Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q64550 (UD11_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glucuronosyltransferase 1-1

Short name=UDPGT 1-1
Short name=UGT1*1
Short name=UGT1-01
Short name=UGT1.1
EC=2.4.1.17
Alternative name(s):
B1
UDP-glucuronosyltransferase 1A1
Gene names
Name:Ugt1a1
Synonyms:Ugt1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length535 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Glucuronates opioids and bilirubin.

Catalytic activity

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

Subunit structure

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

Subcellular location

Microsome. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.

Induction

Overexpressed of two- to threefold upon clofibrate and dexamethasone administration. Ref.4

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processacute-phase response

Inferred from expression pattern PubMed 9737578. Source: RGD

bilirubin conjugation

Traceable author statement PubMed 11829457. Source: RGD

biphenyl catabolic process

Inferred from direct assay PubMed 16006569. Source: RGD

cellular response to ethanol

Inferred from expression pattern PubMed 11412396. Source: RGD

cellular response to glucocorticoid stimulus

Inferred from expression pattern PubMed 10100302. Source: RGD

cellular response to hormone stimulus

Inferred from expression pattern PubMed 10100302. Source: RGD

flavone metabolic process

Inferred from electronic annotation. Source: Ensembl

flavonoid glucuronidation

Inferred from electronic annotation. Source: Ensembl

liver development

Inferred from expression pattern PubMed 16310220PubMed 18938141. Source: RGD

organ regeneration

Inferred from expression pattern PubMed 16222444. Source: RGD

response to drug

Inferred from expression pattern PubMed 11829457. Source: RGD

response to ethanol

Inferred from expression pattern PubMed 11848303. Source: RGD

response to glucocorticoid

Inferred from expression pattern PubMed 11829457. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern PubMed 19356101. Source: RGD

response to nutrient

Inferred from expression pattern PubMed 11299074. Source: RGD

response to organic cyclic compound

Inferred from expression pattern PubMed 11854140. Source: RGD

response to organic substance

Inferred from mutant phenotype PubMed 15921999. Source: RGD

response to starvation

Inferred from expression pattern PubMed 9841869. Source: RGD

response to steroid hormone

Inferred from expression pattern PubMed 11086234. Source: RGD

xenobiotic glucuronidation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytochrome complex

Inferred from direct assay PubMed 17965520. Source: RGD

endoplasmic reticulum

Inferred from direct assay PubMed 14561759. Source: HGNC

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 9841869. Source: RGD

   Molecular_functionenzyme binding

Inferred from direct assay PubMed 17965520. Source: RGD

enzyme inhibitor activity

Inferred from electronic annotation. Source: Ensembl

glucuronosyltransferase activity

Inferred from direct assay PubMed 11829457. Source: RGD

retinoic acid binding

Inferred from electronic annotation. Source: Ensembl

steroid binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. The different isozymes have a different N-terminal domain and a common C-terminal domain of 245 residues.
Isoform 1 (identifier: Q64550-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Ref.3
Chain30 – 535506UDP-glucuronosyltransferase 1-1
PRO_0000036011

Regions

Transmembrane493 – 50917Helical; Potential

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation4351N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2531A → D in BAA07260. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 26B642FCA7DD4082

FASTA53559,663
        10         20         30         40         50         60 
MSVVCRSSCS LLLLPCLLLC VLGPSASHAG KLLVIPIDGS HWLSMLGVIQ QLQQKGHEVV 

        70         80         90        100        110        120 
VIAPEASIHI KEGSFYTMRK YPVPFQNENV TAAFVELGRS VFDQDPFLLR VVKTYNKVKR 

       130        140        150        160        170        180 
DSSMLLSGCS HLLHNAEFMA SLEQSHFDAL LTDPFLPCGS IVAQYLSLPA VYFLNALPCS 

       190        200        210        220        230        240 
LDLEATQCPA PLSYVPKSLS SNTDRMNFLQ RVKNMIIALT ENFLCRVVYS PYGSLATEIL 

       250        260        270        280        290        300 
QKEVTVKDLL SPASIWLMRN DFVKDYPRPI MPNMVFIGGI NCLQKKALSQ EFEAYVNASG 

       310        320        330        340        350        360 
EHGIVVFSLG SMVSEIPEKK AMEIAEALGR IPQTVLWRYT GTRPSNLAKN TILVKWLPQN 

       370        380        390        400        410        420 
DLLGHPKARA FITHSGSHGI YEGICNGVPM VMMPLFGDQM DNAKRMETRG AGVTLNVLEM 

       430        440        450        460        470        480 
TADDLENALK TVINNKSYKE NIMRLSSLHK DRPIEPLDLA VFWVEYVMRH KGAPHLRPAA 

       490        500        510        520        530 
HDLTWYQYHS LDVIGFLLAI VLTVVFIVYK SCAYGCRKCF GGKGRVKKSH KSKTH 

« Hide

References

[1]"Cloning and stable expression of a cDNA encoding a rat liver UDP-glucuronosyltransferase (UDP-glucuronosyltransferase 1.1) that catalyzes the glucuronidation of opioids and bilirubin."
Coffman B.L., Green M.D., King C.O., Tephly T.R.
Mol. Pharmacol. 47:1101-1105(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Wistar.
[2]"Drug-responsive and tissue-specific alternative expression of multiple first exons in rat UDP-glucuronosyltransferase family 1 (UGT1) gene complex."
Emi Y., Ikushiro S., Iyanagi T.
J. Biochem. 117:392-399(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-290.
Strain: Wistar.
[3]"Purification of a phenobarbital-inducible morphine UDP-glucuronyltransferase isoform, absent from Gunn rat liver."
Ishii Y., Tsuruda K., Tanaka M., Oguri K.
Arch. Biochem. Biophys. 315:345-351(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 30-41.
[4]"Identification and analysis of drug-responsive expression of UDP-glucuronosyltransferase family 1 (UGT1) isozyme in rat hepatic microsomes using anti-peptide antibodies."
Ikushiro S., Emi Y., Iyanagi T.
Arch. Biochem. Biophys. 324:267-272(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[5]"A subset of chaperones and folding enzymes form multiprotein complexes in endoplasmic reticulum to bind nascent proteins."
Meunier L., Usherwood Y.-K., Chung K.T., Hendershot L.M.
Mol. Biol. Cell 13:4456-4469(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPONENT OF A CHAPERONE COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20551 Genomic DNA. Translation: AAC52219.1.
D38065 Genomic DNA. Translation: BAA07260.1.
PIRI57961.
RefSeqNP_036815.1. NM_012683.2. [Q64550-1]
UniGeneRn.26489.

3D structure databases

ProteinModelPortalQ64550.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbQ64550.
PRIDEQ64550.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025045; ENSRNOP00000025045; ENSRNOG00000018740. [Q64550-1]
GeneID24861.
KEGGrno:24861.

Organism-specific databases

CTD54658.
RGD3935. Ugt1a1.

Phylogenomic databases

eggNOGCOG1819.
GeneTreeENSGT00640000091365.
HOGENOMHOG000220832.
HOVERGENHBG004033.
InParanoidQ64550.
KOK00699.
OMASEIDEAY.
TreeFamTF315472.

Enzyme and pathway databases

BRENDA2.4.1.17. 5301.

Gene expression databases

ArrayExpressQ64550.
GenevestigatorQ64550.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604668.

Entry information

Entry nameUD11_RAT
AccessionPrimary (citable) accession number: Q64550
Secondary accession number(s): Q64635
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families