Sodium/potassium-transporting ATPase subunit alpha-4

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Q64541 (AT1A4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-4
Short name=Na(+)/K(+) ATPase alpha-4 subunit
EC=3.6.3.9

Alternative name(s):
Sodium pump subunit alpha-4

Gene names

Name:	Atp1a4
Synonyms:	Atp1al2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1028 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility. Ref.2
Catalytic activity	ATP + H₂O + Na⁺(In) + K⁺(Out) = ADP + phosphate + Na⁺(Out) + K⁺(In).
Enzyme regulation	Specifically inhibited by an endogenous cardiac glycoside, ouabain By similarity.
Subunit structure	COmposed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Sodium
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro fertilization Inferred from electronic annotation. Source: Compara regulation of cellular pH Inferred from sequence or structural similarity. Source: UniProtKB regulation of membrane potential Inferred from electronic annotation. Source: Compara sperm motility Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from expression pattern PubMed 16264093. Source: RGD
Cellular_component	sodium:potassium-exchanging ATPase complex Traceable author statement PubMed 12112599. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sodium:potassium-exchanging ATPase activity Inferred from direct assay PubMed 10555956. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1028

1028

Sodium/potassium-transporting ATPase subunit alpha-4

PRO_0000046305

Regions

Topological domain

1 – 92

Cytoplasmic Potential

Transmembrane

93 – 113

Helical; Potential

Topological domain

114 – 137

Extracellular Potential

Transmembrane

138 – 158

Helical; Potential

Topological domain

159 – 294

136

Cytoplasmic Potential

Transmembrane

295 – 314

Helical; Potential

Topological domain

315 – 326

Extracellular Potential

Transmembrane

327 – 344

Helical; Potential

Topological domain

345 – 777

433

Cytoplasmic Potential

Transmembrane

778 – 797

Helical; Potential

Topological domain

798 – 807

Extracellular Potential

Transmembrane

808 – 828

Helical; Potential

Topological domain

829 – 848

Cytoplasmic Potential

Transmembrane

849 – 871

Helical; Potential

Topological domain

872 – 923

Extracellular Potential

Transmembrane

924 – 943

Helical; Potential

Topological domain

944 – 956

Cytoplasmic Potential

Transmembrane

957 – 975

Helical; Potential

Topological domain

976 – 990

Extracellular Potential

Transmembrane

991 – 1011

Helical; Potential

Topological domain

1012 – 1028

Cytoplasmic Potential

Region

87 – 89

Interaction with phosphoinositide-3 kinase By similarity

Sites

Active site

382

4-aspartylphosphate intermediate By similarity

Metal binding

722

Magnesium By similarity

Metal binding

726

Magnesium By similarity

Amino acid modifications

Modified residue

948

Phosphoserine; by PKA By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q64541 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 858FE008735D06FA
Show »

FASTA

1,028

114,005

        10         20         30         40         50         60 
MEPGKETAAT SEQKPRPTLR ASNTNRQPKV KRRKKDLEEL KKEVVMDDHK LTLDELSAKY 

        70         80         90        100        110        120 
SVDLTKGLSV TDAQEILTLN GPNVLTPPPT TPEWIKFCKQ LFGGFSLLLW TGSLLCFLAY 

       130        140        150        160        170        180 
GIHVSYYQEN ANKDNLYLGI VLSAVVIITG CFSYYQEAKS SKIMESFKTM VPQQALVIRD 

       190        200        210        220        230        240 
GEKMQINVRD VVLGDLVEVK GGDQVPADIR VIAAQGCKVD NSSLTGESEP QSRCPDCTHE 

       250        260        270        280        290        300 
NPLETRNIIF FSTNCVEGTA RGVVIATGDH TVMGRIASLT SGLTMGKTPI ATEIEHFIHI 

       310        320        330        340        350        360 
ITAVAVFLGV TFFFLSLILG YTWLDAVIFL IGIIVANVPE GLLATVTVCL TLTAKRMARK 

