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Q64632 (ITB4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin beta-4
Alternative name(s):
GP150
CD_antigen=CD104
Gene names
Name:Itgb4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1807 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility By similarity.

Subunit structure

Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6. Interacts (via cytoplasmic region) with COL17A1 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3(via N-terminus). Interacts (via cytoplasmic domain) with DST (via N-terminus). Interacts with RAC1 By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Cell membrane; Lipid-anchor By similarity. Cell junctionhemidesmosome By similarity. Note: Colocalizes with DST at the leading edge of migrating keratinocytes By similarity.

Domain

The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

Post-translational modification

Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secundary association with tertaspanins By similarity.

Sequence similarities

Belongs to the integrin beta chain family.

Contains 1 Calx-beta domain.

Contains 4 fibronectin type-III domains.

Contains 1 PSI domain.

Contains 1 VWFA domain.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell junction
Cell membrane
Membrane
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionIntegrin
Receptor
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Traceable author statement PubMed 8026337. Source: RGD

cell motility

Inferred from sequence or structural similarity. Source: UniProtKB

cell-matrix adhesion

Inferred from electronic annotation. Source: InterPro

hemidesmosome assembly

Inferred from electronic annotation. Source: InterPro

integrin-mediated signaling pathway

Traceable author statement PubMed 8026337. Source: RGD

multicellular organismal development

Inferred from electronic annotation. Source: InterPro

response to wounding

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcell cortex

Inferred from direct assay PubMed 16409251. Source: RGD

cell leading edge

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from direct assay PubMed 16409251. Source: RGD

extracellular space

Traceable author statement PubMed 8026337. Source: RGD

hemidesmosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Traceable author statement PubMed 8026337. Source: RGD

integrin complex

Traceable author statement PubMed 8026337. Source: RGD

membrane

Inferred from direct assay PubMed 16409251. Source: RGD

   Molecular_functioncell adhesion molecule binding

Traceable author statement PubMed 8026337. Source: RGD

receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 By similarity
Chain28 – 18071780Integrin beta-4
PRO_0000016347

Regions

Topological domain28 – 713686Extracellular Potential
Transmembrane714 – 73421Helical; Potential
Topological domain735 – 18071073Cytoplasmic Potential
Domain29 – 7345PSI
Domain131 – 340210VWFA
Repeat457 – 50347I
Repeat504 – 54340II
Repeat544 – 58239III
Repeat583 – 62139IV
Domain981 – 1086106Calx-beta
Domain1131 – 122090Fibronectin type-III 1
Domain1224 – 1323100Fibronectin type-III 2
Domain1514 – 160996Fibronectin type-III 3
Domain1627 – 172397Fibronectin type-III 4
Region457 – 621165Cysteine-rich tandem repeats
Region734 – 75118Palmitoylated on several cysteines By similarity

Amino acid modifications

Modified residue15141Phosphothreonine By similarity
Glycosylation3271N-linked (GlcNAc...) Potential
Glycosylation4921N-linked (GlcNAc...) Potential
Glycosylation5801N-linked (GlcNAc...) Potential
Glycosylation6191N-linked (GlcNAc...) Potential
Glycosylation6971N-linked (GlcNAc...) Potential
Disulfide bond30 ↔ 456 By similarity
Disulfide bond38 ↔ 48 By similarity
Disulfide bond41 ↔ 72 By similarity
Disulfide bond51 ↔ 61 By similarity
Disulfide bond245 ↔ 288 By similarity
Disulfide bond424 ↔ 673 By similarity
Disulfide bond453 ↔ 458 By similarity
Disulfide bond469 ↔ 480 By similarity
Disulfide bond477 ↔ 513 By similarity
Disulfide bond482 ↔ 491 By similarity
Disulfide bond493 ↔ 504 By similarity
Disulfide bond519 ↔ 524 By similarity
Disulfide bond521 ↔ 552 By similarity
Disulfide bond526 ↔ 537 By similarity
Disulfide bond558 ↔ 563 By similarity
Disulfide bond565 ↔ 574 By similarity
Disulfide bond576 ↔ 583 By similarity
Disulfide bond597 ↔ 602 By similarity
Disulfide bond599 ↔ 650 By similarity
Disulfide bond604 ↔ 616 By similarity
Disulfide bond628 ↔ 637 By similarity
Disulfide bond634 ↔ 708 By similarity
Disulfide bond653 ↔ 682 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64632 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2EF9BD4E345829A3

