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Q64687 (SIA8A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase

EC=2.4.99.8
Alternative name(s):
Alpha-2,8-sialyltransferase 8A
Ganglioside GD3 synthase
Ganglioside GT3 synthase
Sialyltransferase 8A
Short name=SIAT8-A
Sialyltransferase St8Sia I
Short name=ST8SiaI
Gene names
Name:St8sia1
Synonyms:Siat8, Siat8a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid. Ref.1

Catalytic activity

CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R = CMP + alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R. Ref.1

Pathway

Protein modification; protein glycosylation.

Lipid metabolism; sphingolipid metabolism.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
PRO_0000149283

Regions

Topological domain1 – 2828Cytoplasmic Potential
Transmembrane29 – 4719Helical; Signal-anchor for type II membrane protein; Potential
Topological domain48 – 355308Lumenal Potential

Amino acid modifications

Glycosylation701N-linked (GlcNAc...) Potential
Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation2131N-linked (GlcNAc...) Potential
Glycosylation2441N-linked (GlcNAc...) Potential
Disulfide bond137 ↔ 286 By similarity

Experimental info

Sequence conflict501L → P in CAA59014. Ref.1
Sequence conflict641R → S in CAA59014. Ref.1
Sequence conflict2941F → S in CAA59014. Ref.1
Sequence conflict3181S → T in CAA59014. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q64687 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 220BD56BBEE6E6C6

FASTA35540,324
        10         20         30         40         50         60 
MSPCGRALHT SRGAMAMLAR KFPRTRLPVG ASALCVVVLC WLYIFPVYRL PNEKEIVQGV 

        70         80         90        100        110        120 
LAQRTAWRTN QTSASLFRRQ MEDCCDPAHL FAMTKMNSPM GKSLWYDGEL LYSFTIDNST 

       130        140        150        160        170        180 
YSLFPQATPF QLPLKKCAVV GNGGILKMSG CGRQIDEANF VMRCNLPPLS SEYTRDVGSK 

       190        200        210        220        230        240 
TQLVTANPSI IRQRFENLLW SRKKFVDNMK IYNHSYIYMP AFSMKTGTEP SLRVYYTLKD 

       250        260        270        280        290        300 
VGANQTVLFA NPNFLRNIGK FWKSRGIHAK RLSTGLFLVS AALGLCEEVS IYGFWPFSVN 

       310        320        330        340        350 
MQGDPISHHY YDNVLPFSGY HAMPEEFLQL WYLHKIGALR MQLDPCEEPS PQPTS 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3."
Kono M., Yoshida Y., Kojima N., Tsuji S.
J. Biol. Chem. 271:29366-29371(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
Tissue: Brain.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[4]"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach."
Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R.
Glycobiology 15:805-817(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X84235 mRNA. Translation: CAA59014.1.
CH466572 Genomic DNA. Translation: EDL10653.1.
BC024821 mRNA. Translation: AAH24821.1.
CCDSCCDS20689.1.
RefSeqNP_035504.2. NM_011374.2.
UniGeneMm.260838.

3D structure databases

ProteinModelPortalQ64687.
SMRQ64687. Positions 135-314.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteQ64687.

Proteomic databases

PaxDbQ64687.
PRIDEQ64687.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032421; ENSMUSP00000032421; ENSMUSG00000030283.
GeneID20449.
KEGGmmu:20449.
UCSCuc009epv.1. mouse.

Organism-specific databases

CTD6489.
MGIMGI:106011. St8sia1.

Phylogenomic databases

eggNOGNOG272094.
GeneTreeENSGT00550000074407.
HOGENOMHOG000090201.
HOVERGENHBG106106.
InParanoidQ8K1C1.
KOK03371.
OMACGRARRH.
OrthoDBEOG7SV0V9.
TreeFamTF323961.

Enzyme and pathway databases

UniPathwayUPA00222.
UPA00378.

Gene expression databases

ArrayExpressQ64687.
BgeeQ64687.
GenevestigatorQ64687.

Family and domain databases

InterProIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetSearch...

Other

NextBio298514.
PROQ64687.
SOURCESearch...

Entry information

Entry nameSIA8A_MOUSE
AccessionPrimary (citable) accession number: Q64687
Secondary accession number(s): Q8K1C1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot