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Q64542

- AT2B4_RAT

UniProt

Q64542 - AT2B4_RAT

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Protein
Plasma membrane calcium-transporting ATPase 4
Gene
Atp2b4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei465 – 46514-aspartylphosphate intermediate By similarity
Metal bindingi785 – 7851Magnesium By similarity
Metal bindingi789 – 7891Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. PDZ domain binding Source: RGD
  3. calcium-transporting ATPase activity Source: RGD
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. calcium ion export Source: RGD
  2. calcium ion transmembrane transport Source: RGD
  3. calcium ion transport Source: RGD
  4. cellular calcium ion homeostasis Source: RGD
  5. hippocampus development Source: RGD
  6. neural retina development Source: RGD
  7. spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 4 (EC:3.6.3.8)
Short name:
PMCA4
Alternative name(s):
Plasma membrane calcium ATPase isoform 4
Plasma membrane calcium pump isoform 4
Gene namesi
Name:Atp2b4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621305. Atp2b4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9292Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei93 – 11321Helical; Reviewed prediction
Add
BLAST
Topological domaini114 – 15037Extracellular Reviewed prediction
Add
BLAST
Transmembranei151 – 17121Helical; Reviewed prediction
Add
BLAST
Topological domaini172 – 356185Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei357 – 37620Helical; Reviewed prediction
Add
BLAST
Topological domaini377 – 40933Extracellular Reviewed prediction
Add
BLAST
Transmembranei410 – 42718Helical; Reviewed prediction
Add
BLAST
Topological domaini428 – 840413Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei841 – 86020Helical; Reviewed prediction
Add
BLAST
Topological domaini861 – 87010Extracellular Reviewed prediction
Transmembranei871 – 89121Helical; Reviewed prediction
Add
BLAST
Topological domaini892 – 91120Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei912 – 93423Helical; Reviewed prediction
Add
BLAST
Topological domaini935 – 95218Extracellular Reviewed prediction
Add
BLAST
Transmembranei953 – 97422Helical; Reviewed prediction
Add
BLAST
Topological domaini975 – 99319Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei994 – 101522Helical; Reviewed prediction
Add
BLAST
Topological domaini1016 – 102510Extracellular Reviewed prediction
Transmembranei1026 – 104722Helical; Reviewed prediction
Add
BLAST
Topological domaini1048 – 1203156Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. neuron projection Source: RGD
  4. sperm principal piece Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12031203Plasma membrane calcium-transporting ATPase 4
PRO_0000046221Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1102 – 11021Phosphothreonine; by PKC By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64542.
PRIDEiQ64542.

PTM databases

PhosphoSiteiQ64542.

Expressioni

Tissue specificityi

Ubiquitously expressed. Not detected in liver. The highest levels are found in uterus and stomach. Isoform XA is found in uterus, brain, stomach, small intestine, colon and pancreas. Isoform XB is found in uterus, skeletal muscle, lung, kidney, spleen, stomach, small intestine and pancreas. Isoform ZA is found in testis and isoform ZB is found in testis and heart.

Gene expression databases

GenevestigatoriQ64542.

Interactioni

Subunit structurei

Interacts with PDZD11 By similarity.

Protein-protein interaction databases

BioGridi248232. 1 interaction.
MINTiMINT-4997403.

Structurei

3D structure databases

ProteinModelPortaliQ64542.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1086 – 110318Calmodulin-binding subdomain A By similarity
Add
BLAST
Regioni1104 – 111310Calmodulin-binding subdomain B By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi297 – 3037Poly-Lys

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ64542.
KOiK05850.
PhylomeDBiQ64542.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: There is a combination of two alternatively spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A and B).

Isoform XB (identifier: Q64542-1) [UniParc]FASTAAdd to Basket

Also known as: AIICI

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTNPSGHNLP ANSVAESYEG EFGCTLMDLR KLMELRAADA VTQISAHYGS     50
VQEICARLKT SPVEGLSGNP ADLEKRRLVF GKNMIPPKKP KTFLELVWEA 100
LQDVTLIILE IAAIISLVLS FYRPPGGENE ICGHIVSNPE EDEEGETGWI 150
EGAAILASVI IVVFVTAFND WSKEKQFRGL QSRIELEQKF SIIRNGQLIQ 200
LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT GESDHVKKTL 250
DKDPMLLSGT HVMEGSGRMV VTAVGINSQT GIIFTLLGAN EEEDDEKKKK 300
GKKQGVSENR NKAKTQDGVA LEIQPLNSQE GLDSEEKEKK ASKGPKKEKS 350
VLQGKLTRLA VQIGKAGLIM SILTVLILIL YFVVDNFVIQ RRAWLPECTP 400
VYIQYFVKFF IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL 450
DACETMGNAT AICSDKTGTL TMNRMTVVQA YIGGTHYRQI PKPDDLPPNV 500
LDLIVNSICI NSAYTSKILP PEKEGGLPRQ VGNKTECGLL GFVTDLKQDY 550
QAVRSEMPEE KLFKVYTFNS VRKSMSTVIR KPEGGFRVFS KGASEIMLRK 600
CDRILNKEGG IVPFKTKDRD NMVRNVIEPM ASEGLRTIGI AYRDFDGEEP 650
SWENENEIFT GLVCIAVVGI EDPVRPEVPD AINKCKRAGI TVRMVTGDNV 700
NTARAIATKC GILTPGDDFL CLEGKEFNRL IRNEKGEVEQ EKLDKVWPRL 750
RVLARSSPTD KHTLVKGIID SNIGEQRQVV AVTGDGTNDG PALKKADVGF 800
AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI SKFLQFQLTV 850
NVVAVIVAFS GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTDSLL 900
RRRPYGRNKP LISRTMMKNI LGHAVYQLGI VFLLVFAGDK LFDIDSGRKA 950
PLNSPPSQHY TIVFNTFVLM QLFNEINSRK IHGEKNVFAG VYRNIIFCSV 1000
VLGTFFCQIL IVEVGGKPFS CTNLTMEQWM WCLFIGIGEL LWGQVISAIP 1050
TKSLKFLKEA GHGSDKEEIS KDAEGLEEID HAEMELRRGQ ILWVRGLNRI 1100
QTQIRVVKVF HSFRDVIHKS KNQVSIHSFM TQPEYAADDE MSQSFLNQEE 1150
SPSLASKSRI TKRLSDAETV SQNNTNNNAV DCHQVQIVAS HPNSPLQSQE 1200
TPV 1203
Length:1,203
Mass (Da):133,094
Last modified:November 1, 1996 - v1
Checksum:iB7A6C8D4556F6398
GO
Isoform XA (identifier: Q64542-2) [UniParc]FASTAAdd to Basket

