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Protein

Integrin beta-4

Gene

Itgb4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility (By similarity).By similarity

GO - Molecular functioni

  1. cell adhesion molecule binding Source: RGD
  2. receptor activity Source: InterPro

GO - Biological processi

  1. cell adhesion Source: RGD
  2. cell-matrix adhesion Source: InterPro
  3. cell motility Source: UniProtKB
  4. hemidesmosome assembly Source: InterPro
  5. integrin-mediated signaling pathway Source: RGD
  6. multicellular organismal development Source: InterPro
  7. response to wounding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-4
Alternative name(s):
GP150
CD_antigen: CD104
Gene namesi
Name:Itgb4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2928. Itgb4.

Subcellular locationi

Cell membrane By similarity; Single-pass type I membrane protein By similarity. Cell membrane By similarity; Lipid-anchor By similarity. Cell junctionhemidesmosome By similarity
Note: Colocalizes with DST at the leading edge of migrating keratinocytes.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 713686ExtracellularSequence AnalysisAdd
BLAST
Transmembranei714 – 73421HelicalSequence AnalysisAdd
BLAST
Topological domaini735 – 18071073CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell cortex Source: RGD
  2. cell leading edge Source: UniProtKB
  3. cytoplasm Source: RGD
  4. extracellular space Source: RGD
  5. hemidesmosome Source: UniProtKB-SubCell
  6. integral component of membrane Source: RGD
  7. integrin complex Source: RGD
  8. membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Chaini28 – 18071780Integrin beta-4PRO_0000016347Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 456By similarity
Disulfide bondi38 ↔ 48By similarity
Disulfide bondi41 ↔ 72By similarity
Disulfide bondi51 ↔ 61By similarity
Disulfide bondi245 ↔ 288By similarity
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi424 ↔ 673By similarity
Disulfide bondi453 ↔ 458By similarity
Disulfide bondi469 ↔ 480By similarity
Disulfide bondi477 ↔ 513By similarity
Disulfide bondi482 ↔ 491By similarity
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi493 ↔ 504By similarity
Disulfide bondi519 ↔ 524By similarity
Disulfide bondi521 ↔ 552By similarity
Disulfide bondi526 ↔ 537By similarity
Disulfide bondi558 ↔ 563By similarity
Disulfide bondi565 ↔ 574By similarity
Disulfide bondi576 ↔ 583By similarity
Glycosylationi580 – 5801N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi597 ↔ 602By similarity
Disulfide bondi599 ↔ 650By similarity
Disulfide bondi604 ↔ 616By similarity
Glycosylationi619 – 6191N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi628 ↔ 637By similarity
Disulfide bondi634 ↔ 708By similarity
Disulfide bondi653 ↔ 682By similarity
Glycosylationi697 – 6971N-linked (GlcNAc...)Sequence Analysis
Modified residuei1514 – 15141PhosphothreonineBy similarity

Post-translational modificationi

Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secundary association with tertaspanins (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ64632.
PRIDEiQ64632.

PTM databases

PhosphoSiteiQ64632.

Expressioni

Gene expression databases

GenevestigatoriQ64632.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6. Interacts (via cytoplasmic region) with COL17A1 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus). Interacts (via cytoplasmic domain) with DST (via N-terminus). Interacts with RAC1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048660.

Structurei

3D structure databases

ProteinModelPortaliQ64632.
SMRiQ64632. Positions 1128-1322, 1504-1606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 7345PSIAdd
BLAST
Domaini131 – 340210VWFAAdd
BLAST
Repeati457 – 50347IAdd
BLAST
Repeati504 – 54340IIAdd
BLAST
Repeati544 – 58239IIIAdd
BLAST
Repeati583 – 62139IVAdd
BLAST
Domaini981 – 1086106Calx-betaAdd
BLAST
Domaini1131 – 122090Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1224 – 1323100Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini1514 – 160996Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini1627 – 172397Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni457 – 621165Cysteine-rich tandem repeatsAdd
BLAST
Regioni734 – 75118Palmitoylated on several cysteinesBy similarityAdd
BLAST

