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Protein

Spermidine synthase

Gene

Srm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine (By similarity).By similarity

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.

Enzyme regulationi

The activity is thought to be regulated mainly by the availability of decarboxylated S-adenosylmethionine.

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.
Proteins known to be involved in this subpathway in this organism are:
  1. Spermidine synthase (Srm)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49S-adenosylmethioninamineBy similarity1
Binding sitei79PutrescineBy similarity1
Binding sitei80S-adenosylmethioninamineBy similarity1
Binding sitei104S-adenosylmethioninamineBy similarity1
Binding sitei124S-adenosylmethioninamineBy similarity1
Active sitei173Proton acceptorBy similarity1
Binding sitei173S-adenosylmethioninamineBy similarity1
Binding sitei241PutrescineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-351202. Metabolism of polyamines.
UniPathwayiUPA00248; UER00314.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine synthase (EC:2.5.1.16)
Short name:
SPDSY
Alternative name(s):
Putrescine aminopropyltransferase
Gene namesi
Name:Srm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:102690. Srm.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564461 – 302Spermidine synthaseAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ64674.
MaxQBiQ64674.
PaxDbiQ64674.
PRIDEiQ64674.

2D gel databases

REPRODUCTION-2DPAGEQ64674.

PTM databases

iPTMnetiQ64674.
PhosphoSitePlusiQ64674.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006442.
CleanExiMM_SRM.
ExpressionAtlasiQ64674. baseline and differential.
GenevisibleiQ64674. MM.

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-1855000.
STRINGi10090.ENSMUSP00000006611.

Structurei

3D structure databases

ProteinModelPortaliQ64674.
SMRiQ64674.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 253PABSAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni155 – 156S-adenosylmethioninamine bindingBy similarity2
Regioni173 – 176Putrescine bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1562. Eukaryota.
COG0421. LUCA.
GeneTreeiENSGT00860000133776.
HOGENOMiHOG000256147.
HOVERGENiHBG000834.
InParanoidiQ64674.
KOiK00797.
OMAiLEAYCTT.
OrthoDBiEOG091G07LZ.
PhylomeDBiQ64674.
TreeFamiTF314466.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
IPR030668. Spermi_synthase_euk.
[Graphical view]
PIRSFiPIRSF000502. Spermidine_synth. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPGPDGPAA PGPAAIREGW FRETCSLWPG QALSLQVEQL LHHRRSRYQD
60 70 80 90 100
ILVFRSKTYG NVLVLDGVIQ CTERDEFSYQ EMIANLPLCS HPNPRKVLII
110 120 130 140 150
GGGDGGVLRE VVKHPSVESV VQCEIDEDVI EVSKKFLPGM AVGFSSSKLT
160 170 180 190 200
LHVGDGFEFM KQNQDAFDVI ITDSSDPMGP AESLFKESYY QLMKTALKED
210 220 230 240 250
GILCCQGECQ WLHLDLIKEM RHFCKSLFPV VDYAYCSIPT YPSGQIGFML
260 270 280 290 300
CSKNPSTNFR EPVQQLTQAQ VEQMQLKYYN SDMHRAAFVL PEFTRKALND

IS
Length:302
Mass (Da):33,995
Last modified:November 1, 1997 - v1
Checksum:i4C19B7AA3518054F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67748 Genomic DNA. Translation: CAA91561.1.
L19311 mRNA. Translation: AAC37666.1.
BC005566 mRNA. Translation: AAH05566.1.
CCDSiCCDS18940.1.
RefSeqiNP_033298.1. NM_009272.4.
UniGeneiMm.10.

Genome annotation databases

EnsembliENSMUST00000006611; ENSMUSP00000006611; ENSMUSG00000006442.
GeneIDi20810.
KEGGimmu:20810.
UCSCiuc008vuw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67748 Genomic DNA. Translation: CAA91561.1.
L19311 mRNA. Translation: AAC37666.1.
BC005566 mRNA. Translation: AAH05566.1.
CCDSiCCDS18940.1.
RefSeqiNP_033298.1. NM_009272.4.
UniGeneiMm.10.

3D structure databases

ProteinModelPortaliQ64674.
SMRiQ64674.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1855000.
STRINGi10090.ENSMUSP00000006611.

PTM databases

iPTMnetiQ64674.
PhosphoSitePlusiQ64674.

2D gel databases

REPRODUCTION-2DPAGEQ64674.

Proteomic databases

EPDiQ64674.
MaxQBiQ64674.
PaxDbiQ64674.
PRIDEiQ64674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006611; ENSMUSP00000006611; ENSMUSG00000006442.
GeneIDi20810.
KEGGimmu:20810.
UCSCiuc008vuw.1. mouse.

Organism-specific databases

CTDi6723.
MGIiMGI:102690. Srm.

Phylogenomic databases

eggNOGiKOG1562. Eukaryota.
COG0421. LUCA.
GeneTreeiENSGT00860000133776.
HOGENOMiHOG000256147.
HOVERGENiHBG000834.
InParanoidiQ64674.
KOiK00797.
OMAiLEAYCTT.
OrthoDBiEOG091G07LZ.
PhylomeDBiQ64674.
TreeFamiTF314466.

Enzyme and pathway databases

UniPathwayiUPA00248; UER00314.
ReactomeiR-MMU-351202. Metabolism of polyamines.

Miscellaneous databases

PROiQ64674.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006442.
CleanExiMM_SRM.
ExpressionAtlasiQ64674. baseline and differential.
GenevisibleiQ64674. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
IPR030668. Spermi_synthase_euk.
[Graphical view]
PIRSFiPIRSF000502. Spermidine_synth. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEE_MOUSE
AccessioniPrimary (citable) accession number: Q64674
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.