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Q64568

- AT2B3_RAT

UniProt

Q64568 - AT2B3_RAT

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Protein
Plasma membrane calcium-transporting ATPase 3
Gene
Atp2b3, Pmca3
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei473 – 47314-aspartylphosphate intermediate By similarity
Metal bindingi794 – 7941Magnesium By similarity
Metal bindingi798 – 7981Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. PDZ domain binding Source: RGD
  3. calcium-transporting ATPase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW
  5. protein binding Source: UniProtKB

GO - Biological processi

  1. brain development Source: RGD
  2. neural retina development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 3 (EC:3.6.3.8)
Short name:
PMCA3
Alternative name(s):
Plasma membrane calcium ATPase isoform 3
Plasma membrane calcium pump isoform 3
Gene namesi
Name:Atp2b3
Synonyms:Pmca3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi621304. Atp2b3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9797Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei98 – 11821Helical; Reviewed prediction
Add
BLAST
Topological domaini119 – 15537Extracellular Reviewed prediction
Add
BLAST
Transmembranei156 – 17621Helical; Reviewed prediction
Add
BLAST
Topological domaini177 – 364188Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei365 – 38420Helical; Reviewed prediction
Add
BLAST
Topological domaini385 – 41733Extracellular Reviewed prediction
Add
BLAST
Transmembranei418 – 43518Helical; Reviewed prediction
Add
BLAST
Topological domaini436 – 849414Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei850 – 86920Helical; Reviewed prediction
Add
BLAST
Topological domaini870 – 87910Extracellular Reviewed prediction
Transmembranei880 – 90021Helical; Reviewed prediction
Add
BLAST
Topological domaini901 – 92020Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei921 – 94323Helical; Reviewed prediction
Add
BLAST
Topological domaini944 – 96118Extracellular Reviewed prediction
Add
BLAST
Transmembranei962 – 98322Helical; Reviewed prediction
Add
BLAST
Topological domaini984 – 100219Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1003 – 102422Helical; Reviewed prediction
Add
BLAST
Topological domaini1025 – 103410Extracellular Reviewed prediction
Transmembranei1035 – 105622Helical; Reviewed prediction
Add
BLAST
Topological domaini1057 – 1258202Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane raft Source: RGD
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12581258Plasma membrane calcium-transporting ATPase 3
PRO_0000046219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1113 – 11131Phosphothreonine; by PKC By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64568.
PRIDEiQ64568.

PTM databases

PhosphoSiteiQ64568.

Expressioni

Tissue specificityi

Isoform XA is the most abundant form in brain and most other tissues. Isoform XB is the most abundant form in skeletal muscle and is also found in brain and at low levels in testis and kidney. The other isoforms are only found at low levels in various tissues.

Gene expression databases

ArrayExpressiQ64568.
GenevestigatoriQ64568.

Interactioni

Subunit structurei

Interacts with PDZD11 By similarity.

Protein-protein interaction databases

BioGridi248231. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64568.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1097 – 111418Calmodulin-binding subdomain A By similarity
Add
BLAST
Regioni1115 – 112410Calmodulin-binding subdomain B By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi297 – 3004Poly-Glu
Compositional biasi1212 – 12176Poly-Pro

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ64568.
KOiK05850.
PhylomeDBiQ64568.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (12)i

Sequence statusi: Complete.

This entry describes 12 isoformsi produced by alternative splicing. Align

Note: There is a combination of two alternative spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A, B, C, D, E and F). So far the splice sites have been studied independently. Experimental confirmation may be lacking for some isoforms.

