Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q64521

- GPDM_MOUSE

UniProt

Q64521 - GPDM_MOUSE

Protein

Glycerol-3-phosphate dehydrogenase, mitochondrial

Gene

Gpd2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

    Cofactori

    FAD.

    Enzyme regulationi

    Calcium-binding enhance the activity of the enzyme.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi71 – 9929FADSequence AnalysisAdd
    BLAST
    Calcium bindingi672 – 68312PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. camera-type eye development Source: MGI
    2. gluconeogenesis Source: MGI
    3. glycerol-3-phosphate metabolic process Source: MGI
    4. glycerol catabolic process Source: UniProtKB-UniPathway
    5. multicellular organism growth Source: MGI

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calcium, FAD, Flavoprotein, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00618; UER00673.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase, mitochondrial (EC:1.1.5.3)
    Short name:
    GPD-M
    Short name:
    GPDH-M
    Alternative name(s):
    Protein TISP38
    Gene namesi
    Name:Gpd2
    Synonyms:Gdm1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:99778. Gpd2.

    Subcellular locationi

    GO - Cellular componenti

    1. glycerol-3-phosphate dehydrogenase complex Source: MGI
    2. mitochondrial inner membrane Source: MGI
    3. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4242MitochondrionBy similarityAdd
    BLAST
    Chaini43 – 727685Glycerol-3-phosphate dehydrogenase, mitochondrialPRO_0000010430Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei601 – 6011Phosphotyrosine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ64521.
    PaxDbiQ64521.
    PRIDEiQ64521.

    2D gel databases

    REPRODUCTION-2DPAGEQ64521.

    PTM databases

    PhosphoSiteiQ64521.

    Expressioni

    Gene expression databases

    ArrayExpressiQ64521.
    BgeeiQ64521.
    CleanExiMM_GPD2.
    GenevestigatoriQ64521.

    Interactioni

    Protein-protein interaction databases

    IntActiQ64521. 4 interactions.
    MINTiMINT-4119960.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64521.
    SMRiQ64521. Positions 68-597, 628-689.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini623 – 65836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini659 – 69436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0578.
    GeneTreeiENSGT00390000001718.
    HOVERGENiHBG005897.
    KOiK00111.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR006076. FAD-dep_OxRdtase.
    IPR000447. G3P_DH_FAD-dep.
    [Graphical view]
    PfamiPF01266. DAO. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    PRINTSiPR01001. FADG3PDH.
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS00977. FAD_G3PDH_1. 1 hit.
    PS00978. FAD_G3PDH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q64521-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFQKAVKGT ILVGGGALAT VLGLSPFAHY RRKQVSLAYV EAAGYLTEPV    50
    NREPPSREAQ LMTLKNTPEF DILVIGGGAT GCGCALDAVT RGLKTALVER 100
    DDFSSGTSSR STKLIHGGVR YLQKAIMNLD VEQYRMVKEA LHERANLLEI 150
    APHLSAPLPI MLPLYKWWQL PYYWVGIKMY DLVAGSQCLK SSYVLSKSRA 200
    LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG AATANYMEVV 250
    SLLKKTDPET GKERVSGARC KDVLTGQEFD VRAKCVINAS GPFTDSVRKM 300
    DDKNVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT 350
    IAGTTDTPTD VTHHPIPSEE DINFILNEVR NYLSSDVEVR RGDVLAAWSG 400
    IRPLVTDPKS ADTQSISRNH VVDISDSGLI TIAGGKWTTY RSMAEDTVDA 450
    AVKFHNLNAG PSRTVGLFLQ GGKDWSPTLY IRLVQDYGLE SEVAQHLAKT 500
    YGDKAFEVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG IKEYACTAVD 550
    MISRRTRLAF LNVQAAEEAL PRIVELMGRE LNWSELRKQE ELETATRFLY 600
    YEMGYKSRTE QLTDSTEISL LPSDIDRYKK RFHKFDEDEK GFITIVDVQR 650
    VLESINVQMD ENTLHEILCE VDLNKNGQVE LHEFLQLMSA VQKGRVSGSR 700
    LAILMKTAEE NLDRRVPIPV DRSCGGL 727
    Length:727
    Mass (Da):80,954
    Last modified:May 1, 2007 - v2
    Checksum:i319F52E31CFDFC44
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261P → Q in AAB50545. (PubMed:8951039)Curated
    Sequence conflicti26 – 261P → Q in AAH21359. (PubMed:15489334)Curated
    Sequence conflicti42 – 421Missing in BAC28685. (PubMed:16141072)Curated
    Sequence conflicti158 – 1581L → V in AAB50545. (PubMed:8951039)Curated
    Sequence conflicti295 – 2951D → E in BAA08926. (PubMed:8772729)Curated
    Sequence conflicti449 – 4491D → N in AAH21359. (PubMed:15489334)Curated
    Sequence conflicti498 – 4981A → P in BAA08926. (PubMed:8772729)Curated
    Sequence conflicti582 – 5821N → D in AAB50545. (PubMed:8951039)Curated
    Sequence conflicti593 – 5931E → G in AAB50545. (PubMed:8951039)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60987 mRNA. Translation: AAB50545.1.
    D50430 mRNA. Translation: BAA08926.1.
    AK034353 mRNA. Translation: BAC28685.1.
    AK144716 mRNA. Translation: BAE26028.1.
    AK149851 mRNA. Translation: BAE29123.1.
    AK167152 mRNA. Translation: BAE39294.1.
    BC021359 mRNA. Translation: AAH21359.1.
    AH009802 Genomic DNA. Translation: AAG12342.1.
    AB045714 mRNA. Translation: BAB97201.1.
    CCDSiCCDS16045.1.
    RefSeqiNP_001139292.1. NM_001145820.1.
    NP_034404.3. NM_010274.3.
    UniGeneiMm.3711.

