Reviewed, UniProtKB/Swiss-Prot Q64562 (CP21A_RAT)

Names and origin

Protein names

Recommended name:
    Steroid 21-hydroxylase
    EC=1.14.99.10
Alternative name(s):
    Cytochrome P450 21
    Cytochrome P450 XXI
    21-OHase
    Cytochrome P450-C21
    Cytochrome P-450c21

Gene names

Name:	Cyp21
Synonyms:	Cyp21a1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.
Catalytic activity	A steroid + AH2 + O2 = a 21-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Domain	The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Lipid-binding Metal-binding Steroid-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Ref.1 Inferred from mutant phenotype. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 21-monooxygenase activity Ref.1 Inferred from direct assay. Source: RGD steroid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Steroid 21-hydroxylase		PRO_0000051979
Regions
	Region	337 – 353	17	Steroid-binding By similarity
Sites
	Metal binding	423	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64562-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FF6CC5E993B8B133	FASTA	493	55,946
	10 20 30 40 50 60 MLLPGLLLLL LLLLLAGTRW LWGQWKLWKL RLPPLAPGFL HFLQPNLPVY LFGLAQKLGP 70 80 90 100 110 120 IYRIRLGLQD VVVLNSNKTI EEALIQKWVD FAGRPQILDG KMNFDLSMGD YSLTWKAHKK 130 140 150 160 170 180 LSRSALVLGM RDSMEPLVEQ LTQEFCERMR AQAGASVAIH KEFSLLTCSI ISCLTFGDKQ 190 200 210 220 230 240 DSTLLNATHS CVRDLLKAWN HWSVQILDII PFLRFFPNPG LWKLKQFQES RDHIVMQELK 250 260 270 280 290 300 RHKDSLVAGQ WKDMIDYMLQ GVEKQRDARD PGQLHERHVH MSVVDLFVGG TETTAATLSW 310 320 330 340 350 360 AVAFLLHHPE IQKRLQEELD LKLAPSSQLL YKNRMQLPLL MATIAEVLRL RPVVPMALPH 370 380 390 400 410 420 RATKASSISG YDIPKDTIII PNIQGANLDE MVWELPSKFW PDRFLESGKS PRIPTFGCGA 430 440 450 460 470 480 RVCLGEPLAR LEFFVVLARL LQTFTLLPPP DGTLPSLQPL PYTGINLLIP PFQVRLQPRN 490 LAPQDQGQKS STG

References

[1]	"Cloning of two alternatively spliced 21-hydroxylase cDNAs from rat adrenal." Zhou M.Y., del Carmen Vila M., Gomez-Sanchez E.P., Gomez-Sanchez C.E. J. Steroid Biochem. Mol. Biol. 62:277-286(1997) [PubMed: 9408081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U56853 mRNA. Translation: AAD05573.1.
IPI	IPI00213581.
UniGene	Rn.161726
3D structure databases
SMR	Q64562. Positions 46-479.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64562.
Proteomic databases
PRIDE	Q64562.
Genome annotation databases
Ensembl	ENSRNOT00000031548; ENSRNOP00000037516; ENSRNOG00000000428; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	2461. Cyp21a1.
Phylogenomic databases
eggNOG	roNOG08861.
HOVERGEN	Q64562.
InParanoid	Q64562.
Enzyme and pathway databases
BRENDA	1.14.99.10. 248.
Gene expression databases
ArrayExpress	Q64562.
Genevestigator	Q64562.
GermOnline	ENSRNOG00000000428. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602910.

Entry information

Entry name

CP21A_RAT

Accession

Primary (citable) accession number: Q64562

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)