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Q64568 (AT2B3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasma membrane calcium-transporting ATPase 3

Short name=PMCA3
EC=3.6.3.8
Alternative name(s):
Plasma membrane calcium ATPase isoform 3
Plasma membrane calcium pump isoform 3
Gene names
Name:Atp2b3
Synonyms:Pmca3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1258 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activity

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Subunit structure

Interacts with PDZD11 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Isoform XA is the most abundant form in brain and most other tissues. Isoform XB is the most abundant form in skeletal muscle and is also found in brain and at low levels in testis and kidney. The other isoforms are only found at low levels in various tissues.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Alternative products

This entry describes 12 isoforms produced by alternative splicing. [Align] [Select]

Note: There is a combination of two alternative spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A, B, C, D, E and F). So far the splice sites have been studied independently. Experimental confirmation may be lacking for some isoforms.
Isoform XD (identifier: Q64568-1)

Also known as: AIICIV;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform XA (identifier: Q64568-2)

Also known as: AIICII;

The sequence of this isoform differs from the canonical sequence as follows:
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP
Isoform ZA (identifier: Q64568-3)

Also known as: AICII;

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP
Isoform XB (identifier: Q64568-4)

Also known as: AIICI;

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1152: Missing.
Isoform ZB (identifier: Q64568-5)

Also known as: AICI;

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1115-1152: Missing.
Isoform XC (identifier: Q64568-6)

Also known as: AIICIII;

The sequence of this isoform differs from the canonical sequence as follows:
     1144-1152: Missing.
Isoform ZC (identifier: Q64568-7)

Also known as: AICIII;

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1144-1152: Missing.
Isoform ZD (identifier: Q64568-8)

Also known as: AICIV;

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
Isoform XE (identifier: Q64568-9)

Also known as: AIICV;

The sequence of this isoform differs from the canonical sequence as follows:
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES
Isoform ZE (identifier: Q64568-10)

Also known as: AICV;

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES
Isoform XF (identifier: Q64568-11)

Also known as: AIICVI;

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG
Isoform ZF (identifier: Q64568-12)

Also known as: AICVI;

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12581258Plasma membrane calcium-transporting ATPase 3
PRO_0000046219

Regions

Topological domain1 – 9797Cytoplasmic Potential
Transmembrane98 – 11821Helical; Potential
Topological domain119 – 15537Extracellular Potential
Transmembrane156 – 17621Helical; Potential
Topological domain177 – 364188Cytoplasmic Potential
Transmembrane365 – 38420Helical; Potential
Topological domain385 – 41733Extracellular Potential
Transmembrane418 – 43518Helical; Potential
Topological domain436 – 849414Cytoplasmic Potential
Transmembrane850 – 86920Helical; Potential
Topological domain870 – 87910Extracellular Potential
Transmembrane880 – 90021Helical; Potential
Topological domain901 – 92020Cytoplasmic Potential
Transmembrane921 – 94323Helical; Potential
Topological domain944 – 96118Extracellular Potential
Transmembrane962 – 98322Helical; Potential
Topological domain984 – 100219Cytoplasmic Potential
Transmembrane1003 – 102422Helical; Potential
Topological domain1025 – 103410Extracellular Potential
Transmembrane1035 – 105622Helical; Potential
Topological domain1057 – 1258202Cytoplasmic Potential
Region1097 – 111418Calmodulin-binding subdomain A By similarity
Region1115 – 112410Calmodulin-binding subdomain B By similarity
Compositional bias297 – 3004Poly-Glu
Compositional bias1212 – 12176Poly-Pro

Sites

Active site47314-aspartylphosphate intermediate By similarity
Metal binding7941Magnesium By similarity
Metal binding7981Magnesium By similarity

