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Protein

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Gene

Ugt8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acylsphingosine = UDP + beta-D-galactosylceramide.By similarity

Pathwayi: galactosylceramide biosynthesis

This protein is involved in the pathway galactosylceramide biosynthesis, which is part of Sphingolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway galactosylceramide biosynthesis and in Sphingolipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • cytoskeleton organization Source: BHF-UCL
  • galactosylceramide biosynthetic process Source: UniProtKB-UniPathway
  • glycolipid biosynthetic process Source: MGI
  • myelination Source: MGI
  • neuron projection morphogenesis Source: BHF-UCL
  • paranodal junction assembly Source: BHF-UCL
  • protein localization to paranode region of axon Source: BHF-UCL

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1660662 Glycosphingolipid metabolism
UniPathwayiUPA00787

Protein family/group databases

CAZyiGT1 Glycosyltransferase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase (EC:2.4.1.47By similarity)
Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase
Gene namesi
Name:Ugt8
Synonyms:Cgt, Ugt4, Ugt8a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109522 Ugt8a

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei472 – 492HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003606521 – 5412-hydroxyacylsphingosine 1-beta-galactosyltransferaseAdd BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi78N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi333N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi442N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ64676
PaxDbiQ64676
PeptideAtlasiQ64676
PRIDEiQ64676

PTM databases

iPTMnetiQ64676
PhosphoSitePlusiQ64676

Expressioni

Gene expression databases

BgeeiENSMUSG00000032854
CleanExiMM_UGT8A
GenevisibleiQ64676 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050852

Structurei

3D structure databases

ProteinModelPortaliQ64676
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1192 Eukaryota
COG1819 LUCA
GeneTreeiENSGT00760000118949
HOGENOMiHOG000220831
HOVERGENiHBG098341
InParanoidiQ64676
KOiK04628
OMAiIAPSNHY
OrthoDBiEOG091G06JC
PhylomeDBiQ64676
TreeFamiTF315472

Family and domain databases

InterProiView protein in InterPro
IPR002213 UDP_glucos_trans
IPR035595 UDP_glycos_trans_CS
PfamiView protein in Pfam
PF00201 UDPGT, 1 hit
PROSITEiView protein in PROSITE
PS00375 UDPGT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH
60 70 80 90 100
HTVLLLSEGR DIAPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA
110 120 130 140 150
VELVDILDHY TKNCDMMVGN QALIQGLKKE KFDLLLVDPN DMCGFVIAHL
160 170 180 190 200
LGVKYAVFST GLWYPAEVGA PAPLAYVPEF NSLLTDRMNF LERMKNTGVY
210 220 230 240 250
LISRIGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW MLCTDVALEF
260 270 280 290 300
PRPTLPNVVY VGGILTKPAS PLPEDLQRWV SGAQEHGFVL VSFGAGVKYL
310 320 330 340 350
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS
360 370 380 390 400
NIRAFLSHGG LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE
410 420 430 440 450
WNTVTEGELY DALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY
460 470 480 490 500
ILRHDGARHL RSAVHQISFC QYFLLDIAFV LLLGAVLLYF ILSYVTKFIY
510 520 530 540
RKIKSLWSKN EHSTVNGHYQ NGIRNGKYKG NGRVKHEKKV R
Length:541
Mass (Da):61,249
Last modified:February 21, 2006 - v2
Checksum:i0E3436597F8810D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti335T → S in AAC53576 (PubMed:8661123).Curated1
Sequence conflicti458R → H in CAA63090 (PubMed:8661124).Curated1
Sequence conflicti458R → H in CAA63091 (PubMed:8661124).Curated1
Sequence conflicti511E → K in CAA63090 (PubMed:8661124).Curated1
Sequence conflicti511E → K in CAA63091 (PubMed:8661124).Curated1
Sequence conflicti524R → P in CAA63090 (PubMed:8661124).Curated1
Sequence conflicti524R → P in CAA63091 (PubMed:8661124).Curated1
Sequence conflicti533 – 534RV → HI in CAA63090 (PubMed:8661124).Curated2
Sequence conflicti533 – 534RV → HI in CAA63091 (PubMed:8661124).Curated2
Sequence conflicti541R → K in CAA63090 (PubMed:8661124).Curated1
Sequence conflicti541R → K in CAA63091 (PubMed:8661124).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48896
, U48892, U48893, U48894 Genomic DNA Translation: AAC53576.1
X92122 mRNA Translation: CAA63090.1
X92123
, X92124, X92125, X92126, X92177 Genomic DNA Translation: CAA63091.1
AK137364 mRNA Translation: BAE23325.1
BC016885 mRNA Translation: AAH16885.1
CCDSiCCDS17821.1
RefSeqiNP_035804.2, NM_011674.4
XP_006501372.2, XM_006501309.3
XP_006501373.1, XM_006501310.3
UniGeneiMm.306021

Genome annotation databases

EnsembliENSMUST00000057944; ENSMUSP00000050852; ENSMUSG00000032854
ENSMUST00000198610; ENSMUSP00000143605; ENSMUSG00000032854
GeneIDi22239
KEGGimmu:22239
UCSCiuc008rfy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCGT_MOUSE
AccessioniPrimary (citable) accession number: Q64676
Secondary accession number(s): Q61634, Q91W57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 21, 2006
Last modified: December 20, 2017
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health