FASTA1,807200,589
        10         20         30         40         50         60 
MAGLCSSPWV KLLLAVVLSA GLPGNMANRC KKAQVKSCTE CIRVDKSCAY CTDELFKERR 

        70         80         90        100        110        120 
CNTQADVLAA GCRGESVLVM ESSLEITENI QIDTSLHRSQ VSPQGLQVRL RPGEERNFVF 

       130        140        150        160        170        180 
KVFEPLESPV DLYILMDFSN SMSDDLDNLK QMGQNLAKIL RQLTSDYTIG FGKFVDKVSV 

       190        200        210        220        230        240 
PQTDMRPEKL KEPWPNSDPP FSFKNVISLT ENVEEFWDKL QGERISGNLD APEGGFDAIL 

       250        260        270        280        290        300 
QTAVCTRDIG WRADSTHLLV FSTESAFHYE ADGANVLAGI MNRNDEKCHL DATGAYTQYK 

       310        320        330        340        350        360 
TQDYPSVPTL VRLLAKHNII PIFAVTNYSY SYYEKLHKYF PVSSLGVLQE DSSNIVELLE 

       370        380        390        400        410        420 
EAFYRIRSNL DIRALDSPRG LRTEVTSDTL QKTETGSFHI KRGEVGTYNV HLRAVEDIDG 

       430        440        450        460        470        480 
THVCQLAKED QRGNIHLKPS FSDGLRMDAS VICDMCACEL QKEVQSARCH YRGDFMCGHC 

       490        500        510        520        530        540 
VCNEGWSGKT CNCSTGSLSD TQPCLREGED KPCSGHGECQ CGRCVCYGEG RYEGHFCEYD 

       550        560        570        580        590        600 
NFQCPRTSGF LCNDRGRCSM GECVCEPGWT GRSCDCPLSN ATCIDSNGGI CNGLGFCECG 

       610        620        630        640        650        660 
RCHCNQRSSL YTDTTCEINY SAIRLGLCED LRSCVQCQAW GTGEKKGRTC EECNFKVKMV 

       670        680        690        700        710        720 
DELKKAEEVV EYCSFRDEDD DCTYSYTVEG DGSPGPNSTV LVHKKKDCLP APSWWLIPLL 

       730        740        750        760        770        780 
IFLLLLLVLL LLLCWKYCAC CKACLGLLPC CNQGHMVGFK EDHYMLRENL MASDHLDTPM 

       790        800        810        820        830        840 
LRSGNLKGRD TVRWKITNNV QRPGFATHAA SISPTELVPY GLSLRLGRLC TENLMKPGTR 

       850        860        870        880        890        900 
ECDQLRQEVE ENLNEVYRQV NGVHKLQQTK FRQQPNAGKK QDHTIVDTVL LAPRSAKQSL 

       910        920        930        940        950        960 
LKLTEKQVEQ GSFHELKVAP GYYTLTAEQD ARGMVEFQEG VELVDVRVPL FIRPEDDDEK 

       970        980        990       1000       1010       1020 
QLLVEAIDVP VGTATLGRRL VNITIIKEQA SGIVSFEQPE YSVSRGDQVA RIPVIRHILD 

      1030       1040       1050       1060       1070       1080 
NGKSQVSYST QDNTAHGHRD YVPVEGELLF YPGETWKELQ VKLLELQEVD SLLRGRQVRR 

      1090       1100       1110       1120       1130       1140 
FQVQLSNPKF GARLGQPNTA TVIIGEQDET DRSLINEISA SPPLPRGDLG APQNPNAKAA 

