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Q64562

- CP21A_RAT

UniProt

Q64562 - CP21A_RAT

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Protein
Steroid 21-hydroxylase
Gene
Cyp21, Cyp21a1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activityi

A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi423 – 4231Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. steroid 21-monooxygenase activity Source: RGD
  4. steroid binding Source: UniProtKB-KW

GO - Biological processi

  1. C21-steroid hormone biosynthetic process Source: RGD
  2. cellular response to peptide hormone stimulus Source: RGD
  3. mineralocorticoid biosynthetic process Source: RGD
  4. progesterone metabolic process Source: RGD
  5. response to antibiotic Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.99.10)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:Cyp21
Synonyms:Cyp21a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2461. Cyp21a1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Steroid 21-hydroxylase
PRO_0000051979Add
BLAST

Proteomic databases

PaxDbiQ64562.
PRIDEiQ64562.

Expressioni

Gene expression databases

GenevestigatoriQ64562.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000037516.

Structurei

3D structure databases

ProteinModelPortaliQ64562.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni337 – 35317Steroid-binding By similarity
Add
BLAST

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiQ64562.
PhylomeDBiQ64562.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64562-1 [UniParc]FASTAAdd to Basket

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MLLPGLLLLL LLLLLAGTRW LWGQWKLWKL RLPPLAPGFL HFLQPNLPVY    50
LFGLAQKLGP IYRIRLGLQD VVVLNSNKTI EEALIQKWVD FAGRPQILDG 100
KMNFDLSMGD YSLTWKAHKK LSRSALVLGM RDSMEPLVEQ LTQEFCERMR 150
AQAGASVAIH KEFSLLTCSI ISCLTFGDKQ DSTLLNATHS CVRDLLKAWN 200
HWSVQILDII PFLRFFPNPG LWKLKQFQES RDHIVMQELK RHKDSLVAGQ 250
WKDMIDYMLQ GVEKQRDARD PGQLHERHVH MSVVDLFVGG TETTAATLSW 300
AVAFLLHHPE IQKRLQEELD LKLAPSSQLL YKNRMQLPLL MATIAEVLRL 350
RPVVPMALPH RATKASSISG YDIPKDTIII PNIQGANLDE MVWELPSKFW 400
PDRFLESGKS PRIPTFGCGA RVCLGEPLAR LEFFVVLARL LQTFTLLPPP 450
DGTLPSLQPL PYTGINLLIP PFQVRLQPRN LAPQDQGQKS STG 493
Length:493
Mass (Da):55,946
Last modified:November 1, 1996 - v1
Checksum:iFF6CC5E993B8B133
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U56853 mRNA. Translation: AAD05573.1.
UniGeneiRn.161726.

Genome annotation databases

UCSCiRGD:2461. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U56853 mRNA. Translation: AAD05573.1 .
UniGenei Rn.161726.

3D structure databases

ProteinModelPortali Q64562.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000037516.

Proteomic databases

PaxDbi Q64562.
PRIDEi Q64562.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2461. rat.

Organism-specific databases

RGDi 2461. Cyp21a1.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000036991.
HOVERGENi HBG106944.
InParanoidi Q64562.
PhylomeDBi Q64562.

Gene expression databases

Genevestigatori Q64562.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of two alternatively spliced 21-hydroxylase cDNAs from rat adrenal."
    Zhou M.Y., del Carmen Vila M., Gomez-Sanchez E.P., Gomez-Sanchez C.E.
    J. Steroid Biochem. Mol. Biol. 62:277-286(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiCP21A_RAT
AccessioniPrimary (citable) accession number: Q64562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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