Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q64542

- AT2B4_RAT

UniProt

Q64542 - AT2B4_RAT

Protein

Plasma membrane calcium-transporting ATPase 4

Gene

Atp2b4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

    Catalytic activityi

    ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei465 – 46514-aspartylphosphate intermediateBy similarity
    Metal bindingi785 – 7851MagnesiumBy similarity
    Metal bindingi789 – 7891MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium-transporting ATPase activity Source: RGD
    3. metal ion binding Source: UniProtKB-KW
    4. PDZ domain binding Source: RGD

    GO - Biological processi

    1. calcium ion export Source: RGD
    2. calcium ion transmembrane transport Source: RGD
    3. calcium ion transport Source: RGD
    4. cellular calcium ion homeostasis Source: RGD
    5. hippocampus development Source: RGD
    6. neural retina development Source: RGD
    7. spermatogenesis Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plasma membrane calcium-transporting ATPase 4 (EC:3.6.3.8)
    Short name:
    PMCA4
    Alternative name(s):
    Plasma membrane calcium ATPase isoform 4
    Plasma membrane calcium pump isoform 4
    Gene namesi
    Name:Atp2b4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621305. Atp2b4.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: RGD
    2. integral component of membrane Source: UniProtKB-KW
    3. neuron projection Source: RGD
    4. sperm principal piece Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12031203Plasma membrane calcium-transporting ATPase 4PRO_0000046221Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1102 – 11021Phosphothreonine; by PKCBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ64542.
    PRIDEiQ64542.

    PTM databases

    PhosphoSiteiQ64542.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Not detected in liver. The highest levels are found in uterus and stomach. Isoform XA is found in uterus, brain, stomach, small intestine, colon and pancreas. Isoform XB is found in uterus, skeletal muscle, lung, kidney, spleen, stomach, small intestine and pancreas. Isoform ZA is found in testis and isoform ZB is found in testis and heart.

    Gene expression databases

    GenevestigatoriQ64542.

    Interactioni

    Subunit structurei

    Interacts with PDZD11. Interacts with SLC35G1 and STIM1.By similarity

    Protein-protein interaction databases

    BioGridi248232. 1 interaction.
    MINTiMINT-4997403.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64542.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9292CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini114 – 15037ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini172 – 356185CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini377 – 40933ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini428 – 840413CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini861 – 87010ExtracellularSequence Analysis
    Topological domaini892 – 91120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini935 – 95218ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini975 – 99319CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1016 – 102510ExtracellularSequence Analysis
    Topological domaini1048 – 1203156CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei93 – 11321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei151 – 17121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei357 – 37620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei410 – 42718HelicalSequence AnalysisAdd
    BLAST
    Transmembranei841 – 86020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei871 – 89121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei912 – 93423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei953 – 97422HelicalSequence AnalysisAdd
    BLAST
    Transmembranei994 – 101522HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1026 – 104722HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1086 – 110318Calmodulin-binding subdomain ABy similarityAdd
    BLAST
    Regioni1104 – 111310Calmodulin-binding subdomain BBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi297 – 3037Poly-Lys

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    HOGENOMiHOG000265623.
    HOVERGENiHBG061286.
    InParanoidiQ64542.
    KOiK05850.
    PhylomeDBiQ64542.

    Family and domain databases

    Gene3Di1.20.1110.10. 3 hits.
    InterProiIPR022141. ATP_Ca_trans_C.
    IPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF12424. ATP_Ca_trans_C. 1 hit.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Note: There is a combination of two alternatively spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A and B).

    Isoform XB (identifier: Q64542-1) [UniParc]FASTAAdd to Basket

    Also known as: AIICI

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTNPSGHNLP ANSVAESYEG EFGCTLMDLR KLMELRAADA VTQISAHYGS     50
    VQEICARLKT SPVEGLSGNP ADLEKRRLVF GKNMIPPKKP KTFLELVWEA 100
    LQDVTLIILE IAAIISLVLS FYRPPGGENE ICGHIVSNPE EDEEGETGWI 150
    EGAAILASVI IVVFVTAFND WSKEKQFRGL QSRIELEQKF SIIRNGQLIQ 200
    LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT GESDHVKKTL 250
    DKDPMLLSGT HVMEGSGRMV VTAVGINSQT GIIFTLLGAN EEEDDEKKKK 300
    GKKQGVSENR NKAKTQDGVA LEIQPLNSQE GLDSEEKEKK ASKGPKKEKS 350
    VLQGKLTRLA VQIGKAGLIM SILTVLILIL YFVVDNFVIQ RRAWLPECTP 400
    VYIQYFVKFF IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL 450
    DACETMGNAT AICSDKTGTL TMNRMTVVQA YIGGTHYRQI PKPDDLPPNV 500
    LDLIVNSICI NSAYTSKILP PEKEGGLPRQ VGNKTECGLL GFVTDLKQDY 550
    QAVRSEMPEE KLFKVYTFNS VRKSMSTVIR KPEGGFRVFS KGASEIMLRK 600
    CDRILNKEGG IVPFKTKDRD NMVRNVIEPM ASEGLRTIGI AYRDFDGEEP 650
    SWENENEIFT GLVCIAVVGI EDPVRPEVPD AINKCKRAGI TVRMVTGDNV 700
    NTARAIATKC GILTPGDDFL CLEGKEFNRL IRNEKGEVEQ EKLDKVWPRL 750
    RVLARSSPTD KHTLVKGIID SNIGEQRQVV AVTGDGTNDG PALKKADVGF 800
    AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI SKFLQFQLTV 850
    NVVAVIVAFS GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTDSLL 900
    RRRPYGRNKP LISRTMMKNI LGHAVYQLGI VFLLVFAGDK LFDIDSGRKA 950
    PLNSPPSQHY TIVFNTFVLM QLFNEINSRK IHGEKNVFAG VYRNIIFCSV 1000
    VLGTFFCQIL IVEVGGKPFS CTNLTMEQWM WCLFIGIGEL LWGQVISAIP 1050
    TKSLKFLKEA GHGSDKEEIS KDAEGLEEID HAEMELRRGQ ILWVRGLNRI 1100
    QTQIRVVKVF HSFRDVIHKS KNQVSIHSFM TQPEYAADDE MSQSFLNQEE 1150
    SPSLASKSRI TKRLSDAETV SQNNTNNNAV DCHQVQIVAS HPNSPLQSQE 1200
    TPV 1203
    Length:1,203
    Mass (Da):133,094
    Last modified:November 1, 1996 - v1
    Checksum:iB7A6C8D4556F6398
    GO
    Isoform XA (identifier: Q64542-2) [UniParc]FASTAAdd to Basket

