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Protein

Liver carboxylesterase 4

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes omicron-nitrophenyl acetate and alpha-naphthyl acetate. It also hydrolyzes acetanilide and, distinctively, palmitoyl-CoA.

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei353 – 3531Charge relay systemBy similarity
Active sitei466 – 4661Charge relay systemBy similarity

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: GO_Central

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BRENDAi3.1.1.1. 5301.

Protein family/group databases

MEROPSiS09.965.

Names & Taxonomyi

Protein namesi
Recommended name:
Liver carboxylesterase 4 (EC:3.1.1.1)
Alternative name(s):
Carboxyesterase ES-4
Kidney microsomal carboxylesterase
Microsomal palmitoyl-CoA hydrolase
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1642419. LOC100125372.

Subcellular locationi

Endoplasmic reticulum lumen
Note: Microsomal membrane, lumen of endoplasmic reticulum.

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 561543Liver carboxylesterase 4PRO_0000008581Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi87 ↔ 116By similarity
Disulfide bondi273 ↔ 284By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ64573.
PRIDEiQ64573.

Expressioni

Gene expression databases

GenevestigatoriQ64573.

Structurei

3D structure databases

SMRiQ64573. Positions 21-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi558 – 5614Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2272.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiQ64573.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64573-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCLSFLFLVS LATCVVYGNP SSPPVVDTTK GKVLGKYVSL EGVTQSVAVF
60 70 80 90 100
LGVPFAKPPL GSLRFAPPQP AEPWSFVKNT TTYPPMCSQD AAKGQRMNDL
110 120 130 140 150
LTNRKEKIHL EFSEDCLYLN IYTPADFTKN SRLPVMVWIH GGGMTLGGAS
160 170 180 190 200
TYDGRVLSAY ENVVVVAIQY RLGIWGFFST GDEHSRGNWG HLDQVAALHW
210 220 230 240 250
VQDNIANFGG DPGSVTIFGE SAGGFSVSVL VLSPLTKNLF HRAISESGVV
260 270 280 290 300
FLPGLLTKDV RPAAKQIADM AGCETTTSAI IVHCLRQKTE EELLEIMKKM
310 320 330 340 350
NLIKLSSQRD NKESYHFLST VVDNVVLPKD PKEILAEKNF NTVPYIVGIN
360 370 380 390 400
KQECGWLLPT MMGFVPADVE LDKKMAITLL EKFASLYGIP EDIIPVAIEK
410 420 430 440 450
YRKGSDDSIK IRDGILAFIG DVSFSIPSVM VSRDHRDAGA PTYMYEYQYY
460 470 480 490 500
PSFSSPQRPK HVVGDHADDL YSVFGAPILR DGASEEEIKL SKMVMKFWAN
510 520 530 540 550
FARNGNPNGR GLPHWPQYDQ KEEYLQIGAT TQQSQRLKAE EVAFWTQLLA
560
KRQPQPHHNE L
Length:561
Mass (Da):62,308
Last modified:July 27, 2009 - v2
Checksum:iEDF48D0F42F2AA1A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71F → I in CAA57419 (PubMed:8611161).Curated
Sequence conflicti60 – 601L → P in AAA64638 (PubMed:7961958).Curated
Sequence conflicti213 – 2131G → A in AAA64638 (PubMed:7961958).Curated
Sequence conflicti253 – 2531P → T in AAA64638 (PubMed:7961958).Curated
Sequence conflicti310 – 3112DN → IT in AAA64638 (PubMed:7961958).Curated
Sequence conflicti342 – 3421T → N in AAA64638 (PubMed:7961958).Curated
Sequence conflicti425 – 4262SI → FY in AAA64638 (PubMed:7961958).Curated
Sequence conflicti509 – 5091G → A in AAA64638 (PubMed:7961958).Curated
Sequence conflicti553 – 5531Q → E in AAA64638 (PubMed:7961958).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81825 mRNA. Translation: CAA57419.1.
U10697 mRNA. Translation: AAA64638.1.
BC128711 mRNA. Translation: AAI28712.1.
PIRiS62788.
RefSeqiNP_001096829.2. NM_001103359.2.
UniGeneiRn.82692.

Genome annotation databases

GeneIDi100125372.
KEGGirno:100125372.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81825 mRNA. Translation: CAA57419.1.
U10697 mRNA. Translation: AAA64638.1.
BC128711 mRNA. Translation: AAI28712.1.
PIRiS62788.
RefSeqiNP_001096829.2. NM_001103359.2.
UniGeneiRn.82692.

3D structure databases

SMRiQ64573. Positions 21-551.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS09.965.

Proteomic databases

PaxDbiQ64573.
PRIDEiQ64573.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100125372.
KEGGirno:100125372.

Organism-specific databases

CTDi234564.
RGDi1642419. LOC100125372.

Phylogenomic databases

eggNOGiCOG2272.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiQ64573.

Enzyme and pathway databases

BRENDAi3.1.1.1. 5301.

Miscellaneous databases

NextBioi744456.

Gene expression databases

GenevestigatoriQ64573.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of rat liver carboxylesterase ES-4 (microsomal palmitoyl-CoA hydrolase)."
    Robbi M., van Schaftingen E., Beaufay H.
    Biochem. J. 313:821-826(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Rat kidney carboxylesterase. Cloning, sequencing, cellular localization, and relationship to rat liver hydrolase."
    Yan B., Yang D., Brady M., Parkinson A.
    J. Biol. Chem. 269:29688-29696(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiEST4_RAT
AccessioniPrimary (citable) accession number: Q64573
Secondary accession number(s): A2VCW1, Q62679
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: July 27, 2009
Last modified: March 31, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.