Q64693 (OBF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POU domain class 2-associating factor 1

Alternative name(s):
B-cell-specific coactivator OBF-1
BOB-1
Short name=BOB1
OCA-B
OCT-binding factor 1

Gene names

Name:	Pou2af1
Synonyms:	Obf-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional coactivator that specifically associates with either OCT1 or OCT2. Its boost the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centres.
Subcellular location	Nucleus.
Tissue specificity	B-cell specific.
Post-translational modification	Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation By similarity.
Sequence similarities	Belongs to the POU2AF1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Syk	P48025	2	EBI-943530,EBI-300116

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	POU domain class 2-associating factor 1		PRO_0000058019
Experimental info
	Sequence conflict	50	1	V → G in CAA91058. Ref.1
	Sequence conflict	97 – 98	2	QP → HA in CAA91058. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q64693 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 392E894517EA25E9	FASTA	256	27,692
	10 20 30 40 50 60 MLWQKSTAPE QAPAPPRPYQ GVRVKEPVKE LLRRKRGHTS VGAAGPPTAV VLPHQPLATY 70 80 90 100 110 120 STVGPSCLDM EVSASTVTEE GTLCAGWLSQ PAPATLQPLA PWTPYTEYVS HEAVSCPYST 130 140 150 160 170 180 DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTPRSTATPA VGPQLEGPEH QAPLTYFPWP 190 200 210 220 230 240 QPLSTLPTSS LQYQPPAPTL SGPQFVQLPI SIPEPVLQDM DDPRRAISSL TIDKLLLEEE 250 ESNTYELNHT LSVEGF

References

[1]	"Functional characterization of the murine homolog of the B cell-specific coactivator BOB.1/OBF.1." Pfisterer P., Zwilling S., Hess J., Wirth T. J. Biol. Chem. 270:29870-29880(1995) [PubMed: 8530384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Gene structure and characterization of the murine homologue of the B cell-specific transcriptional coactivator OBF-1." Schubart D.B., Sauter P., Massa S., Friedl E.M., Schwarzenbach H., Matthias P. Nucleic Acids Res. 24:1913-1920(1996) [PubMed: 8657574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning and characterization of the murine B-cell specific transcriptional coactivator Bob1." Knoepfel L., Georgiev O., Nielsen P., Schaffner W. Biol. Chem. Hoppe-Seyler 377:139-145(1996) [PubMed: 8868069] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z54283 mRNA. Translation: CAA91058.1. U43788 mRNA. Translation: AAC52618.1.
IPI	IPI00316974.
PIR	S63588.
RefSeq	NP_035266.1. NM_011136.2.
UniGene	Mm.897.
3D structure databases
ProteinModelPortal	Q64693.
DisProt	DP00008.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-977N.
IntAct	Q64693. 1 interaction.
STRING	Q64693.
PTM databases
PhosphoSite	Q64693.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034554; ENSMUSP00000034554; ENSMUSG00000032053.
GeneID	18985.
KEGG	mmu:18985.
NMPDR	fig|10090.3.peg.20184.
Organism-specific databases
CTD	5450.
MGI	MGI:105086. Pou2af1.
Phylogenomic databases
HOGENOM	HBG444267.
HOVERGEN	HBG007859.
InParanoid	Q64693.
OMA	YSTDMYV.
OrthoDB	EOG495ZTD.
PhylomeDB	Q64693.
Gene expression databases
ArrayExpress	Q64693.
Bgee	Q64693.
CleanEx	MM_POU2AF1.
Genevestigator	Q64693.
GermOnline	ENSMUSG00000032053. Mus musculus.
Family and domain databases
InterPro	IPR015389. PD-C2-AF1. [Graphical view]
Pfam	PF09310. PD-C2-AF1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	295358.
SOURCE	Search...

Entry information

Entry name

OBF1_MOUSE

Accession

Primary (citable) accession number: Q64693

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 21, 2011
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program