Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-transporting ATPase type 2C member 1

Gene

Atp2c1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei350 – 35014-aspartylphosphate intermediateBy similarity
Metal bindingi644 – 6441MagnesiumBy similarity
Metal bindingi648 – 6481MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. calcium ion binding Source: UniProtKB
  3. calcium-transporting ATPase activity Source: UniProtKB
  4. manganese ion binding Source: UniProtKB
  5. manganese-transporting ATPase activity Source: UniProtKB
  6. signal transducer activity Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton reorganization Source: UniProtKB
  2. calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
  3. calcium ion import Source: RGD
  4. calcium ion transmembrane transport Source: GOC
  5. calcium ion transport Source: UniProtKB
  6. cellular calcium ion homeostasis Source: UniProtKB
  7. cellular manganese ion homeostasis Source: UniProtKB
  8. epidermis development Source: UniProtKB
  9. Golgi calcium ion homeostasis Source: UniProtKB
  10. Golgi calcium ion transport Source: UniProtKB
  11. manganese ion transmembrane transport Source: GOC
  12. manganese ion transport Source: UniProtKB
  13. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  14. signal transduction Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase type 2C member 1 (EC:3.6.3.8)
Short name:
ATPase 2C1
Alternative name(s):
ATP-dependent Ca(2+) pump PMR1
Gene namesi
Name:Atp2c1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621311. Atp2c1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7878CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei79 – 9517HelicalSequence AnalysisAdd
BLAST
Topological domaini96 – 994ExtracellularSequence Analysis
Transmembranei100 – 12122HelicalSequence AnalysisAdd
BLAST
Topological domaini122 – 263142CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei264 – 28320HelicalSequence AnalysisAdd
BLAST
Topological domaini284 – 29512ExtracellularSequence AnalysisAdd
BLAST
Transmembranei296 – 31722HelicalSequence AnalysisAdd
BLAST
Topological domaini318 – 700383CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei701 – 72323HelicalSequence AnalysisAdd
BLAST
Topological domaini724 – 7285ExtracellularSequence Analysis
Transmembranei729 – 75224HelicalSequence AnalysisAdd
BLAST
Topological domaini753 – 77624CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei777 – 79519HelicalSequence AnalysisAdd
BLAST
Topological domaini796 – 8027ExtracellularSequence Analysis
Transmembranei803 – 82826HelicalSequence AnalysisAdd
BLAST
Topological domaini829 – 84315CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei844 – 86320HelicalSequence AnalysisAdd
BLAST
Topological domaini864 – 87613ExtracellularSequence AnalysisAdd
BLAST
Transmembranei877 – 89317HelicalSequence AnalysisAdd
BLAST
Topological domaini894 – 91926CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. Golgi membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: RGD
  5. plasma membrane Source: RGD
  6. secretory granule Source: RGD
  7. trans-Golgi network Source: UniProtKB
  8. transport vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Calcium-transporting ATPase type 2C member 1PRO_0000046225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei235 – 2351PhosphothreonineBy similarity
Modified residuei239 – 2391PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64566.
PRIDEiQ64566.

Expressioni

Tissue specificityi

Ubiquitously expressed. The long isoform is only found in testis.1 Publication

Gene expression databases

GenevestigatoriQ64566.

Interactioni

Protein-protein interaction databases

MINTiMINT-4568586.
STRINGi10116.ENSRNOP00000018175.