       370        380        390        400        410        420 
NCLVKNLEAV ETLGSTSTIC SDKTGTLTQN RMTVAHLWFD KTVYEADTSE EQTTGKTFPK 

       430        440        450        460        470        480 
SSDTWFYLAR IAGLCNRADF KPHQESLPIT KRTTTGDASE SALLKFIEQS YSPVSEMRQK 

       490        500        510        520        530        540 
NPKVAEIPFN STNKYQMSIH LLEDNSEAHV LLMKGAPERI LDFCSSFLLN GQEYPMDEEM 

       550        560        570        580        590        600 
KTDFQNAYIE LGGLGERVLG FCFLNLPSNF SKGFQFNTEE LNFPMENLCF AGLISMIDPP 

       610        620        630        640        650        660 
RTAVPDAVSK CRSAGIKVIM VTGDHPITAK AIAKSVGIIS EANETAEDIA ARLNISISQV 

       670        680        690        700        710        720 
SNKSIKAIVV HGSELKDMDS GQLDNILKSY KEIVFARTSP QQKLIIVEGC QRLGAIVAVT 

       730        740        750        760        770        780 
GDGVNDSPAL KKADIGIAMG ITGSDVSKQA ADMILLDDNF ASIVTGVEEG RLIFDNLKKS 

       790        800        810        820        830        840 
IAYTLTSNIP EITPFLLFIV LSIPLPLGTI TILCIDLGTD MVPAISLAYE TPESDIMKRL 

       850        860        870        880        890        900 
PRNPKTDNLV NDRLIGMAYG QIGMIQALAG FFTYFVILAE NGFKPLDLLG IRLYWDDTNL 

       910        920        930        940        950        960 
NDLEDTYGQQ WTYEQRKVVE FTCQTAFFIS IVIVQWADLI ICKTRRNSLF KQGMKNKVLI 

       970        980        990       1000       1010       1020 
FGLLEETILA ACLSYIPGMD VALRMYPLKI NWWFCALPYS VLIFIYDEVR KLIIRRRPGG 


WLEKETYY

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References

[1]	"A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed in testis." Shamraj O.I., Lingrel J.B. Proc. Natl. Acad. Sci. U.S.A. 91:12952-12956(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Testis.
[2]	"Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa." Jimenez T., Sanchez G., Wertheimer E., Blanco G. Reproduction 139:835-845(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15176 mRNA. Translation: AAB81285.1.
IPI	IPI00390795.
RefSeq	NP_001257959.1. NM_001271030.1. NP_074039.1. NM_022848.3.
UniGene	Rn.224462.
3D structure databases
ProteinModelPortal	Q64541.
SMR	Q64541. Positions 32-1028.
ModBase	Search...
PTM databases
PhosphoSite	Q64541.
Proteomic databases
PaxDb	Q64541.
PRIDE	Q64541.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000064601; ENSRNOP00000062079; ENSRNOG00000032378.
GeneID	29132.
KEGG	rno:29132.
UCSC	RGD:61952. rat.
Organism-specific databases
CTD	480.
RGD	61952. Atp1a4.
Phylogenomic databases
eggNOG	COG0474.
GeneTree	ENSGT00560000076866.
HOVERGEN	HBG004298.
KO	K01539.
OrthoDB	EOG4WDDB0.
Enzyme and pathway databases
SABIO-RK	Q64541.
Gene expression databases
ArrayExpress	Q64541.
Genevestigator	Q64541.
Family and domain databases
Gene3D	1.20.1110.10. 2 hits. 2.70.150.10. 2 hits. 3.40.1110.10. 1 hit.
InterPro	IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023299. ATPase_P-typ_cyto_domN. IPR005775. ATPase_P-typ_Na/K_IIC. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR008250. ATPase_P-typ_transduc_dom_A. IPR001757. Cation_transp_P_typ_ATPase. IPR023214. HAD-like_dom. [Graphical view]
PANTHER	PTHR24093. PTHR24093. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL2485.
NextBio	608083.

Entry information

Entry name

AT1A4_RAT

Accession

Primary (citable) accession number: Q64541

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents