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Q64701 (RBL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Retinoblastoma-like protein 1
Alternative name(s):
107 kDa retinoblastoma-associated protein
Short name=p107
pRb1
Gene names
Name:Rbl1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1063 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor. Ref.7

Subunit structure

Interacts with AATF. Interacts with KDM5A. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 By similarity. Interacts with SUV420H1, SUV420H2 and USP4. Ref.6 Ref.7

Subcellular location

Nucleus Potential.

Tissue specificity

Highly expressed in fetal heart and liver. Expressed at low levels in all other fetal tissues except skeletal muscle. High levels in neonatal spleen and thymus with low levels in other tissues. In adult, highly expressed in testis. Barely detectable in other tissues.

Developmental stage

Highly expressed in fetal tissues. Expression markedly decreased in adult.

Post-translational modification

Phosphorylated. SV40 large T antigen binds only to the unphosphorylated form, whereas the adenovirus E1A binds to both forms. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-959 and Ser-970 by CDK4 By similarity.

Sequence similarities

Belongs to the retinoblastoma protein (RB) family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CenpfQ155P73EBI-1213109,EBI-2211248

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q64701-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q64701-2)

The sequence of this isoform differs from the canonical sequence as follows:
     592-594: IFP → SQG
     595-1063: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10631063Retinoblastoma-like protein 1
PRO_0000167840

Regions

Region383 – 944562Pocket; binds T and E1A
Region383 – 584202Domain A
Region585 – 779195Spacer
Region780 – 944165Domain B

Amino acid modifications

Modified residue3321Phosphothreonine By similarity
Modified residue3691Phosphothreonine By similarity
Modified residue3851Phosphothreonine By similarity
Modified residue6401Phosphoserine By similarity
Modified residue6501Phosphoserine By similarity
Modified residue7481Phosphoserine By similarity
Modified residue7611Phosphoserine By similarity
Modified residue9591Phosphoserine By similarity
Modified residue9701Phosphoserine By similarity
Modified residue9831Phosphoserine By similarity
Modified residue9921Phosphothreonine By similarity
Modified residue10041Phosphoserine By similarity
Modified residue10361Phosphoserine Ref.8

Natural variations

Alternative sequence592 – 5943IFP → SQG in isoform Short.
VSP_005537
Alternative sequence595 – 1063469Missing in isoform Short.
VSP_005538

Experimental info

Sequence conflict261Q → P in AAB18279. Ref.1
Sequence conflict4301I → T in AAB18279. Ref.1
Sequence conflict8631I → R in AAB53235. Ref.2
Sequence conflict9891A → C in AAB18279. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 85BEA8EC064EC06E

FASTA1,063119,456
        10         20         30         40         50         60 
MFEDEPHAEG AAAVAAAREA LQALCQELNL DEGSAAEALD DFTAIRGNYS LEGEVIHWLA 

        70         80         90        100        110        120 
CSLYVACRKS IIPTVGKGVM EGNCVSLTRI LRSAKLSLIQ FFSKMKKWMD MSNLPQEFRE 

       130        140        150        160        170        180 
RIERLERNFE VSTVIFKKFE PIFLDIFQNP YEEPPKLPRS RKQRRIPCSV KDLFNFCWTL 

       190        200        210        220        230        240 
FVYTKGNFRM IGDDLVNSYH LLLCCLDLIF ANAIMCPNRR DLLNPSFKGL PSDFHAPDFK 

       250        260        270        280        290        300 
AAEEPPCIIA VLCDLHDGLL VEAKGIKEHY FKPYISKLFD KKILKGECLL DLSSFTDNSK 

       310        320        330        340        350        360 
AVNKEYEEYV LTVGDFDERI FLGADAEEEI GTPRKFTADT PFGKLTSQAS VECNLQQHFE 

       370        380        390        400        410        420 
KKRSFAPSTP LTGRRYLQEK EAVTTPVASA TQSVSRLQSI VAGLKSAPSE QLLNIFESCM 

       430        440        450        460        470        480 
RNPMGNIIKI VKGIGETFCQ HYTQSTDKQP GSHIDFAVNR LKLAEILYYK ILETIMVQET 

       490        500        510        520        530        540 
RRLHGMDMSV LLEQDIFHKS LMACCLEIVL FAYSSPRTFP WIIEVLDLQP FYFYKVIEVV 

       550        560        570        580        590        600 
IRSEEGLSRD MVKHLNSIEE QILESLAWTN NSALWEALHA SANRVPSCEE VIFPNNFEIG 

       610        620        630        640        650        660 
NGGNVQGHLP MMPMSPIIHP RVKEVRTDSG SLRQDMQPLS PISVHERYSS PAAGSAKRRL 

       670        680        690        700        710        720 
FGDDPPKDTL MDKIMAEGTK LKIAPSSVTA ESLSISPGQA LLTMATTTVT GTTGRKVTVP 

