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Q64663

- P2RX7_RAT

UniProt

Q64663 - P2RX7_RAT

Protein

P2X purinoceptor 7

Gene

P2rx7

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. channel activity Source: MGI
    3. copper ion binding Source: RGD
    4. extracellular ATP-gated cation channel activity Source: BHF-UCL
    5. lipopolysaccharide binding Source: Ensembl
    6. magnesium ion binding Source: RGD
    7. protein binding Source: UniProtKB
    8. protein serine/threonine kinase activator activity Source: RGD
    9. purinergic nucleotide receptor activity Source: BHF-UCL
    10. receptor binding Source: BHF-UCL
    11. scaffold protein binding Source: RGD
    12. zinc ion binding Source: RGD

    GO - Biological processi

    1. activation of MAPK activity Source: Ensembl
    2. apoptotic signaling pathway Source: BHF-UCL
    3. bleb assembly Source: BHF-UCL
    4. cell morphogenesis Source: Ensembl
    5. cell surface receptor signaling pathway Source: BHF-UCL
    6. cellular response to extracellular stimulus Source: Ensembl
    7. cell volume homeostasis Source: Ensembl
    8. ceramide biosynthetic process Source: Ensembl
    9. collagen metabolic process Source: Ensembl
    10. cytolysis Source: Ensembl
    11. defense response to Gram-positive bacterium Source: Ensembl
    12. homeostasis of number of cells within a tissue Source: Ensembl
    13. inflammatory response Source: Ensembl
    14. membrane budding Source: Ensembl
    15. membrane depolarization Source: BHF-UCL
    16. membrane protein ectodomain proteolysis Source: Ensembl
    17. mitochondrion organization Source: Ensembl
    18. multicellular organismal protein catabolic process Source: Ensembl
    19. NAD transport Source: Ensembl
    20. negative regulation of bone resorption Source: Ensembl
    21. negative regulation of MAPK cascade Source: Ensembl
    22. neuronal action potential Source: RGD
    23. organic cation transport Source: RGD
    24. phagolysosome assembly Source: Ensembl
    25. phospholipid transfer to membrane Source: Ensembl
    26. phospholipid translocation Source: Ensembl
    27. plasma membrane organization Source: Ensembl
    28. pore complex assembly Source: RGD
    29. positive regulation of bone mineralization Source: Ensembl
    30. positive regulation of cytolysis Source: BHF-UCL
    31. positive regulation of cytoskeleton organization Source: BHF-UCL
    32. positive regulation of gamma-aminobutyric acid secretion Source: Ensembl
    33. positive regulation of glutamate secretion Source: Ensembl
    34. positive regulation of interleukin-1 alpha secretion Source: Ensembl
    35. positive regulation of interleukin-1 beta secretion Source: RGD
    36. positive regulation of interleukin-6 production Source: Ensembl
    37. positive regulation of lymphocyte apoptotic process Source: Ensembl
    38. positive regulation of mitochondrial depolarization Source: Ensembl
    39. positive regulation of prostaglandin secretion Source: Ensembl
    40. positive regulation of protein phosphorylation Source: RGD
    41. positive regulation of T cell mediated cytotoxicity Source: Ensembl
    42. protein oligomerization Source: Ensembl
    43. protein phosphorylation Source: Ensembl
    44. protein processing Source: Ensembl
    45. purinergic nucleotide receptor signaling pathway Source: GOC
    46. reactive oxygen species metabolic process Source: Ensembl
    47. regulation of sodium ion transport Source: BHF-UCL
    48. release of sequestered calcium ion into cytosol Source: Ensembl
    49. response to ATP Source: BHF-UCL
    50. response to calcium ion Source: Ensembl
    51. response to drug Source: Ensembl
    52. response to electrical stimulus Source: Ensembl
    53. response to fluid shear stress Source: Ensembl
    54. response to lipopolysaccharide Source: Ensembl
    55. response to mechanical stimulus Source: Ensembl
    56. response to zinc ion Source: Ensembl
    57. sensory perception of pain Source: Ensembl
    58. skeletal system morphogenesis Source: Ensembl
    59. synaptic vesicle exocytosis Source: Ensembl
    60. T cell homeostasis Source: Ensembl
    61. T cell proliferation Source: Ensembl
    62. transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196450. The NLRP3 inflammasome.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    P2X purinoceptor 7
    Short name:
    P2X7
    Alternative name(s):
    ATP receptor
    P2Z receptor
    Purinergic receptor
    Gene namesi
    Name:P2rx7
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 12

    Organism-specific databases

    RGDi3241. P2rx7.

    Subcellular locationi

    GO - Cellular componenti

    1. bleb Source: BHF-UCL
    2. cell-cell junction Source: Ensembl
    3. cytoplasm Source: BHF-UCL
    4. external side of plasma membrane Source: Ensembl
    5. integral component of nuclear inner membrane Source: RGD
    6. integral component of plasma membrane Source: Ensembl
    7. membrane raft Source: RGD
    8. neuromuscular junction Source: Ensembl
    9. neuronal cell body Source: Ensembl
    10. neuron projection Source: RGD
    11. plasma membrane Source: BHF-UCL
    12. protein complex Source: RGD
    13. terminal bouton Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 595595P2X purinoceptor 7PRO_0000161562Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi119 ↔ 168By similarity
    Modified residuei125 – 1251ADP-ribosylarginineBy similarity
    Disulfide bondi129 ↔ 152By similarity
    Disulfide bondi135 ↔ 162By similarity
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi216 ↔ 226By similarity
    Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi260 ↔ 269By similarity
    Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylation results in its inactivation.By similarity
    ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.By similarity
    Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.By similarity

    Keywords - PTMi

    ADP-ribosylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ64663.
    PRIDEiQ64663.

    PTM databases

    PhosphoSiteiQ64663.

    Expressioni

    Gene expression databases

    GenevestigatoriQ64663.

    Interactioni

    Subunit structurei

    Functional P2XRs are organized as homomeric and heteromeric trimers. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB By similarity.By similarity

    Protein-protein interaction databases

    BioGridi248286. 11 interactions.
    IntActiQ64663. 2 interactions.
    MINTiMINT-3379474.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2525CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini47 – 334288ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini356 – 595240CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4621Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei335 – 35521Helical; Name=2Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the P2X receptor family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG38999.
    GeneTreeiENSGT00390000016028.
    HOGENOMiHOG000232042.
    HOVERGENiHBG053086.
    InParanoidiQ64663.
    KOiK05220.
    OMAiLCPEYPT.
    OrthoDBiEOG78PV92.
    PhylomeDBiQ64663.
    TreeFamiTF328633.

    Family and domain databases

    Gene3Di2.60.490.10. 1 hit.
    InterProiIPR003050. P2X7_purnocptor.
    IPR027309. P2X_extracellular_dom.
    IPR001429. P2X_purnocptor.
    [Graphical view]
    PANTHERiPTHR10125. PTHR10125. 1 hit.
    PTHR10125:SF13. PTHR10125:SF13. 1 hit.
    PfamiPF00864. P2X_receptor. 1 hit.
    [Graphical view]
    PRINTSiPR01314. P2X7RECEPTOR.
    PR01307. P2XRECEPTOR.
    TIGRFAMsiTIGR00863. P2X. 1 hit.
    PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64663-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPACCSWNDV FQYETNKVTR IQSVNYGTIK WILHMTVFSY VSFALMSDKL    50
    YQRKEPLISS VHTKVKGVAE VTENVTEGGV TKLVHGIFDT ADYTLPLQGN 100
    SFFVMTNYLK SEGQEQKLCP EYPSRGKQCH SDQGCIKGWM DPQSKGIQTG 150
    RCIPYDQKRK TCEIFAWCPA EEGKEAPRPA LLRSAENFTV LIKNNIDFPG 200
    HNYTTRNILP GMNISCTFHK TWNPQCPIFR LGDIFQEIGE NFTEVAVQGG 250
    IMGIEIYWDC NLDSWSHRCQ PKYSFRRLDD KYTNESLFPG YNFRYAKYYK 300
    ENGMEKRTLI KAFGVRFDIL VFGTGGKFDI IQLVVYIGST LSYFGLATVC 350
    IDLIINTYAS TCCRSRVYPS CKCCEPCAVN EYYYRKKCEP IVEPKPTLKY 400
    VSFVDEPHIW MVDQQLLGKS LQDVKGQEVP RPQTDFLELS RLSLSLHHSP 450
    PIPGQPEEMQ LLQIEAVPRS RDSPDWCQCG NCLPSQLPEN RRALEELCCR 500
    RKPGQCITTS ELFSKIVLSR EALQLLLLYQ EPLLALEGEA INSKLRHCAY 550
    RSYATWRFVS QDMADFAILP SCCRWKIRKE FPKTQGQYSG FKYPY 595
    Length:595
    Mass (Da):68,392
    Last modified:November 1, 1996 - v1
    Checksum:i4A6DD6058E5988D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95882 mRNA. Translation: CAA65131.1.
    RefSeqiNP_062129.1. NM_019256.1.
    UniGeneiRn.10510.

    Genome annotation databases

    EnsembliENSRNOT00000001746; ENSRNOP00000001746; ENSRNOG00000001296.
    GeneIDi29665.
    KEGGirno:29665.
    UCSCiRGD:3241. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95882 mRNA. Translation: CAA65131.1 .
    RefSeqi NP_062129.1. NM_019256.1.
    UniGenei Rn.10510.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248286. 11 interactions.
    IntActi Q64663. 2 interactions.
    MINTi MINT-3379474.

    Chemistry

    BindingDBi Q64663.
    ChEMBLi CHEMBL2496.
    GuidetoPHARMACOLOGYi 484.

    PTM databases

    PhosphoSitei Q64663.

    Proteomic databases

    PaxDbi Q64663.
    PRIDEi Q64663.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001746 ; ENSRNOP00000001746 ; ENSRNOG00000001296 .
    GeneIDi 29665.
    KEGGi rno:29665.
    UCSCi RGD:3241. rat.

    Organism-specific databases

    CTDi 5027.
    RGDi 3241. P2rx7.

    Phylogenomic databases

    eggNOGi NOG38999.
    GeneTreei ENSGT00390000016028.
    HOGENOMi HOG000232042.
    HOVERGENi HBG053086.
    InParanoidi Q64663.
    KOi K05220.
    OMAi LCPEYPT.
    OrthoDBi EOG78PV92.
    PhylomeDBi Q64663.
    TreeFami TF328633.

    Enzyme and pathway databases

    Reactomei REACT_196450. The NLRP3 inflammasome.

    Miscellaneous databases

    NextBioi 609971.
    PROi Q64663.

    Gene expression databases

    Genevestigatori Q64663.

    Family and domain databases

    Gene3Di 2.60.490.10. 1 hit.
    InterProi IPR003050. P2X7_purnocptor.
    IPR027309. P2X_extracellular_dom.
    IPR001429. P2X_purnocptor.
    [Graphical view ]
    PANTHERi PTHR10125. PTHR10125. 1 hit.
    PTHR10125:SF13. PTHR10125:SF13. 1 hit.
    Pfami PF00864. P2X_receptor. 1 hit.
    [Graphical view ]
    PRINTSi PR01314. P2X7RECEPTOR.
    PR01307. P2XRECEPTOR.
    TIGRFAMsi TIGR00863. P2X. 1 hit.
    PROSITEi PS01212. P2X_RECEPTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cytolytic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7)."
      Surprenant A., Rassendren F., Kawashima E., North R.A., Buell G.N.
      Science 272:735-738(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: M33.

    Entry informationi

    Entry nameiP2RX7_RAT
    AccessioniPrimary (citable) accession number: Q64663
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3