      1150       1160       1170       1180       1190       1200 
GSRKIHFNWL PPPGKPMGYR VKYWVQGDSE SEAHLLDSKV PSVELTNLYP YCDYEMKVCA 

      1210       1220       1230       1240       1250       1260 
YGAHGEGPYS SLVSCRTHQE VPSEPGRLAF NVVSSTVTQL SWAEPAETNG EITAYEVCYG 

      1270       1280       1290       1300       1310       1320 
LVNEDNRPIG PMKKVLVDNP KNRMLLIENL RESQPYRYTV KARNGAGWGP EREAIINLAT 

      1330       1340       1350       1360       1370       1380 
QPKRPMSIPI IPDIPIVDAQ GGEDYENFLM YSDDVLRSPA SSQRPSVSDD TEHLVNGRMD 

      1390       1400       1410       1420       1430       1440 
FAYPGSANSL HRMTAANVAY GTHLSPHQTH RMLSTSSTLT RDYHSLTRTD HSQSGTLPRD 

      1450       1460       1470       1480       1490       1500 
YSTLTSLSSQ GLPPIWEDGR SRLPLSWTLG SWSRAQMKGV PASRGSPDSI ILAGQSAAPS 

      1510       1520       1530       1540       1550       1560 
WGTDSRGAMG VPDTPTRLVF SALGPTSLKV SWQEPQCDRA LLGYSVEYQL LNGGEMHRLN 

      1570       1580       1590       1600       1610       1620 
IPNPGQTSVV VEDLLPNHSY VFRVRAQSQE GWGREREGVI TIESQVHPQS PLCPLPGSAF 

      1630       1640       1650       1660       1670       1680 
TLSTPSAPGP LVFTALSPDS LQLSWERPRR PNGDILGYLV TCEMAQGGGP ARTFRVDGDN 

      1690       1700       1710       1720       1730       1740 
PESRLTVPGL SENVPYKFKV QARTTEGFGP EREGIITIES QDGGPFPQLG SHSGLFQNPL 

      1750       1760       1770       1780       1790       1800 
QSEYSTVTST HSTTTTEPFL IDGLTLGTQR LEAGGSLTRH VTQEFVSRTL TTSGSLSTHM 


DQQFFQT 

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References

[1]"Cloning and sequence of the cDNA encoding the beta 4 integrin subunit in rat peripheral nerve."
Feltri M.L., Arona M., Scherer S.S., Wrabetz L.
Gene 186:299-304(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Sciatic nerve.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U60096 mRNA. Translation: AAC53094.1.
PIRJC6319.
RefSeqNP_037312.1. NM_013180.1.
UniGeneRn.198908.

3D structure databases

ProteinModelPortalQ64632.
SMRQ64632. Positions 1128-1322, 1504-1606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000048660.

PTM databases

PhosphoSiteQ64632.

Proteomic databases

PaxDbQ64632.
PRIDEQ64632.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25724.
KEGGrno:25724.

Organism-specific databases

CTD3691.
RGD2928. Itgb4.

Phylogenomic databases

eggNOGNOG303049.
HOGENOMHOG000231105.
HOVERGENHBG006189.
InParanoidQ64632.
KOK06525.
PhylomeDBQ64632.

Gene expression databases

GenevestigatorQ64632.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
3.40.50.410. 1 hit.
InterProIPR003644. Calx_beta.
IPR013032. EGF-like_CS.
IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR015812. Integrin_bsu.
IPR012013. Integrin_bsu-4.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF6. PTHR10082:SF6. 1 hit.
PfamPF03160. Calx-beta. 1 hit.
PF00041. fn3. 4 hits.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFPIRSF002513. Integrin_B4. 1 hit.
PRINTSPR01186. INTEGRINB.
SMARTSM00237. Calx_beta. 1 hit.
SM00060. FN3. 4 hits.
SM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF103575. SSF103575. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF53300. SSF53300. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS50853. FN3. 4 hits.
PS00243. INTEGRIN_BETA. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio607829.
PROQ64632.

Entry information

Entry nameITB4_RAT
AccessionPrimary (citable) accession number: Q64632
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families