Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanylate kinase

Gene

Guk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for recycling GMP and indirectly, cGMP.

Catalytic activityi

ATP + GMP = ADP + GDP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei441 Publication1
Active sitei1371 Publication1
Active sitei1481 Publication1
Binding sitei171ATP1
Binding sitei172ATP; via carbonyl oxygen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18ATP8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • guanylate kinase activity Source: UniProtKB

GO - Biological processi

  • purine nucleotide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.4.8. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate kinase (EC:2.7.4.8)
Alternative name(s):
GMP kinase
Gene namesi
Name:Guk1
Synonyms:Gmk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:95871. Guk1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001706522 – 198Guanylate kinaseAdd BLAST197

Proteomic databases

EPDiQ64520.
MaxQBiQ64520.
PaxDbiQ64520.
PRIDEiQ64520.

PTM databases

iPTMnetiQ64520.
PhosphoSitePlusiQ64520.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020444.
CleanExiMM_GUK1.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ64520. 2 interactors.
MINTiMINT-4099756.
STRINGi10090.ENSMUSP00000127566.

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Helixi17 – 28Combined sources12
Turni29 – 31Combined sources3
Beta strandi32 – 34Combined sources3
Turni49 – 51Combined sources3
Helixi58 – 67Combined sources10
Beta strandi70 – 76Combined sources7
Beta strandi79 – 84Combined sources6
Helixi85 – 93Combined sources9
Beta strandi97 – 101Combined sources5
Helixi104 – 110Combined sources7
Beta strandi118 – 123Combined sources6
Helixi127 – 137Combined sources11
Helixi142 – 155Combined sources14
Helixi156 – 160Combined sources5
Turni162 – 164Combined sources3
Beta strandi166 – 170Combined sources5
Helixi174 – 184Combined sources11
Helixi186 – 191Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LVGX-ray2.10A1-198[»]
ProteinModelPortaliQ64520.
SMRiQ64520.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ64520.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 186Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST183

Sequence similaritiesi

Belongs to the guanylate kinase family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0707. Eukaryota.
COG0194. LUCA.
HOVERGENiHBG003344.
InParanoidiQ64520.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64520-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPRPVVLS GPSGAGKSTL LKKLFQEHSS IFGFSVSHTT RNPRPGEEDG
60 70 80 90 100
KDYYFVTREM MQRDIAAGDF IEHAEFSGNL YGTSKEAVRA VQAMNRICVL
110 120 130 140 150
DVDLQGVRSI KKTDLCPIYI FVQPPSLDVL EQRLRLRNTE TEESLAKRLA
160 170 180 190
AARTDMESSK EPGLFDLVII NDDLDKAYAT LKQALSEEIK KAQGTGHA
Length:198
Mass (Da):21,918
Last modified:January 23, 2007 - v2
Checksum:iF584E4B6521C607B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53514 mRNA. Translation: AAC52652.1.
BC024625 mRNA. Translation: AAH24625.1.
RefSeqiXP_006532308.1. XM_006532245.3.
XP_006532309.1. XM_006532246.2.
UniGeneiMm.3624.

Genome annotation databases

GeneIDi14923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53514 mRNA. Translation: AAC52652.1.
BC024625 mRNA. Translation: AAH24625.1.
RefSeqiXP_006532308.1. XM_006532245.3.
XP_006532309.1. XM_006532246.2.
UniGeneiMm.3624.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LVGX-ray2.10A1-198[»]
ProteinModelPortaliQ64520.
SMRiQ64520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64520. 2 interactors.
MINTiMINT-4099756.
STRINGi10090.ENSMUSP00000127566.

PTM databases

iPTMnetiQ64520.
PhosphoSitePlusiQ64520.

Proteomic databases

EPDiQ64520.
MaxQBiQ64520.
PaxDbiQ64520.
PRIDEiQ64520.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi14923.

Organism-specific databases

CTDi2987.
MGIiMGI:95871. Guk1.

Phylogenomic databases

eggNOGiKOG0707. Eukaryota.
COG0194. LUCA.
HOVERGENiHBG003344.
InParanoidiQ64520.

Enzyme and pathway databases

BRENDAi2.7.4.8. 3474.

Miscellaneous databases

ChiTaRSiGuk1. mouse.
EvolutionaryTraceiQ64520.
PROiQ64520.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020444.
CleanExiMM_GUK1.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKGUA_MOUSE
AccessioniPrimary (citable) accession number: Q64520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.