Reviewed, UniProtKB/Swiss-Prot Q64617 (KPCL_RAT)

Names and origin

Protein names

Recommended name:
    Protein kinase C eta type
    EC=2.7.11.13
Alternative name(s):
    nPKC-eta
    PKC-L

Gene names

Name:	Prkch
Synonyms:	Pkch

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation.
Domain	The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Ref.1 Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 683	683	Protein kinase C eta type		PRO_0000055707
Regions
	Domain	12 – 102	91	C2
	Domain	355 – 614	260	Protein kinase
	Domain	615 – 683	69	AGC-kinase C-terminal
	Zinc finger	171 – 222	52	Phorbol-ester/DAG-type 1
	Zinc finger	245 – 295	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	361 – 369	9	ATP By similarity
Sites
	Active site	479	1	Proton acceptor By similarity
	Binding site	384	1	ATP By similarity
Amino acid modifications
	Modified residue	57	1	Phosphothreonine By similarity
	Modified residue	317	1	Phosphoserine By similarity
	Modified residue	320	1	Phosphoserine By similarity
	Modified residue	513	1	Phosphothreonine Probable
	Modified residue	656	1	Phosphothreonine Probable
	Modified residue	675	1	Phosphoserine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q64617-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2F5B7078D1206510	FASTA	683	77,947
	10 20 30 40 50 60 MSSGTMKFNG YLRVRIGEAV GLQPTRWSLR HSLFKKGHQL LDPYLTVSVD QVRVGQTSTK 70 80 90 100 110 120 QKTNKPTYNE EFCTNVSDGG HLELAVFHET PLGYDHFVAN CTLQFQELLR TAGTSDTFEG 130 140 150 160 170 180 WVDLEPEGKV FVVITLTGSF TEATLQRDRI FKHFTRKRQR AMRRRVHQVN GHKFMATYLR 190 200 210 220 230 240 QPTYCSHCRE FIWGVFGKQG YQCQVCTCVV HKRCHHLIVT ACTCQNNINK VDAKIAEQRF 250 260 270 280 290 300 GINIPHKFNV HNYKVPTFCD HCGSLLWGIM RQGLQCKICK MNVHIRCQAN VAPNCGVNAV 310 320 330 340 350 360 ELAKTLAGMG LQPGNISPTS KLISRSTLRR QGKEGSKEGN GIGVNSSSRF GIDNFEFIRV 370 380 390 400 410 420 LGKGSFGKVM LARIKETGEL YAVKVLKKDV ILQDDDVECT MTEKRILSLA RNHPFLTQLF 430 440 450 460 470 480 CCFQTPDRLF FVMEFVNGGD LMFHIQKSRR FDEARARFYA AEIISALMFL HEKGIIYRDL 490 500 510 520 530 540 KLDNVLLDHE GHCKLADFGM CKEGICNGVT TATFCGTPDY IAPEILQEML YGPAVDWWAM 550 560 570 580 590 600 GVLLYEMLCG HAPFEAENED DLFEAILNDE VVYPTWLHED ATGILKSFMT KNPTMRLGSL 610 620 630 640 650 660 TQGGEHEILR HPFFKEIDWV QLNHRQLEPP FRPRIKSRED VSNFDPDFIK EEPVLTPIDE 670 680 GHLPMINQDE FRNFSYVSPE LQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical properties of rat protein kinase C-eta expressed in COS cells." Dekker L.V., Parker P.J., McIntyre P. FEBS Lett. 312:195-199(1992) [PubMed: 1426252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68400 mRNA. Translation: CAA48466.1. BC081782 mRNA. Translation: AAH81782.1.
IPI	IPI00214397.
PIR	S29478. I60246.
RefSeq	NP_112347.1.
UniGene	Rn.10398
3D structure databases
SMR	Q64617. Positions 117-266, 239-302, 326-557, 352-683.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64617.
PTM databases
PhosphoSite	Q64617.
Proteomic databases
PRIDE	Q64617.
Genome annotation databases
Ensembl	ENSRNOT00000006729; ENSRNOP00000006729; ENSRNOG00000004873; Rattus norvegicus. [Genome view]
GeneID	81749.
KEGG	rno:81749.
UCSC	NM_031085. rat.
Organism-specific databases
CTD	81749.
RGD	621888. Prkch.
Phylogenomic databases
eggNOG	roNOG06998.
HOVERGEN	Q64617.
InParanoid	Q64617.
OMA	RNFSFVS.
OrthoDB	EOG9CC6KS.
PhylomeDB	Q64617.
Enzyme and pathway databases
BRENDA	2.7.11.13. 248.
Gene expression databases
ArrayExpress	Q64617.
Genevestigator	Q64617.
GermOnline	ENSRNOG00000004873. Rattus norvegicus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR020454. DAG/PE_bd. IPR011009. Kinase-like_dom. IPR015745. PKC. IPR017892. Pkinase_C. IPR014376. Prot_kin_PKC_delta. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000551. PKC_delta. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615498.

Entry information

Entry name

KPCL_RAT

Accession

Primary (citable) accession number: Q64617

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)