Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q64676 (CGT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase

EC=2.4.1.45
Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase
Gene names
Name:Ugt8
Synonyms:Cgt, Ugt4, Ugt8a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.

Catalytic activity

UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.

Pathway

Sphingolipid metabolism; galactosylceramide biosynthesis.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
   Biological processLipid metabolism
Sphingolipid metabolism
   Cellular componentMembrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon cargo transport

Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL

cytoskeleton organization

Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL

galactosylceramide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

glycolipid biosynthetic process

Traceable author statement PubMed 11917099. Source: MGI

myelination

Traceable author statement PubMed 11917099. Source: MGI

neuron projection morphogenesis

Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL

paranodal junction assembly

Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL

protein localization to paranode region of axon

Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_function2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity

Inferred from electronic annotation. Source: Ensembl

ceramide glucosyltransferase activity

Traceable author statement PubMed 11917099. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 5415212-hydroxyacylsphingosine 1-beta-galactosyltransferase
PRO_0000036065

Regions

Transmembrane472 – 49221Helical; Potential

Amino acid modifications

Glycosylation781N-linked (GlcNAc...) Potential
Glycosylation3331N-linked (GlcNAc...) Potential
Glycosylation4421N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3351T → S in AAC53576. Ref.1
Sequence conflict4581R → H in CAA63090. Ref.2
Sequence conflict4581R → H in CAA63091. Ref.2
Sequence conflict5111E → K in CAA63090. Ref.2
Sequence conflict5111E → K in CAA63091. Ref.2
Sequence conflict5241R → P in CAA63090. Ref.2
Sequence conflict5241R → P in CAA63091. Ref.2
Sequence conflict533 – 5342RV → HI in CAA63090. Ref.2
Sequence conflict533 – 5342RV → HI in CAA63091. Ref.2
Sequence conflict5411R → K in CAA63090. Ref.2
Sequence conflict5411R → K in CAA63091. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q64676 [UniParc].

Last modified February 21, 2006. Version 2.
Checksum: 0E3436597F8810D0

FASTA54161,249
        10         20         30         40         50         60 
MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH HTVLLLSEGR 

        70         80         90        100        110        120 
DIAPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA VELVDILDHY TKNCDMMVGN 

       130        140        150        160        170        180 
QALIQGLKKE KFDLLLVDPN DMCGFVIAHL LGVKYAVFST GLWYPAEVGA PAPLAYVPEF 

       190        200        210        220        230        240 
NSLLTDRMNF LERMKNTGVY LISRIGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW 

       250        260        270        280        290        300 
MLCTDVALEF PRPTLPNVVY VGGILTKPAS PLPEDLQRWV SGAQEHGFVL VSFGAGVKYL 

       310        320        330        340        350        360 
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS NIRAFLSHGG 

       370        380        390        400        410        420 
LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE WNTVTEGELY DALVKVINNP 

       430        440        450        460        470        480 
SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY ILRHDGARHL RSAVHQISFC QYFLLDIAFV 

       490        500        510        520        530        540 
LLLGAVLLYF ILSYVTKFIY RKIKSLWSKN EHSTVNGHYQ NGIRNGKYKG NGRVKHEKKV 


R 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, chromosomal mapping, and characterization of the mouse UDP-galactose:ceramide galactosyltransferase gene."
Coetzee T., Li X., Fujita N., Marcus J., Suzuki K., Francke U., Popko B.
Genomics 35:215-222(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular cloning and characterization of the mouse CGT gene encoding UDP-galactose ceramide-galactosyltransferase (cerebroside synthetase)."
Bosio A., Binczek E., Stoffel W.
Genomics 35:223-226(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: BALB/c.
Tissue: Leukocyte.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cerebellum.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U48896 expand/collapse EMBL AC list , U48892, U48893, U48894 Genomic DNA. Translation: AAC53576.1.
X92122 mRNA. Translation: CAA63090.1.
X92123 expand/collapse EMBL AC list , X92124, X92125, X92126, X92177 Genomic DNA. Translation: CAA63091.1.
AK137364 mRNA. Translation: BAE23325.1.
BC016885 mRNA. Translation: AAH16885.1.
RefSeqNP_035804.2. NM_011674.4.
XP_006501373.1. XM_006501310.1.
UniGeneMm.306021.

3D structure databases

ProteinModelPortalQ64676.
SMRQ64676. Positions 218-430.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000050852.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteQ64676.

Proteomic databases

PaxDbQ64676.
PRIDEQ64676.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000057944; ENSMUSP00000050852; ENSMUSG00000032854.
GeneID22239.
KEGGmmu:22239.
UCSCuc008rfy.1. mouse.

Organism-specific databases

CTD22239.
MGIMGI:109522. Ugt8a.

Phylogenomic databases

eggNOGCOG1819.
GeneTreeENSGT00560000076760.
HOGENOMHOG000220831.
HOVERGENHBG098341.
InParanoidQ64676.
KOK04628.
OMASNHYSLQ.
OrthoDBEOG7GBFWS.
PhylomeDBQ64676.
TreeFamTF315472.

Enzyme and pathway databases

UniPathwayUPA00787.

Gene expression databases

BgeeQ64676.
CleanExMM_UGT8A.
GenevestigatorQ64676.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302297.
PROQ64676.
SOURCESearch...

Entry information

Entry nameCGT_MOUSE
AccessionPrimary (citable) accession number: Q64676
Secondary accession number(s): Q61634, Q91W57
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 21, 2006
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot