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Q64623

- DUS1_RAT

UniProt

Q64623 - DUS1_RAT

Protein

Dual specificity protein phosphatase 1

Gene

Dusp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei258 – 2581Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: UniProtKB
    2. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
    3. protein tyrosine/threonine phosphatase activity Source: RGD
    4. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to hormone stimulus Source: RGD
    2. endoderm formation Source: RefGenome
    3. inactivation of MAPK activity Source: UniProtKB
    4. intracellular signal transduction Source: RGD
    5. mitotic cell cycle arrest Source: UniProtKB
    6. negative regulation of apoptotic process Source: RGD
    7. negative regulation of DNA biosynthetic process Source: RGD
    8. negative regulation of ERK1 and ERK2 cascade Source: RGD
    9. negative regulation of MAPK cascade Source: UniProtKB
    10. negative regulation of MAP kinase activity Source: UniProtKB
    11. negative regulation of meiotic cell cycle Source: UniProtKB
    12. peptidyl-threonine dephosphorylation Source: UniProtKB
    13. peptidyl-tyrosine dephosphorylation Source: UniProtKB
    14. positive regulation of apoptotic process Source: RGD
    15. protein dephosphorylation Source: RGD
    16. regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
    17. response to calcium ion Source: RGD
    18. response to cAMP Source: RGD
    19. response to estradiol Source: RGD
    20. response to glucocorticoid Source: RGD
    21. response to hydrogen peroxide Source: RGD
    22. response to light stimulus Source: RGD
    23. response to organic substance Source: RGD
    24. response to retinoic acid Source: RGD
    25. response to testosterone Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 1 (EC:3.1.3.16, EC:3.1.3.48)
    Alternative name(s):
    Mitogen-activated protein kinase phosphatase 1
    Short name:
    MAP kinase phosphatase 1
    Short name:
    MKP-1
    Protein-tyrosine phosphatase CL100
    Protein-tyrosine phosphatase non-receptor type 16
    Gene namesi
    Name:Dusp1
    Synonyms:Cl100, Mkp1, Ptpn16
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi620897. Dusp1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: RGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Dual specificity protein phosphatase 1PRO_0000094792Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei359 – 3591Phosphoserine; by MAPK1 and MAPK3By similarity
    Modified residuei364 – 3641Phosphoserine; by MAPK1 and MAPK3By similarity

    Post-translational modificationi

    Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ64623.

    Expressioni

    Tissue specificityi

    Brain. High level expression seen in the cingulate gyrus within the retrospinal cortex, ventral and medial divisions of the anterior thalamus and the medial geniculate nucleus. Expressed at moderate levels in the parietal and temporal cortex. Expressed in the cerebellum.3 Publications

    Inductioni

    Exhibits night/day variations with a 100-fold increased expression at night in the pineal gland (at protein level). Up-regulation is due to a large degree to the release of norepinephrine from nerve terminals in the pineal gland and cAMP signaling pathway.2 Publications

    Gene expression databases

    GenevestigatoriQ64623.

