Q64527 (WNT8A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-8a

Alternative name(s):
Protein Wnt-8d
Stimulated by retinoic acid gene 11 protein

Gene names

Name:	Wnt8a
Synonyms:	Stra11, Wnt8d

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Developmental stage	Expression in early stages of embryogenesis. Expression begins in the posterior region of early primitive streak-stage embryos and after it spreads into the embryonic ectoderm up to a sharp rostral boundary at the base of the developing headfolds. Expressed transiently in the newly formed mesoderm. Expression is down-regulated during somitogenesis. The expression is highly restricted during gastrulation and neurulation, both temporally and spatially.
Induction	By retinoic acid.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain segmentation Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway involved in cardiac muscle cell fate commitment Inferred from electronic annotation. Source: Compara cell migration involved in gastrulation Inferred from Biological aspect of Ancestor. Source: RefGenome cell morphogenesis Inferred from mutant phenotype PubMed 16256739. Source: MGI dorsal/ventral pattern formation Inferred from Biological aspect of Ancestor. Source: RefGenome ectoderm development Inferred from Biological aspect of Ancestor. Source: RefGenome endoderm development Inferred from mutant phenotype PubMed 16256739. Source: MGI establishment of organ orientation Inferred from mutant phenotype PubMed 16256739. Source: MGI eye development Inferred from Biological aspect of Ancestor. Source: RefGenome hindbrain development Inferred from Biological aspect of Ancestor. Source: RefGenome mesodermal cell fate commitment Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome neural plate pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome notochord development Inferred from Biological aspect of Ancestor. Source: RefGenome palate development Inferred from electronic annotation. Source: Compara polarity specification of anterior/posterior axis Inferred from mutant phenotype PubMed 16256739. Source: MGI polarity specification of proximal/distal axis Inferred from mutant phenotype PubMed 16256739. Source: MGI positive regulation of fibroblast growth factor receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of protein localization Inferred from mutant phenotype PubMed 16256739. Source: MGI somitogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome spinal cord anterior/posterior patterning Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 354	335	Protein Wnt-8a		PRO_0000041449
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	32	1	R → G in CAA93117. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q64527 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: A91D5245F5B6FE65	FASTA	354	39,573
	10 20 30 40 50 60 MGHLLMLWVA AGMCYPALGA SAWSVNNFLI TRPKAYLTYT ASVALGAQIG IEECKFQFAW 70 80 90 100 110 120 ERWNCPEHAF QFSTHNRLRA ATRETSFIHA IRSAAIMYAV TKNCSMGDLE NCGCDESQNG 130 140 150 160 170 180 KTGGHGWIWG GCSDNVEFGE KISRLFVDSL EKGKDARALV NLHNNRAGRL AVRASTKRTC 190 200 210 220 230 240 KCHGISGSCS IQTCWLQLAD FRQMGNYLKA KYDRALKIEM DKRQLRAGNR AEGRWALTEA 250 260 270 280 290 300 FLPSTEAELI FLEGSPDYCN RNASLSIQGT EGRECLQNAR SASRREQRSC GRLCTECGLQ 310 320 330 340 350 VEERRAEAVS SCDCNFQWCC TVKCGQCRRV VSRYYCTRPV GSARPRGRGK DSAW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new mouse member of the Wnt gene family, mWnt-8, is expressed during early embryogenesis and is ectopically induced by retinoic acid." Bouillet P., Oulad-Abdelghani M., Ward S.J., Bronner S., Chambon P., Dolle P. Mech. Dev. 58:141-152(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68889 mRNA. Translation: CAA93117.1. AC074335 Genomic DNA. No translation available.
IPI	IPI00135504.
RefSeq	NP_033316.1. NM_009290.2.
UniGene	Mm.558.
3D structure databases
ProteinModelPortal	Q64527.
ModBase	Search...
PTM databases
PhosphoSite	Q64527.
Proteomic databases
PRIDE	Q64527.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000012426; ENSMUSP00000012426; ENSMUSG00000012282.
GeneID	20890.
KEGG	mmu:20890.
Organism-specific databases
CTD	7478.
MGI	MGI:107924. Wnt8a.
Phylogenomic databases
eggNOG	NOG277037.
GeneTree	ENSGT00690000101857.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	Q64527.
KO	K00714.
OMA	RALKIEM.
OrthoDB	EOG4DFPNP.
Gene expression databases
Bgee	Q64527.
CleanEx	MM_WNT8A.
Genevestigator	Q64527.
GermOnline	ENSMUSG00000012282. Mus musculus.
Family and domain databases
InterPro	IPR005817. Wnt. IPR013301. Wnt8. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF6. PTHR12027:SF6. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01892. WNT8PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	299745.
SOURCE	Search...

Entry information

Entry name

WNT8A_MOUSE

Accession

Primary (citable) accession number: Q64527
Secondary accession number(s): E9QL90

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program