Reviewed, UniProtKB/Swiss-Prot Q64591 (DECR_RAT)

Names and origin

Protein names

Recommended name:
    2,4-dienoyl-CoA reductase, mitochondrial
    EC=1.3.1.34
Alternative name(s):
    2,4-dienoyl-CoA reductase [NADPH]
      Short name=4-enoyl-CoA reductase [NADPH]

Gene names

Name:	Decr1
Synonyms:	Decr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.
Catalytic activity	Trans-2,3-didehydroacyl-CoA + NADP+ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,4-dienoyl-CoA reductase (NADPH) activity Ref.1 Traceable author statement. Source: RGD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion Ref.1
	Chain	35 – 335	301	2,4-dienoyl-CoA reductase, mitochondrial		PRO_0000031967
Regions
	Nucleotide binding	62 – 94	33	NADP By similarity
Sites
	Active site	199	1	Proton acceptor By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	214	1	NADP By similarity
Amino acid modifications
	Modified residue	106	1	N6-acetyllysine By similarity
	Modified residue	110	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	11	1	G → R in AAH59120. Ref.2
	Sequence conflict	23	1	R → K in BAA00446. Ref.1
	Sequence conflict	79	1	L → R in BAA00446. Ref.1
	Sequence conflict	162	1	K → R in BAA00446. Ref.1
	Sequence conflict	190	1	A → V in BAA00446. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q64591-1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: C4386C65E49A4D2F	FASTA	335	36,133
	10 20 30 40 50 60 MALLARAFFA GVSRLPCDPG PQRFFSFGTK TLYQSIDAPQ SKFFPPILKP MLPPNAFQGK 70 80 90 100 110 120 VAFITGGGTG LGKAMTTFLS SLGAQCVIAS RNIDVLKATA EEITSKTGNK VYAIRCDVRD 130 140 150 160 170 180 PDMVHNTVLE LIKVAGHPDV VINNAAGNFI SPSERLSPNG WKTITDIVLN GTAYVTIEIG 190 200 210 220 230 240 KQLIKAQKGA AFLAITTIYA ESGSGFVMPS SSAKSGVEAM NKSLAAEWGR YGMRFNIIQP 250 260 270 280 290 300 GPIKTKGAFS RLDPTGKFEK DMIERIPCGR LGTVEELANL ATFLCSDYAS WINGAVIRFD 310 320 330 GGEEVFLSGE FNSLKKVTKE EWDVIEGLIR KTKGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of rat liver 2,4-dienoyl-CoA reductase." Hirose A., Kamijo K., Osumi T., Hashimoto T., Mizugaki M. Biochim. Biophys. Acta 1049:346-349(1990) [PubMed: 2383590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-53. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D00569 mRNA. Translation: BAA00446.1. BC059120 mRNA. Translation: AAH59120.1.
IPI	IPI00213659.
PIR	S11021.
RefSeq	NP_476545.1.
UniGene	Rn.2854
3D structure databases
HSSP	HSSP built from PDB template 1LX6 based on UniProtKB P29132.
SMR	Q64591. Positions 37-328.
ModBase	Search...
Proteomic databases
PRIDE	Q64591.
Genome annotation databases
Ensembl	ENSRNOG00000008236. Rattus norvegicus. [Contig view]
GeneID	117543.
KEGG	rno:117543.
Organism-specific databases
RGD	70999. Decr1.
Phylogenomic databases
HOVERGEN	Q64591.
Enzyme and pathway databases
BRENDA	1.3.1.34. 248.
Gene expression databases
ArrayExpress	Q64591.
GermOnline	ENSRNOG00000008236. Rattus norvegicus.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620365.

Entry information

Entry name

DECR_RAT

Accession

Primary (citable) accession number: Q64591
Secondary accession number(s): Q6PCV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)