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Q64676

- CGT_MOUSE

UniProt

Q64676 - CGT_MOUSE

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Protein

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Gene
Ugt8, Cgt, Ugt4, Ugt8a
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.

Catalytic activityi

UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.

Pathwayi

GO - Molecular functioni

  1. 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity Source: UniProtKB-EC
  2. ceramide glucosyltransferase activity Source: MGI
  3. UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Source: Ensembl

GO - Biological processi

  1. axon cargo transport Source: BHF-UCL
  2. cytoskeleton organization Source: BHF-UCL
  3. galactosylceramide biosynthetic process Source: UniProtKB-UniPathway
  4. glycolipid biosynthetic process Source: MGI
  5. myelination Source: MGI
  6. neuron projection morphogenesis Source: BHF-UCL
  7. paranodal junction assembly Source: BHF-UCL
  8. protein localization to paranode region of axon Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00787.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase (EC:2.4.1.45)
Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase
Gene namesi
Name:Ugt8
Synonyms:Cgt, Ugt4, Ugt8a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:109522. Ugt8a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei472 – 49221Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 5415212-hydroxyacylsphingosine 1-beta-galactosyltransferasePRO_0000036065Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...) Reviewed prediction
Glycosylationi333 – 3331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi442 – 4421N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ64676.
PRIDEiQ64676.

PTM databases

PhosphoSiteiQ64676.

Expressioni

Gene expression databases

BgeeiQ64676.
CleanExiMM_UGT8A.
GenevestigatoriQ64676.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050852.

Structurei

3D structure databases

ProteinModelPortaliQ64676.
SMRiQ64676. Positions 218-430.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00560000076760.
HOGENOMiHOG000220831.
HOVERGENiHBG098341.
InParanoidiQ64676.
KOiK04628.
OMAiSNHYSLQ.
OrthoDBiEOG7GBFWS.
PhylomeDBiQ64676.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64676-1 [UniParc]FASTAAdd to Basket

« Hide

MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH    50
HTVLLLSEGR DIAPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA 100
VELVDILDHY TKNCDMMVGN QALIQGLKKE KFDLLLVDPN DMCGFVIAHL 150
LGVKYAVFST GLWYPAEVGA PAPLAYVPEF NSLLTDRMNF LERMKNTGVY 200
LISRIGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW MLCTDVALEF 250
PRPTLPNVVY VGGILTKPAS PLPEDLQRWV SGAQEHGFVL VSFGAGVKYL 300
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS 350
NIRAFLSHGG LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE 400
WNTVTEGELY DALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY 450
ILRHDGARHL RSAVHQISFC QYFLLDIAFV LLLGAVLLYF ILSYVTKFIY 500
RKIKSLWSKN EHSTVNGHYQ NGIRNGKYKG NGRVKHEKKV R 541
Length:541
Mass (Da):61,249
Last modified:February 21, 2006 - v2
Checksum:i0E3436597F8810D0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti335 – 3351T → S in AAC53576. 1 Publication
Sequence conflicti458 – 4581R → H in CAA63090. 1 Publication
Sequence conflicti458 – 4581R → H in CAA63091. 1 Publication
Sequence conflicti511 – 5111E → K in CAA63090. 1 Publication
Sequence conflicti511 – 5111E → K in CAA63091. 1 Publication
Sequence conflicti524 – 5241R → P in CAA63090. 1 Publication
Sequence conflicti524 – 5241R → P in CAA63091. 1 Publication
Sequence conflicti533 – 5342RV → HI in CAA63090. 1 Publication
Sequence conflicti533 – 5342RV → HI in CAA63091. 1 Publication
Sequence conflicti541 – 5411R → K in CAA63090. 1 Publication
Sequence conflicti541 – 5411R → K in CAA63091. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48896
, U48892, U48893, U48894 Genomic DNA. Translation: AAC53576.1.
X92122 mRNA. Translation: CAA63090.1.
X92123
, X92124, X92125, X92126, X92177 Genomic DNA. Translation: CAA63091.1.
AK137364 mRNA. Translation: BAE23325.1.
BC016885 mRNA. Translation: AAH16885.1.
CCDSiCCDS17821.1.
RefSeqiNP_035804.2. NM_011674.4.
XP_006501373.1. XM_006501310.1.
UniGeneiMm.306021.

Genome annotation databases

EnsembliENSMUST00000057944; ENSMUSP00000050852; ENSMUSG00000032854.
GeneIDi22239.
KEGGimmu:22239.
UCSCiuc008rfy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48896
, U48892 , U48893 , U48894 Genomic DNA. Translation: AAC53576.1 .
X92122 mRNA. Translation: CAA63090.1 .
X92123
, X92124 , X92125 , X92126 , X92177 Genomic DNA. Translation: CAA63091.1 .
AK137364 mRNA. Translation: BAE23325.1 .
BC016885 mRNA. Translation: AAH16885.1 .
CCDSi CCDS17821.1.
RefSeqi NP_035804.2. NM_011674.4.
XP_006501373.1. XM_006501310.1.
UniGenei Mm.306021.

3D structure databases

ProteinModelPortali Q64676.
SMRi Q64676. Positions 218-430.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000050852.

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSitei Q64676.

Proteomic databases

PaxDbi Q64676.
PRIDEi Q64676.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000057944 ; ENSMUSP00000050852 ; ENSMUSG00000032854 .
GeneIDi 22239.
KEGGi mmu:22239.
UCSCi uc008rfy.1. mouse.

Organism-specific databases

CTDi 22239.
MGIi MGI:109522. Ugt8a.

Phylogenomic databases

eggNOGi COG1819.
GeneTreei ENSGT00560000076760.
HOGENOMi HOG000220831.
HOVERGENi HBG098341.
InParanoidi Q64676.
KOi K04628.
OMAi SNHYSLQ.
OrthoDBi EOG7GBFWS.
PhylomeDBi Q64676.
TreeFami TF315472.

Enzyme and pathway databases

UniPathwayi UPA00787 .

Miscellaneous databases

NextBioi 302297.
PROi Q64676.
SOURCEi Search...

Gene expression databases

Bgeei Q64676.
CleanExi MM_UGT8A.
Genevestigatori Q64676.

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, chromosomal mapping, and characterization of the mouse UDP-galactose:ceramide galactosyltransferase gene."
    Coetzee T., Li X., Fujita N., Marcus J., Suzuki K., Francke U., Popko B.
    Genomics 35:215-222(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning and characterization of the mouse CGT gene encoding UDP-galactose ceramide-galactosyltransferase (cerebroside synthetase)."
    Bosio A., Binczek E., Stoffel W.
    Genomics 35:223-226(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: BALB/c.
    Tissue: Leukocyte.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.

Entry informationi

Entry nameiCGT_MOUSE
AccessioniPrimary (citable) accession number: Q64676
Secondary accession number(s): Q61634, Q91W57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 21, 2006
Last modified: July 9, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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