Domaini

The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

Sequence similaritiesi

Belongs to the integrin beta chain family.Curated
Contains 1 Calx-beta domain.Curated
Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 PSI domain.Curated
Contains 1 VWFA domain.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG303049.
HOGENOMiHOG000231105.
HOVERGENiHBG006189.
InParanoidiQ64632.
KOiK06525.
PhylomeDBiQ64632.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
3.40.50.410. 1 hit.
InterProiIPR003644. Calx_beta.
IPR013032. EGF-like_CS.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR015812. Integrin_bsu.
IPR012013. Integrin_bsu-4.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF6. PTHR10082:SF6. 1 hit.
PfamiPF03160. Calx-beta. 1 hit.
PF00041. fn3. 4 hits.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002513. Integrin_B4. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00237. Calx_beta. 1 hit.
SM00060. FN3. 4 hits.
SM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF53300. SSF53300. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS50853. FN3. 4 hits.
PS00243. INTEGRIN_BETA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64632-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGLCSSPWV KLLLAVVLSA GLPGNMANRC KKAQVKSCTE CIRVDKSCAY
60 70 80 90 100
CTDELFKERR CNTQADVLAA GCRGESVLVM ESSLEITENI QIDTSLHRSQ
110 120 130 140 150
VSPQGLQVRL RPGEERNFVF KVFEPLESPV DLYILMDFSN SMSDDLDNLK
160 170 180 190 200
QMGQNLAKIL RQLTSDYTIG FGKFVDKVSV PQTDMRPEKL KEPWPNSDPP
210 220 230 240 250
FSFKNVISLT ENVEEFWDKL QGERISGNLD APEGGFDAIL QTAVCTRDIG
260 270 280 290 300
WRADSTHLLV FSTESAFHYE ADGANVLAGI MNRNDEKCHL DATGAYTQYK
310 320 330 340 350
TQDYPSVPTL VRLLAKHNII PIFAVTNYSY SYYEKLHKYF PVSSLGVLQE
360 370 380 390 400
DSSNIVELLE EAFYRIRSNL DIRALDSPRG LRTEVTSDTL QKTETGSFHI
410 420 430 440 450
KRGEVGTYNV HLRAVEDIDG THVCQLAKED QRGNIHLKPS FSDGLRMDAS
460 470 480 490 500
VICDMCACEL QKEVQSARCH YRGDFMCGHC VCNEGWSGKT CNCSTGSLSD
510 520 530 540 550
TQPCLREGED KPCSGHGECQ CGRCVCYGEG RYEGHFCEYD NFQCPRTSGF
560 570 580 590 600
LCNDRGRCSM GECVCEPGWT GRSCDCPLSN ATCIDSNGGI CNGLGFCECG
610 620 630 640 650
RCHCNQRSSL YTDTTCEINY SAIRLGLCED LRSCVQCQAW GTGEKKGRTC
660 670 680 690 700
EECNFKVKMV DELKKAEEVV EYCSFRDEDD DCTYSYTVEG DGSPGPNSTV
710 720 730 740 750
LVHKKKDCLP APSWWLIPLL IFLLLLLVLL LLLCWKYCAC CKACLGLLPC
760 770 780 790 800
CNQGHMVGFK EDHYMLRENL MASDHLDTPM LRSGNLKGRD TVRWKITNNV
810 820 830 840 850
QRPGFATHAA SISPTELVPY GLSLRLGRLC TENLMKPGTR ECDQLRQEVE
860 870 880 890 900
ENLNEVYRQV NGVHKLQQTK FRQQPNAGKK QDHTIVDTVL LAPRSAKQSL
910 920 930 940 950
LKLTEKQVEQ GSFHELKVAP GYYTLTAEQD ARGMVEFQEG VELVDVRVPL
960 970 980 990 1000
FIRPEDDDEK QLLVEAIDVP VGTATLGRRL VNITIIKEQA SGIVSFEQPE
1010 1020 1030 1040 1050
YSVSRGDQVA RIPVIRHILD NGKSQVSYST QDNTAHGHRD YVPVEGELLF
1060 1070 1080 1090 1100
YPGETWKELQ VKLLELQEVD SLLRGRQVRR FQVQLSNPKF GARLGQPNTA
1110 1120 1130 1140 1150
TVIIGEQDET DRSLINEISA SPPLPRGDLG APQNPNAKAA GSRKIHFNWL
1160 1170 1180 1190 1200
PPPGKPMGYR VKYWVQGDSE SEAHLLDSKV PSVELTNLYP YCDYEMKVCA
1210 1220 1230 1240 1250
YGAHGEGPYS SLVSCRTHQE VPSEPGRLAF NVVSSTVTQL SWAEPAETNG
1260 1270 1280 1290 1300
EITAYEVCYG LVNEDNRPIG PMKKVLVDNP KNRMLLIENL RESQPYRYTV
1310 1320 1330 1340 1350
KARNGAGWGP EREAIINLAT QPKRPMSIPI IPDIPIVDAQ GGEDYENFLM
1360 1370 1380 1390 1400
YSDDVLRSPA SSQRPSVSDD TEHLVNGRMD FAYPGSANSL HRMTAANVAY
1410 1420 1430 1440 1450
GTHLSPHQTH RMLSTSSTLT RDYHSLTRTD HSQSGTLPRD YSTLTSLSSQ
1460 1470 1480 1490 1500
GLPPIWEDGR SRLPLSWTLG SWSRAQMKGV PASRGSPDSI ILAGQSAAPS
1510 1520 1530 1540 1550
WGTDSRGAMG VPDTPTRLVF SALGPTSLKV SWQEPQCDRA LLGYSVEYQL
1560 1570 1580 1590 1600
LNGGEMHRLN IPNPGQTSVV VEDLLPNHSY VFRVRAQSQE GWGREREGVI
1610 1620 1630 1640 1650
TIESQVHPQS PLCPLPGSAF TLSTPSAPGP LVFTALSPDS LQLSWERPRR
1660 1670 1680 1690 1700
PNGDILGYLV TCEMAQGGGP ARTFRVDGDN PESRLTVPGL SENVPYKFKV
1710 1720 1730 1740 1750
QARTTEGFGP EREGIITIES QDGGPFPQLG SHSGLFQNPL QSEYSTVTST
1760 1770 1780 1790 1800
HSTTTTEPFL IDGLTLGTQR LEAGGSLTRH VTQEFVSRTL TTSGSLSTHM

