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Q64542 (AT2B4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasma membrane calcium-transporting ATPase 4

Short name=PMCA4
EC=3.6.3.8
Alternative name(s):
Plasma membrane calcium ATPase isoform 4
Plasma membrane calcium pump isoform 4
Gene names
Name:Atp2b4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1203 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activity

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Subunit structure

Interacts with PDZD11 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Ubiquitously expressed. Not detected in liver. The highest levels are found in uterus and stomach. Isoform XA is found in uterus, brain, stomach, small intestine, colon and pancreas. Isoform XB is found in uterus, skeletal muscle, lung, kidney, spleen, stomach, small intestine and pancreas. Isoform ZA is found in testis and isoform ZB is found in testis and heart.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Calcium
Calmodulin-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcalcium ion export

Inferred from mutant phenotype PubMed 9227424. Source: RGD

calcium ion transmembrane transport

Inferred from mutant phenotype PubMed 21325503. Source: RGD

calcium ion transport

Inferred from direct assay PubMed 11779484. Source: RGD

cellular calcium ion homeostasis

Traceable author statement PubMed 12511555. Source: RGD

hippocampus development

Inferred from expression pattern PubMed 16200642. Source: RGD

neural retina development

Inferred from expression pattern PubMed 16079002. Source: RGD

spermatogenesis

Inferred from expression pattern PubMed 18057950. Source: RGD

   Cellular_componentbasolateral plasma membrane

Inferred from direct assay PubMed 18057950. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuron projection

Inferred from direct assay PubMed 16200642. Source: RGD

sperm principal piece

Inferred from direct assay PubMed 18057950. Source: RGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

PDZ domain binding

Inferred from physical interaction PubMed 16554037. Source: RGD

calcium-transporting ATPase activity

Inferred from direct assay PubMed 11779484. Source: RGD

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: There is a combination of two alternatively spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A and B).
Isoform XB (identifier: Q64542-1)

Also known as: AIICI;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform XA (identifier: Q64542-2)

Also known as: AIICII;

The sequence of this isoform differs from the canonical sequence as follows:
     1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS
Isoform ZA (identifier: Q64542-3)

Also known as: AICII;

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.
     1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS
Isoform ZB (identifier: Q64542-4)

Also known as: AICI;

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12031203Plasma membrane calcium-transporting ATPase 4
PRO_0000046221

Regions

Topological domain1 – 9292Cytoplasmic Potential
Transmembrane93 – 11321Helical; Potential
Topological domain114 – 15037Extracellular Potential
Transmembrane151 – 17121Helical; Potential
Topological domain172 – 356185Cytoplasmic Potential
Transmembrane357 – 37620Helical; Potential
Topological domain377 – 40933Extracellular Potential
Transmembrane410 – 42718Helical; Potential
Topological domain428 – 840413Cytoplasmic Potential
Transmembrane841 – 86020Helical; Potential
Topological domain861 – 87010Extracellular Potential
Transmembrane871 – 89121Helical; Potential
Topological domain892 – 91120Cytoplasmic Potential
Transmembrane912 – 93423Helical; Potential
Topological domain935 – 95218Extracellular Potential
Transmembrane953 – 97422Helical; Potential
Topological domain975 – 99319Cytoplasmic Potential
Transmembrane994 – 101522Helical; Potential
Topological domain1016 – 102510Extracellular Potential
Transmembrane1026 – 104722Helical; Potential
Topological domain1048 – 1203156Cytoplasmic Potential
Region1086 – 110318Calmodulin-binding subdomain A By similarity
Region1104 – 111310Calmodulin-binding subdomain B By similarity
Compositional bias297 – 3037Poly-Lys

Sites

Active site46514-aspartylphosphate intermediate By similarity
Metal binding7851Magnesium By similarity
Metal binding7891Magnesium By similarity

Amino acid modifications

Modified residue11021Phosphothreonine; by PKC By similarity

Natural variations

Alternative sequence301 – 31212Missing in isoform ZA and isoform ZB.
VSP_000406
Alternative sequence1105 – 120399RVVKV…QETPV → EVINKFQTGASFKGVLRRQN LSQQLDVKLVPSSYSEAVAS VRTSPSTSSAVTPPPVGNQS GQSIS in isoform XA and isoform ZA.
VSP_000407

Experimental info

Sequence conflict4311I → V in CAA53990. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform XB (AIICI) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B7A6C8D4556F6398

FASTA1,203133,094
        10         20         30         40         50         60 
MTNPSGHNLP ANSVAESYEG EFGCTLMDLR KLMELRAADA VTQISAHYGS VQEICARLKT 

        70         80         90        100        110        120 
SPVEGLSGNP ADLEKRRLVF GKNMIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 

       130        140        150        160        170        180 
FYRPPGGENE ICGHIVSNPE EDEEGETGWI EGAAILASVI IVVFVTAFND WSKEKQFRGL 

       190        200        210        220        230        240 
QSRIELEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 

       250        260        270        280        290        300 
GESDHVKKTL DKDPMLLSGT HVMEGSGRMV VTAVGINSQT GIIFTLLGAN EEEDDEKKKK 

