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Q64578

- AT2A1_RAT

UniProt

Q64578 - AT2A1_RAT

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Protein
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Gene
Atp2a1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction By similarity.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi304 – 3041Calcium 2; via carbonyl oxygen By similarity
Metal bindingi305 – 3051Calcium 2; via carbonyl oxygen By similarity
Metal bindingi307 – 3071Calcium 2; via carbonyl oxygen By similarity
Metal bindingi309 – 3091Calcium 2 By similarity
Active sitei351 – 35114-aspartylphosphate intermediate By similarity
Metal bindingi703 – 7031Magnesium By similarity
Metal bindingi707 – 7071Magnesium By similarity
Metal bindingi768 – 7681Calcium 1 By similarity
Metal bindingi771 – 7711Calcium 1 By similarity
Metal bindingi796 – 7961Calcium 2 By similarity
Metal bindingi799 – 7991Calcium 1 By similarity
Metal bindingi800 – 8001Calcium 1 By similarity
Metal bindingi800 – 8001Calcium 2 By similarity
Metal bindingi908 – 9081Calcium 1 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. calcium ion binding Source: UniProtKB
  3. calcium-transporting ATPase activity Source: UniProtKB
  4. nucleotide-sugar transmembrane transporter activity Source: RGD
  5. protein binding Source: UniProtKB

GO - Biological processi

  1. ATP catabolic process Source: UniProtKB
  2. calcium ion transmembrane transport Source: GOC
  3. calcium ion transport Source: UniProtKB
  4. carbohydrate derivative transport Source: GOC
  5. negative regulation of striated muscle contraction Source: UniProtKB
  6. nucleotide transmembrane transport Source: GOC
  7. positive regulation of fast-twitch skeletal muscle fiber contraction Source: UniProtKB
  8. regulation of muscle contraction Source: RGD
  9. regulation of striated muscle contraction Source: UniProtKB
  10. response to peptide hormone Source: UniProtKB
  11. sarcoplasmic reticulum calcium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (EC:3.6.3.8)
Short name:
SERCA1
Short name:
SR Ca(2+)-ATPase 1
Alternative name(s):
Calcium pump 1
Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Gene namesi
Name:Atp2a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621293. Atp2a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848Cytoplasmic By similarity
Add
BLAST
Transmembranei49 – 6921Helical; Name=1; By similarity
Add
BLAST
Topological domaini70 – 8920Lumenal By similarity
Add
BLAST
Transmembranei90 – 11021Helical; Name=2; By similarity
Add
BLAST
Topological domaini111 – 253143Cytoplasmic By similarity
Add
BLAST
Transmembranei254 – 27320Helical; Name=3; By similarity
Add
BLAST
Topological domaini274 – 29522Lumenal By similarity
Add
BLAST
Transmembranei296 – 31318Helical; Name=4; By similarity
Add
BLAST
Topological domaini314 – 757444Cytoplasmic By similarity
Add
BLAST
Transmembranei758 – 77720Helical; Name=5; By similarity
Add
BLAST
Topological domaini778 – 78710Lumenal By similarity
Transmembranei788 – 80821Helical; Name=6; By similarity
Add
BLAST
Topological domaini809 – 82820Cytoplasmic By similarity
Add
BLAST
Transmembranei829 – 85123Helical; Name=7; By similarity
Add
BLAST
Topological domaini852 – 89746Lumenal By similarity
Add
BLAST
Transmembranei898 – 91720Helical; Name=8; By similarity
Add
BLAST
Topological domaini918 – 93013Cytoplasmic By similarity
Add
BLAST
Transmembranei931 – 94919Helical; Name=9; By similarity
Add
BLAST
Topological domaini950 – 96415Lumenal By similarity
Add
BLAST
Transmembranei965 – 98521Helical; Name=10; By similarity
Add
BLAST
Topological domaini986 – 9949Cytoplasmic By similarity

GO - Cellular componenti

  1. H zone Source: UniProtKB
  2. I band Source: UniProtKB
  3. endoplasmic reticulum Source: UniProtKB
  4. endoplasmic reticulum membrane Source: UniProtKB
  5. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  6. integral component of membrane Source: UniProtKB-KW
  7. intracellular membrane-bounded organelle Source: RGD
  8. membrane Source: UniProtKB
  9. perinuclear region of cytoplasm Source: UniProtKB
  10. sarcoplasmic reticulum Source: UniProtKB
  11. sarcoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 994994Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
PRO_0000046190Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi876 ↔ 888 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ64578.

PTM databases

PhosphoSiteiQ64578.

Expressioni

Tissue specificityi

Skeletal muscle, fast twitch muscle (type II) fibers.

Inductioni

Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression.

Gene expression databases

GenevestigatoriQ64578.

