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Protein

Sodium/potassium-transporting ATPase subunit alpha-4

Gene

Atp1a4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility.1 Publication

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Enzyme regulationi

Specifically inhibited by an endogenous cardiac glycoside, ouabain.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei382 – 38214-aspartylphosphate intermediateBy similarity
Metal bindingi722 – 7221MagnesiumBy similarity
Metal bindingi726 – 7261MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • sodium:potassium-exchanging ATPase activity Source: MGI

GO - Biological processi

  • fertilization Source: Ensembl
  • potassium ion transport Source: RGD
  • regulation of cellular pH Source: UniProtKB
  • regulation of membrane potential Source: Ensembl
  • sodium ion transmembrane transport Source: GOC
  • sodium ion transport Source: RGD
  • spermatogenesis Source: RGD
  • sperm motility Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiREACT_345620. Ion transport by P-type ATPases.
SABIO-RKQ64541.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-4 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-4 subunit
Alternative name(s):
Sodium pump subunit alpha-4
Gene namesi
Name:Atp1a4
Synonyms:Atp1al2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi61952. Atp1a4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9292CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei93 – 11321HelicalSequence AnalysisAdd
BLAST
Topological domaini114 – 13724ExtracellularSequence AnalysisAdd
BLAST
Transmembranei138 – 15821HelicalSequence AnalysisAdd
BLAST
Topological domaini159 – 294136CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei295 – 31420HelicalSequence AnalysisAdd
BLAST
Topological domaini315 – 32612ExtracellularSequence AnalysisAdd
BLAST
Transmembranei327 – 34418HelicalSequence AnalysisAdd
BLAST
Topological domaini345 – 777433CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei778 – 79720HelicalSequence AnalysisAdd
BLAST
Topological domaini798 – 80710ExtracellularSequence Analysis
Transmembranei808 – 82821HelicalSequence AnalysisAdd
BLAST
Topological domaini829 – 84820CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei849 – 87123HelicalSequence AnalysisAdd
BLAST
Topological domaini872 – 92352ExtracellularSequence AnalysisAdd
BLAST
Transmembranei924 – 94320HelicalSequence AnalysisAdd
BLAST
Topological domaini944 – 95613CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei957 – 97519HelicalSequence AnalysisAdd
BLAST
Topological domaini976 – 99015ExtracellularSequence AnalysisAdd
BLAST
Transmembranei991 – 101121HelicalSequence AnalysisAdd
BLAST
Topological domaini1012 – 102817CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • sodium:potassium-exchanging ATPase complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10281028Sodium/potassium-transporting ATPase subunit alpha-4PRO_0000046305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei948 – 9481Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64541.
PRIDEiQ64541.

PTM databases

PhosphoSiteiQ64541.

Expressioni

Gene expression databases

ExpressionAtlasiQ64541. baseline.
GenevisibleiQ64541. RN.

Interactioni

Subunit structurei

COmposed of three subunits: alpha (catalytic), beta and gamma.

Protein-protein interaction databases

MINTiMINT-4997397.
STRINGi10116.ENSRNOP00000062079.

