Reviewed, UniProtKB/Swiss-Prot Q64685 (SIAT1_MOUSE)

Names and origin

Protein names

Recommended name:
    Beta-galactoside alpha-2,6-sialyltransferase 1
    EC=2.4.99.1
Alternative name(s):
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
      Short name=Alpha 2,6-ST
      Short name=Sialyltransferase 1
    ST6Gal I

Gene names

Name:	St6gal1
Synonyms:	Siat1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactoside alpha-2,6-sialyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Beta-galactoside alpha-2,6-sialyltransferase 1		PRO_0000149250
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 26	17	Signal-anchor for type II membrane protein Potential
	Topological domain	27 – 403	377	Lumenal Potential
Amino acid modifications
	Glycosylation	146	1	N-linked (GlcNAc...) Potential
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Disulfide bond	181 ↔ 332		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64685-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: ECA8D0603A5A847B	FASTA	403	46,407
	10 20 30 40 50 60 MIHTNLKRKF SCFVLVFLLF AIICVWKKGS DYEALTLQAK VFQMPKSQEK VAVGPAPQAV 70 80 90 100 110 120 FSNSKQDPKE GVQILSYPRV TAKVKPQPSL QVWDKDSTYS KLNPRLLKIW RNYLNMNKYK 130 140 150 160 170 180 VSYKGPGPGV RFSVEGLRCH LRDHVNVSMI EATDSPFNTT EWEGYLPKET FRTKAGPCTK 190 200 210 220 230 240 CAVVSSAGSL KNSQLGREID NHDAVLRFNG APTDNFQQDV GTKTTIRLVN SQLVTTEKRF 250 260 270 280 290 300 LKDSLYTEGI LILWDPSVYH ADIPQWYQKP DYNFFETYKS YRRLHPSQPF YILKPQMPWE 310 320 330 340 350 360 LWDIIQEISP DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYHQKFFDSA 370 380 390 400 CTMGAYHPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN NRC

References

[1]	"Two step single primer mediated polymerase chain reaction. Application to cloning of putative mouse, beta-galactoside alpha 2,6-sialyltransferase cDNA." Hamamoto T., Kawasaki M., Kurosawa N., Nakaoka T., Lee Y.-C., Tsuji S. Bioorg. Med. Chem. 1:141-145(1993) [PubMed: 8081843] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain and Liver.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

ST6Gal I

Cross-references

Sequence databases
EMBLGenBankDDBJ	D16106 mRNA. Translation: BAA03680.1.
IPI	IPI00136942.
UniGene	Mm.149029
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q64685.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	Q64685.
Proteomic databases
PRIDE	Q64685.
Genome annotation databases
Ensembl	ENSMUST00000023601; ENSMUSP00000023601; ENSMUSG00000022885; Mus musculus. [Genome view] ENSMUST00000115335; ENSMUSP00000110992; ENSMUSG00000022885; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:108470. St6gal1.
Phylogenomic databases
HOGENOM	Q64685.
HOVERGEN	Q64685.
Enzyme and pathway databases
BRENDA	2.4.99.1. 244.
Gene expression databases
ArrayExpress	Q64685.
Bgee	Q64685.
CleanEx	MM_ST6GAL1.
Genevestigator	Q64685.
GermOnline	ENSMUSG00000022885. Mus musculus.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

SIAT1_MOUSE

Accession

Primary (citable) accession number: Q64685

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)