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Q64541

- AT1A4_RAT

UniProt

Q64541 - AT1A4_RAT

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Protein
Sodium/potassium-transporting ATPase subunit alpha-4
Gene
Atp1a4, Atp1al2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility.1 Publication

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Enzyme regulationi

Specifically inhibited by an endogenous cardiac glycoside, ouabain By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei382 – 38214-aspartylphosphate intermediate By similarity
Metal bindingi722 – 7221Magnesium By similarity
Metal bindingi726 – 7261Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. sodium:potassium-exchanging ATPase activity Source: MGI

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. fertilization Source: Ensembl
  3. potassium ion transport Source: RGD
  4. regulation of cellular pH Source: UniProtKB
  5. regulation of membrane potential Source: Ensembl
  6. sodium ion transmembrane transport Source: GOC
  7. sodium ion transport Source: RGD
  8. sperm motility Source: UniProtKB
  9. spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

SABIO-RKQ64541.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-4 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-4 subunit
Alternative name(s):
Sodium pump subunit alpha-4
Gene namesi
Name:Atp1a4
Synonyms:Atp1al2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi61952. Atp1a4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9292Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei93 – 11321Helical; Reviewed prediction
Add
BLAST
Topological domaini114 – 13724Extracellular Reviewed prediction
Add
BLAST
Transmembranei138 – 15821Helical; Reviewed prediction
Add
BLAST
Topological domaini159 – 294136Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei295 – 31420Helical; Reviewed prediction
Add
BLAST
Topological domaini315 – 32612Extracellular Reviewed prediction
Add
BLAST
Transmembranei327 – 34418Helical; Reviewed prediction
Add
BLAST
Topological domaini345 – 777433Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei778 – 79720Helical; Reviewed prediction
Add
BLAST
Topological domaini798 – 80710Extracellular Reviewed prediction
Transmembranei808 – 82821Helical; Reviewed prediction
Add
BLAST
Topological domaini829 – 84820Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei849 – 87123Helical; Reviewed prediction
Add
BLAST
Topological domaini872 – 92352Extracellular Reviewed prediction
Add
BLAST
Transmembranei924 – 94320Helical; Reviewed prediction
Add
BLAST
Topological domaini944 – 95613Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei957 – 97519Helical; Reviewed prediction
Add
BLAST
Topological domaini976 – 99015Extracellular Reviewed prediction
Add
BLAST
Transmembranei991 – 101121Helical; Reviewed prediction
Add
BLAST
Topological domaini1012 – 102817Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. sodium:potassium-exchanging ATPase complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10281028Sodium/potassium-transporting ATPase subunit alpha-4
PRO_0000046305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei948 – 9481Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64541.
PRIDEiQ64541.

PTM databases

PhosphoSiteiQ64541.

Expressioni

Gene expression databases

GenevestigatoriQ64541.

Interactioni

Subunit structurei

COmposed of three subunits: alpha (catalytic), beta and gamma.

Protein-protein interaction databases

MINTiMINT-4997397.

Structurei

3D structure databases

ProteinModelPortaliQ64541.
SMRiQ64541. Positions 32-1028.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 893Interaction with phosphoinositide-3 kinase By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00560000076866.
HOVERGENiHBG004298.
KOiK01539.
OMAiKSSDTWF.
OrthoDBiEOG7327N0.
PhylomeDBiQ64541.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 4 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64541-1 [UniParc]FASTAAdd to Basket

