Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q64627 (NEUR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase-2

EC=3.2.1.18
Alternative name(s):
Cytosolic sialidase
N-acetyl-alpha-neuraminidase 2
Gene names
Name:Neu2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides. Ref.1

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. Ref.1

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Detected in skeletal muscle. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 2 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Sialidase-2
PRO_0000208901

Regions

Repeat127 – 13812BNR 1
Repeat197 – 20812BNR 2
Motif20 – 234FRIP motif

Sites

Active site461Proton acceptor By similarity
Active site3331Nucleophile By similarity
Active site3541 Potential
Binding site211Substrate By similarity
Binding site411Substrate By similarity
Binding site1791Substrate By similarity
Binding site1811Substrate By similarity
Binding site2181Substrate By similarity
Binding site2371Substrate Potential
Binding site3031Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64627 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 55583C7043CA9784

FASTA37942,382
        10         20         30         40         50         60 
METCPVLQKE TLFHTEVYAY RIPALLYLKK QKTLLAFAEK RASRTDEHAE LIVLRRGSYN 

        70         80         90        100        110        120 
GATNHVKWQP EEVVTQAQLE GHRSMNPCPL YDKQTKTLFL FFIAVPGRVS EQHQLQTRVN 

       130        140        150        160        170        180 
VTRLCRVTST DYGMNWSPVQ DLTETTIGST HQDWATFAVG PGHCLQLRNR AGSLLVPAYA 

       190        200        210        220        230        240 
YRKLHPVHKP TPFAFCFISL DHGHTWELGN FVSENSLECQ VAEVGTGAHR VVYLNARSFI 

       250        260        270        280        290        300 
GARVQAQSPN DGLDFQDNQV VSKLVEPPHG CHGSVVAFHS PTSKPDCLRH VAAYTHPTDS 

       310        320        330        340        350        360 
RNRTNLGVYL NQTPLDPTAW SEPTLLATGT CAYSDLQIWG LGPDGSPQFG CLYESGNYDE 

       370 
IIFLMFTLKQ AFPTVHGAQ 

« Hide

References

[1]"Molecular cloning and expression of cDNA encoding rat skeletal muscle cytosolic sialidase."
Miyagi T., Konno K., Emori Y., Kawasaki H., Suzuki K., Yasui A., Tsuiki S.
J. Biol. Chem. 268:26435-26440(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
Strain: Wistar.
Tissue: Skeletal muscle.
[2]"Genomic organization and the 5'-upstream sequence of the rat cytosolic sialidase gene."
Sato K., Miyagi T.
Glycobiology 5:511-516(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67.
Strain: Sprague-Dawley.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16300 mRNA. Translation: BAA03805.1.
D50606 Genomic DNA. Translation: BAA09169.1.
PIRA49679.
UniGeneRn.9731.

3D structure databases

ProteinModelPortalQ64627.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

PTM databases

PhosphoSiteQ64627.

Proteomic databases

PaxDbQ64627.
PRIDEQ64627.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3164. rat.

Organism-specific databases

RGD3164. Neu2.

Phylogenomic databases

eggNOGNOG84455.
HOGENOMHOG000233778.
HOVERGENHBG052608.
InParanoidQ64627.
PhylomeDBQ64627.

Gene expression databases

GenevestigatorQ64627.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR026945. Sialidase-2.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF6. PTHR10628:SF6. 1 hit.
SUPFAMSSF50939. SSF50939. 1 hit.
ProtoNetSearch...

Other

PROQ64627.

Entry information

Entry nameNEUR2_RAT
AccessionPrimary (citable) accession number: Q64627
Secondary accession number(s): Q63705
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries