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Q64562

- CP21A_RAT

UniProt

Q64562 - CP21A_RAT

Protein

Steroid 21-hydroxylase

Gene

Cyp21

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

    Catalytic activityi

    A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi423 – 4231Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. steroid 21-monooxygenase activity Source: RGD
    4. steroid binding Source: UniProtKB-KW

    GO - Biological processi

    1. C21-steroid hormone biosynthetic process Source: RGD
    2. cellular response to peptide hormone stimulus Source: RGD
    3. mineralocorticoid biosynthetic process Source: RGD
    4. progesterone metabolic process Source: RGD
    5. response to antibiotic Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 21-hydroxylase (EC:1.14.99.10)
    Alternative name(s):
    21-OHase
    Cytochrome P-450c21
    Cytochrome P450 21
    Cytochrome P450 XXI
    Cytochrome P450-C21
    Gene namesi
    Name:Cyp21
    Synonyms:Cyp21a1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2461. Cyp21a1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493Steroid 21-hydroxylasePRO_0000051979Add
    BLAST

    Proteomic databases

    PaxDbiQ64562.
    PRIDEiQ64562.

    Expressioni

    Gene expression databases

    GenevestigatoriQ64562.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000037516.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64562.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni337 – 35317Steroid-bindingBy similarityAdd
    BLAST

    Domaini

    The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiQ64562.
    PhylomeDBiQ64562.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64562-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLPGLLLLL LLLLLAGTRW LWGQWKLWKL RLPPLAPGFL HFLQPNLPVY    50
    LFGLAQKLGP IYRIRLGLQD VVVLNSNKTI EEALIQKWVD FAGRPQILDG 100
    KMNFDLSMGD YSLTWKAHKK LSRSALVLGM RDSMEPLVEQ LTQEFCERMR 150
    AQAGASVAIH KEFSLLTCSI ISCLTFGDKQ DSTLLNATHS CVRDLLKAWN 200
    HWSVQILDII PFLRFFPNPG LWKLKQFQES RDHIVMQELK RHKDSLVAGQ 250
    WKDMIDYMLQ GVEKQRDARD PGQLHERHVH MSVVDLFVGG TETTAATLSW 300
    AVAFLLHHPE IQKRLQEELD LKLAPSSQLL YKNRMQLPLL MATIAEVLRL 350
    RPVVPMALPH RATKASSISG YDIPKDTIII PNIQGANLDE MVWELPSKFW 400
    PDRFLESGKS PRIPTFGCGA RVCLGEPLAR LEFFVVLARL LQTFTLLPPP 450
    DGTLPSLQPL PYTGINLLIP PFQVRLQPRN LAPQDQGQKS STG 493
    Length:493
    Mass (Da):55,946
    Last modified:November 1, 1996 - v1
    Checksum:iFF6CC5E993B8B133
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56853 mRNA. Translation: AAD05573.1.
    UniGeneiRn.161726.

    Genome annotation databases

    UCSCiRGD:2461. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56853 mRNA. Translation: AAD05573.1 .
    UniGenei Rn.161726.

    3D structure databases

    ProteinModelPortali Q64562.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000037516.

    Proteomic databases

    PaxDbi Q64562.
    PRIDEi Q64562.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2461. rat.

    Organism-specific databases

    RGDi 2461. Cyp21a1.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi Q64562.
    PhylomeDBi Q64562.

    Gene expression databases

    Genevestigatori Q64562.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of two alternatively spliced 21-hydroxylase cDNAs from rat adrenal."
      Zhou M.Y., del Carmen Vila M., Gomez-Sanchez E.P., Gomez-Sanchez C.E.
      J. Steroid Biochem. Mol. Biol. 62:277-286(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiCP21A_RAT
    AccessioniPrimary (citable) accession number: Q64562
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3