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MEPGKETAAT SEQKPRPTLR ASNTNRQPKV KRRKKDLEEL KKEVVMDDHK     50
LTLDELSAKY SVDLTKGLSV TDAQEILTLN GPNVLTPPPT TPEWIKFCKQ 100
LFGGFSLLLW TGSLLCFLAY GIHVSYYQEN ANKDNLYLGI VLSAVVIITG 150
CFSYYQEAKS SKIMESFKTM VPQQALVIRD GEKMQINVRD VVLGDLVEVK 200
GGDQVPADIR VIAAQGCKVD NSSLTGESEP QSRCPDCTHE NPLETRNIIF 250
FSTNCVEGTA RGVVIATGDH TVMGRIASLT SGLTMGKTPI ATEIEHFIHI 300
ITAVAVFLGV TFFFLSLILG YTWLDAVIFL IGIIVANVPE GLLATVTVCL 350
TLTAKRMARK NCLVKNLEAV ETLGSTSTIC SDKTGTLTQN RMTVAHLWFD 400
KTVYEADTSE EQTTGKTFPK SSDTWFYLAR IAGLCNRADF KPHQESLPIT 450
KRTTTGDASE SALLKFIEQS YSPVSEMRQK NPKVAEIPFN STNKYQMSIH 500
LLEDNSEAHV LLMKGAPERI LDFCSSFLLN GQEYPMDEEM KTDFQNAYIE 550
LGGLGERVLG FCFLNLPSNF SKGFQFNTEE LNFPMENLCF AGLISMIDPP 600
RTAVPDAVSK CRSAGIKVIM VTGDHPITAK AIAKSVGIIS EANETAEDIA 650
ARLNISISQV SNKSIKAIVV HGSELKDMDS GQLDNILKSY KEIVFARTSP 700
QQKLIIVEGC QRLGAIVAVT GDGVNDSPAL KKADIGIAMG ITGSDVSKQA 750
ADMILLDDNF ASIVTGVEEG RLIFDNLKKS IAYTLTSNIP EITPFLLFIV 800
LSIPLPLGTI TILCIDLGTD MVPAISLAYE TPESDIMKRL PRNPKTDNLV 850
NDRLIGMAYG QIGMIQALAG FFTYFVILAE NGFKPLDLLG IRLYWDDTNL 900
NDLEDTYGQQ WTYEQRKVVE FTCQTAFFIS IVIVQWADLI ICKTRRNSLF 950
KQGMKNKVLI FGLLEETILA ACLSYIPGMD VALRMYPLKI NWWFCALPYS 1000
VLIFIYDEVR KLIIRRRPGG WLEKETYY 1028
Length:1,028
Mass (Da):114,005
Last modified:November 1, 1996 - v1
Checksum:i858FE008735D06FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15176 mRNA. Translation: AAB81285.1.
RefSeqiNP_001257959.1. NM_001271030.1.
NP_074039.1. NM_022848.3.
UniGeneiRn.224462.

Genome annotation databases

EnsembliENSRNOT00000064601; ENSRNOP00000062079; ENSRNOG00000032378.
GeneIDi29132.
KEGGirno:29132.
UCSCiRGD:61952. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15176 mRNA. Translation: AAB81285.1 .
RefSeqi NP_001257959.1. NM_001271030.1.
NP_074039.1. NM_022848.3.
UniGenei Rn.224462.

3D structure databases

ProteinModelPortali Q64541.
SMRi Q64541. Positions 32-1028.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4997397.

PTM databases

PhosphoSitei Q64541.

Proteomic databases

PaxDbi Q64541.
PRIDEi Q64541.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000064601 ; ENSRNOP00000062079 ; ENSRNOG00000032378 .
GeneIDi 29132.
KEGGi rno:29132.
UCSCi RGD:61952. rat.

Organism-specific databases

CTDi 480.
RGDi 61952. Atp1a4.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00560000076866.
HOVERGENi HBG004298.
KOi K01539.
OMAi KSSDTWF.
OrthoDBi EOG7327N0.
PhylomeDBi Q64541.

Enzyme and pathway databases

SABIO-RK Q64541.

Miscellaneous databases

NextBioi 608083.

Gene expression databases

Genevestigatori Q64541.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 4 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed in testis."
    Shamraj O.I., Lingrel J.B.
    Proc. Natl. Acad. Sci. U.S.A. 91:12952-12956(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa."
    Jimenez T., Sanchez G., Wertheimer E., Blanco G.
    Reproduction 139:835-845(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAT1A4_RAT
AccessioniPrimary (citable) accession number: Q64541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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