Structurei

3D structure databases

ProteinModelPortaliQ64541.
SMRiQ64541. Positions 32-1028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 893Interaction with phosphoinositide-3 kinaseBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00760000119003.
HOVERGENiHBG004298.
InParanoidiQ64541.
KOiK01539.
OMAiKSSDTWF.
OrthoDBiEOG7327N0.
PhylomeDBiQ64541.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 4 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPGKETAAT SEQKPRPTLR ASNTNRQPKV KRRKKDLEEL KKEVVMDDHK
60 70 80 90 100
LTLDELSAKY SVDLTKGLSV TDAQEILTLN GPNVLTPPPT TPEWIKFCKQ
110 120 130 140 150
LFGGFSLLLW TGSLLCFLAY GIHVSYYQEN ANKDNLYLGI VLSAVVIITG
160 170 180 190 200
CFSYYQEAKS SKIMESFKTM VPQQALVIRD GEKMQINVRD VVLGDLVEVK
210 220 230 240 250
GGDQVPADIR VIAAQGCKVD NSSLTGESEP QSRCPDCTHE NPLETRNIIF
260 270 280 290 300
FSTNCVEGTA RGVVIATGDH TVMGRIASLT SGLTMGKTPI ATEIEHFIHI
310 320 330 340 350
ITAVAVFLGV TFFFLSLILG YTWLDAVIFL IGIIVANVPE GLLATVTVCL
360 370 380 390 400
TLTAKRMARK NCLVKNLEAV ETLGSTSTIC SDKTGTLTQN RMTVAHLWFD
410 420 430 440 450
KTVYEADTSE EQTTGKTFPK SSDTWFYLAR IAGLCNRADF KPHQESLPIT
460 470 480 490 500
KRTTTGDASE SALLKFIEQS YSPVSEMRQK NPKVAEIPFN STNKYQMSIH
510 520 530 540 550
LLEDNSEAHV LLMKGAPERI LDFCSSFLLN GQEYPMDEEM KTDFQNAYIE
560 570 580 590 600
LGGLGERVLG FCFLNLPSNF SKGFQFNTEE LNFPMENLCF AGLISMIDPP
610 620 630 640 650
RTAVPDAVSK CRSAGIKVIM VTGDHPITAK AIAKSVGIIS EANETAEDIA
660 670 680 690 700
ARLNISISQV SNKSIKAIVV HGSELKDMDS GQLDNILKSY KEIVFARTSP
710 720 730 740 750
QQKLIIVEGC QRLGAIVAVT GDGVNDSPAL KKADIGIAMG ITGSDVSKQA
760 770 780 790 800
ADMILLDDNF ASIVTGVEEG RLIFDNLKKS IAYTLTSNIP EITPFLLFIV
810 820 830 840 850
LSIPLPLGTI TILCIDLGTD MVPAISLAYE TPESDIMKRL PRNPKTDNLV
860 870 880 890 900
NDRLIGMAYG QIGMIQALAG FFTYFVILAE NGFKPLDLLG IRLYWDDTNL
910 920 930 940 950
NDLEDTYGQQ WTYEQRKVVE FTCQTAFFIS IVIVQWADLI ICKTRRNSLF
960 970 980 990 1000
KQGMKNKVLI FGLLEETILA ACLSYIPGMD VALRMYPLKI NWWFCALPYS
1010 1020
VLIFIYDEVR KLIIRRRPGG WLEKETYY
Length:1,028
Mass (Da):114,005
Last modified:November 1, 1996 - v1
Checksum:i858FE008735D06FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15176 mRNA. Translation: AAB81285.1.
RefSeqiNP_001257959.1. NM_001271030.1.
NP_074039.1. NM_022848.3.
UniGeneiRn.224462.

Genome annotation databases

EnsembliENSRNOT00000064601; ENSRNOP00000062079; ENSRNOG00000032378.
GeneIDi29132.
KEGGirno:29132.
UCSCiRGD:61952. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15176 mRNA. Translation: AAB81285.1.
RefSeqiNP_001257959.1. NM_001271030.1.
NP_074039.1. NM_022848.3.
UniGeneiRn.224462.

3D structure databases

ProteinModelPortaliQ64541.
SMRiQ64541. Positions 32-1028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997397.
STRINGi10116.ENSRNOP00000062079.

PTM databases

PhosphoSiteiQ64541.

Proteomic databases

PaxDbiQ64541.
PRIDEiQ64541.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000064601; ENSRNOP00000062079; ENSRNOG00000032378.
GeneIDi29132.
KEGGirno:29132.
UCSCiRGD:61952. rat.

Organism-specific databases

CTDi480.
RGDi61952. Atp1a4.

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00760000119003.
HOVERGENiHBG004298.
InParanoidiQ64541.
KOiK01539.
OMAiKSSDTWF.
OrthoDBiEOG7327N0.
PhylomeDBiQ64541.

Enzyme and pathway databases

ReactomeiREACT_345620. Ion transport by P-type ATPases.
SABIO-RKQ64541.

Miscellaneous databases

NextBioi608083.
PROiQ64541.

Gene expression databases

ExpressionAtlasiQ64541. baseline.
GenevisibleiQ64541. RN.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 4 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed in testis."
    Shamraj O.I., Lingrel J.B.
    Proc. Natl. Acad. Sci. U.S.A. 91:12952-12956(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa."
    Jimenez T., Sanchez G., Wertheimer E., Blanco G.
    Reproduction 139:835-845(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAT1A4_RAT
AccessioniPrimary (citable) accession number: Q64541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.