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Protein

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Gene

Ugt8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acylsphingosine = UDP + beta-D-galactosylceramide.By similarity

Pathwayi: galactosylceramide biosynthesis

This protein is involved in the pathway galactosylceramide biosynthesis, which is part of Sphingolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway galactosylceramide biosynthesis and in Sphingolipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • cytoskeleton organization Source: BHF-UCL
  • galactosylceramide biosynthetic process Source: UniProtKB-UniPathway
  • glycolipid biosynthetic process Source: MGI
  • myelination Source: MGI
  • neuron projection morphogenesis Source: BHF-UCL
  • paranodal junction assembly Source: BHF-UCL
  • protein localization to paranode region of axon Source: BHF-UCL

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
UniPathwayiUPA00787.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase (EC:2.4.1.47By similarity)
Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase
Gene namesi
Name:Ugt8
Synonyms:Cgt, Ugt4, Ugt8a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109522. Ugt8a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei472 – 492HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003606521 – 5412-hydroxyacylsphingosine 1-beta-galactosyltransferaseAdd BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi78N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi333N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi442N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ64676.
PaxDbiQ64676.
PeptideAtlasiQ64676.
PRIDEiQ64676.

PTM databases

iPTMnetiQ64676.
PhosphoSitePlusiQ64676.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032854.
CleanExiMM_UGT8A.
GenevisibleiQ64676. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050852.

Structurei

3D structure databases

ProteinModelPortaliQ64676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG098341.
InParanoidiQ64676.
KOiK04628.
OMAiIAPSNHY.
OrthoDBiEOG091G06JC.
PhylomeDBiQ64676.
TreeFamiTF315472.

Family and domain databases

InterProiView protein in InterPro
IPR002213. UDP_glucos_trans.
IPR035595. UDP_glycos_trans_CS.
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiView protein in Pfam
PF00201. UDPGT. 1 hit.
PROSITEiView protein in PROSITE
PS00375. UDPGT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH
60 70 80 90 100
HTVLLLSEGR DIAPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA
110 120 130 140 150
VELVDILDHY TKNCDMMVGN QALIQGLKKE KFDLLLVDPN DMCGFVIAHL
160 170 180 190 200
LGVKYAVFST GLWYPAEVGA PAPLAYVPEF NSLLTDRMNF LERMKNTGVY
210 220 230 240 250
LISRIGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW MLCTDVALEF
260 270 280 290 300
PRPTLPNVVY VGGILTKPAS PLPEDLQRWV SGAQEHGFVL VSFGAGVKYL
310 320 330 340 350
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS
360 370 380 390 400
NIRAFLSHGG LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE
410 420 430 440 450
WNTVTEGELY DALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY
460 470 480 490 500
ILRHDGARHL RSAVHQISFC QYFLLDIAFV LLLGAVLLYF ILSYVTKFIY
510 520 530 540
RKIKSLWSKN EHSTVNGHYQ NGIRNGKYKG NGRVKHEKKV R
Length:541
Mass (Da):61,249
Last modified:February 21, 2006 - v2
Checksum:i0E3436597F8810D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti335T → S in AAC53576 (PubMed:8661123).Curated1
Sequence conflicti458R → H in CAA63090 (PubMed:8661124).Curated1
Sequence conflicti458R → H in CAA63091 (PubMed:8661124).Curated1
Sequence conflicti511E → K in CAA63090 (PubMed:8661124).Curated1
Sequence conflicti511E → K in CAA63091 (PubMed:8661124).Curated1
Sequence conflicti524R → P in CAA63090 (PubMed:8661124).Curated1
Sequence conflicti524R → P in CAA63091 (PubMed:8661124).Curated1
Sequence conflicti533 – 534RV → HI in CAA63090 (PubMed:8661124).Curated2
Sequence conflicti533 – 534RV → HI in CAA63091 (PubMed:8661124).Curated2
Sequence conflicti541R → K in CAA63090 (PubMed:8661124).Curated1
Sequence conflicti541R → K in CAA63091 (PubMed:8661124).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48896
, U48892, U48893, U48894 Genomic DNA. Translation: AAC53576.1.
X92122 mRNA. Translation: CAA63090.1.
X92123
, X92124, X92125, X92126, X92177 Genomic DNA. Translation: CAA63091.1.
AK137364 mRNA. Translation: BAE23325.1.
BC016885 mRNA. Translation: AAH16885.1.
CCDSiCCDS17821.1.
RefSeqiNP_035804.2. NM_011674.4.
XP_006501372.2. XM_006501309.3.
XP_006501373.1. XM_006501310.3.
UniGeneiMm.306021.

Genome annotation databases

EnsembliENSMUST00000057944; ENSMUSP00000050852; ENSMUSG00000032854.
ENSMUST00000198610; ENSMUSP00000143605; ENSMUSG00000032854.
GeneIDi22239.
KEGGimmu:22239.
UCSCiuc008rfy.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCGT_MOUSE
AccessioniPrimary (citable) accession number: Q64676
Secondary accession number(s): Q61634, Q91W57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 21, 2006
Last modified: August 30, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families