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Q64693 (OBF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain class 2-associating factor 1
Alternative name(s):
B-cell-specific coactivator OBF-1
BOB-1
Short name=BOB1
OCA-B
OCT-binding factor 1
Gene names
Name:Pou2af1
Synonyms:Obf-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Subcellular location

Nucleus.

Tissue specificity

B-cell specific.

Post-translational modification

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation By similarity.

Sequence similarities

Belongs to the POU2AF1 family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from direct assay PubMed 11937630. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SIAH1Q8IUQ45EBI-943530,EBI-747107From a different organism.
SykP480252EBI-943530,EBI-300116

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256POU domain class 2-associating factor 1
PRO_0000058019

Experimental info

Sequence conflict501V → G in CAA91058. Ref.1
Sequence conflict97 – 982QP → HA in CAA91058. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q64693 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 392E894517EA25E9

FASTA25627,692
        10         20         30         40         50         60 
MLWQKSTAPE QAPAPPRPYQ GVRVKEPVKE LLRRKRGHTS VGAAGPPTAV VLPHQPLATY 

        70         80         90        100        110        120 
STVGPSCLDM EVSASTVTEE GTLCAGWLSQ PAPATLQPLA PWTPYTEYVS HEAVSCPYST 

       130        140        150        160        170        180 
DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTPRSTATPA VGPQLEGPEH QAPLTYFPWP 

       190        200        210        220        230        240 
QPLSTLPTSS LQYQPPAPTL SGPQFVQLPI SIPEPVLQDM DDPRRAISSL TIDKLLLEEE 

       250 
ESNTYELNHT LSVEGF 

« Hide

References

[1]"Functional characterization of the murine homolog of the B cell-specific coactivator BOB.1/OBF.1."
Pfisterer P., Zwilling S., Hess J., Wirth T.
J. Biol. Chem. 270:29870-29880(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Gene structure and characterization of the murine homologue of the B cell-specific transcriptional coactivator OBF-1."
Schubart D.B., Sauter P., Massa S., Friedl E.M., Schwarzenbach H., Matthias P.
Nucleic Acids Res. 24:1913-1920(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning and characterization of the murine B-cell specific transcriptional coactivator Bob1."
Knoepfel L., Georgiev O., Nielsen P., Schaffner W.
Biol. Chem. Hoppe-Seyler 377:139-145(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z54283 mRNA. Translation: CAA91058.1.
U43788 mRNA. Translation: AAC52618.1.
PIRS63588.
RefSeqNP_035266.1. NM_011136.2.
UniGeneMm.897.

3D structure databases

DisProtDP00008.
ProteinModelPortalQ64693.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-977N.
IntActQ64693. 3 interactions.

PTM databases

PhosphoSiteQ64693.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034554; ENSMUSP00000034554; ENSMUSG00000032053.
GeneID18985.
KEGGmmu:18985.
UCSCuc009plg.1. mouse.

Organism-specific databases

CTD5450.
MGIMGI:105086. Pou2af1.

Phylogenomic databases

eggNOGNOG80789.
GeneTreeENSGT00390000017499.
HOGENOMHOG000059584.
HOVERGENHBG007859.
InParanoidQ64693.
OMACLDMEGS.
OrthoDBEOG7JDQZ9.
PhylomeDBQ64693.
TreeFamTF332565.

Gene expression databases

ArrayExpressQ64693.
BgeeQ64693.
CleanExMM_POU2AF1.
GenevestigatorQ64693.

Family and domain databases

InterProIPR015389. PD-C2-AF1.
[Graphical view]
PfamPF09310. PD-C2-AF1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295358.
PROQ64693.
SOURCESearch...

Entry information

Entry nameOBF1_MOUSE
AccessionPrimary (citable) accession number: Q64693
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot