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Protein

Plasma membrane calcium-transporting ATPase 3

Gene

Atp2b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4734-aspartylphosphate intermediateBy similarity1
Metal bindingi794MagnesiumBy similarity1
Metal bindingi798MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • PDZ domain binding Source: RGD

GO - Biological processi

  • brain development Source: RGD
  • cellular calcium ion homeostasis Source: GO_Central
  • neural retina development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-418359. Reduction of cytosolic Ca++ levels.
R-RNO-5578775. Ion homeostasis.
R-RNO-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 3 (EC:3.6.3.8)
Short name:
PMCA3
Alternative name(s):
Plasma membrane calcium ATPase isoform 3
Plasma membrane calcium pump isoform 3
Gene namesi
Name:Atp2b3
Synonyms:Pmca3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi621304. Atp2b3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 97CytoplasmicSequence analysisAdd BLAST97
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 155ExtracellularSequence analysisAdd BLAST37
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Topological domaini177 – 364CytoplasmicSequence analysisAdd BLAST188
Transmembranei365 – 384HelicalSequence analysisAdd BLAST20
Topological domaini385 – 417ExtracellularSequence analysisAdd BLAST33
Transmembranei418 – 435HelicalSequence analysisAdd BLAST18
Topological domaini436 – 849CytoplasmicSequence analysisAdd BLAST414
Transmembranei850 – 869HelicalSequence analysisAdd BLAST20
Topological domaini870 – 879ExtracellularSequence analysis10
Transmembranei880 – 900HelicalSequence analysisAdd BLAST21
Topological domaini901 – 920CytoplasmicSequence analysisAdd BLAST20
Transmembranei921 – 943HelicalSequence analysisAdd BLAST23
Topological domaini944 – 961ExtracellularSequence analysisAdd BLAST18
Transmembranei962 – 983HelicalSequence analysisAdd BLAST22
Topological domaini984 – 1002CytoplasmicSequence analysisAdd BLAST19
Transmembranei1003 – 1024HelicalSequence analysisAdd BLAST22
Topological domaini1025 – 1034ExtracellularSequence analysis10
Transmembranei1035 – 1056HelicalSequence analysisAdd BLAST22
Topological domaini1057 – 1258CytoplasmicSequence analysisAdd BLAST202

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462191 – 1258Plasma membrane calcium-transporting ATPase 3Add BLAST1258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1
Modified residuei1079PhosphothreonineCombined sources1
Modified residuei1113Phosphothreonine; by PKCBy similarity1
Modified residuei1126PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64568.
PRIDEiQ64568.

PTM databases

iPTMnetiQ64568.
PhosphoSitePlusiQ64568.
SwissPalmiQ64568.

Expressioni

Tissue specificityi

Isoform XA is the most abundant form in brain and most other tissues. Isoform XB is the most abundant form in skeletal muscle and is also found in brain and at low levels in testis and kidney. The other isoforms are only found at low levels in various tissues.

Gene expression databases

BgeeiENSRNOG00000017798.
ExpressionAtlasiQ64568. baseline.

Interactioni

Subunit structurei

Interacts with PDZD11.By similarity

GO - Molecular functioni

  • PDZ domain binding Source: RGD

Protein-protein interaction databases

BioGridi248231. 1 interactor.
STRINGi10116.ENSRNOP00000035695.

Structurei

3D structure databases

ProteinModelPortaliQ64568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1097 – 1114Calmodulin-binding subdomain ABy similarityAdd BLAST18
Regioni1115 – 1124Calmodulin-binding subdomain BBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi297 – 300Poly-Glu4
Compositional biasi1212 – 1217Poly-Pro6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ64568.
KOiK05850.
PhylomeDBiQ64568.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (12)i

Sequence statusi: Complete.

This entry describes 12 isoformsi produced by alternative splicing. AlignAdd to basket

Note: There is a combination of two alternative spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A, B, C, D, E and F). So far the splice sites have been studied independently. Experimental confirmation may be lacking for some isoforms.
Isoform XD (identifier: Q64568-1) [UniParc]FASTAAdd to basket
Also known as: AIICIV