Amino acid modifications

Modified residue11131Phosphothreonine; by PKC By similarity

Natural variations

Alternative sequence306 – 31914Missing in isoform ZA, isoform ZB, isoform ZC, isoform ZD, isoform ZE and isoform ZF.
VSP_000396
Alternative sequence1115 – 1258144MEVVS…METSL → VCWDGKKMLRTTEVG in isoform XF and isoform ZF.
VSP_000398
Alternative sequence1115 – 115238Missing in isoform XB and isoform ZB.
VSP_000397
Alternative sequence1144 – 11529Missing in isoform XC and isoform ZC.
VSP_000399
Alternative sequence1153 – 1258106IRVVK…METSL → VTLSAAKPTSAAGNPSGESI P in isoform XA and isoform ZA.
VSP_000400
Alternative sequence1153 – 1258106IRVVK…METSL → VTLSAAKPTSAAGSES in isoform XE and isoform ZE.
VSP_000401

Sequences

Sequence LengthMass (Da)Tools
Isoform XD (AIICIV) [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 3A13781D21042BA6

FASTA1,258138,559
        10         20         30         40         50         60 
MGDMANSSIE FHPKPQQQRE VPHVGGFGCT LAELRSLMEL RGAEALQKIQ EAYGDVSGLC 

        70         80         90        100        110        120 
RRLKTSPTEG LADNTNDLEK RRQIYGQNFI PPKQPKTFLQ LVWEALQDVT LIILEVAAIV 

       130        140        150        160        170        180 
SLGLSFYAPP GEESEACGNV SGGAEDEGEA EAGWIEGAAI LLSVICVVLV TAFNDWSKEK 

       190        200        210        220        230        240 
QFRGLQSRIE QEQKFTVIRN GQLLQVPVAA LVVGDIAQVK YGDLLPADGV LIQGNDLKID 

       250        260        270        280        290        300 
ESSLTGESDH VRKSADKDPM LLSGTHVMEG SGRMVVTAVG VNSQTGIIFT LLGAGGEEEE 

       310        320        330        340        350        360 
KKDKKGKQQD GAMESSQTKA KKQDGAVAME MQPLKSAEGG EMEEREKKKA NVPKKEKSVL 

       370        380        390        400        410        420 
QGKLTKLAVQ IGKAGLVMSA ITVIILVLYF VIETFVVDGR VWLAECTPVY VQYFVKFFII 

       430        440        450        460        470        480 
GVTVLVVAVP EGLPLAVTIS LAYSVKKMMK DNNLVRHLDA CETMGNATAI CSDKTGTLTT 

       490        500        510        520        530        540 
NRMTVVQSYL GDTHYKEIPA PSALTPKILD LLVHAISINS AYTTKILPPE KEGALPRQVG 

       550        560        570        580        590        600 
NKTECALLGF ILDLKRDFQP VREQIPEDQL YKVYTFNSVR KSMSTVIRMP DGGFRLFSKG 

       610        620        630        640        650        660 
ASEILLKKCT NILNSNGELR GFRPRDRDDM VKKIIEPMAC DGLRTICIAY RDFSAIQEPD 

       670        680        690        700        710        720 
WDNENEVVGD LTCIAVVGIE DPVRPEVPEA IRKCQRAGIT VRMVTGDNIN TARAIAAKCG 

       730        740        750        760        770        780 
IIQPGEDFLC LEGKEFNRRI RNEKGEIEQE RLDKVWPKLR VLARSSPTDK HTLVKGIIDS 

       790        800        810        820        830        840 
TTGEQRQVVA VTGDGTNDGP ALKKADVGFA MGIAGTDVAK EASDIILTDD NFTSIVKAVM 

       850        860        870        880        890        900 
WGRNVYDSIS KFLQFQLTVN VVAVIVAFTG ACITQDSPLK AVQMLWVNLI MDTFASLALA 

       910        920        930        940        950        960 
TEPPTESLLL RKPYGRDKPL ISRTMMKNIL GHAVYQLTII FTLLFVGELF FDIDSGRNAP 

       970        980        990       1000       1010       1020 
LHSPPSEHYT IIFNTFVMMQ LFNEINARKI HGERNVFDGI FSNPIFCTIV LGTFGIQIVI 

      1030       1040       1050       1060       1070       1080 
VQFGGKPFSC SPLSTEQWLW CLFVGVGELV WGQVIATIPT SQLKCLKEAG HGPGKDEMTD 

      1090       1100       1110       1120       1130       1140 
EELAEGEEEI DHAERELRRG QILWFRGLNR IQTQMEVVST FKRSGSFQGA VRRRSSVLSQ 

      1150       1160       1170       1180       1190       1200 
LHDVTNLSTP THIRVVKAFR SSLYEGLEKP ESKSCIHNFM ATPEFLINDY THNIPLIDDT 

      1210       1220       1230       1240       1250 
DVDENEERLR APPPPPPNQN NNAIDSGIYL TTHATKSATS SAFSSRPGSP LHSMETSL 

« Hide

Isoform XA (AIICII) [UniParc].

