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Q64604 (PTPRF_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Receptor-type tyrosine-protein phosphatase F
EC=3.1.3.48
Alternative name(s):
Leukocyte common antigen related
Short name=LAR
Gene names
Name:Ptprf
Synonyms:Lar
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length1898 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase). Ref.2

The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one By similarity. Ref.2

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with GRIP1 By similarity. Interacts with PPFIA1, PPFIA2 and PPFIA3 By similarity. Interacts with PTPRF By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.

Contains 8 fibronectin type-III domains.

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 2 tyrosine-protein phosphatase domains.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   LigandHeparin-binding
   Molecular functionHydrolase
Protein phosphatase
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhomophilic cell adhesion

Inferred from direct assay PubMed 12716943. Source: RGD

negative regulation of cell projection organization

Inferred from mutant phenotype PubMed 10699984. Source: RGD

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 16415345. Source: RGD

negative regulation of cytokine-mediated signaling pathway

Inferred from mutant phenotype PubMed 16415345. Source: RGD

negative regulation of epidermal growth factor receptor signaling pathway

Inferred from mutant phenotype PubMed 8557682. Source: RGD

negative regulation of insulin receptor signaling pathway

Inferred from mutant phenotype PubMed 8557682. Source: RGD

negative regulation of nerve growth factor receptor signaling pathway

Inferred from mutant phenotype PubMed 10699984. Source: RGD

positive regulation of dendrite morphogenesis

Inferred from mutant phenotype PubMed 15750591. Source: RGD

positive regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 10699984. Source: RGD

regulation of synapse structure and activity

Inferred from mutant phenotype PubMed 15750591. Source: RGD

   Cellular_componentendosome

Inferred from direct assay PubMed 16269466. Source: RGD

excitatory synapse

Inferred from direct assay PubMed 15750591. Source: RGD

growth cone

Inferred from direct assay PubMed 9604206. Source: RGD

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuronal cell body

Inferred from direct assay PubMed 9748473. Source: RGD

plasma membrane

Inferred from direct assay PubMed 10692429. Source: RGD

   Molecular_functionheparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

insulin receptor binding

Inferred from direct assay PubMed 7711057. Source: RGD

phosphate ion binding

Inferred from direct assay Ref.2. Source: RGD

transmembrane receptor protein tyrosine phosphatase activity

Inferred from direct assay PubMed 7711057. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 18981869Receptor-type tyrosine-protein phosphatase F
PRO_5000142152

Regions

Topological domain30 – 12541225Extracellular Potential
Transmembrane1255 – 127521Helical; Potential
Topological domain1276 – 1898623Cytoplasmic Potential
Domain33 – 12391Ig-like C2-type 1
Domain135 – 22490Ig-like C2-type 2
Domain232 – 31483Ig-like C2-type 3
Domain319 – 40789Fibronectin type-III 1
Domain413 – 50694Fibronectin type-III 2
Domain512 – 60291Fibronectin type-III 3
Domain606 – 70398Fibronectin type-III 4
Domain708 – 80699Fibronectin type-III 5
Domain808 – 90194Fibronectin type-III 6
Domain906 – 99994Fibronectin type-III 7
Domain1002 – 108685Fibronectin type-III 8
Domain1343 – 1598256Tyrosine-protein phosphatase 1
Domain1630 – 1889260Tyrosine-protein phosphatase 2
Region68 – 7710Heparin-binding By similarity
Region1539 – 15457Substrate binding By similarity

Sites

Active site15391Phosphocysteine intermediate By similarity
Active site18301Phosphocysteine intermediate By similarity
Binding site15071Substrate By similarity
Binding site15831Substrate By similarity

Amino acid modifications

Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation2501N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation7211N-linked (GlcNAc...) Potential
Glycosylation9411N-linked (GlcNAc...) Potential
Glycosylation9571N-linked (GlcNAc...) Potential
Disulfide bond54 ↔ 107 By similarity
Disulfide bond156 ↔ 207 By similarity
Disulfide bond253 ↔ 298 By similarity

Experimental info

Sequence conflict10731T → S in AAA41510. Ref.2
Sequence conflict14341I → T in AAA41510. Ref.2
Sequence conflict16391G → N in AAA41510. Ref.2
Sequence conflict1643 – 16442RA → HT in AAA41510. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q64604 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DF4D7E46F5896F4B

