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Protein

Beta-galactoside alpha-2,6-sialyltransferase 1

Gene

St6gal1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.

Catalytic activityi

CMP-N-acetyl-beta-neuraminate + beta-D-galactosyl-R = CMP + N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosyl-R.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186Substrate; via amide nitrogenBy similarity1
Binding sitei209SubstrateBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei350Substrate; via carbonyl oxygenBy similarity1
Binding sitei351SubstrateBy similarity1
Binding sitei362SubstrateBy similarity1
Binding sitei366SubstrateBy similarity1
Binding sitei367SubstrateBy similarity1
Binding sitei373SubstrateBy similarity1

GO - Molecular functioni

  • beta-galactoside alpha-2,6-sialyltransferase activity Source: MGI
  • sialyltransferase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.1 3474
ReactomeiR-MMU-4085001 Sialic acid metabolism
R-MMU-975577 N-Glycan antennae elongation
R-MMU-977068 Termination of O-glycan biosynthesis
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 1 (EC:2.4.99.1By similarity)
Short name:
Alpha 2,6-ST 1
Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
ST6Gal I
Short name:
ST6GalI
Sialyltransferase 1
Gene namesi
Name:St6gal1
Synonyms:Siat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:108470 St6gal1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini27 – 403LumenalSequence analysisAdd BLAST377

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492501 – 403Beta-galactoside alpha-2,6-sialyltransferase 1Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi139 ↔ 4031 Publication
Glycosylationi146N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi158N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi181 ↔ 3321 Publication
Disulfide bondi350 ↔ 3611 Publication
Modified residuei366PhosphotyrosineBy similarity1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ64685
MaxQBiQ64685
PaxDbiQ64685
PeptideAtlasiQ64685
PRIDEiQ64685

PTM databases

iPTMnetiQ64685
PhosphoSitePlusiQ64685

Miscellaneous databases

PMAP-CutDBiQ8K1L1

Expressioni

Gene expression databases

BgeeiENSMUSG00000022885
CleanExiMM_ST6GAL1
ExpressionAtlasiQ64685 baseline and differential
GenevisibleiQ64685 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023601

Structurei

3D structure databases

ProteinModelPortaliQ64685
SMRiQ64685
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni319 – 321Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074444
HOGENOMiHOG000013206
HOVERGENiHBG052853
InParanoidiQ64685
KOiK00778
OMAiSFQVWNK
OrthoDBiEOG091G07C7
TreeFamiTF323961

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit

Sequencei

Sequence statusi: Complete.

Q64685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHTNLKRKF SCFVLVFLLF AIICVWKKGS DYEALTLQAK VFQMPKSQEK
60 70 80 90 100
VAVGPAPQAV FSNSKQDPKE GVQILSYPRV TAKVKPQPSL QVWDKDSTYS
110 120 130 140 150
KLNPRLLKIW RNYLNMNKYK VSYKGPGPGV KFSVEALRCH LRDHVNVSMI
160 170 180 190 200
EATDFPFNTT EWEGYLPKEN FRTKAGPWHK CAVVSSAGSL KNSQLGREID
210 220 230 240 250
NHDAVLRFNG APTDNFQQDV GTKTTIRLVN SQLVTTEKRF LKDSLYTEGI
260 270 280 290 300
LILWDPSVYH ADIPQWYQKP DYNFFETYKS YRRLHPSQPF YILKPQMPWE
310 320 330 340 350
LWDIIQEISP DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC
360 370 380 390 400
YYHQKFFDSA CTMGAYHPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN

NRC
Length:403
Mass (Da):46,586
Last modified:July 27, 2011 - v2
Checksum:iABBD86D8B13D3E04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131K → R in BAA03680 (PubMed:8081843).Curated1
Sequence conflicti136A → G in BAA03680 (PubMed:8081843).Curated1
Sequence conflicti155F → S in BAA03680 (PubMed:8081843).Curated1
Sequence conflicti170N → T in BAA03680 (PubMed:8081843).Curated1
Sequence conflicti178 – 179WH → CT in BAA03680 (PubMed:8081843).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16106 mRNA Translation: BAA03680.1
AK084124 mRNA Translation: BAC39120.1
CH466521 Genomic DNA Translation: EDK97666.1
CH466521 Genomic DNA Translation: EDK97667.1
BC027833 mRNA Translation: AAH27833.1
BC092222 mRNA Translation: AAH92222.1
BC096026 mRNA Translation: AAH96026.1
CCDSiCCDS28077.1
RefSeqiNP_001239434.1, NM_001252505.1
NP_001239435.1, NM_001252506.1
NP_666045.1, NM_145933.4
XP_006521936.1, XM_006521873.3
XP_006521937.1, XM_006521874.1
XP_006521938.1, XM_006521875.3
XP_006521939.1, XM_006521876.3
XP_011244150.1, XM_011245848.1
UniGeneiMm.149029
Mm.489708

Genome annotation databases

EnsembliENSMUST00000023601; ENSMUSP00000023601; ENSMUSG00000022885
ENSMUST00000115335; ENSMUSP00000110992; ENSMUSG00000022885
ENSMUST00000178797; ENSMUSP00000136206; ENSMUSG00000022885
GeneIDi20440
KEGGimmu:20440
UCSCiuc007yto.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSIAT1_MOUSE
AccessioniPrimary (citable) accession number: Q64685
Secondary accession number(s): Q8K1L1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health