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Protein

Spermidine synthase

Gene

Srm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine (By similarity).By similarity

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.

Enzyme regulationi

The activity is thought to be regulated mainly by the availability of decarboxylated S-adenosylmethionine.

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.
Proteins known to be involved in this subpathway in this organism are:
  1. Spermidine synthase (Srm)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49S-adenosylmethioninamineBy similarity1
Binding sitei79PutrescineBy similarity1
Binding sitei80S-adenosylmethioninamineBy similarity1
Binding sitei104S-adenosylmethioninamineBy similarity1
Binding sitei124S-adenosylmethioninamineBy similarity1
Active sitei173Proton acceptorBy similarity1
Binding sitei173S-adenosylmethioninamineBy similarity1
Binding sitei241PutrescineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPolyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-351202 Metabolism of polyamines
UniPathwayiUPA00248; UER00314

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine synthase (EC:2.5.1.16)
Short name:
SPDSY
Alternative name(s):
Putrescine aminopropyltransferase
Gene namesi
Name:Srm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:102690 Srm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564461 – 302Spermidine synthaseAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ64674
MaxQBiQ64674
PaxDbiQ64674
PRIDEiQ64674

2D gel databases

REPRODUCTION-2DPAGEQ64674

PTM databases

iPTMnetiQ64674
PhosphoSitePlusiQ64674
SwissPalmiQ64674

Expressioni

Gene expression databases

BgeeiENSMUSG00000006442
CleanExiMM_SRM
ExpressionAtlasiQ64674 baseline and differential
GenevisibleiQ64674 MM

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203498, 2 interactors
STRINGi10090.ENSMUSP00000006611

Structurei

3D structure databases

ProteinModelPortaliQ64674
SMRiQ64674
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 253PABSAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni155 – 156S-adenosylmethioninamine bindingBy similarity2
Regioni173 – 176Putrescine bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1562 Eukaryota
COG0421 LUCA
GeneTreeiENSGT00870000136521
HOGENOMiHOG000256147
HOVERGENiHBG000834
InParanoidiQ64674
KOiK00797
OMAiLWPGQSF
OrthoDBiEOG091G07LZ
PhylomeDBiQ64674
TreeFamiTF314466

Family and domain databases

Gene3Di2.30.140.10, 1 hit
HAMAPiMF_00198 Spermidine_synth, 1 hit
InterProiView protein in InterPro
IPR030374 PABS
IPR030373 PABS_CS
IPR029063 SAM-dependent_MTases
IPR001045 Spermi_synthase
IPR030668 Spermi_synthase_euk
IPR035246 Spermidine_synt_N
IPR037163 Spermidine_synt_N_sf
PfamiView protein in Pfam
PF17284 Spermine_synt_N, 1 hit
PIRSFiPIRSF000502 Spermidine_synth, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00417 speE, 1 hit
PROSITEiView protein in PROSITE
PS01330 PABS_1, 1 hit
PS51006 PABS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q64674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPGPDGPAA PGPAAIREGW FRETCSLWPG QALSLQVEQL LHHRRSRYQD
60 70 80 90 100
ILVFRSKTYG NVLVLDGVIQ CTERDEFSYQ EMIANLPLCS HPNPRKVLII
110 120 130 140 150
GGGDGGVLRE VVKHPSVESV VQCEIDEDVI EVSKKFLPGM AVGFSSSKLT
160 170 180 190 200
LHVGDGFEFM KQNQDAFDVI ITDSSDPMGP AESLFKESYY QLMKTALKED
210 220 230 240 250
GILCCQGECQ WLHLDLIKEM RHFCKSLFPV VDYAYCSIPT YPSGQIGFML
260 270 280 290 300
CSKNPSTNFR EPVQQLTQAQ VEQMQLKYYN SDMHRAAFVL PEFTRKALND

IS
Length:302
Mass (Da):33,995
Last modified:November 1, 1997 - v1
Checksum:i4C19B7AA3518054F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67748 Genomic DNA Translation: CAA91561.1
L19311 mRNA Translation: AAC37666.1
BC005566 mRNA Translation: AAH05566.1
CCDSiCCDS18940.1
RefSeqiNP_033298.1, NM_009272.4
UniGeneiMm.10

Genome annotation databases

EnsembliENSMUST00000006611; ENSMUSP00000006611; ENSMUSG00000006442
GeneIDi20810
KEGGimmu:20810
UCSCiuc008vuw.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSPEE_MOUSE
AccessioniPrimary (citable) accession number: Q64674
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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