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Q64566 (AT2C1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-transporting ATPase type 2C member 1

Short name=ATPase 2C1
EC=3.6.3.8
Alternative name(s):
ATP-dependent Ca(2+) pump PMR1
Gene names
Name:Atp2c1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length919 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium By similarity.

Catalytic activity

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Ubiquitously expressed. The long isoform isonly found in testis. Ref.1

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. [View classification]

Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentGolgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Calcium
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi calcium ion homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

Golgi calcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

actin cytoskeleton reorganization

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion import

Inferred by curator PubMed 19527224. Source: RGD

calcium ion transmembrane transport

Inferred from sequence or structural similarity. Source: GOC

calcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-dependent cell-cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

cellular calcium ion homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

cellular manganese ion homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

epidermis development

Inferred from sequence or structural similarity. Source: UniProtKB

manganese ion transmembrane transport

Inferred from sequence or structural similarity. Source: GOC

manganese ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from sequence or structural similarity. Source: UniProtKB

signal transduction

Inferred from sequence or structural similarity. Source: GOC

   Cellular_componentGolgi apparatus

Inferred from sequence or structural similarity. Source: UniProtKB

Golgi membrane

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 14747290PubMed 19527224. Source: RGD

plasma membrane

Inferred from direct assay PubMed 14747290. Source: RGD

secretory granule

Inferred from direct assay PubMed 14747290. Source: RGD

trans-Golgi network

Inferred from sequence or structural similarity. Source: UniProtKB

transport vesicle

Inferred from direct assay PubMed 14747290. Source: RGD

   Molecular_functionATP binding

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-transporting ATPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

manganese ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

manganese-transporting ATPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

signal transducer activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Short (identifier: Q64566-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Long (identifier: Q64566-2)

The sequence of this isoform differs from the canonical sequence as follows:
     919-919: V → FYPKI

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 919919Calcium-transporting ATPase type 2C member 1
PRO_0000046225

Regions

Topological domain1 – 7878Cytoplasmic Potential
Transmembrane79 – 9517Helical; Potential
Topological domain96 – 994Extracellular Potential
Transmembrane100 – 12122Helical; Potential
Topological domain122 – 263142Cytoplasmic Potential
Transmembrane264 – 28320Helical; Potential
Topological domain284 – 29512Extracellular Potential
Transmembrane296 – 31722Helical; Potential
Topological domain318 – 700383Cytoplasmic Potential
Transmembrane701 – 72323Helical; Potential
Topological domain724 – 7285Extracellular Potential
Transmembrane729 – 75224Helical; Potential
Topological domain753 – 77624Cytoplasmic Potential
Transmembrane777 – 79519Helical; Potential
Topological domain796 – 8027Extracellular Potential
Transmembrane803 – 82826Helical; Potential
Topological domain829 – 84315Cytoplasmic Potential
Transmembrane844 – 86320Helical; Potential
Topological domain864 – 87613Extracellular Potential
Transmembrane877 – 89317Helical; Potential
Topological domain894 – 91926Cytoplasmic Potential

Sites

Active site35014-aspartylphosphate intermediate By similarity
Metal binding6441Magnesium By similarity
Metal binding6481Magnesium By similarity

Natural variations

Alternative sequence9191V → FYPKI in isoform Long.
VSP_000411

Experimental info

Sequence conflict5381L → F in AAA73342. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Short [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D26C8C95C14DFB8A

FASTA919100,500
        10         20         30         40         50         60 
MKVARFQKIP NVENETMIPV LTSKRASELA VSEVAGLLQA DLQNGLNKSE VSHRRAFHGW 

        70         80         90        100        110        120 
NEFDISEDEP LWKKYISQFK NPLIMLLLAS AVISVLMRQF DDAVSITVAI LIVVTVAFVQ 

       130        140        150        160        170        180 
EYRSEKSLEE LSKLVPPECH CVREGKLEHT LARDLVPGDT VCLSVGDRVP ADLRLFEAVD 

       190        200        210        220        230        240 
LSIDESSLTG ETTPCSKVTA PQPAATNGDL ASRSNIAFMG TLVRCGKAKG IVIGTGENSE 

       250        260        270        280        290        300 
FGEVFKMMQA EEAPKTPLQK SMDLLGKQLS FYSFGIIGII MLVGWLLGKD ILEMFTISVS 

       310        320        330        340        350        360 
LAVAAIPEGL PIVVTVTLAL GVMRMVKKRA IVKKLPIVET LGCCNVICSD KTGTLTKNEM 

       370        380        390        400        410        420 
TVTHILTSDG LHAEVTGVGY NQFGEVIVDG DVVHGFYNPA VSRIVEAGCV CNDAVIRNNT 

       430        440        450        460        470        480 
LMGKPTEGAL IALAMKMGLD GLQQDYIRKA EYPFSSEQKW MAVKCVHRTQ QDRPEICFMK 

       490        500        510        520        530        540 
GAYEQVIKYC TTYNSKGQTL ALTQQQRDLY QQEKAQMGSA GLRVLALASG PDLGQLTLLG 

       550        560        570        580        590        600 
LVGIIDPPRT GVKEAVTTLI ASGVSIKMIT GDSQETAIAI ASRLGLYSKT SQSVSGEEVD 

       610        620        630        640        650        660 
TMEVQHLSQI VPKVAVFYRA SPRHKMKIIK SLQKNGSVVA MTGDGVNDAV ALKAADIGVA 

       670        680        690        700        710        720 
MGQTGTDVCK EAADMILVDD DFQTIMSAIE EGKGIYNNIK NFVRFQLSTS IAALTLISLA 

       730        740        750        760        770        780 
TLMNFPNPLN AMQILWINII MDGPPAQSLG VEPVDKDVIR KPPRNWKDSI LTKNLILKIL 

       790        800        810        820        830        840 
VSSIIIVCGT LFVFWRELRD NVITPRDTTM TFTCFVFFDM FNALSSRSQT KSVFEIGLCS 

       850        860        870        880        890        900 
NKMFCYAVLG SIMGQLLVIY FPPLQKVFQT ESLSILDLLF LLGLTSSVCI VSEIIKKVER 

       910 
SREKTQKNTT STPSSFLEV 

« Hide

Isoform Long [UniParc].

Checksum: D58541FD196C8C95
Show »

FASTA923101,050

References

[1]"Molecular cloning and tissue distribution of alternatively spliced mRNAs encoding possible mammalian homologues of the yeast secretory pathway calcium pump."
Gunteski-Hamblin A.-M., Clarke D.M., Shull G.E.
Biochemistry 31:7600-7608(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Stomach and Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M93017 mRNA. Translation: AAA73341.1.
M93018 mRNA. Translation: AAA73342.1.
PIRA42764.
UniGeneRn.5805.

3D structure databases

ProteinModelPortalQ64566.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4568586.
STRING10116.ENSRNOP00000018175.

Proteomic databases

PaxDbQ64566.
PRIDEQ64566.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:621311. rat. [Q64566-1]

Organism-specific databases

RGD621311. Atp2c1.

Phylogenomic databases

eggNOGCOG0474.
HOGENOMHOG000265621.
HOVERGENHBG106478.
PhylomeDBQ64566.

Gene expression databases

GenevestigatorQ64566.

Family and domain databases

Gene3D1.20.1110.10. 3 hits.
InterProIPR006413. ATPase_P-typ_Ca-transp_PMR1.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01522. ATPase-IIA2_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ64566.

Entry information

Entry nameAT2C1_RAT
AccessionPrimary (citable) accession number: Q64566
Secondary accession number(s): Q64567
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families