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Q64612 (PTPRV_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor-type tyrosine-protein phosphatase V

Short name=R-PTP-V
EC=3.1.3.48
Alternative name(s):
Embryonic stem cell protein-tyrosine phosphatase
Short name=ES cell phosphatase
Osteotesticular protein-tyrosine phosphatase
Short name=OST-PTP
Gene names
Name:Ptprv
Synonyms:Esp
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1711 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function in signaling pathways during bone remodeling, as well as serve a broader role in cell interactions associated with differentiation in bone and testis. Associated with differentiation in bone and testis.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Bone and testis. In the latter, restricted to the basal portion of the seminiferous tubule.

Developmental stage

Up-regulated in differentiating cultures of primary osteoblasts and down-regulated in late stage mineralizing cultures. In testis, expression is highest between stages I and VII when maturing spermatids remain buried within the Sertoli epithelium.

Induction

By parathyroid hormone and cAMP analogs.

Post-translational modification

The cytoplasmic domain contains potential phosphorylation sites.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.

Contains 10 fibronectin type-III domains.

Contains 2 tyrosine-protein phosphatase domains.

Biophysicochemical properties

pH dependence:

Optimum pH is 5.6.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
Protein phosphatase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbone remodeling

Inferred from expression pattern Ref.1. Source: RGD

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Note: A presumed alternate transcript of 4.8-5.0 kilobases, which may lack PTP domains, is present in proliferating osteoblasts, but not detectable at other stages.
Isoform 1 (identifier: Q64612-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q64612-2)

The sequence of this isoform is not available.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 17111694Receptor-type tyrosine-protein phosphatase V
PRO_0000025467

Regions

Topological domain18 – 10741057Extracellular Potential
Transmembrane1075 – 109521Helical; Potential
Topological domain1096 – 1711616Cytoplasmic Potential
Domain37 – 12993Fibronectin type-III 1
Domain130 – 22293Fibronectin type-III 2
Domain218 – 30588Fibronectin type-III 3
Domain306 – 39186Fibronectin type-III 4
Domain393 – 47078Fibronectin type-III 5
Domain475 – 56995Fibronectin type-III 6
Domain565 – 65490Fibronectin type-III 7
Domain655 – 74995Fibronectin type-III 8
Domain744 – 83188Fibronectin type-III 9
Domain832 – 92695Fibronectin type-III 10
Domain1150 – 1409260Tyrosine-protein phosphatase 1
Domain1427 – 1696270Tyrosine-protein phosphatase 2
Region1350 – 13567Substrate binding By similarity

Sites

Active site13501Phosphocysteine intermediate By similarity
Binding site13161Substrate By similarity
Binding site13941Substrate By similarity

Amino acid modifications

Glycosylation421N-linked (GlcNAc...) Potential
Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation2391N-linked (GlcNAc...) Potential
Glycosylation2591N-linked (GlcNAc...) Potential
Glycosylation4311N-linked (GlcNAc...) Potential
Glycosylation5701N-linked (GlcNAc...) Potential
Glycosylation6201N-linked (GlcNAc...) Potential
Glycosylation6491N-linked (GlcNAc...) Potential
Glycosylation6631N-linked (GlcNAc...) Potential
Glycosylation7371N-linked (GlcNAc...) Potential
Glycosylation8511N-linked (GlcNAc...) Potential
Glycosylation8821N-linked (GlcNAc...) Potential
Glycosylation9701N-linked (GlcNAc...) Potential
Glycosylation9821N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0F04D2D1A47A18A0

FASTA1,711187,293
        10         20         30         40         50         60 
MRPLILLAAL LWLQGFLAED DACSSLEGSP DRQGGGPLLS VNVSSHGKST SLFLSWVAAE 

        70         80         90        100        110        120 
LGGFDYALSL RSVNSSGSPE GQQLQAHTNE SGFEFHGLVP GSRYQLKLTV LRPCWQNVTI 

       130        140        150        160        170        180 
TLTARTAPTV VRGLQLHSAG SPARLEASWS DAPGDQDSYQ LLLYHLESQT LACNVSVSPD 

       190        200        210        220        230        240 
TLSYSFGDLL PGTQYVLEVI TWAGSLHAKT SILQWTEPVP PDHLALRALG TSSLQAFWNS 

       250        260        270        280        290        300 
SEGATSFHLM LTDLLGGTNT TAVIRQGVST HTFLHLSPGT PHELKICASA GPHQIWGPSA 

       310        320        330        340        350        360 
TEWTYPSYPS DLVLTPLRNE LWASWKAGLG ARDGYVLKLS GPMESTSTLG PEECNAVFPG 

       370        380        390        400        410        420 
PLPPGHYTLQ LKVLAGPYDA WVEGSTWLAE SAALPREVPG ARLWLDGLEA SKQPGRRALL 

       430        440        450        460        470        480 
YSDDAPGSLG NISVPSGATH VIFCGLVPGA HYRVDIASST GDISQSISGY TSPLPPQSLE 

       490        500        510        520        530        540 
VISRSSPSDL TIAWGPAPGQ LEGYKVTWHQ DGSQRSPGDL VDLGPDTLSL TLKSLVPGSC 

       550        560        570        580        590        600 
YTVSAWAWAG NLDSDSQKIH SCTRPAPPTN LSLGFAHQPA ALKASWYHPP GGRDAFHLRL 

