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Q64693

- OBF1_MOUSE

UniProt

Q64693 - OBF1_MOUSE

Protein

POU domain class 2-associating factor 1

Gene

Pou2af1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain class 2-associating factor 1
    Alternative name(s):
    B-cell-specific coactivator OBF-1
    BOB-1
    Short name:
    BOB1
    OCA-B
    OCT-binding factor 1
    Gene namesi
    Name:Pou2af1
    Synonyms:Obf-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:105086. Pou2af1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256POU domain class 2-associating factor 1PRO_0000058019Add
    BLAST

    Post-translational modificationi

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.By similarity

    Keywords - PTMi

    Ubl conjugation

    PTM databases

    PhosphoSiteiQ64693.

    Expressioni

    Tissue specificityi

    B-cell specific.

    Gene expression databases

    ArrayExpressiQ64693.
    BgeeiQ64693.
    CleanExiMM_POU2AF1.
    GenevestigatoriQ64693.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SIAH1Q8IUQ45EBI-943530,EBI-747107From a different organism.
    SykP480252EBI-943530,EBI-300116

    Protein-protein interaction databases

    DIPiDIP-977N.
    IntActiQ64693. 3 interactions.

    Structurei

    3D structure databases

    DisProtiDP00008.
    ProteinModelPortaliQ64693.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the POU2AF1 family.Curated

    Phylogenomic databases

    eggNOGiNOG80789.
    GeneTreeiENSGT00390000017499.
    HOGENOMiHOG000059584.
    HOVERGENiHBG007859.
    InParanoidiQ64693.
    OMAiCLDMEGS.
    OrthoDBiEOG7JDQZ9.
    PhylomeDBiQ64693.
    TreeFamiTF332565.

    Family and domain databases

    InterProiIPR015389. PD-C2-AF1.
    [Graphical view]
    PfamiPF09310. PD-C2-AF1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64693-1 [UniParc]FASTAAdd to Basket

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    MLWQKSTAPE QAPAPPRPYQ GVRVKEPVKE LLRRKRGHTS VGAAGPPTAV    50
    VLPHQPLATY STVGPSCLDM EVSASTVTEE GTLCAGWLSQ PAPATLQPLA 100
    PWTPYTEYVS HEAVSCPYST DMYVQPVCPS YTVVGPSSVL TYASPPLITN 150
    VTPRSTATPA VGPQLEGPEH QAPLTYFPWP QPLSTLPTSS LQYQPPAPTL 200
    SGPQFVQLPI SIPEPVLQDM DDPRRAISSL TIDKLLLEEE ESNTYELNHT 250
    LSVEGF 256
    Length:256
    Mass (Da):27,692
    Last modified:November 1, 1997 - v2
    Checksum:i392E894517EA25E9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 501V → G in CAA91058. (PubMed:8530384)Curated
    Sequence conflicti97 – 982QP → HA in CAA91058. (PubMed:8530384)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54283 mRNA. Translation: CAA91058.1.
    U43788 mRNA. Translation: AAC52618.1.
    CCDSiCCDS23176.1.
    PIRiS63588.
    RefSeqiNP_035266.1. NM_011136.2.
    UniGeneiMm.897.

    Genome annotation databases

    EnsembliENSMUST00000034554; ENSMUSP00000034554; ENSMUSG00000032053.
    GeneIDi18985.
    KEGGimmu:18985.
    UCSCiuc009plg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54283 mRNA. Translation: CAA91058.1 .
    U43788 mRNA. Translation: AAC52618.1 .
    CCDSi CCDS23176.1.
    PIRi S63588.
    RefSeqi NP_035266.1. NM_011136.2.
    UniGenei Mm.897.

    3D structure databases

    DisProti DP00008.
    ProteinModelPortali Q64693.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-977N.
    IntActi Q64693. 3 interactions.

    PTM databases

    PhosphoSitei Q64693.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034554 ; ENSMUSP00000034554 ; ENSMUSG00000032053 .
    GeneIDi 18985.
    KEGGi mmu:18985.
    UCSCi uc009plg.1. mouse.

    Organism-specific databases

    CTDi 5450.
    MGIi MGI:105086. Pou2af1.

    Phylogenomic databases

    eggNOGi NOG80789.
    GeneTreei ENSGT00390000017499.
    HOGENOMi HOG000059584.
    HOVERGENi HBG007859.
    InParanoidi Q64693.
    OMAi CLDMEGS.
    OrthoDBi EOG7JDQZ9.
    PhylomeDBi Q64693.
    TreeFami TF332565.

    Miscellaneous databases

    NextBioi 295358.
    PROi Q64693.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64693.
    Bgeei Q64693.
    CleanExi MM_POU2AF1.
    Genevestigatori Q64693.

    Family and domain databases

    InterProi IPR015389. PD-C2-AF1.
    [Graphical view ]
    Pfami PF09310. PD-C2-AF1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional characterization of the murine homolog of the B cell-specific coactivator BOB.1/OBF.1."
      Pfisterer P., Zwilling S., Hess J., Wirth T.
      J. Biol. Chem. 270:29870-29880(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "Gene structure and characterization of the murine homologue of the B cell-specific transcriptional coactivator OBF-1."
      Schubart D.B., Sauter P., Massa S., Friedl E.M., Schwarzenbach H., Matthias P.
      Nucleic Acids Res. 24:1913-1920(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Cloning and characterization of the murine B-cell specific transcriptional coactivator Bob1."
      Knoepfel L., Georgiev O., Nielsen P., Schaffner W.
      Biol. Chem. Hoppe-Seyler 377:139-145(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiOBF1_MOUSE
    AccessioniPrimary (citable) accession number: Q64693
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3