Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerol-3-phosphate dehydrogenase, mitochondrial

Gene

Gpd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

Enzyme regulationi

Calcium-binding enhance the activity of the enzyme.

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol-3-phosphate dehydrogenase, mitochondrial (Gpd2)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi71 – 99FADSequence analysisAdd BLAST29
Calcium bindingi672 – 683PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • camera-type eye development Source: MGI
  • gluconeogenesis Source: MGI
  • glycerol-3-phosphate metabolic process Source: MGI
  • glycerol catabolic process Source: UniProtKB-UniPathway
  • multicellular organism growth Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding

Enzyme and pathway databases

BRENDAi1.1.5.3. 3474.
ReactomeiR-MMU-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
UniPathwayiUPA00618; UER00673.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial (EC:1.1.5.3)
Short name:
GPD-M
Short name:
GPDH-M
Alternative name(s):
Protein TISP38
Gene namesi
Name:Gpd2
Synonyms:Gdm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:99778. Gpd2.

Subcellular locationi

GO - Cellular componenti

  • glycerol-3-phosphate dehydrogenase complex Source: MGI
  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionBy similarityAdd BLAST42
ChainiPRO_000001043043 – 727Glycerol-3-phosphate dehydrogenase, mitochondrialAdd BLAST685

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei601PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ64521.
MaxQBiQ64521.
PaxDbiQ64521.
PeptideAtlasiQ64521.
PRIDEiQ64521.

2D gel databases

REPRODUCTION-2DPAGEQ64521.

PTM databases

iPTMnetiQ64521.
PhosphoSitePlusiQ64521.
SwissPalmiQ64521.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026827.
CleanExiMM_GPD2.
ExpressionAtlasiQ64521. baseline and differential.
GenevisibleiQ64521. MM.

Interactioni

Protein-protein interaction databases

IntActiQ64521. 5 interactors.
MINTiMINT-4119960.
STRINGi10090.ENSMUSP00000028167.

Structurei

3D structure databases

ProteinModelPortaliQ64521.
SMRiQ64521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini623 – 658EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini659 – 694EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0042. Eukaryota.
COG0578. LUCA.
GeneTreeiENSGT00390000001718.
HOVERGENiHBG005897.
InParanoidiQ64521.
KOiK00111.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR031656. DAO_C.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16901. DAO_C. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFQKAVKGT ILVGGGALAT VLGLSPFAHY RRKQVSLAYV EAAGYLTEPV
60 70 80 90 100
NREPPSREAQ LMTLKNTPEF DILVIGGGAT GCGCALDAVT RGLKTALVER
110 120 130 140 150
DDFSSGTSSR STKLIHGGVR YLQKAIMNLD VEQYRMVKEA LHERANLLEI
160 170 180 190 200
APHLSAPLPI MLPLYKWWQL PYYWVGIKMY DLVAGSQCLK SSYVLSKSRA
210 220 230 240 250
LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG AATANYMEVV
260 270 280 290 300
SLLKKTDPET GKERVSGARC KDVLTGQEFD VRAKCVINAS GPFTDSVRKM
310 320 330 340 350
DDKNVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT
360 370 380 390 400
IAGTTDTPTD VTHHPIPSEE DINFILNEVR NYLSSDVEVR RGDVLAAWSG
410 420 430 440 450
IRPLVTDPKS ADTQSISRNH VVDISDSGLI TIAGGKWTTY RSMAEDTVDA
460 470 480 490 500
AVKFHNLNAG PSRTVGLFLQ GGKDWSPTLY IRLVQDYGLE SEVAQHLAKT
510 520 530 540 550
YGDKAFEVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG IKEYACTAVD
560 570 580 590 600
MISRRTRLAF LNVQAAEEAL PRIVELMGRE LNWSELRKQE ELETATRFLY
610 620 630 640 650
YEMGYKSRTE QLTDSTEISL LPSDIDRYKK RFHKFDEDEK GFITIVDVQR
660 670 680 690 700
VLESINVQMD ENTLHEILCE VDLNKNGQVE LHEFLQLMSA VQKGRVSGSR
710 720
LAILMKTAEE NLDRRVPIPV DRSCGGL
Length:727
Mass (Da):80,954
Last modified:May 1, 2007 - v2
Checksum:i319F52E31CFDFC44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26P → Q in AAB50545 (PubMed:8951039).Curated1
Sequence conflicti26P → Q in AAH21359 (PubMed:15489334).Curated1
Sequence conflicti42Missing in BAC28685 (PubMed:16141072).Curated1
Sequence conflicti158L → V in AAB50545 (PubMed:8951039).Curated1
Sequence conflicti295D → E in BAA08926 (PubMed:8772729).Curated1
Sequence conflicti449D → N in AAH21359 (PubMed:15489334).Curated1
Sequence conflicti498A → P in BAA08926 (PubMed:8772729).Curated1
Sequence conflicti582N → D in AAB50545 (PubMed:8951039).Curated1
Sequence conflicti593E → G in AAB50545 (PubMed:8951039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60987 mRNA. Translation: AAB50545.1.
D50430 mRNA. Translation: BAA08926.1.
AK034353 mRNA. Translation: BAC28685.1.
AK144716 mRNA. Translation: BAE26028.1.
AK149851 mRNA. Translation: BAE29123.1.
AK167152 mRNA. Translation: BAE39294.1.
BC021359 mRNA. Translation: AAH21359.1.
AH009802 Genomic DNA. Translation: AAG12342.1.
AB045714 mRNA. Translation: BAB97201.1.
CCDSiCCDS16045.1.
RefSeqiNP_001139292.1. NM_001145820.1.
NP_034404.3. NM_010274.3.
UniGeneiMm.3711.