       730        740        750        760        770        780 
LHGIANDAGE ITLVPISMNP TQESTAESPV SLTAQSLIGT SPKQTHLTKA QDAHLTGVSK 

       790        800        810        820        830        840 
PKRTGSLALF YRKVYHLASV RLRDLCLKLD VSNELRRKIW TCFEFTLVHC PDLMKDRHLD 

       850        860        870        880        890        900 
QLLLCAFYIM AKVTKEERTF QEIMKSYRNQ PQANSHVYRS VLLKSIPGGV VVYNGDCEMT 

       910        920        930        940        950        960 
DGDIEDATKT PNCSSEPVKE ERGDLIKFYN TVYVGRVKSF ALKYDLSNQD HIMDAPPLSP 

       970        980        990       1000       1010       1020 
FPHIKQQPGS PRRISQQHSL YVSPHKNGAG LTPRSALLYK FNGSPSKSLK DINNMIRQGE 

      1030       1040       1050       1060 
QKTKKRVIAI SGDADSPAKR LCQENDDVLL KRLQDVVSER ANH 

« Hide

Isoform Short [UniParc].

Checksum: 264EAF692D791D03
Show »

FASTA59467,497

References

« Hide 'large scale' references
[1]"Molecular cloning, chromosomal mapping, and expression of the mouse p107 gene."
Huppi K., Siwarski D., Mock B.A., Dosik J., Hamel P.A.
Mamm. Genome 7:353-355(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Developmental expression of p107 mRNA and evidence for alternative splicing of the p107 (RBL1) gene product."
Kim K.K., Soonpaa M.H., Wang H., Field L.J.
Genomics 28:520-529(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[3]Kim K.K., Soonpaa M.H., Wang H., Field L.J.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
Tissue: Spleen.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]"Association of UNP, a ubiquitin-specific protease, with the pocket proteins pRb, p107 and p130."
Blanchette P., Gilchrist C.A., Baker R.T., Gray D.A.
Oncogene 20:5533-5537(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH USP4.
[7]"Role of the RB1 family in stabilizing histone methylation at constitutive heterochromatin."
Gonzalo S., Garcia-Cao M., Fraga M.F., Schotta G., Peters A.H.F.M., Cotter S.E., Eguia R., Dean D.C., Esteller M., Jenuwein T., Blasco M.A.
Nat. Cell Biol. 7:420-428(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SUV420H1 AND SUV420H2.
[8]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1036, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33320 mRNA. Translation: AAB18279.1.
U27177 mRNA. Translation: AAB53235.1.
U27178 mRNA. Translation: AAB53236.1.
AK156055 mRNA. Translation: BAE33564.1.
AL669828 Genomic DNA. Translation: CAM15973.1.
CCDSCCDS16974.1. [Q64701-1]
PIRI49328.
RefSeqNP_001132988.1. NM_001139516.1. [Q64701-2]
NP_035379.2. NM_011249.2. [Q64701-1]
UniGeneMm.244671.

3D structure databases

ProteinModelPortalQ64701.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202819. 6 interactions.
IntActQ64701. 3 interactions.
STRING10090.ENSMUSP00000029170.

PTM databases

PhosphoSiteQ64701.

Proteomic databases

MaxQBQ64701.
PaxDbQ64701.
PRIDEQ64701.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029170; ENSMUSP00000029170; ENSMUSG00000027641. [Q64701-1]
GeneID19650.
KEGGmmu:19650.
UCSCuc008noq.2. mouse. [Q64701-1]
uc008nos.2. mouse. [Q64701-2]

Organism-specific databases

CTD5933.
MGIMGI:103300. Rbl1.

Phylogenomic databases

eggNOGNOG296920.
GeneTreeENSGT00530000063235.
HOGENOMHOG000273892.
HOVERGENHBG017710.
InParanoidQ3U1D4.
KOK04681.
OMARRDMQPL.
OrthoDBEOG7P5T04.
TreeFamTF105568.

Gene expression databases

BgeeQ64701.
CleanExMM_RBL1.
GenevestigatorQ64701.

Family and domain databases

Gene3D1.10.472.10. 3 hits.
InterProIPR013763. Cyclin-like.
IPR002720. RB_A.
IPR002719. RB_B.
IPR015030. RB_C.
IPR028309. RB_fam.
IPR024599. RB_N.
IPR028310. RBL1.
[Graphical view]
PANTHERPTHR13742. PTHR13742. 1 hit.
PTHR13742:SF20. PTHR13742:SF20. 1 hit.
PfamPF11934. DUF3452. 1 hit.
PF01858. RB_A. 1 hit.
PF01857. RB_B. 1 hit.
PF08934. Rb_C. 1 hit.
[Graphical view]
SMARTSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMSSF47954. SSF47954. 3 hits.
ProtoNetSearch...

Other

NextBio296908.
PROQ64701.
SOURCESearch...

Entry information

Entry nameRBL1_MOUSE
AccessionPrimary (citable) accession number: Q64701
Secondary accession number(s): Q3U1D4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot