2-hydroxyacylsphingosine 1-beta-galactosyltransferase precursor

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Q64676 (CGT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
EC=2.4.1.45

Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase

Gene names

Name:	Ugt8
Synonyms:	Cgt, Ugt4, Ugt8a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.
Catalytic activity	UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.
Pathway	Sphingolipid metabolism; galactosylceramide biosynthesis.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon cargo transport Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL cytoskeleton organization Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL galactosylceramide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glycolipid biosynthetic process Traceable author statement PubMed 11917099. Source: MGI neuron projection morphogenesis Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL paranodal junction assembly Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL protein localization to paranode region of axon Inferred from mutant phenotype PubMed 16551741. Source: BHF-UCL
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity Inferred from electronic annotation. Source: EC UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Inferred from electronic annotation. Source: Compara ceramide glucosyltransferase activity Traceable author statement PubMed 11917099. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

Potential

Chain

21 – 541

521

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

PRO_0000036065

Regions

Transmembrane

472 – 492

Helical; Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

333

N-linked (GlcNAc...) Potential

Glycosylation

442

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

335

T → S in AAC53576. Ref.1

Sequence conflict

458

R → H in CAA63090. Ref.2

Sequence conflict

458

R → H in CAA63091. Ref.2

Sequence conflict

511

E → K in CAA63090. Ref.2

Sequence conflict

511

E → K in CAA63091. Ref.2

Sequence conflict

524

R → P in CAA63090. Ref.2

Sequence conflict

524

R → P in CAA63091. Ref.2

Sequence conflict

533 – 534

RV → HI in CAA63090. Ref.2

Sequence conflict

533 – 534

RV → HI in CAA63091. Ref.2

Sequence conflict

541

R → K in CAA63090. Ref.2

Sequence conflict

541

R → K in CAA63091. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q64676 [UniParc].

Last modified February 21, 2006. Version 2.
Checksum: 0E3436597F8810D0
Show »

FASTA

541

61,249

        10         20         30         40         50         60 
MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH HTVLLLSEGR 

        70         80         90        100        110        120 
DIAPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA VELVDILDHY TKNCDMMVGN 

       130        140        150        160        170        180 
QALIQGLKKE KFDLLLVDPN DMCGFVIAHL LGVKYAVFST GLWYPAEVGA PAPLAYVPEF 

       190        200        210        220        230        240 
NSLLTDRMNF LERMKNTGVY LISRIGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW 

       250        260        270        280        290        300 
MLCTDVALEF PRPTLPNVVY VGGILTKPAS PLPEDLQRWV SGAQEHGFVL VSFGAGVKYL 

       310        320        330        340        350        360 
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS NIRAFLSHGG 

       370        380        390        400        410        420 
LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE WNTVTEGELY DALVKVINNP 

       430        440        450        460        470        480 
SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY ILRHDGARHL RSAVHQISFC QYFLLDIAFV 

       490        500        510        520        530        540 
LLLGAVLLYF ILSYVTKFIY RKIKSLWSKN EHSTVNGHYQ NGIRNGKYKG NGRVKHEKKV 


R

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, chromosomal mapping, and characterization of the mouse UDP-galactose:ceramide galactosyltransferase gene." Coetzee T., Li X., Fujita N., Marcus J., Suzuki K., Francke U., Popko B. Genomics 35:215-222(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and characterization of the mouse CGT gene encoding UDP-galactose ceramide-galactosyltransferase (cerebroside synthetase)." Bosio A., Binczek E., Stoffel W. Genomics 35:223-226(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: BALB/c. Tissue: Leukocyte.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U48896 , U48892, U48893, U48894 Genomic DNA. Translation: AAC53576.1. X92122 mRNA. Translation: CAA63090.1. X92123 , X92124, X92125, X92126, X92177 Genomic DNA. Translation: CAA63091.1. AK137364 mRNA. Translation: BAE23325.1. BC016885 mRNA. Translation: AAH16885.1.
IPI	IPI00136915.
RefSeq	NP_035804.2. NM_011674.4.
UniGene	Mm.306021.
3D structure databases
ProteinModelPortal	Q64676.
SMR	Q64676. Positions 218-430.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000050852.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
PTM databases
PhosphoSite	Q64676.
Proteomic databases
PaxDb	Q64676.
PRIDE	Q64676.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000057944; ENSMUSP00000050852; ENSMUSG00000032854.
GeneID	22239.
KEGG	mmu:22239.
UCSC	uc008rfy.1. mouse.
Organism-specific databases
CTD	22239.
MGI	MGI:109522. Ugt8a.
Phylogenomic databases
eggNOG	COG1819.
GeneTree	ENSGT00560000076760.
HOGENOM	HOG000220831.
HOVERGEN	HBG098341.
InParanoid	Q64676.
KO	K04628.
OMA	NHYSLQR.
OrthoDB	EOG4KKZ2Q.
Enzyme and pathway databases
UniPathway	UPA00787.
Gene expression databases
Bgee	Q64676.
CleanEx	MM_UGT8A.
Genevestigator	Q64676.
GermOnline	ENSMUSG00000032854. Mus musculus.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302297.
SOURCE	Search...

Entry information

Entry name

CGT_MOUSE

Accession

Primary (citable) accession number: Q64676
Secondary accession number(s): Q61634, Q91W57

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 21, 2006
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents