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Q64654 (CP51A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Lanosterol 14-alpha demethylase
Short name=LDM
EC=1.14.13.70
Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name=Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase
Gene names
Name:Cyp51a1
Synonyms:Cyp51
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Catalytic activity

Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Pathway

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.

Subcellular location

Endoplasmic reticulum membrane Potential. Microsome membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Lanosterol 14-alpha demethylase
PRO_0000052001

Regions

Transmembrane24 – 4421Helical; Potential

Sites

Metal binding4491Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict1811E → K in AAA87074. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q64654 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 33D8F345FFE9CF21

FASTA50356,707
        10         20         30         40         50         60 
MVLLGLLQSG GSVLGQAMEQ VTGGNLLSTL LIACAFTLSL VYLFRLAVGH MVQLPAGAKS 

        70         80         90        100        110        120 
PPYIYSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM VGKTFTYLLG SDAAALLFNS 

       130        140        150        160        170        180 
KNEDLNAEEV YGRLTTPVFG KGVAYDVPNA VFLEQKKILK SGLNIAHFKQ YVSIIEKEAK 

       190        200        210        220        230        240 
EYFKSWGESG ERNVFEALSE LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL 

       250        260        270        280        290        300 
LPGWLPLPSF RRRDRAHREI KNIFYKAIQK RRLSKEPAED ILQTLLDSTY KDGRPLTDDE 

       310        320        330        340        350        360 
IAGMLIGLLL AGQHTSSTTS AWMGFFLARD KPLQDKCYLE QKTVCGEDLP PLTYEQLKDL 

       370        380        390        400        410        420 
NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ VCVSPTVNQR LKDSWVERLD 

       430        440        450        460        470        480 
FNPDRYLQDN PASGEKFAYV PFGAGRHRCI GENFAYVQIK TIWSTMLRLY EFDLINGYFP 

       490        500 
SVNYTTMIHT PENPVIRYKR RSK

« Hide

References

« Hide 'large scale' references
[1]"Occurrence of a P450 showing high homology to yeast lanosterol 14-demethylase (P450(14DM)) in the rat liver."
Aoyama Y., Funae Y., Noshiro M., Horiuchi T., Yoshida Y.
Biochem. Biophys. Res. Commun. 201:1320-1326(1994) [PubMed: 8024575] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Structural and evolutionary studies on sterol 14-demethylase P450 (CYP51), the most conserved P450 monooxygenase: I. Structural analyses of the gene and multiple sizes of mRNA."
Noshiro M., Aoyama Y., Kawamoto T., Gotoh O., Horiuchi T., Yoshida Y.
J. Biochem. 122:1114-1121(1997) [PubMed: 9498553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Wistar.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[4]"Cloning and functional expression of the cDNA encoding rat lanosterol 14-alpha demethylase."
Sloane D.L., So O.Y., Leung R., Scarafia L.E., Saldou N., Jarnagin K., Swinney D.C.
Gene 161:243-248(1995) [PubMed: 7665087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-503.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D55681 mRNA. Translation: BAA09529.1.
AB004096 Genomic DNA. Translation: BAA20354.1.
BC087033 mRNA. Translation: AAH87033.1.
U17697 mRNA. Translation: AAA87074.1.
IPIIPI00214482.
PIRJC4240.
JC4758.
RefSeqNP_037073.1. NM_012941.2.
UniGeneRn.107152.

3D structure databases

ProteinModelPortalQ64654.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ64654.

Proteomic databases

PRIDEQ64654.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000009985; ENSRNOP00000009985; ENSRNOG00000007234.
GeneID25427.
KEGGrno:25427.
UCSCNM_012941. rat.

Organism-specific databases

CTD13121.
RGD2481. Cyp51.

Phylogenomic databases

eggNOGroNOG14720.
GeneTreeENSGT00440000033325.
HOVERGENHBG051102.
InParanoidQ64654.
OMAATYKDGR.
OrthoDBEOG4M0F1P.
PhylomeDBQ64654.

Gene expression databases

ArrayExpressQ64654.
GenevestigatorQ64654.
GermOnlineENSRNOG00000007234. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
KOK05917.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
SUPFAMSSF48264. Cytochrome_P450. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio606607.

Entry information

Entry nameCP51A_RAT
AccessionPrimary (citable) accession number: Q64654
Secondary accession number(s): Q64549
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 16, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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