Q64692 (SIA8D_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase
EC=2.4.99.-

Alternative name(s):
Alpha-2,8-sialyltransferase 8D
Polysialyltransferase-1
Sialyltransferase 8D
Short name=SIAT8-D
Sialyltransferase St8Sia IV
Short name=ST8SiaIV

Gene names

Name:	St8sia4
Synonyms:	Pst, Siat8d

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Strongly expressed in lung, heart and spleen and weakly in brain.
Developmental stage	Expressed throughout development at a low level.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-glycan processing Inferred from electronic annotation. Source: Compara ganglioside biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase		PRO_0000149294
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 20	13	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	21 – 359	339	Lumenal Potential
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Disulfide bond	142 ↔ 292		By similarity
	Disulfide bond	156 ↔ 356		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64692 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 81FE93468579D1EE	FASTA	359	41,256
	10 20 30 40 50 60 MRSIRKRWTI CTISLLLIFY KTKEIARTEE HQETQLIGDG ELCLSRSLVN SSDKIIRKAG 70 80 90 100 110 120 STIFQHSVQG WKINSSLVLE IRKNILRFLD AERDVSVVKS SFKPGDVIHY VLDRRRTLNI 130 140 150 160 170 180 SHNLHSLLPE VSPMKNRRFK TCAVVGNSGI LLDSGCGKEI DSHNFVIRCN LAPVVEFAAD 190 200 210 220 230 240 VGTKSDFITM NPSVVQRAFG GFRNESDREK FVHRLSMLND SVLWIPAFMV KGGEKHVEWV 250 260 270 280 290 300 NALILKNKLQ VRTAYPSLRL IHAVRGYWLT NKVPIKRPST GLLMYTLATR FCDEIHLYGF 310 320 330 340 350 WPFPKDLNGK AVKYHYYDDL KYRYFSNASP HRMPLEFKTL NVLHNRGALK LTTGKCMKQ

References

[1]	"Molecular cloning and characterization of a third type of N-glycan alpha 2,8-sialyltransferase from mouse lung." Yoshida Y., Kojima N., Tsuji S. J. Biochem. 118:658-664(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

ST8Sia IV

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86000 mRNA. Translation: CAA59992.1.
IPI	IPI00136968.
PIR	JC4224.
RefSeq	NP_001153217.1. NM_001159745.1. NP_033209.1. NM_009183.2.
UniGene	Mm.306228.
3D structure databases
ProteinModelPortal	Q64692.
SMR	Q64692. Positions 131-318.
ModBase	Search...
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	Q64692.
Proteomic databases
PaxDb	Q64692.
PRIDE	Q64692.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000043336; ENSMUSP00000043477; ENSMUSG00000040710.
GeneID	20452.
KEGG	mmu:20452.
Organism-specific databases
CTD	7903.
MGI	MGI:106018. St8sia4.
Phylogenomic databases
eggNOG	NOG324948.
GeneTree	ENSGT00550000074407.
HOGENOM	HOG000232070.
HOVERGEN	HBG056466.
InParanoid	Q64692.
KO	K06614.
OMA	DSGCGKE.
OrthoDB	EOG4DFPNN.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q64692.
Bgee	Q64692.
Genevestigator	Q64692.
GermOnline	ENSMUSG00000040710. Mus musculus.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other
NextBio	298526.
SOURCE	Search...

Entry information

Entry name

SIA8D_MOUSE

Accession

Primary (citable) accession number: Q64692

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program