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Protein

POU domain class 2-associating factor 1

Gene

Pou2af1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain class 2-associating factor 1
Alternative name(s):
B-cell-specific coactivator OBF-1
BOB-1
Short name:
BOB1
OCA-B
OCT-binding factor 1
Gene namesi
Name:Pou2af1
Synonyms:Obf-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:105086. Pou2af1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580191 – 256POU domain class 2-associating factor 1Add BLAST256

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ64693.
PRIDEiQ64693.

PTM databases

iPTMnetiQ64693.
PhosphoSitePlusiQ64693.

Expressioni

Tissue specificityi

B-cell specific.

Gene expression databases

BgeeiENSMUSG00000032053.
CleanExiMM_POU2AF1.
ExpressionAtlasiQ64693. baseline and differential.
GenevisibleiQ64693. MM.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

DIPiDIP-977N.
IntActiQ64693. 3 interactors.
STRINGi10090.ENSMUSP00000034554.

Structurei

3D structure databases

DisProtiDP00008.
ProteinModelPortaliQ64693.
SMRiQ64693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the POU2AF1 family.Curated

Phylogenomic databases

eggNOGiENOG410IJXZ. Eukaryota.
ENOG41123CN. LUCA.
GeneTreeiENSGT00390000017499.
HOGENOMiHOG000059584.
HOVERGENiHBG007859.
InParanoidiQ64693.
OMAiCLDMEGS.
OrthoDBiEOG091G0EUL.
PhylomeDBiQ64693.
TreeFamiTF332565.

Family and domain databases

InterProiView protein in InterPro
IPR015389. PD-C2-AF1.
PfamiView protein in Pfam
PF09310. PD-C2-AF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q64693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWQKSTAPE QAPAPPRPYQ GVRVKEPVKE LLRRKRGHTS VGAAGPPTAV
60 70 80 90 100
VLPHQPLATY STVGPSCLDM EVSASTVTEE GTLCAGWLSQ PAPATLQPLA
110 120 130 140 150
PWTPYTEYVS HEAVSCPYST DMYVQPVCPS YTVVGPSSVL TYASPPLITN
160 170 180 190 200
VTPRSTATPA VGPQLEGPEH QAPLTYFPWP QPLSTLPTSS LQYQPPAPTL
210 220 230 240 250
SGPQFVQLPI SIPEPVLQDM DDPRRAISSL TIDKLLLEEE ESNTYELNHT

LSVEGF
Length:256
Mass (Da):27,692
Last modified:November 1, 1997 - v2
Checksum:i392E894517EA25E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50V → G in CAA91058 (PubMed:8530384).Curated1
Sequence conflicti97 – 98QP → HA in CAA91058 (PubMed:8530384).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54283 mRNA. Translation: CAA91058.1.
U43788 mRNA. Translation: AAC52618.1.
CCDSiCCDS23176.1.
PIRiS63588.
RefSeqiNP_035266.1. NM_011136.2.
UniGeneiMm.897.

Genome annotation databases

EnsembliENSMUST00000034554; ENSMUSP00000034554; ENSMUSG00000032053.
GeneIDi18985.
KEGGimmu:18985.
UCSCiuc009plg.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiOBF1_MOUSE
AccessioniPrimary (citable) accession number: Q64693
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 7, 2017
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families