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Q64541 (AT1A4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/potassium-transporting ATPase subunit alpha-4

Short name=Na(+)/K(+) ATPase alpha-4 subunit
EC=3.6.3.9
Alternative name(s):
Sodium pump subunit alpha-4
Gene names
Name:Atp1a4
Synonyms:Atp1al2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1028 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility. Ref.2

Catalytic activity

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Enzyme regulation

Specifically inhibited by an endogenous cardiac glycoside, ouabain By similarity.

Subunit structure

COmposed of three subunits: alpha (catalytic), beta and gamma.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Sodium transport
Sodium/potassium transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
Sodium
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP biosynthetic process

Inferred from electronic annotation. Source: InterPro

fertilization

Inferred from electronic annotation. Source: Ensembl

potassium ion transport

Traceable author statement PubMed 12112599. Source: RGD

regulation of cellular pH

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of membrane potential

Inferred from electronic annotation. Source: Ensembl

sodium ion transmembrane transport

Inferred from direct assay PubMed 10555956. Source: GOC

sodium ion transport

Traceable author statement PubMed 12112599. Source: RGD

sperm motility

Inferred from sequence or structural similarity. Source: UniProtKB

spermatogenesis

Inferred from expression pattern PubMed 16264093. Source: RGD

   Cellular_componentsodium:potassium-exchanging ATPase complex

Traceable author statement PubMed 12112599. Source: RGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sodium:potassium-exchanging ATPase activity

Inferred from direct assay PubMed 10555956. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10281028Sodium/potassium-transporting ATPase subunit alpha-4
PRO_0000046305

Regions

Topological domain1 – 9292Cytoplasmic Potential
Transmembrane93 – 11321Helical; Potential
Topological domain114 – 13724Extracellular Potential
Transmembrane138 – 15821Helical; Potential
Topological domain159 – 294136Cytoplasmic Potential
Transmembrane295 – 31420Helical; Potential
Topological domain315 – 32612Extracellular Potential
Transmembrane327 – 34418Helical; Potential
Topological domain345 – 777433Cytoplasmic Potential
Transmembrane778 – 79720Helical; Potential
Topological domain798 – 80710Extracellular Potential
Transmembrane808 – 82821Helical; Potential
Topological domain829 – 84820Cytoplasmic Potential
Transmembrane849 – 87123Helical; Potential
Topological domain872 – 92352Extracellular Potential
Transmembrane924 – 94320Helical; Potential
Topological domain944 – 95613Cytoplasmic Potential
Transmembrane957 – 97519Helical; Potential
Topological domain976 – 99015Extracellular Potential
Transmembrane991 – 101121Helical; Potential
Topological domain1012 – 102817Cytoplasmic Potential
Region87 – 893Interaction with phosphoinositide-3 kinase By similarity

Sites

Active site38214-aspartylphosphate intermediate By similarity
Metal binding7221Magnesium By similarity
Metal binding7261Magnesium By similarity

Amino acid modifications

Modified residue9481Phosphoserine; by PKA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64541 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 858FE008735D06FA

FASTA1,028114,005
        10         20         30         40         50         60 
MEPGKETAAT SEQKPRPTLR ASNTNRQPKV KRRKKDLEEL KKEVVMDDHK LTLDELSAKY 

        70         80         90        100        110        120 
SVDLTKGLSV TDAQEILTLN GPNVLTPPPT TPEWIKFCKQ LFGGFSLLLW TGSLLCFLAY 

       130        140        150        160        170        180 
GIHVSYYQEN ANKDNLYLGI VLSAVVIITG CFSYYQEAKS SKIMESFKTM VPQQALVIRD 

       190        200        210        220        230        240 
GEKMQINVRD VVLGDLVEVK GGDQVPADIR VIAAQGCKVD NSSLTGESEP QSRCPDCTHE 

       250        260        270        280        290        300 
NPLETRNIIF FSTNCVEGTA RGVVIATGDH TVMGRIASLT SGLTMGKTPI ATEIEHFIHI 

       310        320        330        340        350        360 
ITAVAVFLGV TFFFLSLILG YTWLDAVIFL IGIIVANVPE GLLATVTVCL TLTAKRMARK 

       370        380        390        400        410        420 
NCLVKNLEAV ETLGSTSTIC SDKTGTLTQN RMTVAHLWFD KTVYEADTSE EQTTGKTFPK 

       430        440        450        460        470        480 
SSDTWFYLAR IAGLCNRADF KPHQESLPIT KRTTTGDASE SALLKFIEQS YSPVSEMRQK 

       490        500        510        520        530        540 
NPKVAEIPFN STNKYQMSIH LLEDNSEAHV LLMKGAPERI LDFCSSFLLN GQEYPMDEEM 

       550        560        570        580        590        600 
KTDFQNAYIE LGGLGERVLG FCFLNLPSNF SKGFQFNTEE LNFPMENLCF AGLISMIDPP 

       610        620        630        640        650        660 
RTAVPDAVSK CRSAGIKVIM VTGDHPITAK AIAKSVGIIS EANETAEDIA ARLNISISQV 

       670        680        690        700        710        720 
SNKSIKAIVV HGSELKDMDS GQLDNILKSY KEIVFARTSP QQKLIIVEGC QRLGAIVAVT 

       730        740        750        760        770        780 
GDGVNDSPAL KKADIGIAMG ITGSDVSKQA ADMILLDDNF ASIVTGVEEG RLIFDNLKKS 

       790        800        810        820        830        840 
IAYTLTSNIP EITPFLLFIV LSIPLPLGTI TILCIDLGTD MVPAISLAYE TPESDIMKRL 

       850        860        870        880        890        900 
PRNPKTDNLV NDRLIGMAYG QIGMIQALAG FFTYFVILAE NGFKPLDLLG IRLYWDDTNL 

       910        920        930        940        950        960 
NDLEDTYGQQ WTYEQRKVVE FTCQTAFFIS IVIVQWADLI ICKTRRNSLF KQGMKNKVLI 

       970        980        990       1000       1010       1020 
FGLLEETILA ACLSYIPGMD VALRMYPLKI NWWFCALPYS VLIFIYDEVR KLIIRRRPGG 


WLEKETYY 

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References

[1]"A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed in testis."
Shamraj O.I., Lingrel J.B.
Proc. Natl. Acad. Sci. U.S.A. 91:12952-12956(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Testis.
[2]"Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa."
Jimenez T., Sanchez G., Wertheimer E., Blanco G.
Reproduction 139:835-845(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15176 mRNA. Translation: AAB81285.1.
RefSeqNP_001257959.1. NM_001271030.1.
NP_074039.1. NM_022848.3.
UniGeneRn.224462.

3D structure databases

ProteinModelPortalQ64541.
SMRQ64541. Positions 32-1028.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4997397.

PTM databases

PhosphoSiteQ64541.

Proteomic databases

PaxDbQ64541.
PRIDEQ64541.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000064601; ENSRNOP00000062079; ENSRNOG00000032378.
GeneID29132.
KEGGrno:29132.
UCSCRGD:61952. rat.

Organism-specific databases

CTD480.
RGD61952. Atp1a4.

Phylogenomic databases

eggNOGCOG0474.
GeneTreeENSGT00560000076866.
HOVERGENHBG004298.
KOK01539.
OMAKSSDTWF.
OrthoDBEOG7327N0.
PhylomeDBQ64541.

Enzyme and pathway databases

SABIO-RKQ64541.

Gene expression databases

GenevestigatorQ64541.

Family and domain databases

Gene3D1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 4 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608083.

Entry information

Entry nameAT1A4_RAT
AccessionPrimary (citable) accession number: Q64541
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families