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Q64536 (PDK2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial
EC=2.7.11.2
Alternative name(s):
PDK P45
Pyruvate dehydrogenase kinase isoform 2
Gene names
Name:Pdk2
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Catalytic activity

ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.

Subcellular location

Mitochondrion matrix.

Tissue specificity

Highest level of expression in heart and skeletal muscle and the lowest in spleen and lung. Liver, kidney, brain and testis levels are intermediate.

Sequence similarities

Belongs to the PDK/BCKDK protein kinase family.

Contains 1 histidine kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 407[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrialPRO_0000023442

Regions

Domain135 – 364230Histidine kinase
Nucleotide binding251 – 2588ATP By similarity
Nucleotide binding309 – 3102ATP By similarity
Nucleotide binding325 – 3306ATP By similarity

Sites

Binding site2901ATP By similarity

Secondary structure

1 ............................................... 407
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q64536 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A01C377290A69EF4

FASTA40746,106
        10         20         30         40         50         60 
MRWFRALLKN ASLAGAPKYI EHFSKFSPSP LSMKQFLDFG SSNACEKTSF TFLRQELPVR 

        70         80         90        100        110        120 
LANIMKEINL LPDRVLSTPS VQLVQSWYVQ SLLDIMEFLD KDPEDHRTLS QFTDALVTIR 

       130        140        150        160        170        180 
NRHNDVVPTM AQGVLEYKDT YGDDPVSNQN IQYFLDRFYL SRISIRMLIN QHTLIFDGST 

       190        200        210        220        230        240 
NPAHPKHIGS IDPNCSVSDV VKDAYDMAKL LCDKYYMASP DLEIQEVNAT NATQPIHMVY 

       250        260        270        280        290        300 
VPSHLYHMLF ELFKNAMRAT VESHESSLTL PPIKIMVALG EEDLSIKMSD RGGGVPLRKI 

       310        320        330        340        350        360 
ERLFSYMYST APTPQPGTGG TPLAGFGYGL PISRLYAKYF QGDLQLFSME GFGTDAVIYL 

       370        380        390        400 
KALSTDSVER LPVYNKSAWR HYQTIQEAGD WCVPSTEPKN TSTYRVS

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the p45 subunit of pyruvate dehydrogenase kinase."
Popov K.M., Kedishvili N.Y., Zhao Y., Gudi R., Harris R.A.
J. Biol. Chem. 269:29720-29724(1994) [PubMed: 7961963] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U10357 mRNA. Translation: AAB54084.1.
BC061823 mRNA. Translation: AAH61823.1.
IPIIPI00327834.
PIRA55305.
RefSeqNP_110499.1. NM_030872.1.
UniGeneRn.88597.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JM6X-ray2.50A/B1-407[»]
3CRKX-ray2.30A/B1-407[»]
3CRLX-ray2.61A/B1-407[»]
ProteinModelPortalQ64536.
SMRQ64536. Positions 12-404.
ModBaseSearch...

Protein-protein interaction databases

IntActQ64536. 1 interaction.
STRINGQ64536.

Proteomic databases

PRIDEQ64536.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID81530.
KEGGrno:81530.

Organism-specific databases

CTD5164.
RGD69428. Pdk2.

Phylogenomic databases

eggNOGmaNOG18250.
HOVERGENHBG000511.
InParanoidQ64536.
OrthoDBEOG4G1MGF.
PhylomeDBQ64536.

Gene expression databases

ArrayExpressQ64536.
GenevestigatorQ64536.
GermOnlineENSRNOG00000004172. Rattus norvegicus.

Family and domain databases

InterProIPR003594. ATPase-like_ATP-bd.
IPR018955. BCDHK/PDK_N.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
G3DSA:1.20.140.20. BCDHK/PDK_N. 1 hit.
KOK00898.
PfamPF10436. BCDHK_Adom3. 1 hit.
PF02518. HATPase_c. 1 hit.
[Graphical view]
SMARTSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF69012. BCDHK/PDK_N. 1 hit.
PROSITEPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615077.

Entry information

Entry namePDK2_RAT
AccessionPrimary (citable) accession number: Q64536
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 16, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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