Checksum: CF49538EC579E958
Show »

FASTA1,173128,773
Isoform ZA (AICII) [UniParc].

Checksum: A33A52545101241D
Show »

FASTA1,159127,297
Isoform XB (AIICI) [UniParc].

Checksum: C18A4BAEBA0362ED
Show »

FASTA1,220134,360
Isoform ZB (AICI) [UniParc].

Checksum: F83F2E1301CB4552
Show »

FASTA1,206132,884
Isoform XC (AIICIII) [UniParc].

Checksum: 9825517A8B9744B1
Show »

FASTA1,249137,608
Isoform ZC (AICIII) [UniParc].

Checksum: DD7B43D805121FDD
Show »

FASTA1,235136,132
Isoform ZD (AICIV) [UniParc].

Checksum: 3BA47725ACB4D4AE
Show »

FASTA1,244137,083
Isoform XE (AIICV) [UniParc].

Checksum: 09F539391396F9A2
Show »

FASTA1,168128,295
Isoform ZE (AICV) [UniParc].

Checksum: 81387C79448BCC0F
Show »

FASTA1,154126,818
Isoform XF (AIICVI) [UniParc].

Checksum: 7B4F423A0C709026
Show »

FASTA1,129124,342
Isoform ZF (AICVI) [UniParc].

Checksum: 1A545F41D1A951EB
Show »

FASTA1,115122,866

References

[1]"Molecular cloning of a third isoform of the calmodulin-sensitive plasma membrane Ca2+-transporting ATPase that is expressed predominantly in brain and skeletal muscle."
Greeb J., Shull G.E.
J. Biol. Chem. 264:18569-18576(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ZA).
Tissue: Brain.
[2]"Structure of the rat plasma membrane Ca(2+)-ATPase isoform 3 gene and characterization of alternative splicing and transcription products. Skeletal muscle-specific splicing results in a plasma membrane Ca(2+)-ATPase with a novel calmodulin-binding domain."
Burk S.E., Shull G.E.
J. Biol. Chem. 267:19683-19690(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1016-1258 (ISOFORMS XF/ZF), ALTERNATIVE SPLICING.
Strain: CD Charles River.
[3]"Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
Keeton T.P., Burk S.E., Shull G.E.
J. Biol. Chem. 268:2740-2748(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1054-1258, ALTERNATIVE SPLICING (ISOFORMS XA/ZA).
Strain: CD Charles River.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L05565, L05558, L05564 Genomic DNA. Translation: AAA53650.1.
J05087 mRNA. Translation: AAA69667.1.
M96626 mRNA. Translation: AAA50821.1.
PIRA34308.
C44525.
RefSeqNP_579822.1. NM_133288.1.
UniGeneRn.11053.

3D structure databases

ProteinModelPortalQ64568.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248231. 1 interaction.

PTM databases

PhosphoSiteQ64568.

Proteomic databases

PaxDbQ64568.
PRIDEQ64568.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023934; ENSRNOP00000023934; ENSRNOG00000017798. [Q64568-3]
GeneID29599.
KEGGrno:29599.

Organism-specific databases

CTD492.
RGD621304. Atp2b3.

Phylogenomic databases

eggNOGCOG0474.
GeneTreeENSGT00510000046331.
HOGENOMHOG000265623.
HOVERGENHBG061286.
InParanoidQ64568.
KOK05850.

Gene expression databases

ArrayExpressQ64568.
GenevestigatorQ64568.

Family and domain databases

Gene3D1.20.1110.10. 3 hits.
InterProIPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio609754.
PROQ64568.

Entry information

Entry nameAT2B3_RAT
AccessionPrimary (citable) accession number: Q64568
Secondary accession number(s): Q01489, Q63444
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: March 19, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families