Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-glucuronosyltransferase 1-1

Gene

Ugt1a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Glucuronates opioids and bilirubin.

Catalytic activityi

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

GO - Molecular functioni

  1. enzyme binding Source: RGD
  2. enzyme inhibitor activity Source: Ensembl
  3. glucuronosyltransferase activity Source: RGD
  4. retinoic acid binding Source: Ensembl
  5. steroid binding Source: Ensembl

GO - Biological processi

  1. acute-phase response Source: RGD
  2. bilirubin conjugation Source: RGD
  3. biphenyl catabolic process Source: RGD
  4. cellular response to ethanol Source: RGD
  5. cellular response to glucocorticoid stimulus Source: RGD
  6. cellular response to hormone stimulus Source: RGD
  7. flavone metabolic process Source: Ensembl
  8. flavonoid biosynthetic process Source: GO_Central
  9. flavonoid glucuronidation Source: GO_Central
  10. liver development Source: RGD
  11. negative regulation of cellular glucuronidation Source: Ensembl
  12. organ regeneration Source: RGD
  13. response to drug Source: RGD
  14. response to ethanol Source: RGD
  15. response to glucocorticoid Source: RGD
  16. response to lipopolysaccharide Source: RGD
  17. response to nutrient Source: RGD
  18. response to organic cyclic compound Source: RGD
  19. response to organic substance Source: RGD
  20. response to starvation Source: RGD
  21. response to steroid hormone Source: RGD
  22. xenobiotic glucuronidation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.17. 5301.
ReactomeiREACT_221439. Glucuronidation.
REACT_251296. Heme degradation.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronosyltransferase 1-1 (EC:2.4.1.17)
Short name:
UDPGT 1-1
Short name:
UGT1*1
Short name:
UGT1-01
Short name:
UGT1.1
Alternative name(s):
B1
UDP-glucuronosyltransferase 1A1
Gene namesi
Name:Ugt1a1
Synonyms:Ugt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi3935. Ugt1a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei493 – 50917HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytochrome complex Source: RGD
  2. endoplasmic reticulum Source: HGNC
  3. endoplasmic reticulum membrane Source: GO_Central
  4. integral component of plasma membrane Source: Ensembl
  5. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 535506UDP-glucuronosyltransferase 1-1PRO_0000036011Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi435 – 4351N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ64550.
PRIDEiQ64550.

Expressioni

Inductioni

Overexpressed of two- to threefold upon clofibrate and dexamethasone administration.1 Publication

Gene expression databases

ExpressionAtlasiQ64550. baseline and differential.
GenevestigatoriQ64550.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

Structurei

3D structure databases

ProteinModelPortaliQ64550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220832.
HOVERGENiHBG004033.
InParanoidiQ64550.
KOiK00699.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. The different isozymes have a different N-terminal domain and a common C-terminal domain of 245 residues.

Isoform 1 (identifier: Q64550-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVVCRSSCS LLLLPCLLLC VLGPSASHAG KLLVIPIDGS HWLSMLGVIQ
60 70 80 90 100
QLQQKGHEVV VIAPEASIHI KEGSFYTMRK YPVPFQNENV TAAFVELGRS
110 120 130 140 150
VFDQDPFLLR VVKTYNKVKR DSSMLLSGCS HLLHNAEFMA SLEQSHFDAL
160 170 180 190 200
LTDPFLPCGS IVAQYLSLPA VYFLNALPCS LDLEATQCPA PLSYVPKSLS
210 220 230 240 250
SNTDRMNFLQ RVKNMIIALT ENFLCRVVYS PYGSLATEIL QKEVTVKDLL
260 270 280 290 300
SPASIWLMRN DFVKDYPRPI MPNMVFIGGI NCLQKKALSQ EFEAYVNASG
310 320 330 340 350
EHGIVVFSLG SMVSEIPEKK AMEIAEALGR IPQTVLWRYT GTRPSNLAKN
360 370 380 390 400
TILVKWLPQN DLLGHPKARA FITHSGSHGI YEGICNGVPM VMMPLFGDQM
410 420 430 440 450
DNAKRMETRG AGVTLNVLEM TADDLENALK TVINNKSYKE NIMRLSSLHK
460 470 480 490 500
DRPIEPLDLA VFWVEYVMRH KGAPHLRPAA HDLTWYQYHS LDVIGFLLAI
510 520 530
VLTVVFIVYK SCAYGCRKCF GGKGRVKKSH KSKTH
Length:535
Mass (Da):59,663
Last modified:November 1, 1997 - v1
Checksum:i26B642FCA7DD4082
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2531A → D in BAA07260. (PubMed:7608130)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20551 Genomic DNA. Translation: AAC52219.1.
D38065 Genomic DNA. Translation: BAA07260.1.
PIRiI57961.
RefSeqiNP_036815.1. NM_012683.2. [Q64550-1]
UniGeneiRn.26489.

