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Q64568

- AT2B3_RAT

UniProt

Q64568 - AT2B3_RAT

Protein

Plasma membrane calcium-transporting ATPase 3

Gene

Atp2b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

    Catalytic activityi

    ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei473 – 47314-aspartylphosphate intermediateBy similarity
    Metal bindingi794 – 7941MagnesiumBy similarity
    Metal bindingi798 – 7981MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium-transporting ATPase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. PDZ domain binding Source: RGD
    5. protein binding Source: UniProtKB

    GO - Biological processi

    1. brain development Source: RGD
    2. neural retina development Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plasma membrane calcium-transporting ATPase 3 (EC:3.6.3.8)
    Short name:
    PMCA3
    Alternative name(s):
    Plasma membrane calcium ATPase isoform 3
    Plasma membrane calcium pump isoform 3
    Gene namesi
    Name:Atp2b3
    Synonyms:Pmca3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi621304. Atp2b3.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. membrane raft Source: RGD
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12581258Plasma membrane calcium-transporting ATPase 3PRO_0000046219Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1113 – 11131Phosphothreonine; by PKCBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ64568.
    PRIDEiQ64568.

    PTM databases

    PhosphoSiteiQ64568.

    Expressioni

    Tissue specificityi

    Isoform XA is the most abundant form in brain and most other tissues. Isoform XB is the most abundant form in skeletal muscle and is also found in brain and at low levels in testis and kidney. The other isoforms are only found at low levels in various tissues.

    Gene expression databases

    ArrayExpressiQ64568.
    GenevestigatoriQ64568.

    Interactioni

    Subunit structurei

    Interacts with PDZD11.By similarity

    Protein-protein interaction databases

    BioGridi248231. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64568.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9797CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini119 – 15537ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini177 – 364188CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini385 – 41733ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini436 – 849414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini870 – 87910ExtracellularSequence Analysis
    Topological domaini901 – 92020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini944 – 96118ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini984 – 100219CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1025 – 103410ExtracellularSequence Analysis
    Topological domaini1057 – 1258202CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei98 – 11821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei156 – 17621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei365 – 38420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei418 – 43518HelicalSequence AnalysisAdd
    BLAST
    Transmembranei850 – 86920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei880 – 90021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei921 – 94323HelicalSequence AnalysisAdd
    BLAST
    Transmembranei962 – 98322HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1003 – 102422HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1035 – 105622HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1097 – 111418Calmodulin-binding subdomain ABy similarityAdd
    BLAST
    Regioni1115 – 112410Calmodulin-binding subdomain BBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi297 – 3004Poly-Glu
    Compositional biasi1212 – 12176Poly-Pro

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00510000046331.
    HOGENOMiHOG000265623.
    HOVERGENiHBG061286.
    InParanoidiQ64568.
    KOiK05850.
    PhylomeDBiQ64568.

    Family and domain databases

    Gene3Di1.20.1110.10. 3 hits.
    InterProiIPR022141. ATP_Ca_trans_C.
    IPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF12424. ATP_Ca_trans_C. 2 hits.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequences (12)i

    Sequence statusi: Complete.

    This entry describes 12 isoformsi produced by alternative splicing. Align

    Note: There is a combination of two alternative spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A, B, C, D, E and F). So far the splice sites have been studied independently. Experimental confirmation may be lacking for some isoforms.