       610        620        630        640        650        660 
YRLRPLTLES EKVLPREAQN FSWAQLTAGC EFQVQLSTLW GSERSSSANA TGWTPPSAPT 

       670        680        690        700        710        720 
LVNVTSDAPT QLQVSWAHVP GGRSRYQVTL YQESTRTATS IMGPKEDGTS FLGLTPGTKY 

       730        740        750        760        770        780 
KVEVISWAGP LYTAAANVSA WTYPLIPNEL LVSMQAGSAV VNLAWPSGPL GQGACHAQLS 

       790        800        810        820        830        840 
DAGHLSWEQP LKLGQELFML RDLTPGHTIS MSVRCRAGPL QASTHLVVLS VEPGPVEDVL 

       850        860        870        880        890        900 
CHPEATYLAL NWTMPAGDVD VCLVVVERLV PGGGTHFVFQ VNTSGDALLL PNLMPTTSYR 

       910        920        930        940        950        960 
LSLTVLGRNS RWSRAVSLVC STSAEAWHPP ELAEPPQVEL GTGMGVTVMR GMFGKDDGQI 

       970        980        990       1000       1010       1020 
QWYGIIATIN MTLAQPSREA INYTWYDHYY RGCESFLALL FPNPFYPEPW AGPRSWTVPV 

      1030       1040       1050       1060       1070       1080 
GTEDCDNTQE ICNGRLKSGF QYRFSVVAFS RLNTPETILA FSAFSEPRAS ISLAIIPLTV 

      1090       1100       1110       1120       1130       1140 
MLGAVVGSIV IVCAVLCLLR WRCLKGPRSE KDGFSKELMP YNLWRTHRPI PIHSFRQSYE 

      1150       1160       1170       1180       1190       1200 
AKSAHAHQTF FQEFEELKEV GKDQPRLEAE HPDNIIKNRY PHVLPYDHSR VRLTQLPGEP 

      1210       1220       1230       1240       1250       1260 
HSDYINANFI PGYSHTQEII ATQGPLKKTL EDFWRLVWEQ QVHVIIMLTV GMENGRVLCE 

      1270       1280       1290       1300       1310       1320 
HYWPANSTPV THGHITIHLL AEEPEDEWTR REFQLQHGTE QKQRRVKQLQ FTTWPDHSVP 

      1330       1340       1350       1360       1370       1380 
EAPSSLLAFV ELVQEQVQAT QGKGPILVHC SAGVGRTGTF VALLRLLRQL EEEKVADVFN 

      1390       1400       1410       1420       1430       1440 
TVYILRLHRP LMIQTLSQYI FLHSCLLNKI LEGPPDSSDS GPISVMDFAQ ACAKRAANAN 

      1450       1460       1470       1480       1490       1500 
AGFLKEYKLL KQAIKDGTGS LLPPPDYNQN SIVSRRHSQE QFALVEECPE DSMLEASLFP 

      1510       1520       1530       1540       1550       1560 
GGPSGCDHVV LTGSAGPKEL WEMVWEHDAH VLVSLGLPDT KEKPPDIWPV EMQPIVTDMV 

      1570       1580       1590       1600       1610       1620 
TVHRVSESNT TTGWPSTLFR VIHGESGKER QVQCLQFPCS ESGCELPANT LLTFLDAVGQ 

      1630       1640       1650       1660       1670       1680 
CCFRGKSKKP GTLLSHSSKN TNQLGTFLAM EQLLQQAGTE RTVDVFNVAL KQSQACGLMT 

      1690       1700       1710 
PTLEQYIYLY NCLNSALLNG LPRAGKWPAP C 

« Hide

Isoform 2 (Sequence not available).

References

[1]"Identification of a hormonally regulated protein tyrosine phosphatase associated with bone and testicular differentiation."
Mauro L.J., Olmsted E.A., Skrobacz B.M., Mourey R.J., Davis A.R., Dixon J.E.
J. Biol. Chem. 269:30659-30667(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Osteosarcoma.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36884 mRNA. Translation: AAA63911.1.
PIRA55148.
RefSeqNP_149090.1. NM_033099.1. [Q64612-1]
UniGeneRn.10048.

3D structure databases

ProteinModelPortalQ64612.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000007597.

Proteomic databases

PaxDbQ64612.
PRIDEQ64612.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID64576.
KEGGrno:64576.
UCSCRGD:621123. rat. [Q64612-1]

Organism-specific databases

CTD13924.
RGD621123. Ptprv.

Phylogenomic databases

eggNOGCOG5599.
HOGENOMHOG000168222.
HOVERGENHBG053764.
InParanoidQ64612.
KOK18036.
PhylomeDBQ64612.

Gene expression databases

GenevestigatorQ64612.

Family and domain databases

Gene3D2.60.40.10. 3 hits.
3.90.190.10. 2 hits.
InterProIPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamPF00041. fn3. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00060. FN3. 8 hits.
SM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMSSF49265. SSF49265. 8 hits.
SSF52799. SSF52799. 2 hits.
PROSITEPS50853. FN3. 5 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio613540.

Entry information

Entry namePTPRV_RAT
AccessionPrimary (citable) accession number: Q64612
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families