Genome annotation databases

EnsembliENSRNOT00000025045; ENSRNOP00000025045; ENSRNOG00000018740. [Q64550-1]
GeneIDi24861.
KEGGirno:24861.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20551 Genomic DNA. Translation: AAC52219.1.
D38065 Genomic DNA. Translation: BAA07260.1.
PIRiI57961.
RefSeqiNP_036815.1. NM_012683.2. [Q64550-1]
UniGeneiRn.26489.

3D structure databases

ProteinModelPortaliQ64550.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbiQ64550.
PRIDEiQ64550.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025045; ENSRNOP00000025045; ENSRNOG00000018740. [Q64550-1]
GeneIDi24861.
KEGGirno:24861.

Organism-specific databases

CTDi54658.
RGDi3935. Ugt1a1.

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220832.
HOVERGENiHBG004033.
InParanoidiQ64550.
KOiK00699.
TreeFamiTF315472.

Enzyme and pathway databases

BRENDAi2.4.1.17. 5301.
ReactomeiREACT_221439. Glucuronidation.
REACT_251296. Heme degradation.

Miscellaneous databases

NextBioi604668.

Gene expression databases

ExpressionAtlasiQ64550. baseline and differential.
GenevestigatoriQ64550.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and stable expression of a cDNA encoding a rat liver UDP-glucuronosyltransferase (UDP-glucuronosyltransferase 1.1) that catalyzes the glucuronidation of opioids and bilirubin."
    Coffman B.L., Green M.D., King C.O., Tephly T.R.
    Mol. Pharmacol. 47:1101-1105(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wistar.
  2. "Drug-responsive and tissue-specific alternative expression of multiple first exons in rat UDP-glucuronosyltransferase family 1 (UGT1) gene complex."
    Emi Y., Ikushiro S., Iyanagi T.
    J. Biochem. 117:392-399(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-290.
    Strain: Wistar.
  3. "Purification of a phenobarbital-inducible morphine UDP-glucuronyltransferase isoform, absent from Gunn rat liver."
    Ishii Y., Tsuruda K., Tanaka M., Oguri K.
    Arch. Biochem. Biophys. 315:345-351(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-41.
  4. "Identification and analysis of drug-responsive expression of UDP-glucuronosyltransferase family 1 (UGT1) isozyme in rat hepatic microsomes using anti-peptide antibodies."
    Ikushiro S., Emi Y., Iyanagi T.
    Arch. Biochem. Biophys. 324:267-272(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "A subset of chaperones and folding enzymes form multiprotein complexes in endoplasmic reticulum to bind nascent proteins."
    Meunier L., Usherwood Y.-K., Chung K.T., Hendershot L.M.
    Mol. Biol. Cell 13:4456-4469(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF A CHAPERONE COMPLEX.

Entry informationi

Entry nameiUD11_RAT
AccessioniPrimary (citable) accession number: Q64550
Secondary accession number(s): Q64635
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 4, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.