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Q64542

- AT2B4_RAT

UniProt

Q64542 - AT2B4_RAT

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Protein

Plasma membrane calcium-transporting ATPase 4

Gene

Atp2b4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei465 – 46514-aspartylphosphate intermediateBy similarity
Metal bindingi785 – 7851MagnesiumBy similarity
Metal bindingi789 – 7891MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium-transporting ATPase activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW
  4. PDZ domain binding Source: RGD

GO - Biological processi

  1. calcium ion export Source: RGD
  2. calcium ion transmembrane transport Source: RGD
  3. calcium ion transport Source: RGD
  4. cellular calcium ion homeostasis Source: RGD
  5. hippocampus development Source: RGD
  6. negative regulation of calcineurin-NFAT signaling cascade Source: BHF-UCL
  7. neural retina development Source: RGD
  8. spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 4 (EC:3.6.3.8)
Short name:
PMCA4
Alternative name(s):
Plasma membrane calcium ATPase isoform 4
Plasma membrane calcium pump isoform 4
Gene namesi
Name:Atp2b4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621305. Atp2b4.

Subcellular locationi

GO - Cellular componenti

  1. basolateral plasma membrane Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. neuron projection Source: RGD
  4. sperm principal piece Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12031203Plasma membrane calcium-transporting ATPase 4PRO_0000046221Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1102 – 11021Phosphothreonine; by PKCBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64542.
PRIDEiQ64542.

PTM databases

PhosphoSiteiQ64542.

Expressioni

Tissue specificityi

Ubiquitously expressed. Not detected in liver. The highest levels are found in uterus and stomach. Isoform XA is found in uterus, brain, stomach, small intestine, colon and pancreas. Isoform XB is found in uterus, skeletal muscle, lung, kidney, spleen, stomach, small intestine and pancreas. Isoform ZA is found in testis and isoform ZB is found in testis and heart.

Gene expression databases

GenevestigatoriQ64542.

Interactioni

Subunit structurei

Interacts with PDZD11. Interacts with SLC35G1 and STIM1.By similarity

Protein-protein interaction databases

BioGridi248232. 1 interaction.
MINTiMINT-4997403.

Structurei

3D structure databases

ProteinModelPortaliQ64542.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9292CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini114 – 15037ExtracellularSequence AnalysisAdd
BLAST
Topological domaini172 – 356185CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini377 – 40933ExtracellularSequence AnalysisAdd
BLAST
Topological domaini428 – 840413CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini861 – 87010ExtracellularSequence Analysis
Topological domaini892 – 91120CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini935 – 95218ExtracellularSequence AnalysisAdd
BLAST
Topological domaini975 – 99319CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini1016 – 102510ExtracellularSequence Analysis
Topological domaini1048 – 1203156CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei93 – 11321HelicalSequence AnalysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence AnalysisAdd
BLAST
Transmembranei357 – 37620HelicalSequence AnalysisAdd
BLAST
Transmembranei410 – 42718HelicalSequence AnalysisAdd
BLAST
Transmembranei841 – 86020HelicalSequence AnalysisAdd
BLAST
Transmembranei871 – 89121HelicalSequence AnalysisAdd
BLAST
Transmembranei912 – 93423HelicalSequence AnalysisAdd
BLAST
Transmembranei953 – 97422HelicalSequence AnalysisAdd
BLAST
Transmembranei994 – 101522HelicalSequence AnalysisAdd
BLAST
Transmembranei1026 – 104722HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1086 – 110318Calmodulin-binding subdomain ABy similarityAdd
BLAST
Regioni1104 – 111310Calmodulin-binding subdomain BBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi297 – 3037Poly-Lys

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ64542.
KOiK05850.
PhylomeDBiQ64542.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: There is a combination of two alternatively spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A and B).

Isoform XB (identifier: Q64542-1) [UniParc]FASTAAdd to Basket

Also known as: AIICI

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNPSGHNLP ANSVAESYEG EFGCTLMDLR KLMELRAADA VTQISAHYGS
60 70 80 90 100
VQEICARLKT SPVEGLSGNP ADLEKRRLVF GKNMIPPKKP KTFLELVWEA
110 120 130 140 150
LQDVTLIILE IAAIISLVLS FYRPPGGENE ICGHIVSNPE EDEEGETGWI
160 170 180 190 200
EGAAILASVI IVVFVTAFND WSKEKQFRGL QSRIELEQKF SIIRNGQLIQ
210 220 230 240 250
LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT GESDHVKKTL
260 270 280 290 300
DKDPMLLSGT HVMEGSGRMV VTAVGINSQT GIIFTLLGAN EEEDDEKKKK
310 320 330 340 350
GKKQGVSENR NKAKTQDGVA LEIQPLNSQE GLDSEEKEKK ASKGPKKEKS
360 370 380 390 400
VLQGKLTRLA VQIGKAGLIM SILTVLILIL YFVVDNFVIQ RRAWLPECTP
410 420 430 440 450
VYIQYFVKFF IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL
460 470 480 490 500
DACETMGNAT AICSDKTGTL TMNRMTVVQA YIGGTHYRQI PKPDDLPPNV
510 520 530 540 550
LDLIVNSICI NSAYTSKILP PEKEGGLPRQ VGNKTECGLL GFVTDLKQDY
560 570 580 590 600
QAVRSEMPEE KLFKVYTFNS VRKSMSTVIR KPEGGFRVFS KGASEIMLRK
610 620 630 640 650
CDRILNKEGG IVPFKTKDRD NMVRNVIEPM ASEGLRTIGI AYRDFDGEEP
660 670 680 690 700
SWENENEIFT GLVCIAVVGI EDPVRPEVPD AINKCKRAGI TVRMVTGDNV
710 720 730 740 750
NTARAIATKC GILTPGDDFL CLEGKEFNRL IRNEKGEVEQ EKLDKVWPRL
760 770 780 790 800
RVLARSSPTD KHTLVKGIID SNIGEQRQVV AVTGDGTNDG PALKKADVGF
810 820 830 840 850
AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI SKFLQFQLTV
860 870 880 890 900
NVVAVIVAFS GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTDSLL
910 920 930 940 950
RRRPYGRNKP LISRTMMKNI LGHAVYQLGI VFLLVFAGDK LFDIDSGRKA
960 970 980 990 1000
PLNSPPSQHY TIVFNTFVLM QLFNEINSRK IHGEKNVFAG VYRNIIFCSV
1010 1020 1030 1040 1050
VLGTFFCQIL IVEVGGKPFS CTNLTMEQWM WCLFIGIGEL LWGQVISAIP
1060 1070 1080 1090 1100
TKSLKFLKEA GHGSDKEEIS KDAEGLEEID HAEMELRRGQ ILWVRGLNRI
1110 1120 1130 1140 1150
QTQIRVVKVF HSFRDVIHKS KNQVSIHSFM TQPEYAADDE MSQSFLNQEE
1160 1170 1180 1190 1200
SPSLASKSRI TKRLSDAETV SQNNTNNNAV DCHQVQIVAS HPNSPLQSQE

TPV
Length:1,203
Mass (Da):133,094
Last modified:November 1, 1996 - v1
Checksum:iB7A6C8D4556F6398
GO
Isoform XA (identifier: Q64542-2) [UniParc]FASTAAdd to Basket

Also known as: AIICII

The sequence of this isoform differs from the canonical sequence as follows:
     1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS

Show »
Length:1,169
Mass (Da):128,791
Checksum:iC799FA6AC708EBE4
GO
Isoform ZA (identifier: Q64542-3) [UniParc]FASTAAdd to Basket

Also known as: AICII

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.
     1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS

Show »
Length:1,157
Mass (Da):127,464
Checksum:iEDB410076D59B247
GO
Isoform ZB (identifier: Q64542-4) [UniParc]FASTAAdd to Basket

Also known as: AICI

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.

Show »
Length:1,191
Mass (Da):131,768
Checksum:iD93D8B4839575FDB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti431 – 4311I → V in CAA53990. (PubMed:7945253)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei301 – 31212Missing in isoform ZA and isoform ZB. 1 PublicationVSP_000406Add
BLAST
Alternative sequencei1105 – 120399RVVKV…QETPV → EVINKFQTGASFKGVLRRQN LSQQLDVKLVPSSYSEAVAS VRTSPSTSSAVTPPPVGNQS GQSIS in isoform XA and isoform ZA. 1 PublicationVSP_000407Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15408 mRNA. Translation: AAA81005.1.
U15408 mRNA. Translation: AAA81006.1.
U15408 mRNA. Translation: AAA81007.1.
U15408 mRNA. Translation: AAA81008.1.
X76452 mRNA. Translation: CAA53990.1.
L05569, L05566, L05567 Genomic DNA. Translation: AAA50820.1.
PIRiG44525.
S50027.
S54356.
S54357.
RefSeqiNP_001005871.1. NM_001005871.1. [Q64542-2]
UniGeneiRn.9986.

Genome annotation databases

GeneIDi29600.
KEGGirno:29600.
UCSCiRGD:621305. rat. [Q64542-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15408 mRNA. Translation: AAA81005.1 .
U15408 mRNA. Translation: AAA81006.1 .
U15408 mRNA. Translation: AAA81007.1 .
U15408 mRNA. Translation: AAA81008.1 .
X76452 mRNA. Translation: CAA53990.1 .
L05569 , L05566 , L05567 Genomic DNA. Translation: AAA50820.1 .
PIRi G44525.
S50027.
S54356.
S54357.
RefSeqi NP_001005871.1. NM_001005871.1. [Q64542-2 ]
UniGenei Rn.9986.

3D structure databases

ProteinModelPortali Q64542.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248232. 1 interaction.
MINTi MINT-4997403.

PTM databases

PhosphoSitei Q64542.

Proteomic databases

PaxDbi Q64542.
PRIDEi Q64542.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29600.
KEGGi rno:29600.
UCSCi RGD:621305. rat. [Q64542-1 ]

Organism-specific databases

CTDi 493.
RGDi 621305. Atp2b4.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265623.
HOVERGENi HBG061286.
InParanoidi Q64542.
KOi K05850.
PhylomeDBi Q64542.

Miscellaneous databases

NextBioi 609760.
PROi Q64542.

Gene expression databases

Genevestigatori Q64542.

Family and domain databases

Gene3Di 1.20.1110.10. 3 hits.
InterProi IPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of rat plasma membrane Ca(2+)-ATPase isoform 4 and analysis of alternative splicing patterns at splice site A."
    Keeton T.P., Shull G.E.
    Biochem. J. 306:779-785(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS XB; ZB; XA AND ZA).
    Strain: CD Charles River.
    Tissue: Testis.
  2. "Plasma-membrane calcium-pump isoforms in human and rat liver."
    Howard A., Barley N.F., Legon S., Walters J.R.F.
    Biochem. J. 303:275-279(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 266-455 (ISOFORMS XA/XB).
    Tissue: Lung.
  3. "Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
    Keeton T.P., Burk S.E., Shull G.E.
    J. Biol. Chem. 268:2740-2748(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1081-1203, ALTERNATIVE SPLICING (ISOFORMS XA/ZA).
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiAT2B4_RAT
AccessioniPrimary (citable) accession number: Q64542
Secondary accession number(s): Q63127
, Q63445, Q64543, Q64544, Q64545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3