DQQFFQT
Length:1,807
Mass (Da):200,589
Last modified:November 1, 1996 - v1
Checksum:i2EF9BD4E345829A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60096 mRNA. Translation: AAC53094.1.
PIRiJC6319.
RefSeqiNP_037312.1. NM_013180.1.
UniGeneiRn.198908.

Genome annotation databases

GeneIDi25724.
KEGGirno:25724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60096 mRNA. Translation: AAC53094.1.
PIRiJC6319.
RefSeqiNP_037312.1. NM_013180.1.
UniGeneiRn.198908.

3D structure databases

ProteinModelPortaliQ64632.
SMRiQ64632. Positions 1128-1322, 1504-1606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048660.

PTM databases

PhosphoSiteiQ64632.

Proteomic databases

PaxDbiQ64632.
PRIDEiQ64632.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25724.
KEGGirno:25724.

Organism-specific databases

CTDi3691.
RGDi2928. Itgb4.

Phylogenomic databases

eggNOGiNOG303049.
HOGENOMiHOG000231105.
HOVERGENiHBG006189.
InParanoidiQ64632.
KOiK06525.
PhylomeDBiQ64632.

Miscellaneous databases

NextBioi607829.
PROiQ64632.

Gene expression databases

GenevestigatoriQ64632.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
3.40.50.410. 1 hit.
InterProiIPR003644. Calx_beta.
IPR013032. EGF-like_CS.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR015812. Integrin_bsu.
IPR012013. Integrin_bsu-4.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF6. PTHR10082:SF6. 1 hit.
PfamiPF03160. Calx-beta. 1 hit.
PF00041. fn3. 4 hits.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002513. Integrin_B4. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00237. Calx_beta. 1 hit.
SM00060. FN3. 4 hits.
SM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF53300. SSF53300. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS50853. FN3. 4 hits.
PS00243. INTEGRIN_BETA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence of the cDNA encoding the beta 4 integrin subunit in rat peripheral nerve."
    Feltri M.L., Arona M., Scherer S.S., Wrabetz L.
    Gene 186:299-304(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Sciatic nerve.

Entry informationi

Entry nameiITB4_RAT
AccessioniPrimary (citable) accession number: Q64632
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.