NAD(P)H dehydrogenase [quinone] 1 - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot Q64669 (NQO1_MOUSE)

Last modified June 16, 2009. Version 82. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    NAD(P)H dehydrogenase [quinone] 1
    EC=1.6.5.2
Alternative name(s):
    Quinone reductase 1
    NAD(P)H:quinone oxidoreductase 1
      Short name=QR1
    DT-diaphorase
      Short name=DTD
    Azoreductase
    Phylloquinone reductase
    Menadione reductase

Gene names

Name:	Nqo1
Synonyms:	Dia4, Nmo1, Nmor1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis By similarity.
Catalytic activity	NAD(P)H + a quinone = NAD(P)⁺ + a hydroquinone.
Cofactor	FAD.
Subunit structure	Homodimer. Ref.3
Subcellular location	Cytoplasm.
Induction	By polycyclic hydrocarbons such as dioxin (Governed by the aromatic hydrocarbon-responsive (AH) locus).
Sequence similarities	Belongs to the NAD(P)H dehydrogenase (quinone) family.

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Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of catalytic activity Inferred from direct assay. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)H dehydrogenase (quinone) activity Inferred from direct assay. Source: MGI coenzyme binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 274

273

NAD(P)H dehydrogenase [quinone] 1

PRO_0000071623

Regions

Nucleotide binding

18 – 19

FAD

Nucleotide binding

104 – 107

FAD

Nucleotide binding

148 – 151

FAD

Region

126 – 128

Substrate binding By similarity

Sites

Binding site

FAD

Binding site

FAD

Binding site

156

FAD

Binding site

201

FAD

Amino acid modifications

Modified residue

N-acetylalanine

Secondary structure

274

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q64669-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 68F380922FD72965
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FASTA

274

30,960

        10         20         30         40         50         60 
MAARRALIVL AHSEKTSFNY AMKEAAVEAL KKRGWEVLES DLYAMNFNPI ISRNDITGEL 

        70         80         90        100        110        120 
KDSKNFQYPS ESSLAYKEGR LSPDIVAEHK KLEAADLVIF QFPLQWFGVP AILKGWFERV 

       130        140        150        160        170        180 
LVAGFAYTYA AMYDNGPFQN KKTLLSITTG GSGSMYSLQG VHGDMNVILW PIQSGILRFC 

       190        200        210        220        230        240 
GFQVLEPQLV YSIGHTPPDA RMQILEGWKK RLETVWEETP LYFAPSSLFD LNFQAGFLMK 

       250        260        270 
KEVQEEQKKN KFGLSVGHHL GKSIPADNQI KARK

« Hide

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References

[1]	"Mouse dioxin-inducible NAD(P)H: menadione oxidoreductase: NMO1 cDNA sequence and genetic differences in mRNA levels." Vasiliou V., Theurer M.J., Puga A., Reuter S.F., Nebert D.W. Pharmacogenetics 4:341-348(1994) [PubMed: 7704040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
[2]	"Mouse liver NAD(P)H:quinone acceptor oxidoreductase: protein sequence analysis by tandem mass spectrometry, cDNA cloning, expression in Escherichia coli, and enzyme activity analysis." Chen S., Clarke P.E., Martino P.A., Deng P.S., Yeh C.H., Lee T.D., Prochaska H.J., Talalay P. Protein Sci. 3:1296-1304(1994) [PubMed: 7527260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[3]	"Structures of recombinant human and mouse NAD(P)H:quinone oxidoreductases: species comparison and structural changes with substrate binding and release." Faig M., Bianchet M.A., Talalay P., Chen S., Winski S., Ross D., Amzel L.M. Proc. Natl. Acad. Sci. U.S.A. 97:3177-3182(2000) [PubMed: 10706635] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH FAD, SUBUNIT.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U12961 mRNA. Translation: AAA80184.1.
S75951 mRNA. Translation: AAB32718.1.

IPI

IPI00263899.

PIR

A57691.

RefSeq

NP_032732.3.

UniGene

Mm.252

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DXQ	X-ray	2.80	A/B/C/D	2-274	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q64669.

Genome annotation databases

Ensembl

ENSMUSG00000003849. Mus musculus. [Contig view]

GeneID

18104.

KEGG

mmu:18104.

NMPDR

fig|10090.3.peg.19211.

Organism-specific databases

MGI

MGI:103187. Nqo1.

Phylogenomic databases

HOGENOM

Q64669.

HOVERGEN

Q64669.

OMA

Q64669. VERVYAY.

Enzyme and pathway databases

BRENDA

1.6.5.2. 244.

Gene expression databases

ArrayExpress

Q64669.

Bgee

Q64669.

CleanEx

MM_NQO1.

GermOnline

ENSMUSG00000003849. Mus musculus.

Family and domain databases

InterPro

IPR003680. Flavodoxin_fold.
[Graphical view]

Pfam

PF02525. Flavodoxin_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

293281.

SOURCE

Search...

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Entry information

Entry name

NQO1_MOUSE

Accession

Primary (citable) accession number: Q64669

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 82 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families