FASTA1,898211,494
        10         20         30         40         50         60 
MAPEPAPGRR MVPLVPALVM LGLMAGAHGD SKPVFVKVPE DQIGLSGGVA SFVCQATGEP 

        70         80         90        100        110        120 
KPRITWMKKG KKVSSQRFEV IEFDDGAGSV LRIQPLRVQR DEAIYECTAT NSLGEINTSA 

       130        140        150        160        170        180 
KLSVLEEDQL PSGFPTIDMG PQLKVVEKAR TATMLCAAGG NPDPEISWFK DFLPVDPASS 

       190        200        210        220        230        240 
NGRIKQLRSG ALQIESSEES DQGKYECVAT NSAGTRYSAP ANLYVRVRRV APRFSIPPSS 

       250        260        270        280        290        300 
QEVMPGGNVN LTCVAVGAPM PYVKWMMGAE ELTKEDEMPV GRNVLELSNV MRSANYTCVA 

       310        320        330        340        350        360 
ISSLGMIEAT AQVTVKALPK PPIDLVVTET TATSVTLTWD SGNTEPVSFY GIQYRAAGTD 

       370        380        390        400        410        420 
GPFQEVDGVA STRYSIGGLS PFSEYAFRVL AVNSIGRGPP SEAVRARTGE QAPSSPPRRV 

       430        440        450        460        470        480 
QARMLSASTM LVQWEPPEEP NGLVRGYRVY YTPDSRRPLS AWHKHNTDAG LLTTVGSLLP 

       490        500        510        520        530        540 
GITYSLRVLA FTAVGDGPPS PTIQVKTQQG VPAQPADFQA KAESDTRIQL SWLLPPQERI 

       550        560        570        580        590        600 
IKYELVYWAA EDEGQQHKVT FDPTSSYTLE DLKPDTLYHF QLAARSDLGV GVFTPTVEAC 

       610        620        630        640        650        660 
TAQSTPSAPP QKVTCVSTGS TTVRVSWVPP PADSRNGIIT QYSVAYEAVD GEDRKRHVVD 

       670        680        690        700        710        720 
GISREHSSWD LLGLEKWTEY RVWVRAHTDV GPGPESSPVL VRTDEDVPSG PPRKVEVEPL 

       730        740        750        760        770        780 
NSTAVHVSWK LPVPNKQHGQ IRGYQVTYVR LENGEPRGQP IIQDVMLAEA QETTISGLTP 

       790        800        810        820        830        840 
ETTYSITVAA YTTKGDGARS KPKVVTTTGA VPGRPTMMVS TTAMHTALLQ WHPPKELPGE 

       850        860        870        880        890        900 
LLGYRLQYRR ADEARPNTID FGKDDQHFTV TGLHKGATYI FRLAAKNRAG PGEEFEKEIT 

       910        920        930        940        950        960 
TPEDAPSGFP QNLRVTGLTT STTELAWDPP VLAERNGRIT NYTVVYRDIN SQHELQNVTG 

       970        980        990       1000       1010       1020 
DVHLTLLGLK PDTTYDIKVR AHTSKGAGPL SPSIQSRTMP MEQVFAKNFR VAAAMKTSVL 

      1030       1040       1050       1060       1070       1080 
LSWEVPDSYK SAVPFKILYN GQSVEVDGHS MRKLIADLQP NTEYSFVLMN RGTSAGGLQH 

      1090       1100       1110       1120       1130       1140 
LVSIRTAPDL LPQKPLPASA FIEDGRFSLS MPQVQDPSLV RWFYIVVVPI DRVGGNLLAP 

      1150       1160       1170       1180       1190       1200 
RWSTPEELEL DELLEAIEQG EEKQRRRRRQ AERLKPYVAA QVDELPETFT LGDKKNYRGF 

      1210       1220       1230       1240       1250       1260 
YNRPLSPDLS YQCFVLASLK EPMDQKRYAS SPYSDEIVVQ VTPAQQQEEP EMLWVTGPVL 

      1270       1280       1290       1300       1310       1320 
AVILIILIVI AILLFKRKRT HSPSSKDEQS IGLKDSLLAH SSDPVEMRRL NYQTPGMRDH 

