22282.
KEGGimmu:22282.
UCSCiuc009ghg.2. mouse. [Q64705-2]
uc009ghh.2. mouse. [Q64705-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12283, U12282 Genomic DNA. Translation: AAB60674.1.
U01662 mRNA. Translation: AAA20492.1.
U01663 mRNA. Translation: AAA20493.1. Different initiation.
X77602, X77605 Genomic DNA. Translation: CAA54697.1.
BC019729 mRNA. Translation: AAH19729.1. Different initiation.
BC082995 mRNA. Translation: AAH82995.1.
AK146871 mRNA. Translation: BAE27494.1.
CCDSiCCDS21118.1. [Q64705-1]
PIRiA55111.
RefSeqiNP_035810.1. NM_011680.2. [Q64705-1]
XP_006539796.1. XM_006539733.2. [Q64705-2]
XP_006539797.1. XM_006539734.2.
XP_006539798.1. XM_006539735.2.
UniGeneiMm.322453.

3D structure databases

ProteinModelPortaliQ64705.
SMRiQ64705. Positions 233-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64705. 2 interactions.
STRINGi10090.ENSMUSP00000132256.

PTM databases

PhosphoSiteiQ64705.

Proteomic databases

MaxQBiQ64705.
PaxDbiQ64705.
PRIDEiQ64705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239. [Q64705-1]
ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239. [Q64705-2]
GeneIDi22282.
KEGGimmu:22282.
UCSCiuc009ghg.2. mouse. [Q64705-2]
uc009ghh.2. mouse. [Q64705-1]

Organism-specific databases

CTDi7392.
MGIiMGI:99961. Usf2.

Phylogenomic databases

eggNOGiNOG272240.
GeneTreeiENSGT00390000004498.
HOGENOMiHOG000294174.
HOVERGENiHBG004346.
InParanoidiQ64705.
KOiK09106.
OMAiIPDCNTD.
OrthoDBiEOG7GXPBW.
PhylomeDBiQ64705.
TreeFamiTF323338.

Miscellaneous databases

NextBioi302407.
PROiQ64705.
SOURCEiSearch...

Gene expression databases

BgeeiQ64705.
CleanExiMM_USF2.
ExpressionAtlasiQ64705. baseline and differential.
GenevisibleiQ64705. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Archaic structure of the gene encoding transcription factor USF."
    Lin Q., Luo X., Sawadogo M.
    J. Biol. Chem. 269:23894-23903(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Kidney and Spleen.
  2. "Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells."
    Sirito M., Lin Q., Maity T., Sawadogo M.
    Nucleic Acids Res. 22:427-433(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS USF2A AND USF2B).
  3. "Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse."
    Henrion A.A., Martinez A., Mattei M.-G., Kahn A., Raymondjean M.
    Genomics 25:36-43(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
    Strain: FVB/N and FVB/N-3.
    Tissue: Mammary tumor.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-346 (ISOFORM USF2A).
    Strain: C57BL/6J.
    Tissue: Amnion.
  6. "USF2/FIP associates with the b-Zip transcription factor, c-Maf, via its bHLH domain and inhibits c-Maf DNA binding activity."
    Kurschner C., Morgan J.I.
    Biochem. Biophys. Res. Commun. 231:333-339(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAF.

Entry informationi

Entry nameiUSF2_MOUSE
AccessioniPrimary (citable) accession number: Q64705
Secondary accession number(s): Q3UIL2, Q8VE59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.