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Q64573

- EST4_RAT

UniProt

Q64573 - EST4_RAT

Protein

Liver carboxylesterase 4

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes omicron-nitrophenyl acetate and alpha-naphthyl acetate. It also hydrolyzes acetanilide and, distinctively, palmitoyl-CoA.

    Catalytic activityi

    A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei221 – 2211Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei353 – 3531Charge relay systemBy similarity
    Active sitei466 – 4661Charge relay systemBy similarity

    GO - Molecular functioni

    1. carboxylic ester hydrolase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Enzyme and pathway databases

    BRENDAi3.1.1.1. 5301.

    Protein family/group databases

    MEROPSiS09.965.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Liver carboxylesterase 4 (EC:3.1.1.1)
    Alternative name(s):
    Carboxyesterase ES-4
    Kidney microsomal carboxylesterase
    Microsomal palmitoyl-CoA hydrolase
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1642419. LOC100125372.

    Subcellular locationi

    Endoplasmic reticulum lumen
    Note: Microsomal membrane, lumen of endoplasmic reticulum.

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818By similarityAdd
    BLAST
    Chaini19 – 561543Liver carboxylesterase 4PRO_0000008581Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi87 ↔ 116By similarity
    Disulfide bondi273 ↔ 284By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ64573.
    PRIDEiQ64573.

    Expressioni

    Gene expression databases

    GenevestigatoriQ64573.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64573.
    SMRiQ64573. Positions 21-551.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi558 – 5614Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2272.
    HOGENOMiHOG000091866.
    HOVERGENiHBG008839.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    [Graphical view]
    PfamiPF00135. COesterase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q64573-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCLSFLFLVS LATCVVYGNP SSPPVVDTTK GKVLGKYVSL EGVTQSVAVF    50
    LGVPFAKPPL GSLRFAPPQP AEPWSFVKNT TTYPPMCSQD AAKGQRMNDL 100
    LTNRKEKIHL EFSEDCLYLN IYTPADFTKN SRLPVMVWIH GGGMTLGGAS 150
    TYDGRVLSAY ENVVVVAIQY RLGIWGFFST GDEHSRGNWG HLDQVAALHW 200
    VQDNIANFGG DPGSVTIFGE SAGGFSVSVL VLSPLTKNLF HRAISESGVV 250
    FLPGLLTKDV RPAAKQIADM AGCETTTSAI IVHCLRQKTE EELLEIMKKM 300
    NLIKLSSQRD NKESYHFLST VVDNVVLPKD PKEILAEKNF NTVPYIVGIN 350
    KQECGWLLPT MMGFVPADVE LDKKMAITLL EKFASLYGIP EDIIPVAIEK 400
    YRKGSDDSIK IRDGILAFIG DVSFSIPSVM VSRDHRDAGA PTYMYEYQYY 450
    PSFSSPQRPK HVVGDHADDL YSVFGAPILR DGASEEEIKL SKMVMKFWAN 500
    FARNGNPNGR GLPHWPQYDQ KEEYLQIGAT TQQSQRLKAE EVAFWTQLLA 550
    KRQPQPHHNE L 561
    Length:561
    Mass (Da):62,308
    Last modified:July 28, 2009 - v2
    Checksum:iEDF48D0F42F2AA1A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71F → I in CAA57419. (PubMed:8611161)Curated
    Sequence conflicti60 – 601L → P in AAA64638. (PubMed:7961958)Curated
    Sequence conflicti213 – 2131G → A in AAA64638. (PubMed:7961958)Curated
    Sequence conflicti253 – 2531P → T in AAA64638. (PubMed:7961958)Curated
    Sequence conflicti310 – 3112DN → IT in AAA64638. (PubMed:7961958)Curated
    Sequence conflicti342 – 3421T → N in AAA64638. (PubMed:7961958)Curated
    Sequence conflicti425 – 4262SI → FY in AAA64638. (PubMed:7961958)Curated
    Sequence conflicti509 – 5091G → A in AAA64638. (PubMed:7961958)Curated
    Sequence conflicti553 – 5531Q → E in AAA64638. (PubMed:7961958)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81825 mRNA. Translation: CAA57419.1.
    U10697 mRNA. Translation: AAA64638.1.
    BC128711 mRNA. Translation: AAI28712.1.
    PIRiS62788.
    RefSeqiNP_001096829.2. NM_001103359.2.
    UniGeneiRn.82692.

    Genome annotation databases

    GeneIDi100125372.
    KEGGirno:100125372.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81825 mRNA. Translation: CAA57419.1 .
    U10697 mRNA. Translation: AAA64638.1 .
    BC128711 mRNA. Translation: AAI28712.1 .
    PIRi S62788.
    RefSeqi NP_001096829.2. NM_001103359.2.
    UniGenei Rn.82692.

    3D structure databases

    ProteinModelPortali Q64573.
    SMRi Q64573. Positions 21-551.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S09.965.

    Proteomic databases

    PaxDbi Q64573.
    PRIDEi Q64573.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100125372.
    KEGGi rno:100125372.

    Organism-specific databases

    CTDi 234564.
    RGDi 1642419. LOC100125372.

    Phylogenomic databases

    eggNOGi COG2272.
    HOGENOMi HOG000091866.
    HOVERGENi HBG008839.

    Enzyme and pathway databases

    BRENDAi 3.1.1.1. 5301.

    Miscellaneous databases

    NextBioi 744456.

    Gene expression databases

    Genevestigatori Q64573.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    [Graphical view ]
    Pfami PF00135. COesterase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of rat liver carboxylesterase ES-4 (microsomal palmitoyl-CoA hydrolase)."
      Robbi M., van Schaftingen E., Beaufay H.
      Biochem. J. 313:821-826(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "Rat kidney carboxylesterase. Cloning, sequencing, cellular localization, and relationship to rat liver hydrolase."
      Yan B., Yang D., Brady M., Parkinson A.
      J. Biol. Chem. 269:29688-29696(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiEST4_RAT
    AccessioniPrimary (citable) accession number: Q64573
    Secondary accession number(s): A2VCW1, Q62679
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3