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Protein

CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase

Gene

St8sia4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.

Pathwayi

GO - Molecular functioni

  1. alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: MGI

GO - Biological processi

  1. ganglioside biosynthetic process Source: MGI
  2. N-glycan processing Source: MGI
  3. oligosaccharide metabolic process Source: MGI
  4. protein glycosylation Source: MGI
  5. sialylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8. 3474.
ReactomeiREACT_310546. Sialic acid metabolism.
REACT_319054. NCAM1 interactions.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase (EC:2.4.99.-)
Alternative name(s):
Alpha-2,8-sialyltransferase 8D
Polysialyltransferase-1
Sialyltransferase 8D
Short name:
SIAT8-D
Sialyltransferase St8Sia IV
Short name:
ST8SiaIV
Gene namesi
Name:St8sia4
Synonyms:Pst, Siat8d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:106018. St8sia4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2013Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini21 – 359339LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferasePRO_0000149294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi142 ↔ 292By similarity
Disulfide bondi156 ↔ 356By similarity
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ64692.
PRIDEiQ64692.

PTM databases

PhosphoSiteiQ64692.

Expressioni

Tissue specificityi

Strongly expressed in lung, heart and spleen and weakly in brain.

Developmental stagei

Expressed throughout development at a low level.

Gene expression databases

BgeeiQ64692.
ExpressionAtlasiQ64692. baseline and differential.
GenevestigatoriQ64692.

Structurei

3D structure databases

ProteinModelPortaliQ64692.
SMRiQ64692. Positions 131-318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG324948.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000232070.
HOVERGENiHBG056466.
InParanoidiQ64692.
KOiK06614.
OMAiSVRKRWT.
OrthoDBiEOG7VQJD1.
PhylomeDBiQ64692.
TreeFamiTF352820.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q64692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSIRKRWTI CTISLLLIFY KTKEIARTEE HQETQLIGDG ELCLSRSLVN
60 70 80 90 100
SSDKIIRKAG STIFQHSVQG WKINSSLVLE IRKNILRFLD AERDVSVVKS
110 120 130 140 150
SFKPGDVIHY VLDRRRTLNI SHNLHSLLPE VSPMKNRRFK TCAVVGNSGI
160 170 180 190 200
LLDSGCGKEI DSHNFVIRCN LAPVVEFAAD VGTKSDFITM NPSVVQRAFG
210 220 230 240 250
GFRNESDREK FVHRLSMLND SVLWIPAFMV KGGEKHVEWV NALILKNKLQ
260 270 280 290 300
VRTAYPSLRL IHAVRGYWLT NKVPIKRPST GLLMYTLATR FCDEIHLYGF
310 320 330 340 350
WPFPKDLNGK AVKYHYYDDL KYRYFSNASP HRMPLEFKTL NVLHNRGALK

LTTGKCMKQ
Length:359
Mass (Da):41,256
Last modified:November 1, 1996 - v1
Checksum:i81FE93468579D1EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86000 mRNA. Translation: CAA59992.1.
CCDSiCCDS15201.1.
PIRiJC4224.
RefSeqiNP_001153217.1. NM_001159745.1.
NP_033209.1. NM_009183.2.
UniGeneiMm.306228.

Genome annotation databases

EnsembliENSMUST00000043336; ENSMUSP00000043477; ENSMUSG00000040710.
GeneIDi20452.
KEGGimmu:20452.
UCSCiuc007cfa.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia IV

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86000 mRNA. Translation: CAA59992.1.
CCDSiCCDS15201.1.
PIRiJC4224.
RefSeqiNP_001153217.1. NM_001159745.1.
NP_033209.1. NM_009183.2.
UniGeneiMm.306228.

3D structure databases

ProteinModelPortaliQ64692.
SMRiQ64692. Positions 131-318.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteiQ64692.

Proteomic databases

PaxDbiQ64692.
PRIDEiQ64692.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043336; ENSMUSP00000043477; ENSMUSG00000040710.
GeneIDi20452.
KEGGimmu:20452.
UCSCiuc007cfa.1. mouse.

Organism-specific databases

CTDi7903.
MGIiMGI:106018. St8sia4.

Phylogenomic databases

eggNOGiNOG324948.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000232070.
HOVERGENiHBG056466.
InParanoidiQ64692.
KOiK06614.
OMAiSVRKRWT.
OrthoDBiEOG7VQJD1.
PhylomeDBiQ64692.
TreeFamiTF352820.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.8. 3474.
ReactomeiREACT_310546. Sialic acid metabolism.
REACT_319054. NCAM1 interactions.

Miscellaneous databases

NextBioi298526.
PROiQ64692.
SOURCEiSearch...

Gene expression databases

BgeeiQ64692.
ExpressionAtlasiQ64692. baseline and differential.
GenevestigatoriQ64692.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of a third type of N-glycan alpha 2,8-sialyltransferase from mouse lung."
    Yoshida Y., Kojima N., Tsuji S.
    J. Biochem. 118:658-664(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiSIA8D_MOUSE
AccessioniPrimary (citable) accession number: Q64692
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.