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Protein

Steroid 21-hydroxylase

Gene

Cyp21

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activityi

A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi423 – 4231Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • steroid 21-monooxygenase activity Source: RGD
  • steroid binding Source: UniProtKB-KW

GO - Biological processi

  • C21-steroid hormone biosynthetic process Source: RGD
  • cellular response to peptide hormone stimulus Source: RGD
  • mineralocorticoid biosynthetic process Source: RGD
  • progesterone metabolic process Source: RGD
  • response to antibiotic Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.99.10)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:Cyp21
Synonyms:Cyp21a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2461. Cyp21a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Steroid 21-hydroxylasePRO_0000051979Add
BLAST

Proteomic databases

PaxDbiQ64562.
PRIDEiQ64562.

Expressioni

Gene expression databases

GenevestigatoriQ64562.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000037516.

Structurei

3D structure databases

ProteinModelPortaliQ64562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni337 – 35317Steroid-bindingBy similarityAdd
BLAST

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiQ64562.
PhylomeDBiQ64562.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPGLLLLL LLLLLAGTRW LWGQWKLWKL RLPPLAPGFL HFLQPNLPVY
60 70 80 90 100
LFGLAQKLGP IYRIRLGLQD VVVLNSNKTI EEALIQKWVD FAGRPQILDG
110 120 130 140 150
KMNFDLSMGD YSLTWKAHKK LSRSALVLGM RDSMEPLVEQ LTQEFCERMR
160 170 180 190 200
AQAGASVAIH KEFSLLTCSI ISCLTFGDKQ DSTLLNATHS CVRDLLKAWN
210 220 230 240 250
HWSVQILDII PFLRFFPNPG LWKLKQFQES RDHIVMQELK RHKDSLVAGQ
260 270 280 290 300
WKDMIDYMLQ GVEKQRDARD PGQLHERHVH MSVVDLFVGG TETTAATLSW
310 320 330 340 350
AVAFLLHHPE IQKRLQEELD LKLAPSSQLL YKNRMQLPLL MATIAEVLRL
360 370 380 390 400
RPVVPMALPH RATKASSISG YDIPKDTIII PNIQGANLDE MVWELPSKFW
410 420 430 440 450
PDRFLESGKS PRIPTFGCGA RVCLGEPLAR LEFFVVLARL LQTFTLLPPP
460 470 480 490
DGTLPSLQPL PYTGINLLIP PFQVRLQPRN LAPQDQGQKS STG
Length:493
Mass (Da):55,946
Last modified:November 1, 1996 - v1
Checksum:iFF6CC5E993B8B133
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56853 mRNA. Translation: AAD05573.1.
UniGeneiRn.161726.

Genome annotation databases

UCSCiRGD:2461. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56853 mRNA. Translation: AAD05573.1.
UniGeneiRn.161726.

3D structure databases

ProteinModelPortaliQ64562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000037516.

Proteomic databases

PaxDbiQ64562.
PRIDEiQ64562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2461. rat.

Organism-specific databases

RGDi2461. Cyp21a1.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiQ64562.
PhylomeDBiQ64562.

Miscellaneous databases

PROiQ64562.

Gene expression databases

GenevestigatoriQ64562.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of two alternatively spliced 21-hydroxylase cDNAs from rat adrenal."
    Zhou M.Y., del Carmen Vila M., Gomez-Sanchez E.P., Gomez-Sanchez C.E.
    J. Steroid Biochem. Mol. Biol. 62:277-286(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiCP21A_RAT
AccessioniPrimary (citable) accession number: Q64562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.