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Q64701

- RBL1_MOUSE

UniProt

Q64701 - RBL1_MOUSE

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Protein

Retinoblastoma-like protein 1

Gene

Rbl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.1 Publication

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. chromatin modification Source: UniProtKB-KW
  3. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  4. regulation of cell cycle Source: InterPro
  5. regulation of lipid kinase activity Source: Ensembl
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_199110. G0 and Early G1.
REACT_203903. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
REACT_258573. Cyclin D associated events in G1.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoblastoma-like protein 1
Alternative name(s):
107 kDa retinoblastoma-associated protein
Short name:
p107
pRb1
Gene namesi
Name:Rbl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:103300. Rbl1.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: MGI
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10631063Retinoblastoma-like protein 1PRO_0000167840Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei332 – 3321PhosphothreonineBy similarity
Modified residuei369 – 3691PhosphothreonineBy similarity
Modified residuei385 – 3851PhosphothreonineBy similarity
Modified residuei640 – 6401PhosphoserineBy similarity
Modified residuei650 – 6501PhosphoserineBy similarity
Modified residuei748 – 7481PhosphoserineBy similarity
Modified residuei761 – 7611PhosphoserineBy similarity
Modified residuei959 – 9591PhosphoserineBy similarity
Modified residuei970 – 9701PhosphoserineBy similarity
Modified residuei983 – 9831PhosphoserineBy similarity
Modified residuei992 – 9921PhosphothreonineBy similarity
Modified residuei1004 – 10041PhosphoserineBy similarity
Modified residuei1036 – 10361Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated. SV40 large T antigen binds only to the unphosphorylated form, whereas the adenovirus E1A binds to both forms. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-959 and Ser-970 by CDK4 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ64701.
PaxDbiQ64701.
PRIDEiQ64701.

PTM databases

PhosphoSiteiQ64701.

Expressioni

Tissue specificityi

Highly expressed in fetal heart and liver. Expressed at low levels in all other fetal tissues except skeletal muscle. High levels in neonatal spleen and thymus with low levels in other tissues. In adult, highly expressed in testis. Barely detectable in other tissues.

Developmental stagei

Highly expressed in fetal tissues. Expression markedly decreased in adult.

Gene expression databases

BgeeiQ64701.
CleanExiMM_RBL1.
GenevestigatoriQ64701.

Interactioni

Subunit structurei

Interacts with AATF. Interacts with KDM5A. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (By similarity). Interacts with SUV420H1, SUV420H2 and USP4.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CenpfQ155P73EBI-1213109,EBI-2211248

Protein-protein interaction databases

BioGridi202819. 6 interactions.
IntActiQ64701. 3 interactions.
STRINGi10090.ENSMUSP00000029170.

Structurei

3D structure databases

ProteinModelPortaliQ64701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni383 – 944562Pocket; binds T and E1AAdd
BLAST
Regioni383 – 584202Domain AAdd
BLAST
Regioni585 – 779195SpacerAdd
BLAST
Regioni780 – 944165Domain BAdd
BLAST

Sequence similaritiesi

Belongs to the retinoblastoma protein (RB) family.Curated

Phylogenomic databases

eggNOGiNOG296920.
GeneTreeiENSGT00530000063235.
HOGENOMiHOG000273892.
HOVERGENiHBG017710.
InParanoidiQ64701.
KOiK04681.
OMAiRRDMQPL.
OrthoDBiEOG7P5T04.
TreeFamiTF105568.

