Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase

Gene

St8sia4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei170SubstrateBy similarity1
Active sitei331Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8. 3474.
ReactomeiR-MMU-4085001. Sialic acid metabolism.
R-MMU-419037. NCAM1 interactions.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase (EC:2.4.99.-)
Alternative name(s):
Alpha-2,8-sialyltransferase 8D
Polysialyltransferase-1
Sialyltransferase 8D
Short name:
SIAT8-D
Sialyltransferase St8Sia IV
Short name:
ST8SiaIV
Gene namesi
Name:St8sia4
Synonyms:Pst, Siat8d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:106018. St8sia4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 20Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST13
Topological domaini21 – 359LumenalSequence analysisAdd BLAST339

GO - Cellular componenti

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492941 – 359CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferaseAdd BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi142 ↔ 292By similarity
Disulfide bondi156 ↔ 356By similarity
Glycosylationi204N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ64692.
MaxQBiQ64692.
PaxDbiQ64692.
PRIDEiQ64692.

PTM databases

PhosphoSitePlusiQ64692.

Expressioni

Tissue specificityi

Strongly expressed in lung, heart and spleen and weakly in brain.

Developmental stagei

Expressed throughout development at a low level.

Gene expression databases

BgeeiENSMUSG00000040710.
ExpressionAtlasiQ64692. baseline and differential.
GenevisibleiQ64692. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043477.

Structurei

3D structure databases

ProteinModelPortaliQ64692.
SMRiQ64692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 193Substrate bindingBy similarity3
Regioni279 – 281Substrate bindingBy similarity3
Regioni314 – 315Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000232070.
HOVERGENiHBG056466.
InParanoidiQ64692.
KOiK06614.
OMAiHSVEGWK.
OrthoDBiEOG091G0AL4.
PhylomeDBiQ64692.
TreeFamiTF352820.

Family and domain databases

InterProiView protein in InterPro
IPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
PfamiView protein in Pfam
PF00777. Glyco_transf_29. 1 hit.
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q64692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSIRKRWTI CTISLLLIFY KTKEIARTEE HQETQLIGDG ELCLSRSLVN
60 70 80 90 100
SSDKIIRKAG STIFQHSVQG WKINSSLVLE IRKNILRFLD AERDVSVVKS
110 120 130 140 150
SFKPGDVIHY VLDRRRTLNI SHNLHSLLPE VSPMKNRRFK TCAVVGNSGI
160 170 180 190 200
LLDSGCGKEI DSHNFVIRCN LAPVVEFAAD VGTKSDFITM NPSVVQRAFG
210 220 230 240 250
GFRNESDREK FVHRLSMLND SVLWIPAFMV KGGEKHVEWV NALILKNKLQ
260 270 280 290 300
VRTAYPSLRL IHAVRGYWLT NKVPIKRPST GLLMYTLATR FCDEIHLYGF
310 320 330 340 350
WPFPKDLNGK AVKYHYYDDL KYRYFSNASP HRMPLEFKTL NVLHNRGALK

LTTGKCMKQ
Length:359
Mass (Da):41,256
Last modified:November 1, 1996 - v1
Checksum:i81FE93468579D1EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86000 mRNA. Translation: CAA59992.1.
CCDSiCCDS15201.1.
PIRiJC4224.
RefSeqiNP_001153217.1. NM_001159745.1.
NP_033209.1. NM_009183.2.
UniGeneiMm.306228.

Genome annotation databases

EnsembliENSMUST00000043336; ENSMUSP00000043477; ENSMUSG00000040710.
GeneIDi20452.
KEGGimmu:20452.
UCSCiuc007cfa.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSIA8D_MOUSE
AccessioniPrimary (citable) accession number: Q64692
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families