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Protein

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Gene

Ugt8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.

Catalytic activityi

UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.

Pathwayi

GO - Molecular functioni

  1. 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity Source: UniProtKB-EC
  2. ceramide glucosyltransferase activity Source: MGI
  3. glucuronosyltransferase activity Source: GO_Central
  4. UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Source: Ensembl

GO - Biological processi

  1. axon cargo transport Source: BHF-UCL
  2. cellular response to hormone stimulus Source: GO_Central
  3. cytoskeleton organization Source: BHF-UCL
  4. flavonoid biosynthetic process Source: GO_Central
  5. flavonoid glucuronidation Source: GO_Central
  6. galactosylceramide biosynthetic process Source: UniProtKB-UniPathway
  7. glycolipid biosynthetic process Source: MGI
  8. myelination Source: MGI
  9. neuron projection morphogenesis Source: BHF-UCL
  10. paranodal junction assembly Source: BHF-UCL
  11. protein localization to paranode region of axon Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00787.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase (EC:2.4.1.45)
Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase
Gene namesi
Name:Ugt8
Synonyms:Cgt, Ugt4, Ugt8a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:109522. Ugt8a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei472 – 49221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 5415212-hydroxyacylsphingosine 1-beta-galactosyltransferasePRO_0000036065Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi442 – 4421N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ64676.
PaxDbiQ64676.
PRIDEiQ64676.

PTM databases

PhosphoSiteiQ64676.

Expressioni

Gene expression databases

BgeeiQ64676.
CleanExiMM_UGT8A.
GenevestigatoriQ64676.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050852.

Structurei

3D structure databases

ProteinModelPortaliQ64676.
SMRiQ64676. Positions 218-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG098341.
InParanoidiQ64676.
KOiK04628.
OMAiSNHYSLQ.
OrthoDBiEOG7GBFWS.
PhylomeDBiQ64676.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64676-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH
60 70 80 90 100
HTVLLLSEGR DIAPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA
110 120 130 140 150
VELVDILDHY TKNCDMMVGN QALIQGLKKE KFDLLLVDPN DMCGFVIAHL
160 170 180 190 200
LGVKYAVFST GLWYPAEVGA PAPLAYVPEF NSLLTDRMNF LERMKNTGVY
210 220 230 240 250
LISRIGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW MLCTDVALEF
260 270 280 290 300
PRPTLPNVVY VGGILTKPAS PLPEDLQRWV SGAQEHGFVL VSFGAGVKYL
310 320 330 340 350
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS
360 370 380 390 400
NIRAFLSHGG LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE
410 420 430 440 450
WNTVTEGELY DALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY
460 470 480 490 500
ILRHDGARHL RSAVHQISFC QYFLLDIAFV LLLGAVLLYF ILSYVTKFIY
510 520 530 540
RKIKSLWSKN EHSTVNGHYQ NGIRNGKYKG NGRVKHEKKV R
Length:541
Mass (Da):61,249
Last modified:February 21, 2006 - v2
Checksum:i0E3436597F8810D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti335 – 3351T → S in AAC53576. (PubMed:8661123)Curated
Sequence conflicti458 – 4581R → H in CAA63090. (PubMed:8661124)Curated
Sequence conflicti458 – 4581R → H in CAA63091. (PubMed:8661124)Curated
Sequence conflicti511 – 5111E → K in CAA63090. (PubMed:8661124)Curated
Sequence conflicti511 – 5111E → K in CAA63091. (PubMed:8661124)Curated
Sequence conflicti524 – 5241R → P in CAA63090. (PubMed:8661124)Curated
Sequence conflicti524 – 5241R → P in CAA63091. (PubMed:8661124)Curated
Sequence conflicti533 – 5342RV → HI in CAA63090. (PubMed:8661124)Curated
Sequence conflicti533 – 5342RV → HI in CAA63091. (PubMed:8661124)Curated
Sequence conflicti541 – 5411R → K in CAA63090. (PubMed:8661124)Curated
Sequence conflicti541 – 5411R → K in CAA63091. (PubMed:8661124)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48896
, U48892, U48893, U48894 Genomic DNA. Translation: AAC53576.1.
X92122 mRNA. Translation: CAA63090.1.
X92123
, X92124, X92125, X92126, X92177 Genomic DNA. Translation: CAA63091.1.
AK137364 mRNA. Translation: BAE23325.1.
BC016885 mRNA. Translation: AAH16885.1.
CCDSiCCDS17821.1.
RefSeqiNP_035804.2. NM_011674.4.
XP_006501373.1. XM_006501310.1.
UniGeneiMm.306021.

Genome annotation databases

EnsembliENSMUST00000057944; ENSMUSP00000050852; ENSMUSG00000032854.
GeneIDi22239.
KEGGimmu:22239.
UCSCiuc008rfy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48896
, U48892, U48893, U48894 Genomic DNA. Translation: AAC53576.1.
X92122 mRNA. Translation: CAA63090.1.
X92123
, X92124, X92125, X92126, X92177 Genomic DNA. Translation: CAA63091.1.
AK137364 mRNA. Translation: BAE23325.1.
BC016885 mRNA. Translation: AAH16885.1.
CCDSiCCDS17821.1.
RefSeqiNP_035804.2. NM_011674.4.
XP_006501373.1. XM_006501310.1.
UniGeneiMm.306021.

3D structure databases

ProteinModelPortaliQ64676.
SMRiQ64676. Positions 218-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050852.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteiQ64676.

Proteomic databases

MaxQBiQ64676.
PaxDbiQ64676.
PRIDEiQ64676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057944; ENSMUSP00000050852; ENSMUSG00000032854.
GeneIDi22239.
KEGGimmu:22239.
UCSCiuc008rfy.1. mouse.

Organism-specific databases

CTDi22239.
MGIiMGI:109522. Ugt8a.

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG098341.
InParanoidiQ64676.
KOiK04628.
OMAiSNHYSLQ.
OrthoDBiEOG7GBFWS.
PhylomeDBiQ64676.
TreeFamiTF315472.

Enzyme and pathway databases

UniPathwayiUPA00787.

Miscellaneous databases

NextBioi302297.
PROiQ64676.
SOURCEiSearch...

Gene expression databases

BgeeiQ64676.
CleanExiMM_UGT8A.
GenevestigatoriQ64676.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, chromosomal mapping, and characterization of the mouse UDP-galactose:ceramide galactosyltransferase gene."
    Coetzee T., Li X., Fujita N., Marcus J., Suzuki K., Francke U., Popko B.
    Genomics 35:215-222(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning and characterization of the mouse CGT gene encoding UDP-galactose ceramide-galactosyltransferase (cerebroside synthetase)."
    Bosio A., Binczek E., Stoffel W.
    Genomics 35:223-226(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: BALB/c.
    Tissue: Leukocyte.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.

Entry informationi

Entry nameiCGT_MOUSE
AccessioniPrimary (citable) accession number: Q64676
Secondary accession number(s): Q61634, Q91W57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 21, 2006
Last modified: February 4, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.