       310        320        330        340        350        360 
GKKQGVSENR NKAKTQDGVA LEIQPLNSQE GLDSEEKEKK ASKGPKKEKS VLQGKLTRLA 

       370        380        390        400        410        420 
VQIGKAGLIM SILTVLILIL YFVVDNFVIQ RRAWLPECTP VYIQYFVKFF IIGVTVLVVA 

       430        440        450        460        470        480 
VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA 

       490        500        510        520        530        540 
YIGGTHYRQI PKPDDLPPNV LDLIVNSICI NSAYTSKILP PEKEGGLPRQ VGNKTECGLL 

       550        560        570        580        590        600 
GFVTDLKQDY QAVRSEMPEE KLFKVYTFNS VRKSMSTVIR KPEGGFRVFS KGASEIMLRK 

       610        620        630        640        650        660 
CDRILNKEGG IVPFKTKDRD NMVRNVIEPM ASEGLRTIGI AYRDFDGEEP SWENENEIFT 

       670        680        690        700        710        720 
GLVCIAVVGI EDPVRPEVPD AINKCKRAGI TVRMVTGDNV NTARAIATKC GILTPGDDFL 

       730        740        750        760        770        780 
CLEGKEFNRL IRNEKGEVEQ EKLDKVWPRL RVLARSSPTD KHTLVKGIID SNIGEQRQVV 

       790        800        810        820        830        840 
AVTGDGTNDG PALKKADVGF AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI 

       850        860        870        880        890        900 
SKFLQFQLTV NVVAVIVAFS GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTDSLL 

       910        920        930        940        950        960 
RRRPYGRNKP LISRTMMKNI LGHAVYQLGI VFLLVFAGDK LFDIDSGRKA PLNSPPSQHY 

       970        980        990       1000       1010       1020 
TIVFNTFVLM QLFNEINSRK IHGEKNVFAG VYRNIIFCSV VLGTFFCQIL IVEVGGKPFS 

      1030       1040       1050       1060       1070       1080 
CTNLTMEQWM WCLFIGIGEL LWGQVISAIP TKSLKFLKEA GHGSDKEEIS KDAEGLEEID 

      1090       1100       1110       1120       1130       1140 
HAEMELRRGQ ILWVRGLNRI QTQIRVVKVF HSFRDVIHKS KNQVSIHSFM TQPEYAADDE 

      1150       1160       1170       1180       1190       1200 
MSQSFLNQEE SPSLASKSRI TKRLSDAETV SQNNTNNNAV DCHQVQIVAS HPNSPLQSQE 


TPV 

« Hide

Isoform XA (AIICII) [UniParc].

Checksum: C799FA6AC708EBE4
Show »

FASTA1,169128,791
Isoform ZA (AICII) [UniParc].

Checksum: EDB410076D59B247
Show »

FASTA1,157127,464
Isoform ZB (AICI) [UniParc].

Checksum: D93D8B4839575FDB
Show »

FASTA1,191131,768

References

[1]"Primary structure of rat plasma membrane Ca(2+)-ATPase isoform 4 and analysis of alternative splicing patterns at splice site A."
Keeton T.P., Shull G.E.
Biochem. J. 306:779-785(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS XB; ZB; XA AND ZA).
Strain: CD Charles River.
Tissue: Testis.
[2]"Plasma-membrane calcium-pump isoforms in human and rat liver."
Howard A., Barley N.F., Legon S., Walters J.R.F.
Biochem. J. 303:275-279(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 266-455 (ISOFORMS XA/XB).
Tissue: Lung.
[3]"Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
Keeton T.P., Burk S.E., Shull G.E.
J. Biol. Chem. 268:2740-2748(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1081-1203, ALTERNATIVE SPLICING (ISOFORMS XA/ZA).
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15408 mRNA. Translation: AAA81005.1.
U15408 mRNA. Translation: AAA81006.1.
U15408 mRNA. Translation: AAA81007.1.
U15408 mRNA. Translation: AAA81008.1.
X76452 mRNA. Translation: CAA53990.1.
L05569, L05566, L05567 Genomic DNA. Translation: AAA50820.1.
PIRG44525.
S50027.
S54356.
S54357.
RefSeqNP_001005871.1. NM_001005871.1. [Q64542-2]
UniGeneRn.9986.

3D structure databases

ProteinModelPortalQ64542.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248232. 1 interaction.
MINTMINT-4997403.

PTM databases

PhosphoSiteQ64542.

Proteomic databases

PaxDbQ64542.
PRIDEQ64542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29600.
KEGGrno:29600.
UCSCRGD:621305. rat. [Q64542-1]

Organism-specific databases

CTD493.
RGD621305. Atp2b4.

Phylogenomic databases

eggNOGCOG0474.
HOGENOMHOG000265623.
HOVERGENHBG061286.
InParanoidQ64542.
KOK05850.
PhylomeDBQ64542.

Gene expression databases

GenevestigatorQ64542.

Family and domain databases

Gene3D1.20.1110.10. 3 hits.
InterProIPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio609760.
PROQ64542.

Entry information

Entry nameAT2B4_RAT
AccessionPrimary (citable) accession number: Q64542
Secondary accession number(s): Q63127 expand/collapse secondary AC list , Q63445, Q64543, Q64544, Q64545
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families