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMANSSIE FHPKPQQQRE VPHVGGFGCT LAELRSLMEL RGAEALQKIQ
60 70 80 90 100
EAYGDVSGLC RRLKTSPTEG LADNTNDLEK RRQIYGQNFI PPKQPKTFLQ
110 120 130 140 150
LVWEALQDVT LIILEVAAIV SLGLSFYAPP GEESEACGNV SGGAEDEGEA
160 170 180 190 200
EAGWIEGAAI LLSVICVVLV TAFNDWSKEK QFRGLQSRIE QEQKFTVIRN
210 220 230 240 250
GQLLQVPVAA LVVGDIAQVK YGDLLPADGV LIQGNDLKID ESSLTGESDH
260 270 280 290 300
VRKSADKDPM LLSGTHVMEG SGRMVVTAVG VNSQTGIIFT LLGAGGEEEE
310 320 330 340 350
KKDKKGKQQD GAMESSQTKA KKQDGAVAME MQPLKSAEGG EMEEREKKKA
360 370 380 390 400
NVPKKEKSVL QGKLTKLAVQ IGKAGLVMSA ITVIILVLYF VIETFVVDGR
410 420 430 440 450
VWLAECTPVY VQYFVKFFII GVTVLVVAVP EGLPLAVTIS LAYSVKKMMK
460 470 480 490 500
DNNLVRHLDA CETMGNATAI CSDKTGTLTT NRMTVVQSYL GDTHYKEIPA
510 520 530 540 550
PSALTPKILD LLVHAISINS AYTTKILPPE KEGALPRQVG NKTECALLGF
560 570 580 590 600
ILDLKRDFQP VREQIPEDQL YKVYTFNSVR KSMSTVIRMP DGGFRLFSKG
610 620 630 640 650
ASEILLKKCT NILNSNGELR GFRPRDRDDM VKKIIEPMAC DGLRTICIAY
660 670 680 690 700
RDFSAIQEPD WDNENEVVGD LTCIAVVGIE DPVRPEVPEA IRKCQRAGIT
710 720 730 740 750
VRMVTGDNIN TARAIAAKCG IIQPGEDFLC LEGKEFNRRI RNEKGEIEQE
760 770 780 790 800
RLDKVWPKLR VLARSSPTDK HTLVKGIIDS TTGEQRQVVA VTGDGTNDGP
810 820 830 840 850
ALKKADVGFA MGIAGTDVAK EASDIILTDD NFTSIVKAVM WGRNVYDSIS
860 870 880 890 900
KFLQFQLTVN VVAVIVAFTG ACITQDSPLK AVQMLWVNLI MDTFASLALA
910 920 930 940 950
TEPPTESLLL RKPYGRDKPL ISRTMMKNIL GHAVYQLTII FTLLFVGELF
960 970 980 990 1000
FDIDSGRNAP LHSPPSEHYT IIFNTFVMMQ LFNEINARKI HGERNVFDGI
1010 1020 1030 1040 1050
FSNPIFCTIV LGTFGIQIVI VQFGGKPFSC SPLSTEQWLW CLFVGVGELV
1060 1070 1080 1090 1100
WGQVIATIPT SQLKCLKEAG HGPGKDEMTD EELAEGEEEI DHAERELRRG
1110 1120 1130 1140 1150
QILWFRGLNR IQTQMEVVST FKRSGSFQGA VRRRSSVLSQ LHDVTNLSTP
1160 1170 1180 1190 1200
THIRVVKAFR SSLYEGLEKP ESKSCIHNFM ATPEFLINDY THNIPLIDDT
1210 1220 1230 1240 1250
DVDENEERLR APPPPPPNQN NNAIDSGIYL TTHATKSATS SAFSSRPGSP

LHSMETSL
Length:1,258
Mass (Da):138,559
Last modified:June 1, 2001 - v2
Checksum:i3A13781D21042BA6
GO
Isoform XA (identifier: Q64568-2) [UniParc]FASTAAdd to basket
Also known as: AIICII

The sequence of this isoform differs from the canonical sequence as follows:
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP

Show »
Length:1,173
Mass (Da):128,773
Checksum:iCF49538EC579E958
GO
Isoform ZA (identifier: Q64568-3) [UniParc]FASTAAdd to basket
Also known as: AICII

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP

Show »
Length:1,159
Mass (Da):127,297
Checksum:iA33A52545101241D
GO
Isoform XB (identifier: Q64568-4) [UniParc]FASTAAdd to basket
Also known as: AIICI

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1152: Missing.

Show »
Length:1,220
Mass (Da):134,360
Checksum:iC18A4BAEBA0362ED
GO
Isoform ZB (identifier: Q64568-5) [UniParc]FASTAAdd to basket
Also known as: AICI

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1115-1152: Missing.

Show »
Length:1,206
Mass (Da):132,884
Checksum:iF83F2E1301CB4552
GO
Isoform XC (identifier: Q64568-6) [UniParc]FASTAAdd to basket
Also known as: AIICIII

The sequence of this isoform differs from the canonical sequence as follows:
     1144-1152: Missing.

Show »
Length:1,249
Mass (Da):137,608
Checksum:i9825517A8B9744B1
GO
Isoform ZC (identifier: Q64568-7) [UniParc]FASTAAdd to basket
Also known as: AICIII

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1144-1152: Missing.

Show »
Length:1,235
Mass (Da):136,132
Checksum:iDD7B43D805121FDD
GO
Isoform ZD (identifier: Q64568-8) [UniParc]FASTAAdd to basket
Also known as: AICIV

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.

Show »
Length:1,244
Mass (Da):137,083
Checksum:i3BA47725ACB4D4AE
GO
Isoform XE (identifier: Q64568-9) [UniParc]FASTAAdd to basket
Also known as: AIICV

The sequence of this isoform differs from the canonical sequence as follows:
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES

Show »
Length:1,168
Mass (Da):128,295
Checksum:i09F539391396F9A2
GO
Isoform ZE (identifier: Q64568-10) [UniParc]FASTAAdd to basket
Also known as: AICV

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES

Show »
Length:1,154
Mass (Da):126,818
Checksum:i81387C79448BCC0F
GO
Isoform XF (identifier: Q64568-11) [UniParc]FASTAAdd to basket
Also known as: AIICVI

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

Show »
Length:1,129
Mass (Da):124,342
Checksum:i7B4F423A0C709026
GO
Isoform ZF (identifier: Q64568-12) [UniParc]FASTAAdd to basket
Also known as: AICVI

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

Show »
Length:1,115
Mass (Da):122,866
Checksum:i1A545F41D1A951EB
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000396306 – 319Missing in isoform ZA, isoform ZB, isoform ZC, isoform ZD, isoform ZE and isoform ZF. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_0003981115 – 1258MEVVS…METSL → VCWDGKKMLRTTEVG in isoform XF and isoform ZF. CuratedAdd BLAST144
Alternative sequenceiVSP_0003971115 – 1152Missing in isoform XB and isoform ZB. CuratedAdd BLAST38
Alternative sequenceiVSP_0003991144 – 1152Missing in isoform XC and isoform ZC. Curated9
Alternative sequenceiVSP_0004001153 – 1258IRVVK…METSL → VTLSAAKPTSAAGNPSGESI P in isoform XA and isoform ZA. 1 PublicationAdd BLAST106
Alternative sequenceiVSP_0004011153 – 1258IRVVK…METSL → VTLSAAKPTSAAGSES in isoform XE and isoform ZE. CuratedAdd BLAST106

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05565, L05558, L05564 Genomic DNA. Translation: AAA53650.1.
J05087 mRNA. Translation: AAA69667.1.
M96626 mRNA. Translation: AAA50821.1.
PIRiA34308.
C44525.
RefSeqiNP_579822.1. NM_133288.1. [Q64568-3]
XP_008771842.1. XM_008773620.2. [Q64568-7]
XP_017457435.1. XM_017601946.1. [Q64568-5]
XP_017457436.1. XM_017601947.1. [Q64568-3]
XP_017457437.1. XM_017601948.1. [Q64568-3]
UniGeneiRn.11053.

Genome annotation databases

EnsembliENSRNOT00000077979; ENSRNOP00000071471; ENSRNOG00000061304. [Q64568-3]
GeneIDi29599.
KEGGirno:29599.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05565, L05558, L05564 Genomic DNA. Translation: AAA53650.1.
J05087 mRNA. Translation: AAA69667.1.
M96626 mRNA. Translation: AAA50821.1.
PIRiA34308.
C44525.
RefSeqiNP_579822.1. NM_133288.1. [Q64568-3]
XP_008771842.1. XM_008773620.2. [Q64568-7]
XP_017457435.1. XM_017601946.1. [Q64568-5]
XP_017457436.1. XM_017601947.1. [Q64568-3]
XP_017457437.1. XM_017601948.1. [Q64568-3]
UniGeneiRn.11053.

3D structure databases

ProteinModelPortaliQ64568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248231. 1 interactor.
STRINGi10116.ENSRNOP00000035695.

PTM databases

iPTMnetiQ64568.
PhosphoSitePlusiQ64568.
SwissPalmiQ64568.

Proteomic databases

PaxDbiQ64568.
PRIDEiQ64568.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000077979; ENSRNOP00000071471; ENSRNOG00000061304. [Q64568-3]
GeneIDi29599.
KEGGirno:29599.

Organism-specific databases

CTDi492.
RGDi621304. Atp2b3.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ64568.
KOiK05850.
PhylomeDBiQ64568.

Enzyme and pathway databases

ReactomeiR-RNO-418359. Reduction of cytosolic Ca++ levels.
R-RNO-5578775. Ion homeostasis.
R-RNO-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ64568.

Gene expression databases

BgeeiENSRNOG00000017798.
ExpressionAtlasiQ64568. baseline.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT2B3_RAT
AccessioniPrimary (citable) accession number: Q64568
Secondary accession number(s): Q01489, Q63444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.