Also known as: AIICII

The sequence of this isoform differs from the canonical sequence as follows:
     1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS

Show »
Length:1,169
Mass (Da):128,791
Checksum:iC799FA6AC708EBE4
GO
Isoform ZA (identifier: Q64542-3) [UniParc]FASTAAdd to Basket

Also known as: AICII

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.
     1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS

Show »
Length:1,157
Mass (Da):127,464
Checksum:iEDB410076D59B247
GO
Isoform ZB (identifier: Q64542-4) [UniParc]FASTAAdd to Basket

Also known as: AICI

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.

Show »
Length:1,191
Mass (Da):131,768
Checksum:iD93D8B4839575FDB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei301 – 31212Missing in isoform ZA and isoform ZB.
VSP_000406Add
BLAST
Alternative sequencei1105 – 120399RVVKV…QETPV → EVINKFQTGASFKGVLRRQN LSQQLDVKLVPSSYSEAVAS VRTSPSTSSAVTPPPVGNQS GQSIS in isoform XA and isoform ZA.
VSP_000407Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti431 – 4311I → V in CAA53990. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15408 mRNA. Translation: AAA81005.1.
U15408 mRNA. Translation: AAA81006.1.
U15408 mRNA. Translation: AAA81007.1.
U15408 mRNA. Translation: AAA81008.1.
X76452 mRNA. Translation: CAA53990.1.
L05569, L05566, L05567 Genomic DNA. Translation: AAA50820.1.
PIRiG44525.
S50027.
S54356.
S54357.
RefSeqiNP_001005871.1. NM_001005871.1. [Q64542-2]
UniGeneiRn.9986.

Genome annotation databases

GeneIDi29600.
KEGGirno:29600.
UCSCiRGD:621305. rat. [Q64542-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15408 mRNA. Translation: AAA81005.1 .
U15408 mRNA. Translation: AAA81006.1 .
U15408 mRNA. Translation: AAA81007.1 .
U15408 mRNA. Translation: AAA81008.1 .
X76452 mRNA. Translation: CAA53990.1 .
L05569 , L05566 , L05567 Genomic DNA. Translation: AAA50820.1 .
PIRi G44525.
S50027.
S54356.
S54357.
RefSeqi NP_001005871.1. NM_001005871.1. [Q64542-2 ]
UniGenei Rn.9986.

3D structure databases

ProteinModelPortali Q64542.
ModBasei Search...

Protein-protein interaction databases

BioGridi 248232. 1 interaction.
MINTi MINT-4997403.

PTM databases

PhosphoSitei Q64542.

Proteomic databases

PaxDbi Q64542.
PRIDEi Q64542.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29600.
KEGGi rno:29600.
UCSCi RGD:621305. rat. [Q64542-1 ]

Organism-specific databases

CTDi 493.
RGDi 621305. Atp2b4.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265623.
HOVERGENi HBG061286.
InParanoidi Q64542.
KOi K05850.
PhylomeDBi Q64542.

Miscellaneous databases

NextBioi 609760.
PROi Q64542.

Gene expression databases

Genevestigatori Q64542.

Family and domain databases

Gene3Di 1.20.1110.10. 3 hits.
InterProi IPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of rat plasma membrane Ca(2+)-ATPase isoform 4 and analysis of alternative splicing patterns at splice site A."
    Keeton T.P., Shull G.E.
    Biochem. J. 306:779-785(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS XB; ZB; XA AND ZA).
    Strain: CD Charles River.
    Tissue: Testis.
  2. "Plasma-membrane calcium-pump isoforms in human and rat liver."
    Howard A., Barley N.F., Legon S., Walters J.R.F.
    Biochem. J. 303:275-279(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 266-455 (ISOFORMS XA/XB).
    Tissue: Lung.
  3. "Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
    Keeton T.P., Burk S.E., Shull G.E.
    J. Biol. Chem. 268:2740-2748(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1081-1203, ALTERNATIVE SPLICING (ISOFORMS XA/ZA).
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiAT2B4_RAT
AccessioniPrimary (citable) accession number: Q64542
Secondary accession number(s): Q63127
, Q63445, Q64543, Q64544, Q64545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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