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Q64541

- AT1A4_RAT

UniProt

Q64541 - AT1A4_RAT

Protein

Sodium/potassium-transporting ATPase subunit alpha-4

Gene

Atp1a4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility.1 Publication

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Enzyme regulationi

    Specifically inhibited by an endogenous cardiac glycoside, ouabain.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei382 – 38214-aspartylphosphate intermediateBy similarity
    Metal bindingi722 – 7221MagnesiumBy similarity
    Metal bindingi726 – 7261MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sodium:potassium-exchanging ATPase activity Source: MGI

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro
    2. fertilization Source: Ensembl
    3. potassium ion transport Source: RGD
    4. regulation of cellular pH Source: UniProtKB
    5. regulation of membrane potential Source: Ensembl
    6. sodium ion transmembrane transport Source: GOC
    7. sodium ion transport Source: RGD
    8. spermatogenesis Source: RGD
    9. sperm motility Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Enzyme and pathway databases

    SABIO-RKQ64541.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha-4 (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha-4 subunit
    Alternative name(s):
    Sodium pump subunit alpha-4
    Gene namesi
    Name:Atp1a4
    Synonyms:Atp1al2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi61952. Atp1a4.

    Subcellular locationi

    GO - Cellular componenti

    1. sodium:potassium-exchanging ATPase complex Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10281028Sodium/potassium-transporting ATPase subunit alpha-4PRO_0000046305Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei948 – 9481Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ64541.
    PRIDEiQ64541.

    PTM databases

    PhosphoSiteiQ64541.

    Expressioni

    Gene expression databases

    GenevestigatoriQ64541.

    Interactioni

    Subunit structurei

    COmposed of three subunits: alpha (catalytic), beta and gamma.

    Protein-protein interaction databases

    MINTiMINT-4997397.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64541.
    SMRiQ64541. Positions 32-1028.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9292CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini114 – 13724ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini159 – 294136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini315 – 32612ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini345 – 777433CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini798 – 80710ExtracellularSequence Analysis
    Topological domaini829 – 84820CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini872 – 92352ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini944 – 95613CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini976 – 99015ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1012 – 102817CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei93 – 11321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei138 – 15821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei295 – 31420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei327 – 34418HelicalSequence AnalysisAdd
    BLAST
    Transmembranei778 – 79720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei808 – 82821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei849 – 87123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei924 – 94320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei957 – 97519HelicalSequence AnalysisAdd
    BLAST
    Transmembranei991 – 101121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 893Interaction with phosphoinositide-3 kinaseBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00560000076866.
    HOVERGENiHBG004298.
    KOiK01539.
    OMAiKSSDTWF.
    OrthoDBiEOG7327N0.
    PhylomeDBiQ64541.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 4 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64541-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPGKETAAT SEQKPRPTLR ASNTNRQPKV KRRKKDLEEL KKEVVMDDHK     50
    LTLDELSAKY SVDLTKGLSV TDAQEILTLN GPNVLTPPPT TPEWIKFCKQ 100
    LFGGFSLLLW TGSLLCFLAY GIHVSYYQEN ANKDNLYLGI VLSAVVIITG 150
    CFSYYQEAKS SKIMESFKTM VPQQALVIRD GEKMQINVRD VVLGDLVEVK 200
    GGDQVPADIR VIAAQGCKVD NSSLTGESEP QSRCPDCTHE NPLETRNIIF 250
    FSTNCVEGTA RGVVIATGDH TVMGRIASLT SGLTMGKTPI ATEIEHFIHI 300
    ITAVAVFLGV TFFFLSLILG YTWLDAVIFL IGIIVANVPE GLLATVTVCL 350
    TLTAKRMARK NCLVKNLEAV ETLGSTSTIC SDKTGTLTQN RMTVAHLWFD 400
    KTVYEADTSE EQTTGKTFPK SSDTWFYLAR IAGLCNRADF KPHQESLPIT 450
    KRTTTGDASE SALLKFIEQS YSPVSEMRQK NPKVAEIPFN STNKYQMSIH 500
    LLEDNSEAHV LLMKGAPERI LDFCSSFLLN GQEYPMDEEM KTDFQNAYIE 550
    LGGLGERVLG FCFLNLPSNF SKGFQFNTEE LNFPMENLCF AGLISMIDPP 600
    RTAVPDAVSK CRSAGIKVIM VTGDHPITAK AIAKSVGIIS EANETAEDIA 650
    ARLNISISQV SNKSIKAIVV HGSELKDMDS GQLDNILKSY KEIVFARTSP 700
    QQKLIIVEGC QRLGAIVAVT GDGVNDSPAL KKADIGIAMG ITGSDVSKQA 750
    ADMILLDDNF ASIVTGVEEG RLIFDNLKKS IAYTLTSNIP EITPFLLFIV 800
    LSIPLPLGTI TILCIDLGTD MVPAISLAYE TPESDIMKRL PRNPKTDNLV 850
    NDRLIGMAYG QIGMIQALAG FFTYFVILAE NGFKPLDLLG IRLYWDDTNL 900
    NDLEDTYGQQ WTYEQRKVVE FTCQTAFFIS IVIVQWADLI ICKTRRNSLF 950
    KQGMKNKVLI FGLLEETILA ACLSYIPGMD VALRMYPLKI NWWFCALPYS 1000
    VLIFIYDEVR KLIIRRRPGG WLEKETYY 1028
    Length:1,028
    Mass (Da):114,005
    Last modified:November 1, 1996 - v1
    Checksum:i858FE008735D06FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15176 mRNA. Translation: AAB81285.1.
    RefSeqiNP_001257959.1. NM_001271030.1.
    NP_074039.1. NM_022848.3.
    UniGeneiRn.224462.

    Genome annotation databases

    EnsembliENSRNOT00000064601; ENSRNOP00000062079; ENSRNOG00000032378.
    GeneIDi29132.
    KEGGirno:29132.
    UCSCiRGD:61952. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15176 mRNA. Translation: AAB81285.1 .
    RefSeqi NP_001257959.1. NM_001271030.1.
    NP_074039.1. NM_022848.3.
    UniGenei Rn.224462.

    3D structure databases

    ProteinModelPortali Q64541.
    SMRi Q64541. Positions 32-1028.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4997397.

    PTM databases

    PhosphoSitei Q64541.

    Proteomic databases

    PaxDbi Q64541.
    PRIDEi Q64541.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000064601 ; ENSRNOP00000062079 ; ENSRNOG00000032378 .
    GeneIDi 29132.
    KEGGi rno:29132.
    UCSCi RGD:61952. rat.

    Organism-specific databases

    CTDi 480.
    RGDi 61952. Atp1a4.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00560000076866.
    HOVERGENi HBG004298.
    KOi K01539.
    OMAi KSSDTWF.
    OrthoDBi EOG7327N0.
    PhylomeDBi Q64541.

    Enzyme and pathway databases

    SABIO-RK Q64541.

    Miscellaneous databases

    NextBioi 608083.

    Gene expression databases

    Genevestigatori Q64541.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 4 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed in testis."
      Shamraj O.I., Lingrel J.B.
      Proc. Natl. Acad. Sci. U.S.A. 91:12952-12956(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. "Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa."
      Jimenez T., Sanchez G., Wertheimer E., Blanco G.
      Reproduction 139:835-845(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiAT1A4_RAT
    AccessioniPrimary (citable) accession number: Q64541
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3