    Also known as: AIICII

    The sequence of this isoform differs from the canonical sequence as follows:
         1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS

    Show »
    Length:1,169
    Mass (Da):128,791
    Checksum:iC799FA6AC708EBE4
    GO
    Isoform ZA (identifier: Q64542-3) [UniParc]FASTAAdd to Basket

    Also known as: AICII

    The sequence of this isoform differs from the canonical sequence as follows:
         301-312: Missing.
         1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS

    Show »
    Length:1,157
    Mass (Da):127,464
    Checksum:iEDB410076D59B247
    GO
    Isoform ZB (identifier: Q64542-4) [UniParc]FASTAAdd to Basket

    Also known as: AICI

    The sequence of this isoform differs from the canonical sequence as follows:
         301-312: Missing.

    Show »
    Length:1,191
    Mass (Da):131,768
    Checksum:iD93D8B4839575FDB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti431 – 4311I → V in CAA53990. (PubMed:7945253)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei301 – 31212Missing in isoform ZA and isoform ZB. 1 PublicationVSP_000406Add
    BLAST
    Alternative sequencei1105 – 120399RVVKV…QETPV → EVINKFQTGASFKGVLRRQN LSQQLDVKLVPSSYSEAVAS VRTSPSTSSAVTPPPVGNQS GQSIS in isoform XA and isoform ZA. 1 PublicationVSP_000407Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15408 mRNA. Translation: AAA81005.1.
    U15408 mRNA. Translation: AAA81006.1.
    U15408 mRNA. Translation: AAA81007.1.
    U15408 mRNA. Translation: AAA81008.1.
    X76452 mRNA. Translation: CAA53990.1.
    L05569, L05566, L05567 Genomic DNA. Translation: AAA50820.1.
    PIRiG44525.
    S50027.
    S54356.
    S54357.
    RefSeqiNP_001005871.1. NM_001005871.1. [Q64542-2]
    UniGeneiRn.9986.

    Genome annotation databases

    GeneIDi29600.
    KEGGirno:29600.
    UCSCiRGD:621305. rat. [Q64542-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15408 mRNA. Translation: AAA81005.1 .
    U15408 mRNA. Translation: AAA81006.1 .
    U15408 mRNA. Translation: AAA81007.1 .
    U15408 mRNA. Translation: AAA81008.1 .
    X76452 mRNA. Translation: CAA53990.1 .
    L05569 , L05566 , L05567 Genomic DNA. Translation: AAA50820.1 .
    PIRi G44525.
    S50027.
    S54356.
    S54357.
    RefSeqi NP_001005871.1. NM_001005871.1. [Q64542-2 ]
    UniGenei Rn.9986.

    3D structure databases

    ProteinModelPortali Q64542.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248232. 1 interaction.
    MINTi MINT-4997403.

    PTM databases

    PhosphoSitei Q64542.

    Proteomic databases

    PaxDbi Q64542.
    PRIDEi Q64542.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29600.
    KEGGi rno:29600.
    UCSCi RGD:621305. rat. [Q64542-1 ]

    Organism-specific databases

    CTDi 493.
    RGDi 621305. Atp2b4.

    Phylogenomic databases

    eggNOGi COG0474.
    HOGENOMi HOG000265623.
    HOVERGENi HBG061286.
    InParanoidi Q64542.
    KOi K05850.
    PhylomeDBi Q64542.

    Miscellaneous databases

    NextBioi 609760.
    PROi Q64542.

    Gene expression databases

    Genevestigatori Q64542.

    Family and domain databases

    Gene3Di 1.20.1110.10. 3 hits.
    InterProi IPR022141. ATP_Ca_trans_C.
    IPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF12424. ATP_Ca_trans_C. 1 hit.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of rat plasma membrane Ca(2+)-ATPase isoform 4 and analysis of alternative splicing patterns at splice site A."
      Keeton T.P., Shull G.E.
      Biochem. J. 306:779-785(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS XB; ZB; XA AND ZA).
      Strain: CD Charles River.
      Tissue: Testis.
    2. "Plasma-membrane calcium-pump isoforms in human and rat liver."
      Howard A., Barley N.F., Legon S., Walters J.R.F.
      Biochem. J. 303:275-279(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 266-455 (ISOFORMS XA/XB).
      Tissue: Lung.
    3. "Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
      Keeton T.P., Burk S.E., Shull G.E.
      J. Biol. Chem. 268:2740-2748(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1081-1203, ALTERNATIVE SPLICING (ISOFORMS XA/ZA).
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiAT2B4_RAT
    AccessioniPrimary (citable) accession number: Q64542
    Secondary accession number(s): Q63127
    , Q63445, Q64543, Q64544, Q64545
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3