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Protein

Retinoblastoma-like protein 1

Gene

Rbl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processCell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1362277. Transcription of E2F targets under negative control by DREAM complex.
R-MMU-1362300. Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1.
R-MMU-1538133. G0 and Early G1.
R-MMU-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-MMU-539107. Activation of E2F1 target genes at G1/S.
R-MMU-69231. Cyclin D associated events in G1.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoblastoma-like protein 1
Alternative name(s):
107 kDa retinoblastoma-associated protein
Short name:
p107
pRb1
Gene namesi
Name:Rbl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:103300. Rbl1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • transcription factor complex Source: MGI

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001678401 – 1063Retinoblastoma-like protein 1Add BLAST1063

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei332PhosphothreonineCombined sources1
Modified residuei369PhosphothreonineBy similarity1
Modified residuei385PhosphothreonineBy similarity1
Modified residuei640PhosphoserineBy similarity1
Modified residuei650PhosphoserineBy similarity1
Modified residuei748PhosphoserineBy similarity1
Modified residuei761PhosphoserineBy similarity1
Modified residuei959PhosphoserineCombined sources1
Modified residuei970PhosphoserineBy similarity1
Modified residuei983PhosphoserineBy similarity1
Modified residuei992PhosphothreonineBy similarity1
Modified residuei1004PhosphoserineBy similarity1
Modified residuei1036PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated. SV40 large T antigen binds only to the unphosphorylated form, whereas the adenovirus E1A binds to both forms. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-959 and Ser-970 by CDK4 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ64701.
PaxDbiQ64701.
PeptideAtlasiQ64701.
PRIDEiQ64701.

PTM databases

iPTMnetiQ64701.
PhosphoSitePlusiQ64701.

Expressioni

Tissue specificityi

Highly expressed in fetal heart and liver. Expressed at low levels in all other fetal tissues except skeletal muscle. High levels in neonatal spleen and thymus with low levels in other tissues. In adult, highly expressed in testis. Barely detectable in other tissues.

Developmental stagei

Highly expressed in fetal tissues. Expression markedly decreased in adult.

Gene expression databases

BgeeiENSMUSG00000027641.
CleanExiMM_RBL1.
GenevisibleiQ64701. MM.

Interactioni

Subunit structurei

Interacts with AATF. Interacts with KDM5A. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (By similarity). Interacts with KMT5B, KMT5C and USP4.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CenpfQ155P73EBI-1213109,EBI-2211248

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202819. 6 interactors.
IntActiQ64701. 3 interactors.
STRINGi10090.ENSMUSP00000029170.

Structurei

3D structure databases

ProteinModelPortaliQ64701.
SMRiQ64701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni383 – 944Pocket; binds T and E1AAdd BLAST562
Regioni383 – 584Domain AAdd BLAST202
Regioni585 – 779SpacerAdd BLAST195
Regioni780 – 944Domain BAdd BLAST165

Sequence similaritiesi

Belongs to the retinoblastoma protein (RB) family.Curated

Phylogenomic databases

eggNOGiKOG1010. Eukaryota.
ENOG410XQF7. LUCA.
GeneTreeiENSGT00530000063235.
HOGENOMiHOG000273892.
HOVERGENiHBG017710.
InParanoidiQ64701.
KOiK04681.
OMAiESLAWSH.
OrthoDBiEOG091G0398.
TreeFamiTF105568.