Family and domain databases

Gene3Di1.10.472.10. 3 hits.
InterProiIPR013763. Cyclin-like.
IPR002720. RB_A.
IPR002719. RB_B.
IPR015030. RB_C.
IPR028309. RB_fam.
IPR024599. RB_N.
IPR028310. RBL1.
[Graphical view]
PANTHERiPTHR13742. PTHR13742. 1 hit.
PTHR13742:SF20. PTHR13742:SF20. 1 hit.
PfamiPF11934. DUF3452. 1 hit.
PF01858. RB_A. 1 hit.
PF01857. RB_B. 1 hit.
PF08934. Rb_C. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q64701-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFEDEPHAEG AAAVAAAREA LQALCQELNL DEGSAAEALD DFTAIRGNYS
60 70 80 90 100
LEGEVIHWLA CSLYVACRKS IIPTVGKGVM EGNCVSLTRI LRSAKLSLIQ
110 120 130 140 150
FFSKMKKWMD MSNLPQEFRE RIERLERNFE VSTVIFKKFE PIFLDIFQNP
160 170 180 190 200
YEEPPKLPRS RKQRRIPCSV KDLFNFCWTL FVYTKGNFRM IGDDLVNSYH
210 220 230 240 250
LLLCCLDLIF ANAIMCPNRR DLLNPSFKGL PSDFHAPDFK AAEEPPCIIA
260 270 280 290 300
VLCDLHDGLL VEAKGIKEHY FKPYISKLFD KKILKGECLL DLSSFTDNSK
310 320 330 340 350
AVNKEYEEYV LTVGDFDERI FLGADAEEEI GTPRKFTADT PFGKLTSQAS
360 370 380 390 400
VECNLQQHFE KKRSFAPSTP LTGRRYLQEK EAVTTPVASA TQSVSRLQSI
410 420 430 440 450
VAGLKSAPSE QLLNIFESCM RNPMGNIIKI VKGIGETFCQ HYTQSTDKQP
460 470 480 490 500
GSHIDFAVNR LKLAEILYYK ILETIMVQET RRLHGMDMSV LLEQDIFHKS
510 520 530 540 550
LMACCLEIVL FAYSSPRTFP WIIEVLDLQP FYFYKVIEVV IRSEEGLSRD
560 570 580 590 600
MVKHLNSIEE QILESLAWTN NSALWEALHA SANRVPSCEE VIFPNNFEIG
610 620 630 640 650
NGGNVQGHLP MMPMSPIIHP RVKEVRTDSG SLRQDMQPLS PISVHERYSS
660 670 680 690 700
PAAGSAKRRL FGDDPPKDTL MDKIMAEGTK LKIAPSSVTA ESLSISPGQA
710 720 730 740 750
LLTMATTTVT GTTGRKVTVP LHGIANDAGE ITLVPISMNP TQESTAESPV
760 770 780 790 800
SLTAQSLIGT SPKQTHLTKA QDAHLTGVSK PKRTGSLALF YRKVYHLASV
810 820 830 840 850
RLRDLCLKLD VSNELRRKIW TCFEFTLVHC PDLMKDRHLD QLLLCAFYIM
860 870 880 890 900
AKVTKEERTF QEIMKSYRNQ PQANSHVYRS VLLKSIPGGV VVYNGDCEMT
910 920 930 940 950
DGDIEDATKT PNCSSEPVKE ERGDLIKFYN TVYVGRVKSF ALKYDLSNQD
960 970 980 990 1000
HIMDAPPLSP FPHIKQQPGS PRRISQQHSL YVSPHKNGAG LTPRSALLYK
1010 1020 1030 1040 1050
FNGSPSKSLK DINNMIRQGE QKTKKRVIAI SGDADSPAKR LCQENDDVLL
1060
KRLQDVVSER ANH
Length:1,063
Mass (Da):119,456
Last modified:July 27, 2011 - v3
Checksum:i85BEA8EC064EC06E
GO
Isoform Short (identifier: Q64701-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     592-594: IFP → SQG
     595-1063: Missing.

Show »
Length:594
Mass (Da):67,497
Checksum:i264EAF692D791D03
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261Q → P in AAB18279. (PubMed:8661722)Curated
Sequence conflicti430 – 4301I → T in AAB18279. (PubMed:8661722)Curated
Sequence conflicti863 – 8631I → R in AAB53235. (PubMed:7490090)Curated
Sequence conflicti989 – 9891A → C in AAB18279. (PubMed:8661722)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei592 – 5943IFP → SQG in isoform Short. 1 PublicationVSP_005537
Alternative sequencei595 – 1063469Missing in isoform Short. 1 PublicationVSP_005538Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33320 mRNA. Translation: AAB18279.1.
U27177 mRNA. Translation: AAB53235.1.
U27178 mRNA. Translation: AAB53236.1.
AK156055 mRNA. Translation: BAE33564.1.
AL669828 Genomic DNA. Translation: CAM15973.1.
CCDSiCCDS16974.1. [Q64701-1]
PIRiI49328.
RefSeqiNP_001132988.1. NM_001139516.1. [Q64701-2]
NP_035379.2. NM_011249.2. [Q64701-1]
UniGeneiMm.244671.

