Reviewed, UniProtKB/Swiss-Prot Q64690 (SIA8D_CRIGR)

Names and origin

Protein names

Recommended name:
    CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase
    EC=2.4.99.-
Alternative name(s):
    Alpha-2,8-sialyltransferase 8D
    ST8Sia IV
    Polysialyltransferase-1

Gene names

Name:	ST8SIA4
Synonyms:	PST-1, PST1, SIAT8D

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	sialyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase		PRO_0000149292
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 20	13	Signal-anchor for type II membrane protein Potential
	Topological domain	21 – 359	339	Lumenal Potential
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Disulfide bond	142 ↔ 292		By similarity
	Disulfide bond	156 ↔ 356		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64690-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C31AF4C27F8A336F	FASTA	359	41,227
	10 20 30 40 50 60 MRSIRKRWTI CTISLLLIFY KTKEIARTEE HQETQLIGDG ELCLSRSLVN SSDKIIRKAG 70 80 90 100 110 120 STIFQHSVQG WRINSSLVLE IRKNILRFLD AERDVSVVKS SFKPGDVIHY VLDRRRTLNI 130 140 150 160 170 180 SHDLHSLLPE VSPMKNRRFK TCAVVGNSGI LLDSGCGKEI DSHNFVIRCN LAPVVEFAAD 190 200 210 220 230 240 VGTKSDFITM NPSVVQRAFG GFRNESDRAK FVHRLSMLND SVLWIPAFMV KGGEKHVEWV 250 260 270 280 290 300 NALILKNKLK VRTAYPSLRL IHAVRGYWLT NKVPIKRPST GLLMYTLATR FCDEIHLYGF 310 320 330 340 350 WPFPKDLNGK AVKYHYYDDL KYRYFSNASP HRMPLEFKTL NVLHNRGALK LTTGKCMKQ

References

[1]	"Molecular characterization of eukaryotic polysialyltransferase-1." Eckhardt M., Muehlenhoff M., Bethe A., Koopman J., Frosch M., Gerardy-Schahn R. Nature 373:715-718(1995) [PubMed: 7854457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46801 mRNA. Translation: CAA86822.1.
PIR	S52425.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Phylogenomic databases
HOVERGEN	Q64690.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SIA8D_CRIGR

Accession

Primary (citable) accession number: Q64690

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 14, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)