Family and domain databases

Gene3Di1.10.472.10. 3 hits.
InterProiView protein in InterPro
IPR013763. Cyclin-like.
IPR002720. RB_A.
IPR002719. RB_B.
IPR015030. RB_C.
IPR028309. RB_fam.
IPR024599. RB_N.
IPR028310. RBL1.
PANTHERiPTHR13742. PTHR13742. 1 hit.
PTHR13742:SF24. PTHR13742:SF24. 1 hit.
PfamiView protein in Pfam
PF11934. DUF3452. 1 hit.
PF01858. RB_A. 1 hit.
PF01857. RB_B. 1 hit.
PF08934. Rb_C. 1 hit.
SMARTiView protein in SMART
SM00385. CYCLIN. 1 hit.
SM01367. DUF3452. 1 hit.
SM01368. RB_A. 1 hit.
SM01369. Rb_C. 1 hit.
SUPFAMiSSF47954. SSF47954. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q64701-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFEDEPHAEG AAAVAAAREA LQALCQELNL DEGSAAEALD DFTAIRGNYS
60 70 80 90 100
LEGEVIHWLA CSLYVACRKS IIPTVGKGVM EGNCVSLTRI LRSAKLSLIQ
110 120 130 140 150
FFSKMKKWMD MSNLPQEFRE RIERLERNFE VSTVIFKKFE PIFLDIFQNP
160 170 180 190 200
YEEPPKLPRS RKQRRIPCSV KDLFNFCWTL FVYTKGNFRM IGDDLVNSYH
210 220 230 240 250
LLLCCLDLIF ANAIMCPNRR DLLNPSFKGL PSDFHAPDFK AAEEPPCIIA
260 270 280 290 300
VLCDLHDGLL VEAKGIKEHY FKPYISKLFD KKILKGECLL DLSSFTDNSK
310 320 330 340 350
AVNKEYEEYV LTVGDFDERI FLGADAEEEI GTPRKFTADT PFGKLTSQAS
360 370 380 390 400
VECNLQQHFE KKRSFAPSTP LTGRRYLQEK EAVTTPVASA TQSVSRLQSI
410 420 430 440 450
VAGLKSAPSE QLLNIFESCM RNPMGNIIKI VKGIGETFCQ HYTQSTDKQP
460 470 480 490 500
GSHIDFAVNR LKLAEILYYK ILETIMVQET RRLHGMDMSV LLEQDIFHKS
510 520 530 540 550
LMACCLEIVL FAYSSPRTFP WIIEVLDLQP FYFYKVIEVV IRSEEGLSRD
560 570 580 590 600
MVKHLNSIEE QILESLAWTN NSALWEALHA SANRVPSCEE VIFPNNFEIG
610 620 630 640 650
NGGNVQGHLP MMPMSPIIHP RVKEVRTDSG SLRQDMQPLS PISVHERYSS
660 670 680 690 700
PAAGSAKRRL FGDDPPKDTL MDKIMAEGTK LKIAPSSVTA ESLSISPGQA
710 720 730 740 750
LLTMATTTVT GTTGRKVTVP LHGIANDAGE ITLVPISMNP TQESTAESPV
760 770 780 790 800
SLTAQSLIGT SPKQTHLTKA QDAHLTGVSK PKRTGSLALF YRKVYHLASV
810 820 830 840 850
RLRDLCLKLD VSNELRRKIW TCFEFTLVHC PDLMKDRHLD QLLLCAFYIM
860 870 880 890 900
AKVTKEERTF QEIMKSYRNQ PQANSHVYRS VLLKSIPGGV VVYNGDCEMT
910 920 930 940 950
DGDIEDATKT PNCSSEPVKE ERGDLIKFYN TVYVGRVKSF ALKYDLSNQD
960 970 980 990 1000
HIMDAPPLSP FPHIKQQPGS PRRISQQHSL YVSPHKNGAG LTPRSALLYK
1010 1020 1030 1040 1050
FNGSPSKSLK DINNMIRQGE QKTKKRVIAI SGDADSPAKR LCQENDDVLL
1060
KRLQDVVSER ANH
Length:1,063
Mass (Da):119,456
Last modified:July 27, 2011 - v3
Checksum:i85BEA8EC064EC06E
GO
Isoform Short (identifier: Q64701-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     592-594: IFP → SQG
     595-1063: Missing.

Show »
Length:594
Mass (Da):67,497
Checksum:i264EAF692D791D03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26Q → P in AAB18279 (PubMed:8661722).Curated1
Sequence conflicti430I → T in AAB18279 (PubMed:8661722).Curated1
Sequence conflicti863I → R in AAB53235 (PubMed:7490090).Curated1
Sequence conflicti989A → C in AAB18279 (PubMed:8661722).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005537592 – 594IFP → SQG in isoform Short. 1 Publication3
Alternative sequenceiVSP_005538595 – 1063Missing in isoform Short. 1 PublicationAdd BLAST469

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33320 mRNA. Translation: AAB18279.1.
U27177 mRNA. Translation: AAB53235.1.
U27178 mRNA. Translation: AAB53236.1.
AK156055 mRNA. Translation: BAE33564.1.
AL669828 Genomic DNA. Translation: CAM15973.1.
CCDSiCCDS16974.1. [Q64701-1]
PIRiI49328.
RefSeqiNP_001132988.1. NM_001139516.1. [Q64701-2]
NP_035379.2. NM_011249.2. [Q64701-1]
UniGeneiMm.244671.

Genome annotation databases

EnsembliENSMUST00000029170; ENSMUSP00000029170; ENSMUSG00000027641. [Q64701-1]
GeneIDi19650.
KEGGimmu:19650.
UCSCiuc008noq.2. mouse. [Q64701-1]
uc008nos.2. mouse. [Q64701-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRBL1_MOUSE
AccessioniPrimary (citable) accession number: Q64701
Secondary accession number(s): Q3U1D4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 5, 2017
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families