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Q64705

- USF2_MOUSE

UniProt

Q64705 - USF2_MOUSE

Protein

Upstream stimulatory factor 2

Gene

Usf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. double-stranded DNA binding Source: Ensembl
    3. protein binding Source: IntAct
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
    5. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: Ensembl

    GO - Biological processi

    1. lactation Source: MGI
    2. lipid homeostasis Source: BHF-UCL
    3. positive regulation of transcription, DNA-templated Source: MGI
    4. positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
    5. regulation of transcription from RNA polymerase II promoter Source: MGI

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Upstream stimulatory factor 2
    Alternative name(s):
    Major late transcription factor 2
    Upstream transcription factor 2
    Gene namesi
    Name:Usf2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:99961. Usf2.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 346346Upstream stimulatory factor 2PRO_0000127501Add
    BLAST

    Proteomic databases

    MaxQBiQ64705.
    PaxDbiQ64705.
    PRIDEiQ64705.

    PTM databases

    PhosphoSiteiQ64705.

    Expressioni

    Gene expression databases

    ArrayExpressiQ64705.
    BgeeiQ64705.
    CleanExiMM_USF2.
    GenevestigatoriQ64705.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with MAF.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Stat1P422253EBI-647583,EBI-647118

    Protein-protein interaction databases

    IntActiQ64705. 2 interactions.
    STRINGi10090.ENSMUSP00000057569.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64705.
    SMRiQ64705. Positions 233-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini235 – 29056bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni307 – 32822Leucine-zipperAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi14 – 207Poly-Ala

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG272240.
    GeneTreeiENSGT00390000004498.
    HOGENOMiHOG000294174.
    HOVERGENiHBG004346.
    InParanoidiQ64705.
    KOiK09106.
    OMAiIPDCNTD.
    OrthoDBiEOG7GXPBW.
    PhylomeDBiQ64705.
    TreeFamiTF323338.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform USF2A (identifier: Q64705-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA    50
    IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS 100
    VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF 150
    SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM 200
    TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK 250
    INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET 300
    FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ 346
    Length:346
    Mass (Da):36,954
    Last modified:November 1, 1996 - v1
    Checksum:iE9D216BC25F9447B
    GO
    Isoform USF2B (identifier: Q64705-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         77-143: Missing.

    Show »
    Length:279
    Mass (Da):30,595
    Checksum:iD9474CE884D8A60B
    GO

    Sequence cautioni

    The sequence AAA20493.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH19729.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti240 – 2434Missing in CAA54697. (PubMed:7774954)Curated
    Sequence conflicti326 – 3261A → S in CAA54697. (PubMed:7774954)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei77 – 14367Missing in isoform USF2B. 1 PublicationVSP_002166Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12283, U12282 Genomic DNA. Translation: AAB60674.1.
    U01662 mRNA. Translation: AAA20492.1.
    U01663 mRNA. Translation: AAA20493.1. Different initiation.
    X77602, X77605 Genomic DNA. Translation: CAA54697.1.
    BC019729 mRNA. Translation: AAH19729.1. Different initiation.
    BC082995 mRNA. Translation: AAH82995.1.
    AK146871 mRNA. Translation: BAE27494.1.
    CCDSiCCDS21118.1. [Q64705-1]
    PIRiA55111.
    RefSeqiNP_035810.1. NM_011680.2. [Q64705-1]
    XP_006539796.1. XM_006539733.1. [Q64705-2]
    XP_006539797.1. XM_006539734.1.
    XP_006539798.1. XM_006539735.1.
    UniGeneiMm.322453.

    Genome annotation databases

    EnsembliENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239. [Q64705-1]
    ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239. [Q64705-2]
    GeneIDi22282.
    KEGGimmu:22282.
    UCSCiuc009ghg.2. mouse. [Q64705-2]
    uc009ghh.2. mouse. [Q64705-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12283 , U12282 Genomic DNA. Translation: AAB60674.1 .
    U01662 mRNA. Translation: AAA20492.1 .
    U01663 mRNA. Translation: AAA20493.1 . Different initiation.
    X77602 , X77605 Genomic DNA. Translation: CAA54697.1 .
    BC019729 mRNA. Translation: AAH19729.1 . Different initiation.
    BC082995 mRNA. Translation: AAH82995.1 .
    AK146871 mRNA. Translation: BAE27494.1 .
    CCDSi CCDS21118.1. [Q64705-1 ]
    PIRi A55111.
    RefSeqi NP_035810.1. NM_011680.2. [Q64705-1 ]
    XP_006539796.1. XM_006539733.1. [Q64705-2 ]
    XP_006539797.1. XM_006539734.1.
    XP_006539798.1. XM_006539735.1.
    UniGenei Mm.322453.

    3D structure databases

    ProteinModelPortali Q64705.
    SMRi Q64705. Positions 233-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q64705. 2 interactions.
    STRINGi 10090.ENSMUSP00000057569.

    PTM databases

    PhosphoSitei Q64705.

    Proteomic databases

    MaxQBi Q64705.
    PaxDbi Q64705.
    PRIDEi Q64705.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000058860 ; ENSMUSP00000132256 ; ENSMUSG00000058239 . [Q64705-1 ]
    ENSMUST00000108119 ; ENSMUSP00000132021 ; ENSMUSG00000058239 . [Q64705-2 ]
    GeneIDi 22282.
    KEGGi mmu:22282.
    UCSCi uc009ghg.2. mouse. [Q64705-2 ]
    uc009ghh.2. mouse. [Q64705-1 ]

    Organism-specific databases

    CTDi 7392.
    MGIi MGI:99961. Usf2.

    Phylogenomic databases

    eggNOGi NOG272240.
    GeneTreei ENSGT00390000004498.
    HOGENOMi HOG000294174.
    HOVERGENi HBG004346.
    InParanoidi Q64705.
    KOi K09106.
    OMAi IPDCNTD.
    OrthoDBi EOG7GXPBW.
    PhylomeDBi Q64705.
    TreeFami TF323338.

    Miscellaneous databases

    NextBioi 302407.
    PROi Q64705.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64705.
    Bgeei Q64705.
    CleanExi MM_USF2.
    Genevestigatori Q64705.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Archaic structure of the gene encoding transcription factor USF."
      Lin Q., Luo X., Sawadogo M.
      J. Biol. Chem. 269:23894-23903(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
      Tissue: Kidney and Spleen.
    2. "Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells."
      Sirito M., Lin Q., Maity T., Sawadogo M.
      Nucleic Acids Res. 22:427-433(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS USF2A AND USF2B).
    3. "Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse."
      Henrion A.A., Martinez A., Mattei M.-G., Kahn A., Raymondjean M.
      Genomics 25:36-43(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
      Strain: FVB/N and FVB/N-3.
      Tissue: Mammary tumor.
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-346 (ISOFORM USF2A).
      Strain: C57BL/6J.
      Tissue: Amnion.
    6. "USF2/FIP associates with the b-Zip transcription factor, c-Maf, via its bHLH domain and inhibits c-Maf DNA binding activity."
      Kurschner C., Morgan J.I.
      Biochem. Biophys. Res. Commun. 231:333-339(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAF.

    Entry informationi

    Entry nameiUSF2_MOUSE
    AccessioniPrimary (citable) accession number: Q64705
    Secondary accession number(s): Q3UIL2, Q8VE59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3