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Q64562 (CP21A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 21-hydroxylase

EC=1.14.99.10
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene names
Name:Cyp21
Synonyms:Cyp21a1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activity

A C21 steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Domain

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Steroid 21-hydroxylase
PRO_0000051979

Regions

Region337 – 35317Steroid-binding By similarity

Sites

Metal binding4231Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64562 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FF6CC5E993B8B133

FASTA49355,946
        10         20         30         40         50         60 
MLLPGLLLLL LLLLLAGTRW LWGQWKLWKL RLPPLAPGFL HFLQPNLPVY LFGLAQKLGP 

        70         80         90        100        110        120 
IYRIRLGLQD VVVLNSNKTI EEALIQKWVD FAGRPQILDG KMNFDLSMGD YSLTWKAHKK 

       130        140        150        160        170        180 
LSRSALVLGM RDSMEPLVEQ LTQEFCERMR AQAGASVAIH KEFSLLTCSI ISCLTFGDKQ 

       190        200        210        220        230        240 
DSTLLNATHS CVRDLLKAWN HWSVQILDII PFLRFFPNPG LWKLKQFQES RDHIVMQELK 

       250        260        270        280        290        300 
RHKDSLVAGQ WKDMIDYMLQ GVEKQRDARD PGQLHERHVH MSVVDLFVGG TETTAATLSW 

       310        320        330        340        350        360 
AVAFLLHHPE IQKRLQEELD LKLAPSSQLL YKNRMQLPLL MATIAEVLRL RPVVPMALPH 

       370        380        390        400        410        420 
RATKASSISG YDIPKDTIII PNIQGANLDE MVWELPSKFW PDRFLESGKS PRIPTFGCGA 

       430        440        450        460        470        480 
RVCLGEPLAR LEFFVVLARL LQTFTLLPPP DGTLPSLQPL PYTGINLLIP PFQVRLQPRN 

       490 
LAPQDQGQKS STG 

« Hide

References

[1]"Cloning of two alternatively spliced 21-hydroxylase cDNAs from rat adrenal."
Zhou M.Y., del Carmen Vila M., Gomez-Sanchez E.P., Gomez-Sanchez C.E.
J. Steroid Biochem. Mol. Biol. 62:277-286(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U56853 mRNA. Translation: AAD05573.1.
UniGeneRn.161726.

3D structure databases

ProteinModelPortalQ64562.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000037516.

Proteomic databases

PaxDbQ64562.
PRIDEQ64562.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:2461. rat.

Organism-specific databases

RGD2461. Cyp21a1.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG106944.
InParanoidQ64562.
PhylomeDBQ64562.

Gene expression databases

GenevestigatorQ64562.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP21A_RAT
AccessionPrimary (citable) accession number: Q64562
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families