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Protein

P2X purinoceptor 7

Gene

P2rx7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. channel activity Source: MGI
  3. copper ion binding Source: RGD
  4. extracellular ATP-gated cation channel activity Source: BHF-UCL
  5. lipopolysaccharide binding Source: Ensembl
  6. magnesium ion binding Source: RGD
  7. protein serine/threonine kinase activator activity Source: RGD
  8. purinergic nucleotide receptor activity Source: BHF-UCL
  9. receptor binding Source: BHF-UCL
  10. scaffold protein binding Source: RGD
  11. zinc ion binding Source: RGD

GO - Biological processi

  1. activation of MAPK activity Source: Ensembl
  2. apoptotic signaling pathway Source: BHF-UCL
  3. bleb assembly Source: BHF-UCL
  4. cell morphogenesis Source: Ensembl
  5. cell surface receptor signaling pathway Source: BHF-UCL
  6. cellular response to extracellular stimulus Source: Ensembl
  7. cell volume homeostasis Source: Ensembl
  8. ceramide biosynthetic process Source: Ensembl
  9. collagen metabolic process Source: Ensembl
  10. cytolysis Source: Ensembl
  11. defense response to Gram-positive bacterium Source: Ensembl
  12. extrinsic apoptotic signaling pathway Source: Ensembl
  13. gene expression Source: Ensembl
  14. homeostasis of number of cells within a tissue Source: Ensembl
  15. inflammatory response Source: Ensembl
  16. membrane budding Source: Ensembl
  17. membrane depolarization Source: BHF-UCL
  18. membrane protein ectodomain proteolysis Source: Ensembl
  19. mitochondrion organization Source: Ensembl
  20. multicellular organismal protein catabolic process Source: Ensembl
  21. NAD transport Source: Ensembl
  22. negative regulation of bone resorption Source: Ensembl
  23. negative regulation of MAPK cascade Source: Ensembl
  24. neuronal action potential Source: RGD
  25. organic cation transport Source: RGD
  26. phagolysosome assembly Source: Ensembl
  27. phospholipid transfer to membrane Source: Ensembl
  28. phospholipid translocation Source: Ensembl
  29. plasma membrane organization Source: Ensembl
  30. pore complex assembly Source: RGD
  31. positive regulation of bone mineralization Source: Ensembl
  32. positive regulation of calcium ion transport into cytosol Source: Ensembl
  33. positive regulation of cytolysis Source: BHF-UCL
  34. positive regulation of cytoskeleton organization Source: BHF-UCL
  35. positive regulation of gamma-aminobutyric acid secretion Source: Ensembl
  36. positive regulation of glutamate secretion Source: Ensembl
  37. positive regulation of interleukin-1 alpha secretion Source: Ensembl
  38. positive regulation of interleukin-1 beta secretion Source: RGD
  39. positive regulation of interleukin-6 production Source: Ensembl
  40. positive regulation of lymphocyte apoptotic process Source: Ensembl
  41. positive regulation of mitochondrial depolarization Source: Ensembl
  42. positive regulation of prostaglandin secretion Source: Ensembl
  43. positive regulation of protein phosphorylation Source: RGD
  44. positive regulation of T cell mediated cytotoxicity Source: Ensembl
  45. protein oligomerization Source: Ensembl
  46. protein phosphorylation Source: Ensembl
  47. protein processing Source: Ensembl
  48. purinergic nucleotide receptor signaling pathway Source: GOC
  49. reactive oxygen species metabolic process Source: Ensembl
  50. regulation of sodium ion transport Source: BHF-UCL
  51. release of sequestered calcium ion into cytosol Source: Ensembl
  52. response to ATP Source: BHF-UCL
  53. response to calcium ion Source: Ensembl
  54. response to drug Source: Ensembl
  55. response to electrical stimulus Source: Ensembl
  56. response to fluid shear stress Source: Ensembl
  57. response to lipopolysaccharide Source: Ensembl
  58. response to mechanical stimulus Source: Ensembl
  59. response to zinc ion Source: Ensembl
  60. sensory perception of pain Source: Ensembl
  61. skeletal system morphogenesis Source: Ensembl
  62. synaptic vesicle exocytosis Source: Ensembl
  63. T cell homeostasis Source: Ensembl
  64. T cell proliferation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_284271. The NLRP3 inflammasome.

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 7
Short name:
P2X7
Alternative name(s):
ATP receptor
P2Z receptor
Purinergic receptor
Gene namesi
Name:P2rx7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi3241. P2rx7.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei26 – 4621Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini47 – 334288ExtracellularSequence AnalysisAdd
BLAST
Transmembranei335 – 35521Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini356 – 595240CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. bleb Source: BHF-UCL
  2. cell-cell junction Source: Ensembl
  3. cytoplasm Source: BHF-UCL
  4. external side of plasma membrane Source: Ensembl
  5. integral component of nuclear inner membrane Source: RGD
  6. integral component of plasma membrane Source: Ensembl
  7. membrane raft Source: RGD
  8. neuromuscular junction Source: Ensembl
  9. neuronal cell body Source: Ensembl
  10. neuron projection Source: RGD
  11. plasma membrane Source: BHF-UCL
  12. protein complex Source: RGD
  13. terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595P2X purinoceptor 7PRO_0000161562Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi119 ↔ 168By similarity
Modified residuei125 – 1251ADP-ribosylarginineBy similarity
Disulfide bondi129 ↔ 152By similarity
Disulfide bondi135 ↔ 162By similarity
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi216 ↔ 226By similarity
Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi260 ↔ 269By similarity
Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Phosphorylation results in its inactivation.By similarity
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.By similarity
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.By similarity

Keywords - PTMi

ADP-ribosylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ64663.
PRIDEiQ64663.