    Genome annotation databases

    EnsembliENSMUST00000028167; ENSMUSP00000028167; ENSMUSG00000026827.
    ENSMUST00000169687; ENSMUSP00000130992; ENSMUSG00000026827.
    GeneIDi14571.
    KEGGimmu:14571.
    UCSCiuc008jsc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60987 mRNA. Translation: AAB50545.1 .
    D50430 mRNA. Translation: BAA08926.1 .
    AK034353 mRNA. Translation: BAC28685.1 .
    AK144716 mRNA. Translation: BAE26028.1 .
    AK149851 mRNA. Translation: BAE29123.1 .
    AK167152 mRNA. Translation: BAE39294.1 .
    BC021359 mRNA. Translation: AAH21359.1 .
    AH009802 Genomic DNA. Translation: AAG12342.1 .
    AB045714 mRNA. Translation: BAB97201.1 .
    CCDSi CCDS16045.1.
    RefSeqi NP_001139292.1. NM_001145820.1.
    NP_034404.3. NM_010274.3.
    UniGenei Mm.3711.

    3D structure databases

    ProteinModelPortali Q64521.
    SMRi Q64521. Positions 68-597, 628-689.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q64521. 4 interactions.
    MINTi MINT-4119960.

    PTM databases

    PhosphoSitei Q64521.

    2D gel databases

    REPRODUCTION-2DPAGE Q64521.

    Proteomic databases

    MaxQBi Q64521.
    PaxDbi Q64521.
    PRIDEi Q64521.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028167 ; ENSMUSP00000028167 ; ENSMUSG00000026827 .
    ENSMUST00000169687 ; ENSMUSP00000130992 ; ENSMUSG00000026827 .
    GeneIDi 14571.
    KEGGi mmu:14571.
    UCSCi uc008jsc.2. mouse.

    Organism-specific databases

    CTDi 2820.
    MGIi MGI:99778. Gpd2.

    Phylogenomic databases

    eggNOGi COG0578.
    GeneTreei ENSGT00390000001718.
    HOVERGENi HBG005897.
    KOi K00111.

    Enzyme and pathway databases

    UniPathwayi UPA00618 ; UER00673 .

    Miscellaneous databases

    ChiTaRSi GPD2. mouse.
    NextBioi 286290.
    PROi Q64521.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64521.
    Bgeei Q64521.
    CleanExi MM_GPD2.
    Genevestigatori Q64521.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR006076. FAD-dep_OxRdtase.
    IPR000447. G3P_DH_FAD-dep.
    [Graphical view ]
    Pfami PF01266. DAO. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    PRINTSi PR01001. FADG3PDH.
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS00977. FAD_G3PDH_1. 1 hit.
    PS00978. FAD_G3PDH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and tissue-dependent RNA expression of mouse FAD-linked glycerol-3-phosphate dehydrogenase."
      Koza R.A., Kozak U.C., Brown L.J., Leiter E.H., Macdonald M.J., Kozak L.P.
      Arch. Biochem. Biophys. 336:97-104(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Adipocyte.
    2. "Effect of mitochondrial and/or cytosolic glycerol 3-phosphate dehydrogenase overexpression on glucose-stimulated insulin secretion from MIN6 and HIT cells."
      Ishihara H., Nakazaki M., Kanegae Y., Inukai K., Asano T., Katagiri H., Yazaki Y., Kikuchi M., Miyazaki J., Saito I., Oka Y.
      Diabetes 45:1238-1244(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Bone marrow, Diencephalon and Lung.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.
    5. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    6. "Genomic sequence of mouse mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH)."
      Weitzel J.M.
      Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 390-686.
      Strain: 129/Sv.
      Tissue: Liver.
    7. "Mus musculus TISP38 mRNA."
      Tamura K., Nishimune Y., Nojima H.
      Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 611-727.
      Tissue: Testis.
    8. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-601, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiGPDM_MOUSE
    AccessioniPrimary (citable) accession number: Q64521
    Secondary accession number(s): Q3TK51
    , Q3UDY8, Q61507, Q8CBX6, Q8K4U5, Q8VDT0, Q9ERP0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3