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Q64687

- SIA8A_MOUSE

UniProt

Q64687 - SIA8A_MOUSE

Protein

Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase

Gene

St8sia1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid.1 Publication

    Catalytic activityi

    CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R = CMP + alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to heat Source: MGI
    2. positive regulation of cell proliferation Source: MGI
    3. protein glycosylation Source: UniProtKB-UniPathway
    4. sphingolipid metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_202500. Sialic acid metabolism.
    UniPathwayiUPA00222.
    UPA00378.

    Protein family/group databases

    CAZyiGT29. Glycosyltransferase Family 29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (EC:2.4.99.8)
    Alternative name(s):
    Alpha-2,8-sialyltransferase 8A
    Ganglioside GD3 synthase
    Ganglioside GT3 synthase
    Sialyltransferase 8A
    Short name:
    SIAT8-A
    Sialyltransferase St8Sia I
    Short name:
    ST8SiaI
    Gene namesi
    Name:St8sia1
    Synonyms:Siat8, Siat8a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:106011. St8sia1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of Golgi membrane Source: InterPro

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 355355Alpha-N-acetylneuraminide alpha-2,8-sialyltransferasePRO_0000149283Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi70 – 701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi137 ↔ 286By similarity
    Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ64687.
    PRIDEiQ64687.

    PTM databases

    PhosphoSiteiQ64687.

    Expressioni

    Gene expression databases

    ArrayExpressiQ64687.
    BgeeiQ64687.
    GenevestigatoriQ64687.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64687.
    SMRiQ64687. Positions 135-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini48 – 355308LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 4719Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG272094.
    GeneTreeiENSGT00550000074407.
    HOGENOMiHOG000090201.
    HOVERGENiHBG106106.
    InParanoidiQ8K1C1.
    KOiK03371.
    OMAiCGRARRH.
    OrthoDBiEOG7SV0V9.
    TreeFamiTF323961.

    Family and domain databases

    InterProiIPR001675. Glyco_trans_29.
    IPR012163. Sialyl_trans.
    [Graphical view]
    PfamiPF00777. Glyco_transf_29. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q64687-1 [UniParc]FASTAAdd to Basket

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    MSPCGRALHT SRGAMAMLAR KFPRTRLPVG ASALCVVVLC WLYIFPVYRL    50
    PNEKEIVQGV LAQRTAWRTN QTSASLFRRQ MEDCCDPAHL FAMTKMNSPM 100
    GKSLWYDGEL LYSFTIDNST YSLFPQATPF QLPLKKCAVV GNGGILKMSG 150
    CGRQIDEANF VMRCNLPPLS SEYTRDVGSK TQLVTANPSI IRQRFENLLW 200
    SRKKFVDNMK IYNHSYIYMP AFSMKTGTEP SLRVYYTLKD VGANQTVLFA 250
    NPNFLRNIGK FWKSRGIHAK RLSTGLFLVS AALGLCEEVS IYGFWPFSVN 300
    MQGDPISHHY YDNVLPFSGY HAMPEEFLQL WYLHKIGALR MQLDPCEEPS 350
    PQPTS 355
    Length:355
    Mass (Da):40,324
    Last modified:July 27, 2011 - v2
    Checksum:i220BD56BBEE6E6C6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 501L → P in CAA59014. (PubMed:8910600)Curated
    Sequence conflicti64 – 641R → S in CAA59014. (PubMed:8910600)Curated
    Sequence conflicti294 – 2941F → S in CAA59014. (PubMed:8910600)Curated
    Sequence conflicti318 – 3181S → T in CAA59014. (PubMed:8910600)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84235 mRNA. Translation: CAA59014.1.
    CH466572 Genomic DNA. Translation: EDL10653.1.
    BC024821 mRNA. Translation: AAH24821.1.
    CCDSiCCDS20689.1.
    RefSeqiNP_035504.2. NM_011374.2.
    UniGeneiMm.260838.

    Genome annotation databases

    EnsembliENSMUST00000032421; ENSMUSP00000032421; ENSMUSG00000030283.
    GeneIDi20449.
    KEGGimmu:20449.
    UCSCiuc009epv.1. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    ST8Sia I

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84235 mRNA. Translation: CAA59014.1 .
    CH466572 Genomic DNA. Translation: EDL10653.1 .
    BC024821 mRNA. Translation: AAH24821.1 .
    CCDSi CCDS20689.1.
    RefSeqi NP_035504.2. NM_011374.2.
    UniGenei Mm.260838.

    3D structure databases

    ProteinModelPortali Q64687.
    SMRi Q64687. Positions 135-314.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT29. Glycosyltransferase Family 29.

    PTM databases

    PhosphoSitei Q64687.

    Proteomic databases

    PaxDbi Q64687.
    PRIDEi Q64687.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032421 ; ENSMUSP00000032421 ; ENSMUSG00000030283 .
    GeneIDi 20449.
    KEGGi mmu:20449.
    UCSCi uc009epv.1. mouse.

    Organism-specific databases

    CTDi 6489.
    MGIi MGI:106011. St8sia1.

    Phylogenomic databases

    eggNOGi NOG272094.
    GeneTreei ENSGT00550000074407.
    HOGENOMi HOG000090201.
    HOVERGENi HBG106106.
    InParanoidi Q8K1C1.
    KOi K03371.
    OMAi CGRARRH.
    OrthoDBi EOG7SV0V9.
    TreeFami TF323961.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    UPA00378 .
    Reactomei REACT_202500. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 298514.
    PROi Q64687.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64687.
    Bgeei Q64687.
    Genevestigatori Q64687.

    Family and domain databases

    InterProi IPR001675. Glyco_trans_29.
    IPR012163. Sialyl_trans.
    [Graphical view ]
    Pfami PF00777. Glyco_transf_29. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005557. Sialyl_trans. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3."
      Kono M., Yoshida Y., Kojima N., Tsuji S.
      J. Biol. Chem. 271:29366-29371(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
      Tissue: Brain.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    4. "The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach."
      Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R.
      Glycobiology 15:805-817(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiSIA8A_MOUSE
    AccessioniPrimary (citable) accession number: Q64687
    Secondary accession number(s): Q8K1C1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3