PTM databases

PhosphoSiteiQ64663.

Expressioni

Gene expression databases

GenevestigatoriQ64663.

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB (By similarity). Interacts (via C-terminus) with EMP2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248286. 11 interactions.
IntActiQ64663. 2 interactions.
MINTiMINT-3379474.

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG38999.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiQ64663.
KOiK05220.
OMAiLCPEYPT.
OrthoDBiEOG78PV92.
PhylomeDBiQ64663.
TreeFamiTF328633.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003050. P2X7_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF13. PTHR10125:SF13. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PRINTSiPR01314. P2X7RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPACCSWNDV FQYETNKVTR IQSVNYGTIK WILHMTVFSY VSFALMSDKL
60 70 80 90 100
YQRKEPLISS VHTKVKGVAE VTENVTEGGV TKLVHGIFDT ADYTLPLQGN
110 120 130 140 150
SFFVMTNYLK SEGQEQKLCP EYPSRGKQCH SDQGCIKGWM DPQSKGIQTG
160 170 180 190 200
RCIPYDQKRK TCEIFAWCPA EEGKEAPRPA LLRSAENFTV LIKNNIDFPG
210 220 230 240 250
HNYTTRNILP GMNISCTFHK TWNPQCPIFR LGDIFQEIGE NFTEVAVQGG
260 270 280 290 300
IMGIEIYWDC NLDSWSHRCQ PKYSFRRLDD KYTNESLFPG YNFRYAKYYK
310 320 330 340 350
ENGMEKRTLI KAFGVRFDIL VFGTGGKFDI IQLVVYIGST LSYFGLATVC
360 370 380 390 400
IDLIINTYAS TCCRSRVYPS CKCCEPCAVN EYYYRKKCEP IVEPKPTLKY
410 420 430 440 450
VSFVDEPHIW MVDQQLLGKS LQDVKGQEVP RPQTDFLELS RLSLSLHHSP
460 470 480 490 500
PIPGQPEEMQ LLQIEAVPRS RDSPDWCQCG NCLPSQLPEN RRALEELCCR
510 520 530 540 550
RKPGQCITTS ELFSKIVLSR EALQLLLLYQ EPLLALEGEA INSKLRHCAY
560 570 580 590
RSYATWRFVS QDMADFAILP SCCRWKIRKE FPKTQGQYSG FKYPY
Length:595
Mass (Da):68,392
Last modified:October 31, 1996 - v1
Checksum:i4A6DD6058E5988D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95882 mRNA. Translation: CAA65131.1.
RefSeqiNP_062129.1. NM_019256.1.
UniGeneiRn.10510.

Genome annotation databases

EnsembliENSRNOT00000001746; ENSRNOP00000001746; ENSRNOG00000001296.
GeneIDi29665.
KEGGirno:29665.
UCSCiRGD:3241. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95882 mRNA. Translation: CAA65131.1.
RefSeqiNP_062129.1. NM_019256.1.
UniGeneiRn.10510.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248286. 11 interactions.
IntActiQ64663. 2 interactions.
MINTiMINT-3379474.

Chemistry

BindingDBiQ64663.
ChEMBLiCHEMBL2496.
GuidetoPHARMACOLOGYi484.

PTM databases

PhosphoSiteiQ64663.

Proteomic databases

PaxDbiQ64663.
PRIDEiQ64663.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001746; ENSRNOP00000001746; ENSRNOG00000001296.
GeneIDi29665.
KEGGirno:29665.
UCSCiRGD:3241. rat.

Organism-specific databases

CTDi5027.
RGDi3241. P2rx7.

Phylogenomic databases

eggNOGiNOG38999.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiQ64663.
KOiK05220.
OMAiLCPEYPT.
OrthoDBiEOG78PV92.
PhylomeDBiQ64663.
TreeFamiTF328633.

Enzyme and pathway databases

ReactomeiREACT_284271. The NLRP3 inflammasome.

Miscellaneous databases

NextBioi609971.
PROiQ64663.

Gene expression databases

GenevestigatoriQ64663.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003050. P2X7_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF13. PTHR10125:SF13. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PRINTSiPR01314. P2X7RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cytolytic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7)."
    Surprenant A., Rassendren F., Kawashima E., North R.A., Buell G.N.
    Science 272:735-738(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: M33.

Entry informationi

Entry nameiP2RX7_RAT
AccessioniPrimary (citable) accession number: Q64663
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.