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Protein

Upstream stimulatory factor 2

Gene

Usf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functioni

GO - Biological processi

  • lactation Source: MGI
  • lipid homeostasis Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  • regulation of transcription by RNA polymerase II Source: MGI
  • transcription by RNA polymerase II Source: MGI

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Upstream stimulatory factor 2
Alternative name(s):
Major late transcription factor 2
Upstream transcription factor 2
Gene namesi
Name:Usf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:99961 Usf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275011 – 346Upstream stimulatory factor 2Add BLAST346

Proteomic databases

EPDiQ64705
PaxDbiQ64705
PRIDEiQ64705

PTM databases

iPTMnetiQ64705
PhosphoSitePlusiQ64705

Expressioni

Gene expression databases

BgeeiENSMUSG00000058239
CleanExiMM_USF2
ExpressionAtlasiQ64705 baseline and differential
GenevisibleiQ64705 MM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with MAF.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Stat1P422253EBI-647583,EBI-647118

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ64705, 2 interactors
STRINGi10090.ENSMUSP00000132256

Structurei

3D structure databases

ProteinModelPortaliQ64705
SMRiQ64705
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini235 – 290bHLHPROSITE-ProRule annotationAdd BLAST56

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni307 – 328Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 20Poly-Ala7

Phylogenomic databases

eggNOGiENOG410INSC Eukaryota
ENOG4111DV1 LUCA
GeneTreeiENSGT00390000004498
HOGENOMiHOG000294174
HOVERGENiHBG004346
InParanoidiQ64705
KOiK09106
OMAiPDCNTDN
OrthoDBiEOG091G0RH5
PhylomeDBiQ64705
TreeFamiTF323338

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform USF2A (identifier: Q64705-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA
60 70 80 90 100
IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS
110 120 130 140 150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF
160 170 180 190 200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM
210 220 230 240 250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK
260 270 280 290 300
INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
310 320 330 340
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ
Length:346
Mass (Da):36,954
Last modified:November 1, 1996 - v1
Checksum:iE9D216BC25F9447B
GO
Isoform USF2B (identifier: Q64705-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-143: Missing.

Show »
Length:279
Mass (Da):30,595
Checksum:iD9474CE884D8A60B
GO

Sequence cautioni

The sequence AAA20493 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH19729 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti240 – 243Missing in CAA54697 (PubMed:7774954).Curated4
Sequence conflicti326A → S in CAA54697 (PubMed:7774954).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00216677 – 143Missing in isoform USF2B. 1 PublicationAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12283, U12282 Genomic DNA Translation: AAB60674.1
U01662 mRNA Translation: AAA20492.1
U01663 mRNA Translation: AAA20493.1 Different initiation.
X77602, X77605 Genomic DNA Translation: CAA54697.1
BC019729 mRNA Translation: AAH19729.1 Different initiation.
BC082995 mRNA Translation: AAH82995.1
AK146871 mRNA Translation: BAE27494.1
CCDSiCCDS21118.1 [Q64705-1]
PIRiA55111
RefSeqiNP_035810.1, NM_011680.2 [Q64705-1]
XP_006539796.1, XM_006539733.3 [Q64705-2]
XP_006539797.1, XM_006539734.3
XP_006539798.1, XM_006539735.3
UniGeneiMm.322453

Genome annotation databases

EnsembliENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239 [Q64705-1]
ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239 [Q64705-2]
GeneIDi22282
KEGGimmu:22282
UCSCiuc009ghg.2 mouse [Q64705-2]
uc009ghh.2 mouse [Q64705-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUSF2_MOUSE
AccessioniPrimary (citable) accession number: Q64705
Secondary accession number(s): Q3UIL2, Q8VE59
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health