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Q64692 (SIA8D_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase

EC=2.4.99.-
Alternative name(s):
Alpha-2,8-sialyltransferase 8D
Polysialyltransferase-1
Sialyltransferase 8D
Short name=SIAT8-D
Sialyltransferase St8Sia IV
Short name=ST8SiaIV
Gene names
Name:St8sia4
Synonyms:Pst, Siat8d
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Tissue specificity

Strongly expressed in lung, heart and spleen and weakly in brain.

Developmental stage

Expressed throughout development at a low level.

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase
PRO_0000149294

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2013Helical; Signal-anchor for type II membrane protein; Potential
Topological domain21 – 359339Lumenal Potential

Amino acid modifications

Glycosylation501N-linked (GlcNAc...) Potential
Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation1191N-linked (GlcNAc...) Potential
Glycosylation2041N-linked (GlcNAc...) Potential
Glycosylation2191N-linked (GlcNAc...) Potential
Disulfide bond142 ↔ 292 By similarity
Disulfide bond156 ↔ 356 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64692 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 81FE93468579D1EE

FASTA35941,256
        10         20         30         40         50         60 
MRSIRKRWTI CTISLLLIFY KTKEIARTEE HQETQLIGDG ELCLSRSLVN SSDKIIRKAG 

        70         80         90        100        110        120 
STIFQHSVQG WKINSSLVLE IRKNILRFLD AERDVSVVKS SFKPGDVIHY VLDRRRTLNI 

       130        140        150        160        170        180 
SHNLHSLLPE VSPMKNRRFK TCAVVGNSGI LLDSGCGKEI DSHNFVIRCN LAPVVEFAAD 

       190        200        210        220        230        240 
VGTKSDFITM NPSVVQRAFG GFRNESDREK FVHRLSMLND SVLWIPAFMV KGGEKHVEWV 

       250        260        270        280        290        300 
NALILKNKLQ VRTAYPSLRL IHAVRGYWLT NKVPIKRPST GLLMYTLATR FCDEIHLYGF 

       310        320        330        340        350 
WPFPKDLNGK AVKYHYYDDL KYRYFSNASP HRMPLEFKTL NVLHNRGALK LTTGKCMKQ 

« Hide

References

[1]"Molecular cloning and characterization of a third type of N-glycan alpha 2,8-sialyltransferase from mouse lung."
Yoshida Y., Kojima N., Tsuji S.
J. Biochem. 118:658-664(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X86000 mRNA. Translation: CAA59992.1.
PIRJC4224.
RefSeqNP_001153217.1. NM_001159745.1.
NP_033209.1. NM_009183.2.
UniGeneMm.306228.

3D structure databases

ProteinModelPortalQ64692.
SMRQ64692. Positions 62-355.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteQ64692.

Proteomic databases

PaxDbQ64692.
PRIDEQ64692.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043336; ENSMUSP00000043477; ENSMUSG00000040710.
GeneID20452.
KEGGmmu:20452.
UCSCuc007cfa.1. mouse.

Organism-specific databases

CTD7903.
MGIMGI:106018. St8sia4.

Phylogenomic databases

eggNOGNOG324948.
GeneTreeENSGT00550000074407.
HOGENOMHOG000232070.
HOVERGENHBG056466.
InParanoidQ64692.
KOK06614.
OMASVRKRWT.
OrthoDBEOG7VQJD1.
PhylomeDBQ64692.
TreeFamTF352820.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ64692.
BgeeQ64692.
GenevestigatorQ64692.

Family and domain databases

InterProIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetSearch...

Other

NextBio298526.
PROQ64692.
SOURCESearch...

Entry information

Entry nameSIA8D_MOUSE
AccessionPrimary (citable) accession number: Q64692
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot