Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Wnt-8a

Gene

Wnt8a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).By similarity

GO - Molecular functioni

  1. frizzled binding Source: GO_Central

GO - Biological processi

  1. cell fate commitment Source: GO_Central
  2. cell morphogenesis Source: MGI
  3. endoderm development Source: MGI
  4. establishment of organ orientation Source: MGI
  5. neuron differentiation Source: GO_Central
  6. palate development Source: MGI
  7. polarity specification of anterior/posterior axis Source: MGI
  8. polarity specification of proximal/distal axis Source: MGI
  9. regionalization Source: MGI
  10. regulation of protein localization Source: MGI
  11. Wnt signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_295052. WNT ligand biogenesis and trafficking.
REACT_317277. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_335672. TCF dependent signaling in response to WNT.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-8a
Alternative name(s):
Protein Wnt-8d
Stimulated by retinoic acid gene 11 protein
Gene namesi
Name:Wnt8a
Synonyms:Stra11, Wnt8d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:107924. Wnt8a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: GO_Central
  2. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 354335Protein Wnt-8aPRO_0000041449Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 65By similarity
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi104 ↔ 112By similarity
Disulfide bondi114 ↔ 132By similarity
Disulfide bondi180 ↔ 194By similarity
Disulfide bondi182 ↔ 189By similarity
Lipidationi186 – 1861O-palmitoleyl serineBy similarity
Disulfide bondi259 ↔ 297By similarity
Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi275 ↔ 290By similarity
Disulfide bondi294 ↔ 336By similarity
Disulfide bondi312 ↔ 327By similarity
Disulfide bondi314 ↔ 324By similarity
Disulfide bondi319 ↔ 320By similarity

Post-translational modificationi

Palmitoleylation is required for efficient binding to frizzled receptors (By similarity). Depalmitoleylation leads to Wnt signaling pathway inhibition (By similarity).By similarity
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT8A.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PRIDEiQ64527.

PTM databases

PhosphoSiteiQ64527.

Expressioni

Developmental stagei

Expression in early stages of embryogenesis. Expression begins in the posterior region of early primitive streak-stage embryos and after it spreads into the embryonic ectoderm up to a sharp rostral boundary at the base of the developing headfolds. Expressed transiently in the newly formed mesoderm. Expression is down-regulated during somitogenesis. The expression is highly restricted during gastrulation and neurulation, both temporally and spatially.

Inductioni

By retinoic acid.

Gene expression databases

BgeeiQ64527.
CleanExiMM_WNT8A.
GenevestigatoriQ64527.

Interactioni

Protein-protein interaction databases

BioGridi203552. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG277037.
GeneTreeiENSGT00690000101857.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiQ64527.
KOiK00714.
OMAiCCTVRCD.
OrthoDBiEOG7C8GJ8.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF92. PTHR12027:SF92. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHLLMLWVA AGMCYPALGA SAWSVNNFLI TRPKAYLTYT ASVALGAQIG
60 70 80 90 100
IEECKFQFAW ERWNCPEHAF QFSTHNRLRA ATRETSFIHA IRSAAIMYAV
110 120 130 140 150
TKNCSMGDLE NCGCDESQNG KTGGHGWIWG GCSDNVEFGE KISRLFVDSL
160 170 180 190 200
EKGKDARALV NLHNNRAGRL AVRASTKRTC KCHGISGSCS IQTCWLQLAD
210 220 230 240 250
FRQMGNYLKA KYDRALKIEM DKRQLRAGNR AEGRWALTEA FLPSTEAELI
260 270 280 290 300
FLEGSPDYCN RNASLSIQGT EGRECLQNAR SASRREQRSC GRLCTECGLQ
310 320 330 340 350
VEERRAEAVS SCDCNFQWCC TVKCGQCRRV VSRYYCTRPV GSARPRGRGK

DSAW
Length:354
Mass (Da):39,573
Last modified:July 27, 2011 - v2
Checksum:iA91D5245F5B6FE65
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321R → G in CAA93117 (PubMed:8887323).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68889 mRNA. Translation: CAA93117.1.
AC074335 Genomic DNA. No translation available.
CCDSiCCDS29129.1.
RefSeqiNP_033316.1. NM_009290.2.
XP_006525801.1. XM_006525738.2.
UniGeneiMm.558.

Genome annotation databases

EnsembliENSMUST00000012426; ENSMUSP00000012426; ENSMUSG00000012282.
GeneIDi20890.
KEGGimmu:20890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68889 mRNA. Translation: CAA93117.1.
AC074335 Genomic DNA. No translation available.
CCDSiCCDS29129.1.
RefSeqiNP_033316.1. NM_009290.2.
XP_006525801.1. XM_006525738.2.
UniGeneiMm.558.

3D structure databases

ProteinModelPortaliQ64527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203552. 1 interaction.

PTM databases

PhosphoSiteiQ64527.

Proteomic databases

PRIDEiQ64527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000012426; ENSMUSP00000012426; ENSMUSG00000012282.
GeneIDi20890.
KEGGimmu:20890.

Organism-specific databases

CTDi7478.
MGIiMGI:107924. Wnt8a.

Phylogenomic databases

eggNOGiNOG277037.
GeneTreeiENSGT00690000101857.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiQ64527.
KOiK00714.
OMAiCCTVRCD.
OrthoDBiEOG7C8GJ8.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiREACT_295052. WNT ligand biogenesis and trafficking.
REACT_317277. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_335672. TCF dependent signaling in response to WNT.

Miscellaneous databases

NextBioi299745.
PROiQ64527.
SOURCEiSearch...

Gene expression databases

BgeeiQ64527.
CleanExiMM_WNT8A.
GenevestigatoriQ64527.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF92. PTHR12027:SF92. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new mouse member of the Wnt gene family, mWnt-8, is expressed during early embryogenesis and is ectopically induced by retinoic acid."
    Bouillet P., Oulad-Abdelghani M., Ward S.J., Bronner S., Chambon P., Dolle P.
    Mech. Dev. 58:141-152(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiWNT8A_MOUSE
AccessioniPrimary (citable) accession number: Q64527
Secondary accession number(s): E9QL90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: April 29, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.