Q64687 (SIA8A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
EC=2.4.99.8

Alternative name(s):
Alpha-2,8-sialyltransferase 8A
Ganglioside GD3 synthase
Ganglioside GT3 synthase
Sialyltransferase 8A
Short name=SIAT8-A
Sialyltransferase St8Sia I
Short name=ST8SiaI

Gene names

Name:	St8sia1
Synonyms:	Siat8, Siat8a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid. Ref.1
Catalytic activity	CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R = CMP + alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R. Ref.1
Pathway	Protein modification; protein glycosylation. Lipid metabolism; sphingolipid metabolism.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Sphingolipid metabolism
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to heat Inferred from direct assay PubMed 18755693. Source: MGI positive regulation of cell proliferation Inferred from direct assay PubMed 15748692. Source: MGI protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway sphingolipid metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase		PRO_0000149283
Regions
	Topological domain	1 – 28	28	Cytoplasmic Potential
	Transmembrane	29 – 47	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	48 – 355	308	Lumenal Potential
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Glycosylation	244	1	N-linked (GlcNAc...) Potential
	Disulfide bond	137 ↔ 286		By similarity
Experimental info
	Sequence conflict	50	1	L → P in CAA59014. Ref.1
	Sequence conflict	64	1	R → S in CAA59014. Ref.1
	Sequence conflict	294	1	F → S in CAA59014. Ref.1
	Sequence conflict	318	1	S → T in CAA59014. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q64687 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 220BD56BBEE6E6C6	FASTA	355	40,324
	10 20 30 40 50 60 MSPCGRALHT SRGAMAMLAR KFPRTRLPVG ASALCVVVLC WLYIFPVYRL PNEKEIVQGV 70 80 90 100 110 120 LAQRTAWRTN QTSASLFRRQ MEDCCDPAHL FAMTKMNSPM GKSLWYDGEL LYSFTIDNST 130 140 150 160 170 180 YSLFPQATPF QLPLKKCAVV GNGGILKMSG CGRQIDEANF VMRCNLPPLS SEYTRDVGSK 190 200 210 220 230 240 TQLVTANPSI IRQRFENLLW SRKKFVDNMK IYNHSYIYMP AFSMKTGTEP SLRVYYTLKD 250 260 270 280 290 300 VGANQTVLFA NPNFLRNIGK FWKSRGIHAK RLSTGLFLVS AALGLCEEVS IYGFWPFSVN 310 320 330 340 350 MQGDPISHHY YDNVLPFSGY HAMPEEFLQL WYLHKIGALR MQLDPCEEPS PQPTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3." Kono M., Yoshida Y., Kojima N., Tsuji S. J. Biol. Chem. 271:29366-29371(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY. Tissue: Brain.
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[4]	"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach." Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R. Glycobiology 15:805-817(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

ST8Sia I

Cross-references

Sequence databases
EMBL GenBank DDBJ	X84235 mRNA. Translation: CAA59014.1. CH466572 Genomic DNA. Translation: EDL10653.1. BC024821 mRNA. Translation: AAH24821.1.
IPI	IPI00136943.
RefSeq	NP_035504.2. NM_011374.2.
UniGene	Mm.260838.
3D structure databases
ProteinModelPortal	Q64687.
SMR	Q64687. Positions 135-314.
ModBase	Search...
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	Q64687.
Proteomic databases
PaxDb	Q64687.
PRIDE	Q64687.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032421; ENSMUSP00000032421; ENSMUSG00000030283.
GeneID	20449.
KEGG	mmu:20449.
Organism-specific databases
CTD	6489.
MGI	MGI:106011. St8sia1.
Phylogenomic databases
eggNOG	NOG272094.
GeneTree	ENSGT00550000074407.
HOGENOM	HOG000090201.
HOVERGEN	HBG106106.
InParanoid	Q8K1C1.
KO	K03371.
OMA	HTSRGAM.
OrthoDB	EOG4NZTTK.
Enzyme and pathway databases
UniPathway	UPA00222. UPA00378.
Gene expression databases
ArrayExpress	Q64687.
Bgee	Q64687.
Genevestigator	Q64687.
GermOnline	ENSMUSG00000030283. Mus musculus.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other
NextBio	298514.
SOURCE	Search...

Entry information

Entry name

SIA8A_MOUSE

Accession

Primary (citable) accession number: Q64687
Secondary accession number(s): Q8K1C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program