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Q64573 (EST4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Liver carboxylesterase 4

EC=3.1.1.1
Alternative name(s):
Carboxyesterase ES-4
Kidney microsomal carboxylesterase
Microsomal palmitoyl-CoA hydrolase
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes omicron-nitrophenyl acetate and alpha-naphthyl acetate. It also hydrolyzes acetanilide and, distinctively, palmitoyl-CoA.

Catalytic activity

A carboxylic ester + H2O = an alcohol + a carboxylate.

Subcellular location

Endoplasmic reticulum lumen. Note: Microsomal membrane, lumen of endoplasmic reticulum.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
   DomainSignal
   Molecular functionHydrolase
Serine esterase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarboxylic ester hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 By similarity
Chain19 – 561543Liver carboxylesterase 4
PRO_0000008581

Regions

Motif558 – 5614Prevents secretion from ER Potential

Sites

Active site2211Acyl-ester intermediate By similarity
Active site3531Charge relay system By similarity
Active site4661Charge relay system By similarity

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Disulfide bond87 ↔ 116 By similarity
Disulfide bond273 ↔ 284 By similarity

Experimental info

Sequence conflict71F → I in CAA57419. Ref.1
Sequence conflict601L → P in AAA64638. Ref.2
Sequence conflict2131G → A in AAA64638. Ref.2
Sequence conflict2531P → T in AAA64638. Ref.2
Sequence conflict310 – 3112DN → IT in AAA64638. Ref.2
Sequence conflict3421T → N in AAA64638. Ref.2
Sequence conflict425 – 4262SI → FY in AAA64638. Ref.2
Sequence conflict5091G → A in AAA64638. Ref.2
Sequence conflict5531Q → E in AAA64638. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q64573 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: EDF48D0F42F2AA1A

FASTA56162,308
        10         20         30         40         50         60 
MCLSFLFLVS LATCVVYGNP SSPPVVDTTK GKVLGKYVSL EGVTQSVAVF LGVPFAKPPL 

        70         80         90        100        110        120 
GSLRFAPPQP AEPWSFVKNT TTYPPMCSQD AAKGQRMNDL LTNRKEKIHL EFSEDCLYLN 

       130        140        150        160        170        180 
IYTPADFTKN SRLPVMVWIH GGGMTLGGAS TYDGRVLSAY ENVVVVAIQY RLGIWGFFST 

       190        200        210        220        230        240 
GDEHSRGNWG HLDQVAALHW VQDNIANFGG DPGSVTIFGE SAGGFSVSVL VLSPLTKNLF 

       250        260        270        280        290        300 
HRAISESGVV FLPGLLTKDV RPAAKQIADM AGCETTTSAI IVHCLRQKTE EELLEIMKKM 

       310        320        330        340        350        360 
NLIKLSSQRD NKESYHFLST VVDNVVLPKD PKEILAEKNF NTVPYIVGIN KQECGWLLPT 

       370        380        390        400        410        420 
MMGFVPADVE LDKKMAITLL EKFASLYGIP EDIIPVAIEK YRKGSDDSIK IRDGILAFIG 

       430        440        450        460        470        480 
DVSFSIPSVM VSRDHRDAGA PTYMYEYQYY PSFSSPQRPK HVVGDHADDL YSVFGAPILR 

       490        500        510        520        530        540 
DGASEEEIKL SKMVMKFWAN FARNGNPNGR GLPHWPQYDQ KEEYLQIGAT TQQSQRLKAE 

       550        560 
EVAFWTQLLA KRQPQPHHNE L 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of rat liver carboxylesterase ES-4 (microsomal palmitoyl-CoA hydrolase)."
Robbi M., van Schaftingen E., Beaufay H.
Biochem. J. 313:821-826(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Rat kidney carboxylesterase. Cloning, sequencing, cellular localization, and relationship to rat liver hydrolase."
Yan B., Yang D., Brady M., Parkinson A.
J. Biol. Chem. 269:29688-29696(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Kidney.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X81825 mRNA. Translation: CAA57419.1.
U10697 mRNA. Translation: AAA64638.1.
BC128711 mRNA. Translation: AAI28712.1.
PIRS62788.
RefSeqNP_001096829.2. NM_001103359.2.
UniGeneRn.82692.

3D structure databases

ProteinModelPortalQ64573.
SMRQ64573. Positions 21-551.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS09.965.

Proteomic databases

PaxDbQ64573.
PRIDEQ64573.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100125372.
KEGGrno:100125372.

Organism-specific databases

CTD234564.
RGD1642419. LOC100125372.

Phylogenomic databases

eggNOGCOG2272.
HOGENOMHOG000091866.
HOVERGENHBG008839.

Enzyme and pathway databases

BRENDA3.1.1.1. 5301.

Gene expression databases

GenevestigatorQ64573.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio744456.

Entry information

Entry nameEST4_RAT
AccessionPrimary (citable) accession number: Q64573
Secondary accession number(s): A2VCW1, Q62679
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 28, 2009
Last modified: June 11, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families