Reviewed, UniProtKB/Swiss-Prot Q64658 (C11B2_MESAU)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 11B2, mitochondrial
    EC=1.14.15.4
    EC=1.14.15.5
Alternative name(s):
    C450XIB2
    P-450Aldo
    Aldosterone synthase
      Short name=ALDOS
    Aldosterone-synthesizing enzyme
    Steroid 18-hydroxylase
    P-450C18

Gene names

Name:

CYP11B2

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone By similarity.
Catalytic activity	A steroid + reduced adrenal ferredoxin + O(2) = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H(2)O. Corticosterone + reduced adrenal ferredoxin + O(2) = 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H(2)O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane.
Tissue specificity	Adrenal gland.
Induction	By low sodium intake.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	corticosterone 18-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 11-beta-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion By similarity
	Chain	25 – 500	476	Cytochrome P450 11B2, mitochondrial		PRO_0000003599
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64658-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 38251EDAD2085DE8	FASTA	500	57,332
	10 20 30 40 50 60 MALRAKADVW LARPWQCLPR TRALGTTAAL APNTLRPFEA IPQYSRNRWL KMLQILREEG 70 80 90 100 110 120 QEGLHLEMHE AFRELGPIFR YSMGRTQVVS VMLPEDAEKL HQVESMHPRR MHLEPWVAHR 130 140 150 160 170 180 EHRGLSRGVF LLNGPEWRFN RLRLNPHVLS PKAVQKFVPM VDMVARDFLE SLKKKVFQNA 190 200 210 220 230 240 RGSLTMDVQQ SLFNYSIEAS NFVLFGERLG LLGHDLSPAS LTFIHALHSV FKTTPQLMFL 250 260 270 280 290 300 PRSLTRWTST RVWKEHFEAW DVISEYVNRC IRKVHQELRL GSPHTYSGIV AELMSQGALP 310 320 330 340 350 360 LDAIRANSIE LTAGSVDTTT FPLVMALFEL ARNPDVQQAV RQESLAAEAS VAANPQRAMS 370 380 390 400 410 420 DLPLLRAVLK ETLRLYPVGG FLERILSSDL VLQNYHVPAG TLVLLYLYSM GRNPAVFPRP 430 440 450 460 470 480 EHYLPQRWLE RNGSFQHLTF GFGVRQCLGK RLAQVEMLLL LHHVLKSFRV ETQEREDVRM 490 500 VYRFVLAPSS SPLLTFRPVS

References

[1]	"The presence of two cytochrome P450 aldosterone synthase mRNAs in the hamster adrenal." Lehoux J.-G., Mason J.I., Bernard H., Ducharme L., Lehoux J., Veronneau S., Lefebvre A. J. Steroid Biochem. Mol. Biol. 49:131-137(1994) [PubMed: 8031709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal glomerulosa.
[2]	"Characterization of the hamster CYP11B2 gene encoding adrenal cytochrome P450 aldosterone synthase." Coulombe N., Lefebvre A., Lehoux J.-G. DNA Cell Biol. 16:993-1002(1997) [PubMed: 9303441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	S73810 mRNA. Translation: AAB31349.1. U71280 Genomic DNA. Translation: AAB16805.1.
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q64658.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002399. Cyt_P450_mit. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00408. MITP450. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C11B2_MESAU

Accession

Primary (citable) accession number: Q64658

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)