      1330       1340       1350       1360       1370       1380 
PPIPITDLAD NIERLKANDG LKFSQEYESI DPGQQFTWEN SNSEVNKPKN RYANVIAYDH 

      1390       1400       1410       1420       1430       1440 
SRVLLTSIDG VPGSDYINAN YIDGYRKQNA YIATQGPLPE TMGDFWRMVW EQRIATVVMM 

      1450       1460       1470       1480       1490       1500 
TRLEEKSRVK CDQYWPARGT ETYGLIQVTL VDTVELATYT MRTFALHKSG SSEKRELRQF 

      1510       1520       1530       1540       1550       1560 
QFMAWPDHGV PEYPTPILAF LRRVKACNPL DAGPMVVHCS AGVGRTGCFI VIDAMLERMK 

      1570       1580       1590       1600       1610       1620 
HEKTVDIYGH VTCMRSQRNY MVQTEDQYVF IHEALLEAAM CGHTEVLARN LYAHIQKLGQ 

      1630       1640       1650       1660       1670       1680 
VPPGESVTAM ELEFKLLAGS KARASRFISA NLPCNKFKNR LVNIMPYELT RVCLQPIRGV 

      1690       1700       1710       1720       1730       1740 
EGSDYINASF LDGYRQQKAY IATQGPLAES TEDFWRMLWE HNSTIIVMLT KLREMGREKC 

      1750       1760       1770       1780       1790       1800 
HQYWPAERSA RYQYFVVDPM AEYNMPQYIL REFKVTDARD GQSRTIRQFQ FTDWPEQGVP 

      1810       1820       1830       1840       1850       1860 
KTGEGFIDFI GQVHKTKEQF GQDGPITVHC SAGVGRTGVF ITLSIVLERM RYEGVVDMFQ 

      1870       1880       1890 
TVKTLRTQRP AMVQTEDQYQ LCYRAALEYL GSFDHYAT

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References

[1]"Molecular cloning and expression of a unique receptor-like protein-tyrosine-phosphatase in the leucocyte-common-antigen-related phosphate family."
Zhang W.-R., Hashimoto N., Ahmad F., Ding W., Goldstein B.J.
Biochem. J. 302:39-47(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Cloning, bacterial expression, purification, and characterization of the cytoplasmic domain of rat LAR, a receptor-like protein tyrosine phosphatase."
Pot D.A., Woodford T.A., Remboutsika E., Haun R.S., Dixon J.E.
J. Biol. Chem. 266:19688-19696(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1035-1898, FUNCTION.
Tissue: Hypothalamus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L11586 mRNA. Translation: AAC37655.1.
M60103 mRNA. Translation: AAA41510.1.
IPIIPI00231988.
PIRS46216.
RefSeqNP_062122.1. NM_019249.1.
UniGeneRn.11386.

3D structure databases

HSSPHSSP built from PDB template 1LAR based on UniProtKB P10586.
ProteinModelPortalQ64604.
SMRQ64604. Positions 319-415, 596-719, 814-904, 909-1001, 1325-1893.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000027271.

Proteomic databases

PaxDbQ64604.
PRIDEQ64604.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID360406.
KEGGrno:360406.
UCSCRGD:3453. rat.

Organism-specific databases

CTD5792.
RGD3453. Ptprf.

Phylogenomic databases

eggNOGCOG5599.
HOGENOMHOG000010250.
InParanoidQ64604.
KOK05695.
OrthoDBEOG4BG8V3.

Gene expression databases

ArrayExpressQ64604.
GenevestigatorQ64604.

Family and domain databases

Gene3D2.60.40.10. 10 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamPF00041. fn3. 7 hits.
PF07679. I-set. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00060. FN3. 8 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 2 hits.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMSSF49265. FN_III-like. 8 hits.
PROSITEPS50853. FN3. 8 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

BindingDBQ64604.
ChEMBLCHEMBL2834.
NextBio672799.

Entry information

Entry namePTPRF_RAT
AccessionPrimary (citable) accession number: Q64604
Secondary accession number(s): Q4JFJ1 expand/collapse secondary AC list , Q63294, Q63295, Q63296
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents