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Protein

Beta-galactoside alpha-2,6-sialyltransferase 1

Gene

St6gal1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186Substrate; via amide nitrogenBy similarity1
Binding sitei209SubstrateBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei350Substrate; via carbonyl oxygenBy similarity1
Binding sitei351SubstrateBy similarity1
Binding sitei362SubstrateBy similarity1
Binding sitei366SubstrateBy similarity1
Binding sitei367SubstrateBy similarity1
Binding sitei373SubstrateBy similarity1

GO - Molecular functioni

  • beta-galactoside alpha-2,6-sialyltransferase activity Source: MGI
  • sialyltransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.1. 3474.
ReactomeiR-MMU-4085001. Sialic acid metabolism.
R-MMU-975577. N-Glycan antennae elongation.
R-MMU-977068. Termination of O-glycan biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 1 (EC:2.4.99.1)
Short name:
Alpha 2,6-ST 1
Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
ST6Gal I
Short name:
ST6GalI
Sialyltransferase 1
Gene namesi
Name:St6gal1
Synonyms:Siat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:108470. St6gal1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini27 – 403LumenalSequence analysisAdd BLAST377

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492501 – 403Beta-galactoside alpha-2,6-sialyltransferase 1Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi139 ↔ 4031 Publication
Glycosylationi146N-linked (GlcNAc...)Sequence analysis1
Glycosylationi158N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi181 ↔ 3321 Publication
Disulfide bondi350 ↔ 3611 Publication
Modified residuei366PhosphotyrosineBy similarity1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ64685.
MaxQBiQ64685.
PaxDbiQ64685.
PeptideAtlasiQ64685.
PRIDEiQ64685.

PTM databases

iPTMnetiQ64685.
PhosphoSitePlusiQ64685.

Miscellaneous databases

PMAP-CutDBQ8K1L1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022885.
CleanExiMM_ST6GAL1.
ExpressionAtlasiQ64685. baseline and differential.
GenevisibleiQ64685. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023601.

Structurei

3D structure databases

ProteinModelPortaliQ64685.
SMRiQ64685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni319 – 321Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074444.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiQ64685.
KOiK00778.
OMAiSFQVWNK.
OrthoDBiEOG091G07C7.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q64685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHTNLKRKF SCFVLVFLLF AIICVWKKGS DYEALTLQAK VFQMPKSQEK
60 70 80 90 100
VAVGPAPQAV FSNSKQDPKE GVQILSYPRV TAKVKPQPSL QVWDKDSTYS
110 120 130 140 150
KLNPRLLKIW RNYLNMNKYK VSYKGPGPGV KFSVEALRCH LRDHVNVSMI
160 170 180 190 200
EATDFPFNTT EWEGYLPKEN FRTKAGPWHK CAVVSSAGSL KNSQLGREID
210 220 230 240 250
NHDAVLRFNG APTDNFQQDV GTKTTIRLVN SQLVTTEKRF LKDSLYTEGI
260 270 280 290 300
LILWDPSVYH ADIPQWYQKP DYNFFETYKS YRRLHPSQPF YILKPQMPWE
310 320 330 340 350
LWDIIQEISP DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC
360 370 380 390 400
YYHQKFFDSA CTMGAYHPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN

NRC
Length:403
Mass (Da):46,586
Last modified:July 27, 2011 - v2
Checksum:iABBD86D8B13D3E04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131K → R in BAA03680 (PubMed:8081843).Curated1
Sequence conflicti136A → G in BAA03680 (PubMed:8081843).Curated1
Sequence conflicti155F → S in BAA03680 (PubMed:8081843).Curated1
Sequence conflicti170N → T in BAA03680 (PubMed:8081843).Curated1
Sequence conflicti178 – 179WH → CT in BAA03680 (PubMed:8081843).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16106 mRNA. Translation: BAA03680.1.
AK084124 mRNA. Translation: BAC39120.1.
CH466521 Genomic DNA. Translation: EDK97666.1.
CH466521 Genomic DNA. Translation: EDK97667.1.
BC027833 mRNA. Translation: AAH27833.1.
BC092222 mRNA. Translation: AAH92222.1.
BC096026 mRNA. Translation: AAH96026.1.
CCDSiCCDS28077.1.
RefSeqiNP_001239434.1. NM_001252505.1.
NP_001239435.1. NM_001252506.1.
NP_666045.1. NM_145933.4.
XP_006521936.1. XM_006521873.3.
XP_006521937.1. XM_006521874.1.
XP_006521938.1. XM_006521875.3.
XP_006521939.1. XM_006521876.3.
XP_011244150.1. XM_011245848.1.
UniGeneiMm.149029.
Mm.489708.

Genome annotation databases

EnsembliENSMUST00000023601; ENSMUSP00000023601; ENSMUSG00000022885.
ENSMUST00000115335; ENSMUSP00000110992; ENSMUSG00000022885.
ENSMUST00000178797; ENSMUSP00000136206; ENSMUSG00000022885.
GeneIDi20440.
KEGGimmu:20440.
UCSCiuc007yto.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST6Gal I

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16106 mRNA. Translation: BAA03680.1.
AK084124 mRNA. Translation: BAC39120.1.
CH466521 Genomic DNA. Translation: EDK97666.1.
CH466521 Genomic DNA. Translation: EDK97667.1.
BC027833 mRNA. Translation: AAH27833.1.
BC092222 mRNA. Translation: AAH92222.1.
BC096026 mRNA. Translation: AAH96026.1.
CCDSiCCDS28077.1.
RefSeqiNP_001239434.1. NM_001252505.1.
NP_001239435.1. NM_001252506.1.
NP_666045.1. NM_145933.4.
XP_006521936.1. XM_006521873.3.
XP_006521937.1. XM_006521874.1.
XP_006521938.1. XM_006521875.3.
XP_006521939.1. XM_006521876.3.
XP_011244150.1. XM_011245848.1.
UniGeneiMm.149029.
Mm.489708.

3D structure databases

ProteinModelPortaliQ64685.
SMRiQ64685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023601.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiQ64685.
PhosphoSitePlusiQ64685.

Proteomic databases

EPDiQ64685.
MaxQBiQ64685.
PaxDbiQ64685.
PeptideAtlasiQ64685.
PRIDEiQ64685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023601; ENSMUSP00000023601; ENSMUSG00000022885.
ENSMUST00000115335; ENSMUSP00000110992; ENSMUSG00000022885.
ENSMUST00000178797; ENSMUSP00000136206; ENSMUSG00000022885.
GeneIDi20440.
KEGGimmu:20440.
UCSCiuc007yto.2. mouse.

Organism-specific databases

CTDi6480.
MGIiMGI:108470. St6gal1.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074444.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiQ64685.
KOiK00778.
OMAiSFQVWNK.
OrthoDBiEOG091G07C7.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.1. 3474.
ReactomeiR-MMU-4085001. Sialic acid metabolism.
R-MMU-975577. N-Glycan antennae elongation.
R-MMU-977068. Termination of O-glycan biosynthesis.

Miscellaneous databases

ChiTaRSiSt6gal1. mouse.
PMAP-CutDBQ8K1L1.
PROiQ64685.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022885.
CleanExiMM_ST6GAL1.
ExpressionAtlasiQ64685. baseline and differential.
GenevisibleiQ64685. MM.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIAT1_MOUSE
AccessioniPrimary (citable) accession number: Q64685
Secondary accession number(s): Q8K1L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.