Genome annotation databases

EnsembliENSMUST00000029170; ENSMUSP00000029170; ENSMUSG00000027641. [Q64701-1]
GeneIDi19650.
KEGGimmu:19650.
UCSCiuc008noq.2. mouse. [Q64701-1]
uc008nos.2. mouse. [Q64701-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33320 mRNA. Translation: AAB18279.1 .
U27177 mRNA. Translation: AAB53235.1 .
U27178 mRNA. Translation: AAB53236.1 .
AK156055 mRNA. Translation: BAE33564.1 .
AL669828 Genomic DNA. Translation: CAM15973.1 .
CCDSi CCDS16974.1. [Q64701-1 ]
PIRi I49328.
RefSeqi NP_001132988.1. NM_001139516.1. [Q64701-2 ]
NP_035379.2. NM_011249.2. [Q64701-1 ]
UniGenei Mm.244671.

3D structure databases

ProteinModelPortali Q64701.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202819. 6 interactions.
IntActi Q64701. 3 interactions.
STRINGi 10090.ENSMUSP00000029170.

PTM databases

PhosphoSitei Q64701.

Proteomic databases

MaxQBi Q64701.
PaxDbi Q64701.
PRIDEi Q64701.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029170 ; ENSMUSP00000029170 ; ENSMUSG00000027641 . [Q64701-1 ]
GeneIDi 19650.
KEGGi mmu:19650.
UCSCi uc008noq.2. mouse. [Q64701-1 ]
uc008nos.2. mouse. [Q64701-2 ]

Organism-specific databases

CTDi 5933.
MGIi MGI:103300. Rbl1.

Phylogenomic databases

eggNOGi NOG296920.
GeneTreei ENSGT00530000063235.
HOGENOMi HOG000273892.
HOVERGENi HBG017710.
InParanoidi Q64701.
KOi K04681.
OMAi RRDMQPL.
OrthoDBi EOG7P5T04.
TreeFami TF105568.

Enzyme and pathway databases

Reactomei REACT_199110. G0 and Early G1.
REACT_203903. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
REACT_258573. Cyclin D associated events in G1.

Miscellaneous databases

NextBioi 296908.
PROi Q64701.
SOURCEi Search...

Gene expression databases

Bgeei Q64701.
CleanExi MM_RBL1.
Genevestigatori Q64701.

Family and domain databases

Gene3Di 1.10.472.10. 3 hits.
InterProi IPR013763. Cyclin-like.
IPR002720. RB_A.
IPR002719. RB_B.
IPR015030. RB_C.
IPR028309. RB_fam.
IPR024599. RB_N.
IPR028310. RBL1.
[Graphical view ]
PANTHERi PTHR13742. PTHR13742. 1 hit.
PTHR13742:SF20. PTHR13742:SF20. 1 hit.
Pfami PF11934. DUF3452. 1 hit.
PF01858. RB_A. 1 hit.
PF01857. RB_B. 1 hit.
PF08934. Rb_C. 1 hit.
[Graphical view ]
SMARTi SM00385. CYCLIN. 1 hit.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, chromosomal mapping, and expression of the mouse p107 gene."
    Huppi K., Siwarski D., Mock B.A., Dosik J., Hamel P.A.
    Mamm. Genome 7:353-355(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Developmental expression of p107 mRNA and evidence for alternative splicing of the p107 (RBL1) gene product."
    Kim K.K., Soonpaa M.H., Wang H., Field L.J.
    Genomics 28:520-529(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
  3. Kim K.K., Soonpaa M.H., Wang H., Field L.J.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Spleen.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "Association of UNP, a ubiquitin-specific protease, with the pocket proteins pRb, p107 and p130."
    Blanchette P., Gilchrist C.A., Baker R.T., Gray D.A.
    Oncogene 20:5533-5537(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH USP4.
  7. "Role of the RB1 family in stabilizing histone methylation at constitutive heterochromatin."
    Gonzalo S., Garcia-Cao M., Fraga M.F., Schotta G., Peters A.H.F.M., Cotter S.E., Eguia R., Dean D.C., Esteller M., Jenuwein T., Blasco M.A.
    Nat. Cell Biol. 7:420-428(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SUV420H1 AND SUV420H2.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1036, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiRBL1_MOUSE
AccessioniPrimary (citable) accession number: Q64701
Secondary accession number(s): Q3U1D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3