    Interactioni

    Protein-protein interaction databases

    BioGridi250421. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64623.
    SMRiQ64623. Positions 171-313.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 137118RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini175 – 367193Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2453.
    GeneTreeiENSGT00700000104321.
    HOGENOMiHOG000294080.
    HOVERGENiHBG007347.
    InParanoidiQ64623.
    KOiK04459.
    OMAiSFNSSHI.
    OrthoDBiEOG75MVWD.
    PhylomeDBiQ64623.
    TreeFamiTF105122.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR020417. Atypical_DUSP.
    IPR020420. Atypical_DUSP_famB.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000939. MAPK_Ptase. 1 hit.
    PRINTSiPR01908. ADSPHPHTASE.
    PR01910. ADSPHPHTASEB.
    PR01764. MAPKPHPHTASE.
    SMARTiSM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64623-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVMEVGILDA GGLRALLRER AAQCLLLDCR SFFAFNAGHI VGSVNVRFST    50
    IVRRRAKGAM GLEHIVPNTE LRGRLLAGAY HAVVLLDERS AALDGAKRDG 100
    TLALAAGALC REARSTQVFF LQGGYEAFSA SCPELCSKQS TPMGLSLPLS 150
    TSVPDSAESG CSSCSTPLYD QGGPVEILSF LYLGSAYHAS RKDMLDALGI 200
    TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA IDFIDSIKDA 250
    GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS 300
    FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHPT 350
    NSALNYLQSP ITTSPSC 367
    Length:367
    Mass (Da):39,541
    Last modified:November 1, 1996 - v1
    Checksum:i5112ADF290499139
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84004 mRNA. Translation: CAA58828.1.
    AF357203 Genomic DNA. Translation: AAK55327.1.
    PIRiS52265.
    RefSeqiNP_446221.2. NM_053769.3.
    UniGeneiRn.98260.

    Genome annotation databases

    EnsembliENSRNOT00000005383; ENSRNOP00000005383; ENSRNOG00000003977.
    GeneIDi114856.
    KEGGirno:114856.
    UCSCiRGD:620897. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84004 mRNA. Translation: CAA58828.1 .
    AF357203 Genomic DNA. Translation: AAK55327.1 .
    PIRi S52265.
    RefSeqi NP_446221.2. NM_053769.3.
    UniGenei Rn.98260.

    3D structure databases

    ProteinModelPortali Q64623.
    SMRi Q64623. Positions 171-313.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250421. 1 interaction.

    Proteomic databases

    PRIDEi Q64623.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000005383 ; ENSRNOP00000005383 ; ENSRNOG00000003977 .
    GeneIDi 114856.
    KEGGi rno:114856.
    UCSCi RGD:620897. rat.

    Organism-specific databases

    CTDi 1843.
    RGDi 620897. Dusp1.

    Phylogenomic databases

    eggNOGi COG2453.
    GeneTreei ENSGT00700000104321.
    HOGENOMi HOG000294080.
    HOVERGENi HBG007347.
    InParanoidi Q64623.
    KOi K04459.
    OMAi SFNSSHI.
    OrthoDBi EOG75MVWD.
    PhylomeDBi Q64623.
    TreeFami TF105122.

    Miscellaneous databases

    NextBioi 618903.
    PROi Q64623.

    Gene expression databases

    Genevestigatori Q64623.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR020417. Atypical_DUSP.
    IPR020420. Atypical_DUSP_famB.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000939. MAPK_Ptase. 1 hit.
    PRINTSi PR01908. ADSPHPHTASE.
    PR01910. ADSPHPHTASEB.
    PR01764. MAPKPHPHTASE.
    SMARTi SM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Pathways regulating CL100 gene expression in pituitary cells."
      Muda M., Schlegel W., Arkinstall S.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Lung.
    2. "MAP kinase phosphatase-1 gene transcription in rat neuroendocrine cells is modulated by a calcium-sensitive block to elongation in the first exon."
      Ryser S., Tortola S., van Haasteren G., Muda M., Li S., Schlegel W.
      J. Biol. Chem. 276:33319-33327(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Wistar Furth.
    3. "Isolation and characterization of a human dual specificity protein-tyrosine phosphatase gene."
      Kwak S.P., Hakes D.J., Martell K.J., Dixon J.E.
      J. Biol. Chem. 269:3596-3604(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Mitogen-activated protein kinase phosphatase-1 (MKP-1): >100-fold nocturnal and norepinephrine-induced changes in the rat pineal gland."
      Price D.M., Chik C.L., Terriff D., Weller J., Humphries A., Carter D.A., Klein D.C., Ho A.K.
      FEBS Lett. 577:220-226(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    5. Cited for: TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiDUS1_RAT
    AccessioniPrimary (citable) accession number: Q64623
    Secondary accession number(s): Q548G6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3