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Protein

NAD(P)H dehydrogenase [quinone] 1

Gene

Nqo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.By similarity

Catalytic activityi

NAD(P)H + a quinone = NAD(P)+ + a hydroquinone.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12FAD1 Publication1
Binding sitei67FAD1 Publication1
Binding sitei156FAD1 Publication1
Binding sitei201FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 19FAD1 Publication2
Nucleotide bindingi104 – 107FAD1 Publication4
Nucleotide bindingi148 – 151FAD1 Publication4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BRENDAi1.6.5.2. 3474.
ReactomeiR-MMU-350562. Regulation of ornithine decarboxylase (ODC).

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H dehydrogenase [quinone] 1 (EC:1.6.5.2)
Alternative name(s):
Azoreductase
DT-diaphorase
Short name:
DTD
Menadione reductase
NAD(P)H:quinone oxidoreductase 1
Phylloquinone reductase
Quinone reductase 1
Short name:
QR1
Gene namesi
Name:Nqo1
Synonyms:Dia4, Nmo1, Nmor1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:103187. Nqo1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5611.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000716232 – 274NAD(P)H dehydrogenase [quinone] 1Add BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei82PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ64669.
PaxDbiQ64669.
PRIDEiQ64669.

PTM databases

iPTMnetiQ64669.
PhosphoSitePlusiQ64669.

Expressioni

Inductioni

By polycyclic hydrocarbons such as dioxin (Governed by the aromatic hydrocarbon-responsive (AH) locus).

Gene expression databases

BgeeiENSMUSG00000003849.
CleanExiMM_NQO1.
ExpressionAtlasiQ64669. baseline and differential.
GenevisibleiQ64669. MM.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003947.

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi18 – 32Combined sources15
Beta strandi36 – 41Combined sources6
Helixi42 – 45Combined sources4
Helixi68 – 77Combined sources10
Helixi83 – 94Combined sources12
Beta strandi96 – 103Combined sources8
Helixi111 – 120Combined sources10
Turni123 – 125Combined sources3
Beta strandi129 – 131Combined sources3
Helixi133 – 135Combined sources3
Turni137 – 140Combined sources4
Beta strandi141 – 151Combined sources11
Helixi153 – 156Combined sources4
Beta strandi160 – 162Combined sources3
Helixi165 – 173Combined sources9
Turni174 – 176Combined sources3
Helixi177 – 180Combined sources4
Beta strandi188 – 191Combined sources4
Turni193 – 195Combined sources3
Helixi198 – 212Combined sources15
Helixi213 – 217Combined sources5
Helixi226 – 228Combined sources3
Turni233 – 236Combined sources4
Beta strandi237 – 239Combined sources3
Helixi241 – 247Combined sources7
Beta strandi254 – 256Combined sources3
Turni268 – 270Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DXQX-ray2.80A/B/C/D2-274[»]
ProteinModelPortaliQ64669.
SMRiQ64669.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ64669.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 128Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IKWF. Eukaryota.
COG2249. LUCA.
GeneTreeiENSGT00440000033410.
HOGENOMiHOG000149970.
HOVERGENiHBG029104.
InParanoidiQ64669.
KOiK00355.
OMAiYDKGPFQ.
OrthoDBiEOG091G183R.
PhylomeDBiQ64669.
TreeFamiTF300296.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR003680. Flavodoxin_fold.
IPR029039. Flavoprotein-like_dom.
[Graphical view]
PfamiPF02525. Flavodoxin_2. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARRALIVL AHSEKTSFNY AMKEAAVEAL KKRGWEVLES DLYAMNFNPI
60 70 80 90 100
ISRNDITGEL KDSKNFQYPS ESSLAYKEGR LSPDIVAEHK KLEAADLVIF
110 120 130 140 150
QFPLQWFGVP AILKGWFERV LVAGFAYTYA AMYDNGPFQN KKTLLSITTG
160 170 180 190 200
GSGSMYSLQG VHGDMNVILW PIQSGILRFC GFQVLEPQLV YSIGHTPPDA
210 220 230 240 250
RMQILEGWKK RLETVWEETP LYFAPSSLFD LNFQAGFLMK KEVQEEQKKN
260 270
KFGLSVGHHL GKSIPADNQI KARK
Length:274
Mass (Da):30,960
Last modified:January 23, 2007 - v3
Checksum:i68F380922FD72965
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12961 mRNA. Translation: AAA80184.1.
S75951 mRNA. Translation: AAB32718.1.
CCDSiCCDS40465.1.
PIRiA57691.
RefSeqiNP_032732.3. NM_008706.5.
UniGeneiMm.252.

Genome annotation databases

EnsembliENSMUST00000003947; ENSMUSP00000003947; ENSMUSG00000003849.
GeneIDi18104.
KEGGimmu:18104.
UCSCiuc009nhq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12961 mRNA. Translation: AAA80184.1.
S75951 mRNA. Translation: AAB32718.1.
CCDSiCCDS40465.1.
PIRiA57691.
RefSeqiNP_032732.3. NM_008706.5.
UniGeneiMm.252.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DXQX-ray2.80A/B/C/D2-274[»]
ProteinModelPortaliQ64669.
SMRiQ64669.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003947.

Chemistry databases

ChEMBLiCHEMBL5611.

PTM databases

iPTMnetiQ64669.
PhosphoSitePlusiQ64669.

Proteomic databases

MaxQBiQ64669.
PaxDbiQ64669.
PRIDEiQ64669.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003947; ENSMUSP00000003947; ENSMUSG00000003849.
GeneIDi18104.
KEGGimmu:18104.
UCSCiuc009nhq.1. mouse.

Organism-specific databases

CTDi1728.
MGIiMGI:103187. Nqo1.

Phylogenomic databases

eggNOGiENOG410IKWF. Eukaryota.
COG2249. LUCA.
GeneTreeiENSGT00440000033410.
HOGENOMiHOG000149970.
HOVERGENiHBG029104.
InParanoidiQ64669.
KOiK00355.
OMAiYDKGPFQ.
OrthoDBiEOG091G183R.
PhylomeDBiQ64669.
TreeFamiTF300296.

Enzyme and pathway databases

BRENDAi1.6.5.2. 3474.
ReactomeiR-MMU-350562. Regulation of ornithine decarboxylase (ODC).

Miscellaneous databases

EvolutionaryTraceiQ64669.
PROiQ64669.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003849.
CleanExiMM_NQO1.
ExpressionAtlasiQ64669. baseline and differential.
GenevisibleiQ64669. MM.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR003680. Flavodoxin_fold.
IPR029039. Flavoprotein-like_dom.
[Graphical view]
PfamiPF02525. Flavodoxin_2. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNQO1_MOUSE
AccessioniPrimary (citable) accession number: Q64669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.