    Isoform XD (identifier: Q64568-1) [UniParc]FASTAAdd to Basket

    Also known as: AIICIV

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGDMANSSIE FHPKPQQQRE VPHVGGFGCT LAELRSLMEL RGAEALQKIQ     50
    EAYGDVSGLC RRLKTSPTEG LADNTNDLEK RRQIYGQNFI PPKQPKTFLQ 100
    LVWEALQDVT LIILEVAAIV SLGLSFYAPP GEESEACGNV SGGAEDEGEA 150
    EAGWIEGAAI LLSVICVVLV TAFNDWSKEK QFRGLQSRIE QEQKFTVIRN 200
    GQLLQVPVAA LVVGDIAQVK YGDLLPADGV LIQGNDLKID ESSLTGESDH 250
    VRKSADKDPM LLSGTHVMEG SGRMVVTAVG VNSQTGIIFT LLGAGGEEEE 300
    KKDKKGKQQD GAMESSQTKA KKQDGAVAME MQPLKSAEGG EMEEREKKKA 350
    NVPKKEKSVL QGKLTKLAVQ IGKAGLVMSA ITVIILVLYF VIETFVVDGR 400
    VWLAECTPVY VQYFVKFFII GVTVLVVAVP EGLPLAVTIS LAYSVKKMMK 450
    DNNLVRHLDA CETMGNATAI CSDKTGTLTT NRMTVVQSYL GDTHYKEIPA 500
    PSALTPKILD LLVHAISINS AYTTKILPPE KEGALPRQVG NKTECALLGF 550
    ILDLKRDFQP VREQIPEDQL YKVYTFNSVR KSMSTVIRMP DGGFRLFSKG 600
    ASEILLKKCT NILNSNGELR GFRPRDRDDM VKKIIEPMAC DGLRTICIAY 650
    RDFSAIQEPD WDNENEVVGD LTCIAVVGIE DPVRPEVPEA IRKCQRAGIT 700
    VRMVTGDNIN TARAIAAKCG IIQPGEDFLC LEGKEFNRRI RNEKGEIEQE 750
    RLDKVWPKLR VLARSSPTDK HTLVKGIIDS TTGEQRQVVA VTGDGTNDGP 800
    ALKKADVGFA MGIAGTDVAK EASDIILTDD NFTSIVKAVM WGRNVYDSIS 850
    KFLQFQLTVN VVAVIVAFTG ACITQDSPLK AVQMLWVNLI MDTFASLALA 900
    TEPPTESLLL RKPYGRDKPL ISRTMMKNIL GHAVYQLTII FTLLFVGELF 950
    FDIDSGRNAP LHSPPSEHYT IIFNTFVMMQ LFNEINARKI HGERNVFDGI 1000
    FSNPIFCTIV LGTFGIQIVI VQFGGKPFSC SPLSTEQWLW CLFVGVGELV 1050
    WGQVIATIPT SQLKCLKEAG HGPGKDEMTD EELAEGEEEI DHAERELRRG 1100
    QILWFRGLNR IQTQMEVVST FKRSGSFQGA VRRRSSVLSQ LHDVTNLSTP 1150
    THIRVVKAFR SSLYEGLEKP ESKSCIHNFM ATPEFLINDY THNIPLIDDT 1200
    DVDENEERLR APPPPPPNQN NNAIDSGIYL TTHATKSATS SAFSSRPGSP 1250
    LHSMETSL 1258
    Length:1,258
    Mass (Da):138,559
    Last modified:June 1, 2001 - v2
    Checksum:i3A13781D21042BA6
    GO
    Isoform XA (identifier: Q64568-2) [UniParc]FASTAAdd to Basket

    Also known as: AIICII

    The sequence of this isoform differs from the canonical sequence as follows:
         1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP

    Show »
    Length:1,173
    Mass (Da):128,773
    Checksum:iCF49538EC579E958
    GO
    Isoform ZA (identifier: Q64568-3) [UniParc]FASTAAdd to Basket

    Also known as: AICII

    The sequence of this isoform differs from the canonical sequence as follows:
         306-319: Missing.
         1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP

    Show »
    Length:1,159
    Mass (Da):127,297
    Checksum:iA33A52545101241D
    GO
    Isoform XB (identifier: Q64568-4) [UniParc]FASTAAdd to Basket

    Also known as: AIICI

    The sequence of this isoform differs from the canonical sequence as follows:
         1115-1152: Missing.

    Show »
    Length:1,220
    Mass (Da):134,360
    Checksum:iC18A4BAEBA0362ED
    GO
    Isoform ZB (identifier: Q64568-5) [UniParc]FASTAAdd to Basket

    Also known as: AICI

    The sequence of this isoform differs from the canonical sequence as follows:
         306-319: Missing.
         1115-1152: Missing.

    Show »
    Length:1,206
    Mass (Da):132,884
    Checksum:iF83F2E1301CB4552
    GO
    Isoform XC (identifier: Q64568-6) [UniParc]FASTAAdd to Basket

    Also known as: AIICIII

    The sequence of this isoform differs from the canonical sequence as follows:
         1144-1152: Missing.

    Show »
    Length:1,249
    Mass (Da):137,608
    Checksum:i9825517A8B9744B1
    GO
    Isoform ZC (identifier: Q64568-7) [UniParc]FASTAAdd to Basket

    Also known as: AICIII

    The sequence of this isoform differs from the canonical sequence as follows:
         306-319: Missing.
         1144-1152: Missing.

    Show »
    Length:1,235
    Mass (Da):136,132
    Checksum:iDD7B43D805121FDD
    GO
    Isoform ZD (identifier: Q64568-8) [UniParc]FASTAAdd to Basket

    Also known as: AICIV

    The sequence of this isoform differs from the canonical sequence as follows:
         306-319: Missing.