Genome annotation databases

EnsembliENSMUST00000028167; ENSMUSP00000028167; ENSMUSG00000026827.
ENSMUST00000169687; ENSMUSP00000130992; ENSMUSG00000026827.
GeneIDi14571.
KEGGimmu:14571.
UCSCiuc008jsc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60987 mRNA. Translation: AAB50545.1.
D50430 mRNA. Translation: BAA08926.1.
AK034353 mRNA. Translation: BAC28685.1.
AK144716 mRNA. Translation: BAE26028.1.
AK149851 mRNA. Translation: BAE29123.1.
AK167152 mRNA. Translation: BAE39294.1.
BC021359 mRNA. Translation: AAH21359.1.
AH009802 Genomic DNA. Translation: AAG12342.1.
AB045714 mRNA. Translation: BAB97201.1.
CCDSiCCDS16045.1.
RefSeqiNP_001139292.1. NM_001145820.1.
NP_034404.3. NM_010274.3.
UniGeneiMm.3711.

3D structure databases

ProteinModelPortaliQ64521.
SMRiQ64521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64521. 5 interactors.
MINTiMINT-4119960.
STRINGi10090.ENSMUSP00000028167.

PTM databases

iPTMnetiQ64521.
PhosphoSitePlusiQ64521.
SwissPalmiQ64521.

2D gel databases

REPRODUCTION-2DPAGEQ64521.

Proteomic databases

EPDiQ64521.
MaxQBiQ64521.
PaxDbiQ64521.
PeptideAtlasiQ64521.
PRIDEiQ64521.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028167; ENSMUSP00000028167; ENSMUSG00000026827.
ENSMUST00000169687; ENSMUSP00000130992; ENSMUSG00000026827.
GeneIDi14571.
KEGGimmu:14571.
UCSCiuc008jsc.2. mouse.

Organism-specific databases

CTDi2820.
MGIiMGI:99778. Gpd2.

Phylogenomic databases

eggNOGiKOG0042. Eukaryota.
COG0578. LUCA.
GeneTreeiENSGT00390000001718.
HOVERGENiHBG005897.
InParanoidiQ64521.
KOiK00111.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00673.
BRENDAi1.1.5.3. 3474.
ReactomeiR-MMU-535734. Fatty acid, triacylglycerol, and ketone body metabolism.

Miscellaneous databases

ChiTaRSiGpd2. mouse.
PROiQ64521.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026827.
CleanExiMM_GPD2.
ExpressionAtlasiQ64521. baseline and differential.
GenevisibleiQ64521. MM.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR031656. DAO_C.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16901. DAO_C. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPDM_MOUSE
AccessioniPrimary (citable) accession number: Q64521
Secondary accession number(s): Q3TK51
, Q3UDY8, Q61507, Q8CBX6, Q8K4U5, Q8VDT0, Q9ERP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.