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Q64620 (PPP6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 6 catalytic subunit
Short name=PP6C
EC=3.1.3.16
Alternative name(s):
Protein phosphatase V
Short name=PP-V
Gene names
Name:Ppp6c
Synonyms:Ppv
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalytic subunit of protein phosphatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7 By similarity.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Subunit structure

Protein phosphatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed by the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). Interacts with IGBP1, MAP3K7, NFKBIE, PPP6R1, PPP6R2 and PPP6R3 By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Highest levels found in spleen, brain and lung. Ref.1

Sequence similarities

Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.

Ontologies

Keywords
   Biological processCell cycle
   Cellular componentCytoplasm
   LigandIron
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

protein dephosphorylation

Inferred from mutant phenotype Ref.1. Source: RGD

   Cellular_componentcytosol

Inferred from electronic annotation. Source: Compara

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine phosphatase activity

Inferred from mutant phenotype Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 305304Serine/threonine-protein phosphatase 6 catalytic subunit
PRO_0000058879

Sites

Active site1141Proton donor By similarity
Metal binding531Iron By similarity
Metal binding551Iron By similarity
Metal binding811Iron By similarity
Metal binding811Manganese By similarity
Metal binding1131Manganese By similarity
Metal binding1631Manganese By similarity
Metal binding2371Manganese By similarity

Experimental info

Sequence conflict721P → L in CAA54453. Ref.1
Sequence conflict871Y → H in CAA54453. Ref.1
Sequence conflict911E → V in CAA54453. Ref.1
Sequence conflict2851F → N in CAA54453. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q64620 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: 662969DE1B2EF490

FASTA30535,159
        10         20         30         40         50         60 
MAPLDLDKYV EIARQCKYLP ENDLKRLCDY VCDLLLEESN VQPVSTPVTV CGDIHGQFYD 

        70         80         90        100        110        120 
LCELFRTGGQ VPDTNYIFMG DFVDRGYYSL ETFTYLLALK AKWPDRITLL RGNHESRQIT 

       130        140        150        160        170        180 
QVYGFYDECQ TKYGNANAWR YCTKVFDMLT VAALIDEQIL CVHGGLSPDI KTLDQIRTIE 

       190        200        210        220        230        240 
RNQEIPHKGA FCDLVWSDPE DVDTWAISPR GAGWLFGAKV TNEFVHINNL KLICRAHQLV 

       250        260        270        280        290        300 
HEGYKFMFDE KLVTVWSAPN YCYRCGNIAS IMVFKDVNTR EPKLFRAVPD SERVIPPRTT 


TPYFL

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain."
Becker W., Kentrup H., Klumpp S., Schultz J.E., Joost H.G.
J. Biol. Chem. 269:22586-22592(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain and Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X77236 mRNA. Translation: CAA54453.1.
BC078747 mRNA. Translation: AAH78747.1.
IPIIPI00214417.
PIRB55346.
RefSeqNP_598273.2. NM_133589.2.
UniGeneRn.9573.

3D structure databases

ProteinModelPortalQ64620.
SMRQ64620. Positions 5-276.
ModBaseSearch...

Protein-protein interaction databases

IntActQ64620. 2 interactions.
STRING10116.ENSRNOP00000021176.

PTM databases

PhosphoSiteQ64620.

Proteomic databases

PRIDEQ64620.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000021176; ENSRNOP00000021176; ENSRNOG00000015145.
GeneID171121.
KEGGrno:171121.
UCSCRGD:708460. rat.

Organism-specific databases

CTD5537.
RGD708460. Ppp6c.

Phylogenomic databases

eggNOGCOG0639.
GeneTreeENSGT00550000074961.
HOGENOMHOG000172696.
HOVERGENHBG000216.
InParanoidQ64620.
KOK15498.
OMANKYGNAN.
OrthoDBEOG495ZS5.

Gene expression databases

GenevestigatorQ64620.
GermOnlineENSRNOG00000015145. Rattus norvegicus.

Family and domain databases

InterProIPR004843. Metallo_PEstase_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio621862.

Entry information

Entry namePPP6_RAT
AccessionPrimary (citable) accession number: Q64620
Secondary accession number(s): Q6AZ47
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 15, 2008
Last modified: April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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