Isoform XD (identifier: Q64568-1) [UniParc]FASTAAdd to Basket

Also known as: AIICIV

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGDMANSSIE FHPKPQQQRE VPHVGGFGCT LAELRSLMEL RGAEALQKIQ     50
EAYGDVSGLC RRLKTSPTEG LADNTNDLEK RRQIYGQNFI PPKQPKTFLQ 100
LVWEALQDVT LIILEVAAIV SLGLSFYAPP GEESEACGNV SGGAEDEGEA 150
EAGWIEGAAI LLSVICVVLV TAFNDWSKEK QFRGLQSRIE QEQKFTVIRN 200
GQLLQVPVAA LVVGDIAQVK YGDLLPADGV LIQGNDLKID ESSLTGESDH 250
VRKSADKDPM LLSGTHVMEG SGRMVVTAVG VNSQTGIIFT LLGAGGEEEE 300
KKDKKGKQQD GAMESSQTKA KKQDGAVAME MQPLKSAEGG EMEEREKKKA 350
NVPKKEKSVL QGKLTKLAVQ IGKAGLVMSA ITVIILVLYF VIETFVVDGR 400
VWLAECTPVY VQYFVKFFII GVTVLVVAVP EGLPLAVTIS LAYSVKKMMK 450
DNNLVRHLDA CETMGNATAI CSDKTGTLTT NRMTVVQSYL GDTHYKEIPA 500
PSALTPKILD LLVHAISINS AYTTKILPPE KEGALPRQVG NKTECALLGF 550
ILDLKRDFQP VREQIPEDQL YKVYTFNSVR KSMSTVIRMP DGGFRLFSKG 600
ASEILLKKCT NILNSNGELR GFRPRDRDDM VKKIIEPMAC DGLRTICIAY 650
RDFSAIQEPD WDNENEVVGD LTCIAVVGIE DPVRPEVPEA IRKCQRAGIT 700
VRMVTGDNIN TARAIAAKCG IIQPGEDFLC LEGKEFNRRI RNEKGEIEQE 750
RLDKVWPKLR VLARSSPTDK HTLVKGIIDS TTGEQRQVVA VTGDGTNDGP 800
ALKKADVGFA MGIAGTDVAK EASDIILTDD NFTSIVKAVM WGRNVYDSIS 850
KFLQFQLTVN VVAVIVAFTG ACITQDSPLK AVQMLWVNLI MDTFASLALA 900
TEPPTESLLL RKPYGRDKPL ISRTMMKNIL GHAVYQLTII FTLLFVGELF 950
FDIDSGRNAP LHSPPSEHYT IIFNTFVMMQ LFNEINARKI HGERNVFDGI 1000
FSNPIFCTIV LGTFGIQIVI VQFGGKPFSC SPLSTEQWLW CLFVGVGELV 1050
WGQVIATIPT SQLKCLKEAG HGPGKDEMTD EELAEGEEEI DHAERELRRG 1100
QILWFRGLNR IQTQMEVVST FKRSGSFQGA VRRRSSVLSQ LHDVTNLSTP 1150
THIRVVKAFR SSLYEGLEKP ESKSCIHNFM ATPEFLINDY THNIPLIDDT 1200
DVDENEERLR APPPPPPNQN NNAIDSGIYL TTHATKSATS SAFSSRPGSP 1250
LHSMETSL 1258
Length:1,258
Mass (Da):138,559
Last modified:June 1, 2001 - v2
Checksum:i3A13781D21042BA6
GO
Isoform XA (identifier: Q64568-2) [UniParc]FASTAAdd to Basket

Also known as: AIICII

The sequence of this isoform differs from the canonical sequence as follows:
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP

Show »
Length:1,173
Mass (Da):128,773
Checksum:iCF49538EC579E958
GO
Isoform ZA (identifier: Q64568-3) [UniParc]FASTAAdd to Basket

Also known as: AICII

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP

Show »
Length:1,159
Mass (Da):127,297
Checksum:iA33A52545101241D
GO
Isoform XB (identifier: Q64568-4) [UniParc]FASTAAdd to Basket

Also known as: AIICI

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1152: Missing.

Show »
Length:1,220
Mass (Da):134,360
Checksum:iC18A4BAEBA0362ED
GO
Isoform ZB (identifier: Q64568-5) [UniParc]FASTAAdd to Basket

Also known as: AICI

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1115-1152: Missing.

Show »
Length:1,206
Mass (Da):132,884
Checksum:iF83F2E1301CB4552
GO
Isoform XC (identifier: Q64568-6) [UniParc]FASTAAdd to Basket

Also known as: AIICIII

The sequence of this isoform differs from the canonical sequence as follows:
     1144-1152: Missing.

Show »
Length:1,249
Mass (Da):137,608
Checksum:i9825517A8B9744B1
GO
Isoform ZC (identifier: Q64568-7) [UniParc]FASTAAdd to Basket

Also known as: AICIII

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1144-1152: Missing.

Show »
Length:1,235
Mass (Da):136,132
Checksum:iDD7B43D805121FDD
GO
Isoform ZD (identifier: Q64568-8) [UniParc]FASTAAdd to Basket

Also known as: AICIV

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.

