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Protein

Dual specificity protein phosphatase 1

Gene

Dusp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei258Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cellular response to hormone stimulus Source: RGD
  • endoderm formation Source: GO_Central
  • inactivation of MAPK activity Source: UniProtKB
  • intracellular signal transduction Source: RGD
  • mitotic cell cycle arrest Source: UniProtKB
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of DNA biosynthetic process Source: RGD
  • negative regulation of ERK1 and ERK2 cascade Source: RGD
  • negative regulation of MAPK cascade Source: UniProtKB
  • negative regulation of MAP kinase activity Source: UniProtKB
  • negative regulation of meiotic cell cycle Source: UniProtKB
  • peptidyl-threonine dephosphorylation Source: UniProtKB
  • peptidyl-tyrosine dephosphorylation Source: UniProtKB
  • positive regulation of apoptotic process Source: RGD
  • protein dephosphorylation Source: RGD
  • regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
  • response to calcium ion Source: RGD
  • response to cAMP Source: RGD
  • response to estradiol Source: RGD
  • response to glucocorticoid Source: RGD
  • response to hydrogen peroxide Source: RGD
  • response to light stimulus Source: RGD
  • response to organic substance Source: RGD
  • response to retinoic acid Source: RGD
  • response to testosterone Source: RGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle

Enzyme and pathway databases

ReactomeiR-RNO-112409 RAF-independent MAPK1/3 activation
R-RNO-5675221 Negative regulation of MAPK pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 1 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Mitogen-activated protein kinase phosphatase 1
Short name:
MAP kinase phosphatase 1
Short name:
MKP-1
Protein-tyrosine phosphatase CL100
Protein-tyrosine phosphatase non-receptor type 16
Gene namesi
Name:Dusp1
Synonyms:Cl100, Mkp1, Ptpn16
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi620897 Dusp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947921 – 367Dual specificity protein phosphatase 1Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei359Phosphoserine; by MAPK1 and MAPK3By similarity1
Modified residuei364Phosphoserine; by MAPK1 and MAPK3By similarity1

Post-translational modificationi

Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64623
PRIDEiQ64623

Expressioni

Tissue specificityi

Brain. High level expression seen in the cingulate gyrus within the retrospinal cortex, ventral and medial divisions of the anterior thalamus and the medial geniculate nucleus. Expressed at moderate levels in the parietal and temporal cortex. Expressed in the cerebellum.3 Publications

Inductioni

Exhibits night/day variations with a 100-fold increased expression at night in the pineal gland (at protein level). Up-regulation is due to a large degree to the release of norepinephrine from nerve terminals in the pineal gland and cAMP signaling pathway.2 Publications

Gene expression databases

BgeeiENSRNOG00000003977
GenevisibleiQ64623 RN

Interactioni

Protein-protein interaction databases

BioGridi250421, 1 interactor
STRINGi10116.ENSRNOP00000005383

Structurei

3D structure databases

ProteinModelPortaliQ64623
SMRiQ64623
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 137RhodanesePROSITE-ProRule annotationAdd BLAST118
Domaini175 – 367Tyrosine-protein phosphataseAdd BLAST193

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOGENOMiHOG000294080
HOVERGENiHBG007347
InParanoidiQ64623
KOiK21278
OMAiMVIMEVP
OrthoDBiEOG091G0249
PhylomeDBiQ64623
TreeFamiTF105122

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.40.250.10, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR020417 Atypical_DUSP
IPR020420 Atypical_DUSP_famB
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR008343 MKP
IPR029021 Prot-tyrosine_phosphatase-like
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF00581 Rhodanese, 1 hit
PIRSFiPIRSF000939 MAPK_Ptase, 1 hit
PRINTSiPR01908 ADSPHPHTASE
PR01910 ADSPHPHTASEB
PR01764 MAPKPHPHTASE
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SM00404 PTPc_motif, 1 hit
SM00450 RHOD, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
SSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequencei

Sequence statusi: Complete.

Q64623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVMEVGILDA GGLRALLRER AAQCLLLDCR SFFAFNAGHI VGSVNVRFST
60 70 80 90 100
IVRRRAKGAM GLEHIVPNTE LRGRLLAGAY HAVVLLDERS AALDGAKRDG
110 120 130 140 150
TLALAAGALC REARSTQVFF LQGGYEAFSA SCPELCSKQS TPMGLSLPLS
160 170 180 190 200
TSVPDSAESG CSSCSTPLYD QGGPVEILSF LYLGSAYHAS RKDMLDALGI
210 220 230 240 250
TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA IDFIDSIKDA
260 270 280 290 300
GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS
310 320 330 340 350
FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHPT
360
NSALNYLQSP ITTSPSC
Length:367
Mass (Da):39,541
Last modified:November 1, 1996 - v1
Checksum:i5112ADF290499139
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84004 mRNA Translation: CAA58828.1
AF357203 Genomic DNA Translation: AAK55327.1
PIRiS52265
RefSeqiNP_446221.2, NM_053769.3
UniGeneiRn.98260

Genome annotation databases

EnsembliENSRNOT00000005383; ENSRNOP00000005383; ENSRNOG00000003977
GeneIDi114856
KEGGirno:114856
UCSCiRGD:620897 rat

Similar proteinsi

Entry informationi

Entry nameiDUS1_RAT
AccessioniPrimary (citable) accession number: Q64623
Secondary accession number(s): Q548G6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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