    Show »
    Length:1,244
    Mass (Da):137,083
    Checksum:i3BA47725ACB4D4AE
    GO
    Isoform XE (identifier: Q64568-9) [UniParc]FASTAAdd to Basket

    Also known as: AIICV

    The sequence of this isoform differs from the canonical sequence as follows:
         1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES

    Show »
    Length:1,168
    Mass (Da):128,295
    Checksum:i09F539391396F9A2
    GO
    Isoform ZE (identifier: Q64568-10) [UniParc]FASTAAdd to Basket

    Also known as: AICV

    The sequence of this isoform differs from the canonical sequence as follows:
         306-319: Missing.
         1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES

    Show »
    Length:1,154
    Mass (Da):126,818
    Checksum:i81387C79448BCC0F
    GO
    Isoform XF (identifier: Q64568-11) [UniParc]FASTAAdd to Basket

    Also known as: AIICVI

    The sequence of this isoform differs from the canonical sequence as follows:
         1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

    Show »
    Length:1,129
    Mass (Da):124,342
    Checksum:i7B4F423A0C709026
    GO
    Isoform ZF (identifier: Q64568-12) [UniParc]FASTAAdd to Basket

    Also known as: AICVI

    The sequence of this isoform differs from the canonical sequence as follows:
         306-319: Missing.
         1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

    Show »
    Length:1,115
    Mass (Da):122,866
    Checksum:i1A545F41D1A951EB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei306 – 31914Missing in isoform ZA, isoform ZB, isoform ZC, isoform ZD, isoform ZE and isoform ZF. 1 PublicationVSP_000396Add
    BLAST
    Alternative sequencei1115 – 1258144MEVVS…METSL → VCWDGKKMLRTTEVG in isoform XF and isoform ZF. CuratedVSP_000398Add
    BLAST
    Alternative sequencei1115 – 115238Missing in isoform XB and isoform ZB. CuratedVSP_000397Add
    BLAST
    Alternative sequencei1144 – 11529Missing in isoform XC and isoform ZC. CuratedVSP_000399
    Alternative sequencei1153 – 1258106IRVVK…METSL → VTLSAAKPTSAAGNPSGESI P in isoform XA and isoform ZA. 1 PublicationVSP_000400Add
    BLAST
    Alternative sequencei1153 – 1258106IRVVK…METSL → VTLSAAKPTSAAGSES in isoform XE and isoform ZE. CuratedVSP_000401Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05565, L05558, L05564 Genomic DNA. Translation: AAA53650.1.
    J05087 mRNA. Translation: AAA69667.1.
    M96626 mRNA. Translation: AAA50821.1.
    PIRiA34308.
    C44525.
    RefSeqiNP_579822.1. NM_133288.1. [Q64568-3]
    UniGeneiRn.11053.

    Genome annotation databases

    EnsembliENSRNOT00000023934; ENSRNOP00000023934; ENSRNOG00000017798. [Q64568-3]
    GeneIDi29599.
    KEGGirno:29599.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05565 , L05558 , L05564 Genomic DNA. Translation: AAA53650.1 .
    J05087 mRNA. Translation: AAA69667.1 .
    M96626 mRNA. Translation: AAA50821.1 .
    PIRi A34308.
    C44525.
    RefSeqi NP_579822.1. NM_133288.1. [Q64568-3 ]
    UniGenei Rn.11053.

    3D structure databases

    ProteinModelPortali Q64568.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248231. 1 interaction.

    PTM databases

    PhosphoSitei Q64568.

    Proteomic databases

    PaxDbi Q64568.
    PRIDEi Q64568.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023934 ; ENSRNOP00000023934 ; ENSRNOG00000017798 . [Q64568-3 ]
    GeneIDi 29599.
    KEGGi rno:29599.

    Organism-specific databases

    CTDi 492.
    RGDi 621304. Atp2b3.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00510000046331.
    HOGENOMi HOG000265623.
    HOVERGENi HBG061286.
    InParanoidi Q64568.
    KOi K05850.
    PhylomeDBi Q64568.

    Miscellaneous databases

    NextBioi 609754.
    PROi Q64568.

    Gene expression databases

    ArrayExpressi Q64568.
    Genevestigatori Q64568.

    Family and domain databases

    Gene3Di 1.20.1110.10. 3 hits.
    InterProi IPR022141. ATP_Ca_trans_C.
    IPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF12424. ATP_Ca_trans_C. 2 hits.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a third isoform of the calmodulin-sensitive plasma membrane Ca2+-transporting ATPase that is expressed predominantly in brain and skeletal muscle."
      Greeb J., Shull G.E.
      J. Biol. Chem. 264:18569-18576(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ZA).
      Tissue: Brain.
    2. "Structure of the rat plasma membrane Ca(2+)-ATPase isoform 3 gene and characterization of alternative splicing and transcription products. Skeletal muscle-specific splicing results in a plasma membrane Ca(2+)-ATPase with a novel calmodulin-binding domain."
      Burk S.E., Shull G.E.
      J. Biol. Chem. 267:19683-19690(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1016-1258 (ISOFORMS XF/ZF), ALTERNATIVE SPLICING.
      Strain: CD Charles River.
    3. "Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
      Keeton T.P., Burk S.E., Shull G.E.
      J. Biol. Chem. 268:2740-2748(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1054-1258, ALTERNATIVE SPLICING (ISOFORMS XA/ZA).
      Strain: CD Charles River.

    Entry informationi

    Entry nameiAT2B3_RAT
    AccessioniPrimary (citable) accession number: Q64568
    Secondary accession number(s): Q01489, Q63444
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3