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Protein

Tripeptidyl-peptidase 2

Gene

Tpp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Stimulates adipogenesis (By similarity).By similarity

Miscellaneous

The limitation of proteolytic products to tripeptides is achieved by tailoring the size of the substrate-binding cleft: the two negatively charged residues Glu-305 and Glu-331 that are blocking position P4 limit the number of residues that can be accommodated in the binding cleft and thus create a molecular ruler. At the same time, they orient substrates so that the tripeptides are removed exclusively from the N-terminus (By similarity).By similarity

Catalytic activityi

Release of an N-terminal tripeptide from a polypeptide.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Charge relay systemBy similarity1
Active sitei264Charge relay systemBy similarity1
Active sitei449Charge relay systemBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB-KW
  • peptide binding Source: RGD
  • serine-type endopeptidase activity Source: InterPro
  • tripeptidyl-peptidase activity Source: RGD

GO - Biological processi

  • proteolysis Source: RGD

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiS08.A56

Names & Taxonomyi

Protein namesi
Recommended name:
Tripeptidyl-peptidase 2 (EC:3.4.14.10)
Short name:
TPP-2
Alternative name(s):
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase II
Short name:
TPP-II
Gene namesi
Name:Tpp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi621584 Tpp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4675

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000764242 – 1249Tripeptidyl-peptidase 2Add BLAST1248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei401N6-acetyllysineBy similarity1
Modified residuei915PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ64560
PRIDEiQ64560

PTM databases

iPTMnetiQ64560
PhosphoSitePlusiQ64560

Expressioni

Gene expression databases

BgeeiENSRNOG00000011194
GenevisibleiQ64560 RN

Interactioni

Protein-protein interaction databases

BioGridi249673, 1 interactor
IntActiQ64560, 1 interactor
STRINGi10116.ENSRNOP00000015393

Chemistry databases

BindingDBiQ64560

Structurei

3D structure databases

ProteinModelPortaliQ64560
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 509Peptidase S8Add BLAST470

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Phylogenomic databases

eggNOGiKOG1114 Eukaryota
COG1404 LUCA
GeneTreeiENSGT00390000014623
HOGENOMiHOG000008178
HOVERGENiHBG017992
InParanoidiQ64560
KOiK01280
OMAiWRACIDT
OrthoDBiEOG091G00P0

Family and domain databases

CDDicd04857 Peptidases_S8_Tripeptidyl_Amin, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR022229 Peptidase_S8A_TPPII
IPR034051 TPP_II_domain
PfamiView protein in Pfam
PF00082 Peptidase_S8, 1 hit
PF12580 TPPII, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 2 hits
PROSITEiView protein in PROSITE
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAATEEPF PFHGLLPKKE TGASSFLCRY PEYDGRGVLI AVLDTGVDPG
60 70 80 90 100
APGMQVTTDG KPKIIDIIDT TGSGDVNTAT EVEPKDGEIT GLSGRVLKIP
110 120 130 140 150
ANWTNPSGKY HIGIKNGYDF YPKALKERIQ KERKEKIWDP IHRVALAEAC
160 170 180 190 200
RKQEEFDIAN NGSSQANKLI KEELQSQVEL LNSFEKKYSD PGPVYDCLVW
210 220 230 240 250
HDGETWRACV DSNENGDLGK STVLRNYKEA QEYGSFGTAE MLNYSVNIYD
260 270 280 290 300
DGNLLSIVTS GGAHGTHVAS IAAGHFPEEP ERNGVAPGAQ ILSIKIGDTR
310 320 330 340 350
LSTMETGTGL IRAMIEVINH KCDLVNYSYG EATHWPNSGR ICEVINEAVW
360 370 380 390 400
KHNTIYVSSA GNNGPCLSTV GCPGGTTSSV IGVGAYVSPD MMVAEYSLRE
410 420 430 440 450
KLPANQYTWS SRGPSADGAL GVSISAPGGA IASVPNWTLR GTQLMNGTSM
460 470 480 490 500
SSPNACGGIA LVLSGLKANN VDYTVHSVRR ALENTAIKAD NIEVFAQGHG
510 520 530 540 550
IIQVDKAYDY LIQNTSFANR LGFTVTVGNN RGIYLRDPVQ VAAPSDHGVG
560 570 580 590 600
IEPVFPENTE NSEKISFQLH LALTSNSSWV QCPSHLELMN QCRHINIRVD
610 620 630 640 650
PRGLREGLHY TEVCGYDIAS PNAGPLFRVP ITAVIAAKVN ESSHYDLAFT
660 670 680 690 700
DVHFKPGQIR RHFVEVPEGA TWAEVTVCSC SSEVSAKFVL HAVQLVKQRA
710 720 730 740 750
YRSHEFYKFC SLPEKGTLIE AFPVLGGKAI EFCIARWWAS LSDVNIDYTI
760 770 780 790 800
SFHGIVCTAP QLNIHASEGI NRFDVQSSLK YEDLAPCITL KSWVQTLRPV
810 820 830 840 850
NAKTRPLGSR DVLPNNRQLY EMVLTYSFHQ PKSGEVTPSC PLLCELLYES
860 870 880 890 900
EFDSQLWIIF DQNKRQMGSG DAYPHQYSLK LEKGDYTIRL QIRHEQISDL
910 920 930 940 950
DRLKDLPFIV SHRLSNTLSL DIHENHSLAL LGKKKSSSLT LPPKYNQPFF
960 970 980 990 1000
VTSLPDDKIP KGAGPGCYLA GSLTLSKTEL GKKADVIPVH YYLIPPPTKT
1010 1020 1030 1040 1050
KNGSKDKEKD SEKEKDLKEE FTEALRDLKI QWMTKLDSTD IYNELKETYP
1060 1070 1080 1090 1100
AYLPLYVARL HQLDAEKERM KRLNEIVDAA NAVISHIDQT ALAVYIAMKT
1110 1120 1130 1140 1150
DPRPDAATIK NDMDKQKSTL VDALCRKGCA LADHLLHAQP HDGAAAGDAE
1160 1170 1180 1190 1200
AKEEEGESTL ESLSETYWET TKWTDLFDTK VLTFAYKHAL VNKMYGRGLK
1210 1220 1230 1240
FATKLVEEKP TKENWKNCIQ LMKLLGWTHC ASFTENWLPI MYPPDYCVF
Length:1,249
Mass (Da):138,293
Last modified:January 23, 2007 - v3
Checksum:iF4A41664028AAB2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50194 mRNA Translation: AAA93458.1
PIRiS68431
RefSeqiNP_112399.1, NM_031137.1
UniGeneiRn.28991

Genome annotation databases

EnsembliENSRNOT00000015393; ENSRNOP00000015393; ENSRNOG00000011194
ENSRNOT00000078220; ENSRNOP00000074045; ENSRNOG00000011194
ENSRNOT00000085267; ENSRNOP00000074556; ENSRNOG00000011194
GeneIDi81815
KEGGirno:81815
UCSCiRGD:621584 rat

Similar proteinsi

Entry informationi

Entry nameiTPP2_RAT
AccessioniPrimary (citable) accession number: Q64560
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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