Interactioni

Subunit structurei

Associated with sarcolipin (SLN) and phospholamban (PLN) By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ64578.
SMRiQ64578. Positions 1-994.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni370 – 40031Interacts with phospholamban 1 By similarity
Add
BLAST
Regioni788 – 80821Interacts with phospholamban 2 By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG105648.
KOiK05853.
PhylomeDBiQ64578.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64578-1 [UniParc]FASTAAdd to Basket

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MEAAHSKSTE ECLSYFGVSE TTGLTPDQVK RHLEKYGPNE LPAEEGKSLW    50
ELVVEQFEDL LVRILLLAAC ISFVLAWFEE GEETVTAFVE PFVILLILIA 100
NAIVGVWQER NAENAIEALK EYEPEMGKVY RADRKSVQRI KARDIVPGDI 150
VEVAVGDKVP ADIRILSIKS TTLRVDQSIL TGESVSVIKH TDPVPDPRAV 200
NQDKKNMLFS GTNIAAGKAV GIVATTGVST EIGKIRDQMA ATEQDKTPLQ 250
QKLDEFGEQL SKVISLICVA VWLINIGHFN DPVHGGSWFR GAIYYFKIAV 300
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS 350
DKTGTLTTNQ MSVCKMFIID KVDGDICSLN EFSITGSTYA PEGEVLKNDK 400
PVRAGQYDGL VELATICALC NDSSLDFNET KGVYEKVGEA TETALTTLVE 450
KMNVFNTEVR SLSKVERANA CNSVIRQLMK KEFTLEFSRD RKSMSVYCSP 500
AKSSRAAVGN KMFVKGAPEG VIDRCNYVRV GTTRVPLTGP VKEKIMSVIK 550
EWGTGRDTLR CLALATRDTP PKREEMVLDD SAKFMEYEMD LTFVGVVGML 600
DPPRKEVTGS IQLCRDAGIR VIMITGDNKG TAIAICRRIG IFSENEEVAD 650
RAYTGREFDD LPLAEQREAC RRACCFARVE PSHKSKIVEY LQSYDEITAM 700
TGDGVNDAPA LKKAEIGIAM GSGTAVAKTA SEMVLADDNF STIVAAVEEG 750
RAIYNNMKQF IRYLISSNVG EVVCIFLTAA LGLPEALIPV QLLWVNLVTD 800
GLPATALGFN PPDLDIMDRP PRSPKEPLIS GWLFFRYMAI GGYVGAATVG 850
AAAWWFLYAE DGPHVSYHQL THFMQCTEHN PEFDGLDCEV FEAPEPMTMA 900
LSVLVTIEMC NALNSLSENQ SLLRMPPWVN IWLLGSICLS MSLHFLILYV 950
DPLPMIFKLR ALDFTQWLMV LKISLPVIGL DELLKFIARN YLEG 994
Length:994
Mass (Da):109,409
Last modified:November 1, 1996 - v1
Checksum:i899F91AD8038A47A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M99223 mRNA. Translation: AAA40991.1.
AF091853 Genomic DNA. Translation: AAD17802.1.
AF091852 Genomic DNA. Translation: AAD17801.1.
PIRiA48849.
RefSeqiNP_478120.1. NM_058213.1.
UniGeneiRn.217139.

Genome annotation databases

GeneIDi116601.
KEGGirno:116601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M99223 mRNA. Translation: AAA40991.1 .
AF091853 Genomic DNA. Translation: AAD17802.1 .
AF091852 Genomic DNA. Translation: AAD17801.1 .
PIRi A48849.
RefSeqi NP_478120.1. NM_058213.1.
UniGenei Rn.217139.

3D structure databases

ProteinModelPortali Q64578.
SMRi Q64578. Positions 1-994.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q64578.

Proteomic databases

PRIDEi Q64578.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 116601.
KEGGi rno:116601.

Organism-specific databases

CTDi 487.
RGDi 621293. Atp2a1.

Phylogenomic databases

HOVERGENi HBG105648.
KOi K05853.
PhylomeDBi Q64578.

Miscellaneous databases

NextBioi 619311.
PROi Q64578.

Gene expression databases

Genevestigatori Q64578.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
PR00120. HATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and quantification of sarcoplasmic reticulum Ca(2+)-ATPase isoforms in rat muscles."
    Wu K.D., Lytton J.
    Am. J. Physiol. 264:C333-C341(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD Charles River.
    Tissue: Skeletal muscle.
  2. "Unloading induces transcriptional activation of the sarco(endo)plasmic reticulum Ca2+-ATPase 1 gene in muscle."
    Peters D.G., Mitchell-Felton H., Kandarian S.C.
    Am. J. Physiol. 276:C1218-C1225(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 955-994.
    Strain: Sprague-Dawley.
    Tissue: Kidney.

Entry informationi

Entry nameiAT2A1_RAT
AccessioniPrimary (citable) accession number: Q64578
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi