Reviewed, UniProtKB/Swiss-Prot Q64640 (ADK_RAT)

Names and origin

Protein names

Recommended name:
    Adenosine kinase
      Short name=AK
    EC=2.7.1.20
Alternative name(s):
    Adenosine 5'-phosphotransferase

Gene names

Name:

Adk

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Catalytic activity	ATP + adenosine = ADP + AMP.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Purine salvage
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	adenosine metabolic process Inferred from direct assay. Source: RGD purine ribonucleoside salvage Ref.3 Non-traceable author statement. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosine kinase activity Inferred from direct assay. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 361

361

Adenosine kinase

PRO_0000080055

Sites

Active site

316

1

By similarity

Metal binding

48

1

Magnesium 1 By similarity

Metal binding

147

1

Magnesium 2 By similarity

Experimental info

Sequence conflict

121

1

D → N in AAB03110. Ref.3

Sequence conflict

144

1

T → S in AAB03110. Ref.3

Sequence conflict

155

1

A → R in AAB03110. Ref.3

Sequence conflict

198

1

V → M in AAB03110. Ref.3

Sequence conflict

217 – 218

2

FI → LL in AAB03110. Ref.3

Sequence conflict

228

1

E → A in AAB03110. Ref.3

Sequence conflict

359

1

D → N in AAH81712. Ref.2

Secondary structure

1

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361

Helix Strand Turn

Details...

Beta strand

25 – 28

4

Beta strand

32 – 38

7

Helix

41 – 47

7

Beta strand

51 – 56

6

Helix

59 – 61

3

Helix

62 – 71

10

Beta strand

75 – 79

5

Helix

81 – 93

13

Beta strand

100 – 109

10

Helix

110 – 121

12

Beta strand

125 – 134

10

Beta strand

138 – 144

7

Beta strand

147 – 153

7

Helix

155 – 159

5

Turn

162 – 167

6

Helix

169 – 177

9

Beta strand

179 – 184

6

Helix

185 – 189

5

Helix

192 – 204

13

Beta strand

208 – 212

5

Helix

216 – 220

5

Helix

223 – 229

7

Helix

230 – 232

3

Beta strand

234 – 239

6

Helix

240 – 250

11

Helix

257 – 266

10

Beta strand

277 – 282

6

Beta strand

285 – 290

6

Beta strand

295 – 298

4

Helix

311 – 327

17

Helix

332 – 347

16

Beta strand

349 – 352

4

Sequences

	Sequence		Length	Mass (Da)
	Q64640-1 [UniParc]. Last modified January 9, 2007. Version 3. Checksum: 437B83CC56E7A1C8	FASTA	361	40,134
	10 20 30 40 50 60 MAAADEPKPK KLKVEAPEAL SENVLFGMGN PLLDISAVVD KDFLDKYSLK PNDQILAEDK 70 80 90 100 110 120 HKELFDELVK KFKVEYHAGG STQNSMKVAQ WMIQEPHRAA TFFGCIGIDK FGEILKSKAA 130 140 150 160 170 180 DAHVDAHYYE QNEQPTGTCA ACITGGNRSL VANLAAANCY KKEKHLDLEN NWMLVEKARV 190 200 210 220 230 240 YYIAGFFLTV SPESVLKVAR YAAENNRTFT LNLSAPFISQ FFKEALMEVM PYVDILFGNE 250 260 270 280 290 300 TEAATFAREQ GFETKDIKEI ARKTQALPKV NSKRQRTVIF TQGRDDTIVA TGNDVTAFPV 310 320 330 340 350 360 LDQNQEEIVD TNGAGDAFVG GFLSQLVSNK PLTECIRAGH YAASVIIRRT GCTFPEKPDF H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the adenosine kinase gene from rat and human tissues." McNally T., Helfrich R.J., Cowart M., Dorwin S.A., Meuth J.L., Idler K.B., Klute K.A., Simmer R.L., Kowaluk E.A., Halbert D.N. Biochem. Biophys. Res. Commun. 231:645-650(1997) [PubMed: 9070863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[3]	"Cloning and characterization of cDNA for adenosine kinase from mammalian (Chinese hamster, mouse, human and rat) species. High frequency mutants of Chinese hamster ovary cells involve structural alterations in the gene." Singh B., Hao W., Wu Z.-C., Eigl B., Gupta R.S. Eur. J. Biochem. 241:564-571(1996) [PubMed: 8917457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-361, CHARACTERIZATION. Tissue: Liver.
[4]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 88-110 AND 119-148, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U90340 mRNA. Translation: AAB50236.1.
BC081712 mRNA. Translation: AAH81712.1.
U57042 mRNA. Translation: AAB03110.1.

IPI

IPI00214456.

PIR

JC5362.

RefSeq

NP_037027.2.

UniGene

Rn.64165

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LNJ	model	-	A	1-361	[»]

SMR

Q64640. Positions 22-361.

ModBase

Search...

Proteomic databases

PRIDE

Q64640.

Genome annotation databases

Ensembl

ENSRNOG00000012325. Rattus norvegicus. [Contig view]

GeneID

25368.

KEGG

rno:25368.

Organism-specific databases

RGD

2046. Adk.

Phylogenomic databases

HOVERGEN

Q64640.

Enzyme and pathway databases

BRENDA

2.7.1.20. 248.

Gene expression databases

ArrayExpress

Q64640.

GermOnline

ENSRNOG00000012325. Rattus norvegicus.

Family and domain databases

InterPro

IPR001805. Adenokinase.
IPR011611. Carb/pur_kinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
[Graphical view]

PANTHER

PTHR10584:SF24. Adenokinase. 1 hit.

Pfam

PF00294. PfkB. 1 hit.
[Graphical view]

PRINTS

PR00989. ADENOKINASE.

PROSITE

PS00583. PFKB_KINASES_1. False negative.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

Q64640.

NextBio

606373.

Entry information

Entry name

ADK_RAT

Accession

Primary (citable) accession number: Q64640
Secondary accession number(s): O09162, Q642G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)