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Q64705 (USF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Upstream stimulatory factor 2
Alternative name(s):
Major late transcription factor 2
Upstream transcription factor 2
Gene names
Name:Usf2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with MAF. Ref.6

Subcellular location

Nucleus.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Sequence caution

The sequence AAA20493.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH19729.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Stat1P422253EBI-647583,EBI-647118

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform USF2A (identifier: Q64705-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform USF2B (identifier: Q64705-2)

The sequence of this isoform differs from the canonical sequence as follows:
     77-143: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Upstream stimulatory factor 2
PRO_0000127501

Regions

Domain235 – 29056bHLH
Region307 – 32822Leucine-zipper
Compositional bias14 – 207Poly-Ala

Natural variations

Alternative sequence77 – 14367Missing in isoform USF2B.
VSP_002166

Experimental info

Sequence conflict240 – 2434Missing in CAA54697. Ref.3
Sequence conflict3261A → S in CAA54697. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform USF2A [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E9D216BC25F9447B

FASTA34636,954
        10         20         30         40         50         60 
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA IASVQQAAFG 

        70         80         90        100        110        120 
DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS VVSTAAFAGG QQAVTQVGVD 

       130        140        150        160        170        180 
GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA 

       190        200        210        220        230        240 
VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH 

       250        260        270        280        290        300 
NEVERRRRDK INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET 

       310        320        330        340 
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ 

« Hide

Isoform USF2B [UniParc].

Checksum: D9474CE884D8A60B
Show »

FASTA27930,595

References

« Hide 'large scale' references
[1]"Archaic structure of the gene encoding transcription factor USF."
Lin Q., Luo X., Sawadogo M.
J. Biol. Chem. 269:23894-23903(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
Tissue: Kidney and Spleen.
[2]"Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells."
Sirito M., Lin Q., Maity T., Sawadogo M.
Nucleic Acids Res. 22:427-433(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS USF2A AND USF2B).
[3]"Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse."
Henrion A.A., Martinez A., Mattei M.-G., Kahn A., Raymondjean M.
Genomics 25:36-43(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
Strain: FVB/N and FVB/N-3.
Tissue: Mammary tumor.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-346 (ISOFORM USF2A).
Strain: C57BL/6J.
Tissue: Amnion.
[6]"USF2/FIP associates with the b-Zip transcription factor, c-Maf, via its bHLH domain and inhibits c-Maf DNA binding activity."
Kurschner C., Morgan J.I.
Biochem. Biophys. Res. Commun. 231:333-339(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAF.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12283, U12282 Genomic DNA. Translation: AAB60674.1.
U01662 mRNA. Translation: AAA20492.1.
U01663 mRNA. Translation: AAA20493.1. Different initiation.
X77602, X77605 Genomic DNA. Translation: CAA54697.1.
BC019729 mRNA. Translation: AAH19729.1. Different initiation.
BC082995 mRNA. Translation: AAH82995.1.
AK146871 mRNA. Translation: BAE27494.1.
CCDSCCDS21118.1. [Q64705-1]
PIRA55111.
RefSeqNP_035810.1. NM_011680.2. [Q64705-1]
XP_006539796.1. XM_006539733.1. [Q64705-2]
XP_006539797.1. XM_006539734.1.
XP_006539798.1. XM_006539735.1.
UniGeneMm.322453.

3D structure databases

ProteinModelPortalQ64705.
SMRQ64705. Positions 233-296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ64705. 2 interactions.
STRING10090.ENSMUSP00000057569.

PTM databases

PhosphoSiteQ64705.

Proteomic databases

MaxQBQ64705.
PaxDbQ64705.
PRIDEQ64705.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239. [Q64705-1]
ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239. [Q64705-2]
GeneID22282.
KEGGmmu:22282.
UCSCuc009ghg.2. mouse. [Q64705-2]
uc009ghh.2. mouse. [Q64705-1]

Organism-specific databases

CTD7392.
MGIMGI:99961. Usf2.

Phylogenomic databases

eggNOGNOG272240.
GeneTreeENSGT00390000004498.
HOGENOMHOG000294174.
HOVERGENHBG004346.
InParanoidQ64705.
KOK09106.
OMAIPDCNTD.
OrthoDBEOG7GXPBW.
PhylomeDBQ64705.
TreeFamTF323338.

Gene expression databases

ArrayExpressQ64705.
BgeeQ64705.
CleanExMM_USF2.
GenevestigatorQ64705.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302407.
PROQ64705.
SOURCESearch...

Entry information

Entry nameUSF2_MOUSE
AccessionPrimary (citable) accession number: Q64705
Secondary accession number(s): Q3UIL2, Q8VE59
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot