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Protein

Lanosterol 14-alpha demethylase

Gene

Cyp51a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

hemeBy similarity

Pathwayi: zymosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi449 – 4491Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • sterol 14-demethylase activity Source: RGD

GO - Biological processi

  • cholesterol biosynthetic process via 24,25-dihydrolanosterol Source: RGD
  • demethylation Source: GOC
  • regulation of cholesterol biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.70. 5301.
ReactomeiR-RNO-191273. Cholesterol biosynthesis.
R-RNO-211976. Endogenous sterols.
R-RNO-2426168. Activation of gene expression by SREBF (SREBP).
UniPathwayiUPA00770; UER00754.

Chemistry

SwissLipidsiSLP:000001302.

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol 14-alpha demethylase (EC:1.14.13.70)
Short name:
LDM
Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name:
Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase
Gene namesi
Name:Cyp51a1
Synonyms:Cyp51
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2481. Cyp51.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4981.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Lanosterol 14-alpha demethylasePRO_0000052001Add
BLAST

Proteomic databases

PaxDbiQ64654.
PRIDEiQ64654.

Expressioni

Gene expression databases

GenevisibleiQ64654. RN.

Interactioni

Protein-protein interaction databases

BioGridi247462. 1 interaction.
STRINGi10116.ENSRNOP00000009985.

Chemistry

BindingDBiQ64654.

Structurei

3D structure databases

ProteinModelPortaliQ64654.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00800000124060.
HOGENOMiHOG000042780.
HOVERGENiHBG051102.
InParanoidiQ64654.
KOiK05917.
OMAiMIHTPHN.
OrthoDBiEOG7PZRX3.
PhylomeDBiQ64654.
TreeFamiTF105091.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLGLLQSG GSVLGQAMEQ VTGGNLLSTL LIACAFTLSL VYLFRLAVGH
60 70 80 90 100
MVQLPAGAKS PPYIYSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM
110 120 130 140 150
VGKTFTYLLG SDAAALLFNS KNEDLNAEEV YGRLTTPVFG KGVAYDVPNA
160 170 180 190 200
VFLEQKKILK SGLNIAHFKQ YVSIIEKEAK EYFKSWGESG ERNVFEALSE
210 220 230 240 250
LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL LPGWLPLPSF
260 270 280 290 300
RRRDRAHREI KNIFYKAIQK RRLSKEPAED ILQTLLDSTY KDGRPLTDDE
310 320 330 340 350
IAGMLIGLLL AGQHTSSTTS AWMGFFLARD KPLQDKCYLE QKTVCGEDLP
360 370 380 390 400
PLTYEQLKDL NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ
410 420 430 440 450
VCVSPTVNQR LKDSWVERLD FNPDRYLQDN PASGEKFAYV PFGAGRHRCI
460 470 480 490 500
GENFAYVQIK TIWSTMLRLY EFDLINGYFP SVNYTTMIHT PENPVIRYKR

RSK
Length:503
Mass (Da):56,707
Last modified:November 1, 1996 - v1
Checksum:i33D8F345FFE9CF21
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811E → K in AAA87074 (PubMed:7665087).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D55681 mRNA. Translation: BAA09529.1.
AB004096 Genomic DNA. Translation: BAA20354.1.
BC087033 mRNA. Translation: AAH87033.1.
U17697 mRNA. Translation: AAA87074.1.
PIRiJC4240.
JC4758.
RefSeqiNP_037073.1. NM_012941.2.
UniGeneiRn.107152.

Genome annotation databases

EnsembliENSRNOT00000009985; ENSRNOP00000009985; ENSRNOG00000007234.
GeneIDi25427.
KEGGirno:25427.
UCSCiRGD:2481. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D55681 mRNA. Translation: BAA09529.1.
AB004096 Genomic DNA. Translation: BAA20354.1.
BC087033 mRNA. Translation: AAH87033.1.
U17697 mRNA. Translation: AAA87074.1.
PIRiJC4240.
JC4758.
RefSeqiNP_037073.1. NM_012941.2.
UniGeneiRn.107152.

3D structure databases

ProteinModelPortaliQ64654.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247462. 1 interaction.
STRINGi10116.ENSRNOP00000009985.

Chemistry

BindingDBiQ64654.
ChEMBLiCHEMBL4981.
SwissLipidsiSLP:000001302.

Proteomic databases

PaxDbiQ64654.
PRIDEiQ64654.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009985; ENSRNOP00000009985; ENSRNOG00000007234.
GeneIDi25427.
KEGGirno:25427.
UCSCiRGD:2481. rat.

Organism-specific databases

CTDi13121.
RGDi2481. Cyp51.

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00800000124060.
HOGENOMiHOG000042780.
HOVERGENiHBG051102.
InParanoidiQ64654.
KOiK05917.
OMAiMIHTPHN.
OrthoDBiEOG7PZRX3.
PhylomeDBiQ64654.
TreeFamiTF105091.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754.
BRENDAi1.14.13.70. 5301.
ReactomeiR-RNO-191273. Cholesterol biosynthesis.
R-RNO-211976. Endogenous sterols.
R-RNO-2426168. Activation of gene expression by SREBF (SREBP).

Miscellaneous databases

NextBioi606607.
PROiQ64654.

Gene expression databases

GenevisibleiQ64654. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Occurrence of a P450 showing high homology to yeast lanosterol 14-demethylase (P450(14DM)) in the rat liver."
    Aoyama Y., Funae Y., Noshiro M., Horiuchi T., Yoshida Y.
    Biochem. Biophys. Res. Commun. 201:1320-1326(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Structural and evolutionary studies on sterol 14-demethylase P450 (CYP51), the most conserved P450 monooxygenase: I. Structural analyses of the gene and multiple sizes of mRNA."
    Noshiro M., Aoyama Y., Kawamoto T., Gotoh O., Horiuchi T., Yoshida Y.
    J. Biochem. 122:1114-1121(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Wistar.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Cloning and functional expression of the cDNA encoding rat lanosterol 14-alpha demethylase."
    Sloane D.L., So O.Y., Leung R., Scarafia L.E., Saldou N., Jarnagin K., Swinney D.C.
    Gene 161:243-248(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-503.
    Tissue: Liver.

Entry informationi

Entry nameiCP51A_RAT
AccessioniPrimary (citable) accession number: Q64654
Secondary accession number(s): Q64549
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.