Structurei

3D structure databases

ProteinModelPortaliQ64566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265621.
HOVERGENiHBG106478.
InParanoidiQ64566.
PhylomeDBiQ64566.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR030336. ATP2C1.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006413. P-type_ATPase_IIA_PMR1.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF214. PTHR24093:SF214. 1 hit.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01522. ATPase-IIA2_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Short (identifier: Q64566-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVARFQKIP NVENETMIPV LTSKRASELA VSEVAGLLQA DLQNGLNKSE
60 70 80 90 100
VSHRRAFHGW NEFDISEDEP LWKKYISQFK NPLIMLLLAS AVISVLMRQF
110 120 130 140 150
DDAVSITVAI LIVVTVAFVQ EYRSEKSLEE LSKLVPPECH CVREGKLEHT
160 170 180 190 200
LARDLVPGDT VCLSVGDRVP ADLRLFEAVD LSIDESSLTG ETTPCSKVTA
210 220 230 240 250
PQPAATNGDL ASRSNIAFMG TLVRCGKAKG IVIGTGENSE FGEVFKMMQA
260 270 280 290 300
EEAPKTPLQK SMDLLGKQLS FYSFGIIGII MLVGWLLGKD ILEMFTISVS
310 320 330 340 350
LAVAAIPEGL PIVVTVTLAL GVMRMVKKRA IVKKLPIVET LGCCNVICSD
360 370 380 390 400
KTGTLTKNEM TVTHILTSDG LHAEVTGVGY NQFGEVIVDG DVVHGFYNPA
410 420 430 440 450
VSRIVEAGCV CNDAVIRNNT LMGKPTEGAL IALAMKMGLD GLQQDYIRKA
460 470 480 490 500
EYPFSSEQKW MAVKCVHRTQ QDRPEICFMK GAYEQVIKYC TTYNSKGQTL
510 520 530 540 550
ALTQQQRDLY QQEKAQMGSA GLRVLALASG PDLGQLTLLG LVGIIDPPRT
560 570 580 590 600
GVKEAVTTLI ASGVSIKMIT GDSQETAIAI ASRLGLYSKT SQSVSGEEVD
610 620 630 640 650
TMEVQHLSQI VPKVAVFYRA SPRHKMKIIK SLQKNGSVVA MTGDGVNDAV
660 670 680 690 700
ALKAADIGVA MGQTGTDVCK EAADMILVDD DFQTIMSAIE EGKGIYNNIK
710 720 730 740 750
NFVRFQLSTS IAALTLISLA TLMNFPNPLN AMQILWINII MDGPPAQSLG
760 770 780 790 800
VEPVDKDVIR KPPRNWKDSI LTKNLILKIL VSSIIIVCGT LFVFWRELRD
810 820 830 840 850
NVITPRDTTM TFTCFVFFDM FNALSSRSQT KSVFEIGLCS NKMFCYAVLG
860 870 880 890 900
SIMGQLLVIY FPPLQKVFQT ESLSILDLLF LLGLTSSVCI VSEIIKKVER
910
SREKTQKNTT STPSSFLEV
Length:919
Mass (Da):100,500
Last modified:November 1, 1996 - v1
Checksum:iD26C8C95C14DFB8A
GO
Isoform Long (identifier: Q64566-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     919-919: V → FYPKI

Show »
Length:923
Mass (Da):101,050
Checksum:iD58541FD196C8C95
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti538 – 5381L → F in AAA73342 (PubMed:1380825).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei919 – 9191V → FYPKI in isoform Long. 1 PublicationVSP_000411

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93017 mRNA. Translation: AAA73341.1.
M93018 mRNA. Translation: AAA73342.1.
PIRiA42764.
UniGeneiRn.5805.

Genome annotation databases

UCSCiRGD:621311. rat. [Q64566-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93017 mRNA. Translation: AAA73341.1.
M93018 mRNA. Translation: AAA73342.1.
PIRiA42764.
UniGeneiRn.5805.

3D structure databases

ProteinModelPortaliQ64566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4568586.
STRINGi10116.ENSRNOP00000018175.

Proteomic databases

PaxDbiQ64566.
PRIDEiQ64566.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621311. rat. [Q64566-1]

Organism-specific databases

RGDi621311. Atp2c1.

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265621.
HOVERGENiHBG106478.
InParanoidiQ64566.
PhylomeDBiQ64566.

Miscellaneous databases

PROiQ64566.

Gene expression databases

GenevestigatoriQ64566.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR030336. ATP2C1.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006413. P-type_ATPase_IIA_PMR1.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF214. PTHR24093:SF214. 1 hit.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01522. ATPase-IIA2_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and tissue distribution of alternatively spliced mRNAs encoding possible mammalian homologues of the yeast secretory pathway calcium pump."
    Gunteski-Hamblin A.-M., Clarke D.M., Shull G.E.
    Biochemistry 31:7600-7608(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Stomach and Testis.

Entry informationi

Entry nameiAT2C1_RAT
AccessioniPrimary (citable) accession number: Q64566
Secondary accession number(s): Q64567
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: March 4, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.