Show »
Length:1,244
Mass (Da):137,083
Checksum:i3BA47725ACB4D4AE
GO
Isoform XE (identifier: Q64568-9) [UniParc]FASTAAdd to Basket

Also known as: AIICV

The sequence of this isoform differs from the canonical sequence as follows:
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES

Show »
Length:1,168
Mass (Da):128,295
Checksum:i09F539391396F9A2
GO
Isoform ZE (identifier: Q64568-10) [UniParc]FASTAAdd to Basket

Also known as: AICV

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES

Show »
Length:1,154
Mass (Da):126,818
Checksum:i81387C79448BCC0F
GO
Isoform XF (identifier: Q64568-11) [UniParc]FASTAAdd to Basket

Also known as: AIICVI

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

Show »
Length:1,129
Mass (Da):124,342
Checksum:i7B4F423A0C709026
GO
Isoform ZF (identifier: Q64568-12) [UniParc]FASTAAdd to Basket

Also known as: AICVI

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

Show »
Length:1,115
Mass (Da):122,866
Checksum:i1A545F41D1A951EB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei306 – 31914Missing in isoform ZA, isoform ZB, isoform ZC, isoform ZD, isoform ZE and isoform ZF.
VSP_000396Add
BLAST
Alternative sequencei1115 – 1258144MEVVS…METSL → VCWDGKKMLRTTEVG in isoform XF and isoform ZF.
VSP_000398Add
BLAST
Alternative sequencei1115 – 115238Missing in isoform XB and isoform ZB.
VSP_000397Add
BLAST
Alternative sequencei1144 – 11529Missing in isoform XC and isoform ZC.
VSP_000399
Alternative sequencei1153 – 1258106IRVVK…METSL → VTLSAAKPTSAAGNPSGESI P in isoform XA and isoform ZA.
VSP_000400Add
BLAST
Alternative sequencei1153 – 1258106IRVVK…METSL → VTLSAAKPTSAAGSES in isoform XE and isoform ZE.
VSP_000401Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05565, L05558, L05564 Genomic DNA. Translation: AAA53650.1.
J05087 mRNA. Translation: AAA69667.1.
M96626 mRNA. Translation: AAA50821.1.
PIRiA34308.
C44525.
RefSeqiNP_579822.1. NM_133288.1. [Q64568-3]
UniGeneiRn.11053.

Genome annotation databases

EnsembliENSRNOT00000023934; ENSRNOP00000023934; ENSRNOG00000017798. [Q64568-3]
GeneIDi29599.
KEGGirno:29599.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05565 , L05558 , L05564 Genomic DNA. Translation: AAA53650.1 .
J05087 mRNA. Translation: AAA69667.1 .
M96626 mRNA. Translation: AAA50821.1 .
PIRi A34308.
C44525.
RefSeqi NP_579822.1. NM_133288.1. [Q64568-3 ]
UniGenei Rn.11053.

3D structure databases

ProteinModelPortali Q64568.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248231. 1 interaction.

PTM databases

PhosphoSitei Q64568.

Proteomic databases

PaxDbi Q64568.
PRIDEi Q64568.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000023934 ; ENSRNOP00000023934 ; ENSRNOG00000017798 . [Q64568-3 ]
GeneIDi 29599.
KEGGi rno:29599.

Organism-specific databases

CTDi 492.
RGDi 621304. Atp2b3.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00510000046331.
HOGENOMi HOG000265623.
HOVERGENi HBG061286.
InParanoidi Q64568.
KOi K05850.
PhylomeDBi Q64568.

Miscellaneous databases

NextBioi 609754.
PROi Q64568.

Gene expression databases

ArrayExpressi Q64568.
Genevestigatori Q64568.

Family and domain databases

Gene3Di 1.20.1110.10. 3 hits.
InterProi IPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a third isoform of the calmodulin-sensitive plasma membrane Ca2+-transporting ATPase that is expressed predominantly in brain and skeletal muscle."
    Greeb J., Shull G.E.
    J. Biol. Chem. 264:18569-18576(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ZA).
    Tissue: Brain.
  2. "Structure of the rat plasma membrane Ca(2+)-ATPase isoform 3 gene and characterization of alternative splicing and transcription products. Skeletal muscle-specific splicing results in a plasma membrane Ca(2+)-ATPase with a novel calmodulin-binding domain."
    Burk S.E., Shull G.E.
    J. Biol. Chem. 267:19683-19690(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1016-1258 (ISOFORMS XF/ZF), ALTERNATIVE SPLICING.
    Strain: CD Charles River.
  3. "Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
    Keeton T.P., Burk S.E., Shull G.E.
    J. Biol. Chem. 268:2740-2748(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1054-1258, ALTERNATIVE SPLICING (ISOFORMS XA/ZA).
    Strain: CD Charles River.

Entry informationi

Entry nameiAT2B3_RAT
AccessioniPrimary (citable) accession number: Q64568
Secondary accession number(s): Q01489, Q63444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi