Q64627 (NEUR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sialidase-2
EC=3.2.1.18

Alternative name(s):
Cytosolic sialidase
N-acetyl-alpha-neuraminidase 2

Gene names

Name:

Neu2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes sialylated compounds.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 2 BNR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of myoblast differentiation Inferred from direct assay PubMed 12860410. Source: RGD positive regulation of myotube differentiation Inferred from mutant phenotype PubMed 8630046. Source: RGD response to ethanol Inferred from expression pattern PubMed 16713441. Source: RGD
Cellular_component	cytosol Inferred from direct assay PubMed 12860410. Source: RGD
Molecular_function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-sialidase activity Inferred from direct assay PubMed 15710387. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Sialidase-2		PRO_0000208901
Regions
	Repeat	127 – 138	12	BNR 1
	Repeat	197 – 208	12	BNR 2
	Motif	20 – 23	4	FRIP motif
Sites
	Active site	46	1	Proton acceptor By similarity
	Active site	333	1	Nucleophile By similarity
	Active site	354	1	Potential
	Binding site	21	1	Substrate By similarity
	Binding site	237	1	Substrate Potential
	Binding site	303	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64627 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 55583C7043CA9784	FASTA	379	42,382
	10 20 30 40 50 60 METCPVLQKE TLFHTEVYAY RIPALLYLKK QKTLLAFAEK RASRTDEHAE LIVLRRGSYN 70 80 90 100 110 120 GATNHVKWQP EEVVTQAQLE GHRSMNPCPL YDKQTKTLFL FFIAVPGRVS EQHQLQTRVN 130 140 150 160 170 180 VTRLCRVTST DYGMNWSPVQ DLTETTIGST HQDWATFAVG PGHCLQLRNR AGSLLVPAYA 190 200 210 220 230 240 YRKLHPVHKP TPFAFCFISL DHGHTWELGN FVSENSLECQ VAEVGTGAHR VVYLNARSFI 250 260 270 280 290 300 GARVQAQSPN DGLDFQDNQV VSKLVEPPHG CHGSVVAFHS PTSKPDCLRH VAAYTHPTDS 310 320 330 340 350 360 RNRTNLGVYL NQTPLDPTAW SEPTLLATGT CAYSDLQIWG LGPDGSPQFG CLYESGNYDE 370 IIFLMFTLKQ AFPTVHGAQ

References

[1]	"Molecular cloning and expression of cDNA encoding rat skeletal muscle cytosolic sialidase." Miyagi T., Konno K., Emori Y., Kawasaki H., Suzuki K., Yasui A., Tsuiki S. J. Biol. Chem. 268:26435-26440(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Wistar. Tissue: Skeletal muscle.
[2]	"Genomic organization and the 5'-upstream sequence of the rat cytosolic sialidase gene." Sato K., Miyagi T. Glycobiology 5:511-516(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16300 mRNA. Translation: BAA03805.1. D50606 Genomic DNA. Translation: BAA09169.1.
IPI	IPI00214420.
PIR	A49679.
UniGene	Rn.9731.
3D structure databases
ProteinModelPortal	Q64627.
ModBase	Search...
Protein family/group databases
CAZy	GH33. Glycoside Hydrolase Family 33.
PTM databases
PhosphoSite	Q64627.
Proteomic databases
PaxDb	Q64627.
PRIDE	Q64627.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:3164. rat.
Organism-specific databases
RGD	3164. Neu2.
Phylogenomic databases
eggNOG	NOG84455.
HOGENOM	HOG000233778.
HOVERGEN	HBG052608.
InParanoid	Q64627.
OrthoDB	EOG4B5P5G.
Gene expression databases
ArrayExpress	Q64627.
Genevestigator	Q64627.
GermOnline	ENSRNOG00000016962. Rattus norvegicus.
Family and domain databases
Gene3D	2.120.10.10. 1 hit.
InterPro	IPR026945. Sialidase-2. IPR026856. Sialidase_fam. IPR011040. Sialidases. [Graphical view]
PANTHER	PTHR10628. PTHR10628. 1 hit. PTHR10628:SF6. PTHR10628:SF6. 1 hit.
SUPFAM	SSF50939. Sialidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NEUR2_RAT

Accession

Primary (citable) accession number: Q64627
Secondary accession number(s): Q63705

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program