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Protein

Serine/threonine-protein phosphatase 6 catalytic subunit

Gene

Ppp6c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic subunit of protein phosphatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7 (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Manganese 1By similarity
Metal bindingi55 – 551Manganese 1By similarity
Metal bindingi81 – 811Manganese 1By similarity
Metal bindingi81 – 811Manganese 2By similarity
Metal bindingi113 – 1131Manganese 2By similarity
Active sitei114 – 1141Proton donorBy similarity
Metal bindingi163 – 1631Manganese 2By similarity
Metal bindingi237 – 2371Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: RGD

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. protein dephosphorylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 6 catalytic subunit (EC:3.1.3.16)
Short name:
PP6C
Alternative name(s):
Protein phosphatase V
Short name:
PP-V
Cleaved into the following chain:
Gene namesi
Name:Ppp6c
Synonyms:Ppv
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi708460. Ppp6c.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Serine/threonine-protein phosphatase 6 catalytic subunitPRO_0000058879Add
BLAST
Initiator methioninei1 – 11Removed; alternateBy similarity
Chaini2 – 305304Serine/threonine-protein phosphatase 6 catalytic subunit, N-terminally processedPRO_0000424506Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ64620.

PTM databases

PhosphoSiteiQ64620.

Expressioni

Tissue specificityi

Highest levels found in spleen, brain and lung.1 Publication

Gene expression databases

GenevestigatoriQ64620.

Interactioni

Subunit structurei

Protein phosphatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed by the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). Interacts with IGBP1, MAP3K7, NFKBIE, PPP6R1, PPP6R2 and PPP6R3 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ64620. 2 interactions.
STRINGi10116.ENSRNOP00000021176.

Structurei

3D structure databases

ProteinModelPortaliQ64620.
SMRiQ64620. Positions 5-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00550000074961.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiQ64620.
KOiK15498.
OMAiENWAVSP.
OrthoDBiEOG74N5H2.
PhylomeDBiQ64620.
TreeFamiTF105563.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64620-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPLDLDKYV EIARQCKYLP ENDLKRLCDY VCDLLLEESN VQPVSTPVTV
60 70 80 90 100
CGDIHGQFYD LCELFRTGGQ VPDTNYIFMG DFVDRGYYSL ETFTYLLALK
110 120 130 140 150
AKWPDRITLL RGNHESRQIT QVYGFYDECQ TKYGNANAWR YCTKVFDMLT
160 170 180 190 200
VAALIDEQIL CVHGGLSPDI KTLDQIRTIE RNQEIPHKGA FCDLVWSDPE
210 220 230 240 250
DVDTWAISPR GAGWLFGAKV TNEFVHINNL KLICRAHQLV HEGYKFMFDE
260 270 280 290 300
KLVTVWSAPN YCYRCGNIAS IMVFKDVNTR EPKLFRAVPD SERVIPPRTT

TPYFL
Length:305
Mass (Da):35,159
Last modified:January 15, 2008 - v2
Checksum:i662969DE1B2EF490
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721P → L in CAA54453. (PubMed:8077208)Curated
Sequence conflicti87 – 871Y → H in CAA54453. (PubMed:8077208)Curated
Sequence conflicti91 – 911E → V in CAA54453. (PubMed:8077208)Curated
Sequence conflicti285 – 2851F → N in CAA54453. (PubMed:8077208)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77236 mRNA. Translation: CAA54453.1.
BC078747 mRNA. Translation: AAH78747.1.
PIRiB55346.
RefSeqiNP_598273.2. NM_133589.2.
UniGeneiRn.9573.

Genome annotation databases

EnsembliENSRNOT00000021176; ENSRNOP00000021176; ENSRNOG00000015145.
GeneIDi171121.
KEGGirno:171121.
UCSCiRGD:708460. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77236 mRNA. Translation: CAA54453.1.
BC078747 mRNA. Translation: AAH78747.1.
PIRiB55346.
RefSeqiNP_598273.2. NM_133589.2.
UniGeneiRn.9573.

3D structure databases

ProteinModelPortaliQ64620.
SMRiQ64620. Positions 5-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64620. 2 interactions.
STRINGi10116.ENSRNOP00000021176.

PTM databases

PhosphoSiteiQ64620.

Proteomic databases

PRIDEiQ64620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021176; ENSRNOP00000021176; ENSRNOG00000015145.
GeneIDi171121.
KEGGirno:171121.
UCSCiRGD:708460. rat.

Organism-specific databases

CTDi5537.
RGDi708460. Ppp6c.

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00550000074961.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiQ64620.
KOiK15498.
OMAiENWAVSP.
OrthoDBiEOG74N5H2.
PhylomeDBiQ64620.
TreeFamiTF105563.

Miscellaneous databases

NextBioi621862.
PROiQ64620.

Gene expression databases

GenevestigatoriQ64620.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain."
    Becker W., Kentrup H., Klumpp S., Schultz J.E., Joost H.G.
    J. Biol. Chem. 269:22586-22592(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway.
    Tissue: Testis.

Entry informationi

Entry nameiPPP6_RAT
AccessioniPrimary (citable) accession number: Q64620
Secondary accession number(s): Q6AZ47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 15, 2008
Last modified: January 7, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.