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Protein

POU domain class 2-associating factor 1

Gene

Pou2af1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

GO - Molecular functioni

  1. DNA binding Source: MGI

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain class 2-associating factor 1
Alternative name(s):
B-cell-specific coactivator OBF-1
BOB-1
Short name:
BOB1
OCA-B
OCT-binding factor 1
Gene namesi
Name:Pou2af1
Synonyms:Obf-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:105086. Pou2af1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256POU domain class 2-associating factor 1PRO_0000058019Add
BLAST

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.By similarity

Keywords - PTMi

Ubl conjugation

PTM databases

PhosphoSiteiQ64693.

Expressioni

Tissue specificityi

B-cell specific.

Gene expression databases

BgeeiQ64693.
CleanExiMM_POU2AF1.
ExpressionAtlasiQ64693. baseline and differential.
GenevestigatoriQ64693.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SIAH1Q8IUQ45EBI-943530,EBI-747107From a different organism.
SykP480252EBI-943530,EBI-300116

Protein-protein interaction databases

DIPiDIP-977N.
IntActiQ64693. 3 interactions.

Structurei

3D structure databases

DisProtiDP00008.
ProteinModelPortaliQ64693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the POU2AF1 family.Curated

Phylogenomic databases

eggNOGiNOG80789.
GeneTreeiENSGT00390000017499.
HOGENOMiHOG000059584.
HOVERGENiHBG007859.
InParanoidiQ64693.
OMAiCLDMEGS.
OrthoDBiEOG7JDQZ9.
PhylomeDBiQ64693.
TreeFamiTF332565.

Family and domain databases

InterProiIPR015389. PD-C2-AF1.
[Graphical view]
PfamiPF09310. PD-C2-AF1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWQKSTAPE QAPAPPRPYQ GVRVKEPVKE LLRRKRGHTS VGAAGPPTAV
60 70 80 90 100
VLPHQPLATY STVGPSCLDM EVSASTVTEE GTLCAGWLSQ PAPATLQPLA
110 120 130 140 150
PWTPYTEYVS HEAVSCPYST DMYVQPVCPS YTVVGPSSVL TYASPPLITN
160 170 180 190 200
VTPRSTATPA VGPQLEGPEH QAPLTYFPWP QPLSTLPTSS LQYQPPAPTL
210 220 230 240 250
SGPQFVQLPI SIPEPVLQDM DDPRRAISSL TIDKLLLEEE ESNTYELNHT

LSVEGF
Length:256
Mass (Da):27,692
Last modified:November 1, 1997 - v2
Checksum:i392E894517EA25E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501V → G in CAA91058 (PubMed:8530384).Curated
Sequence conflicti97 – 982QP → HA in CAA91058 (PubMed:8530384).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54283 mRNA. Translation: CAA91058.1.
U43788 mRNA. Translation: AAC52618.1.
CCDSiCCDS23176.1.
PIRiS63588.
RefSeqiNP_035266.1. NM_011136.2.
UniGeneiMm.897.

Genome annotation databases

EnsembliENSMUST00000034554; ENSMUSP00000034554; ENSMUSG00000032053.
GeneIDi18985.
KEGGimmu:18985.
UCSCiuc009plg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54283 mRNA. Translation: CAA91058.1.
U43788 mRNA. Translation: AAC52618.1.
CCDSiCCDS23176.1.
PIRiS63588.
RefSeqiNP_035266.1. NM_011136.2.
UniGeneiMm.897.

3D structure databases

DisProtiDP00008.
ProteinModelPortaliQ64693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-977N.
IntActiQ64693. 3 interactions.

PTM databases

PhosphoSiteiQ64693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034554; ENSMUSP00000034554; ENSMUSG00000032053.
GeneIDi18985.
KEGGimmu:18985.
UCSCiuc009plg.1. mouse.

Organism-specific databases

CTDi5450.
MGIiMGI:105086. Pou2af1.

Phylogenomic databases

eggNOGiNOG80789.
GeneTreeiENSGT00390000017499.
HOGENOMiHOG000059584.
HOVERGENiHBG007859.
InParanoidiQ64693.
OMAiCLDMEGS.
OrthoDBiEOG7JDQZ9.
PhylomeDBiQ64693.
TreeFamiTF332565.

Miscellaneous databases

NextBioi295358.
PROiQ64693.
SOURCEiSearch...

Gene expression databases

BgeeiQ64693.
CleanExiMM_POU2AF1.
ExpressionAtlasiQ64693. baseline and differential.
GenevestigatoriQ64693.

Family and domain databases

InterProiIPR015389. PD-C2-AF1.
[Graphical view]
PfamiPF09310. PD-C2-AF1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Functional characterization of the murine homolog of the B cell-specific coactivator BOB.1/OBF.1."
    Pfisterer P., Zwilling S., Hess J., Wirth T.
    J. Biol. Chem. 270:29870-29880(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Gene structure and characterization of the murine homologue of the B cell-specific transcriptional coactivator OBF-1."
    Schubart D.B., Sauter P., Massa S., Friedl E.M., Schwarzenbach H., Matthias P.
    Nucleic Acids Res. 24:1913-1920(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning and characterization of the murine B-cell specific transcriptional coactivator Bob1."
    Knoepfel L., Georgiev O., Nielsen P., Schaffner W.
    Biol. Chem. Hoppe-Seyler 377:139-145(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiOBF1_MOUSE
AccessioniPrimary (citable) accession number: Q64693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.