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Q64520

- KGUA_MOUSE

UniProt

Q64520 - KGUA_MOUSE

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Protein
Guanylate kinase
Gene
Guk1, Gmk
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for recycling GMP and indirectly, cGMP.

Catalytic activityi

ATP + GMP = ADP + GDP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei44 – 441 Inferred
Active sitei137 – 1371 Inferred
Active sitei148 – 1481 Inferred
Binding sitei171 – 1711ATP
Binding sitei172 – 1721ATP; via carbonyl oxygen

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. guanylate kinase activity Source: UniProtKB

GO - Biological processi

  1. ATP metabolic process Source: Ensembl
  2. GDP biosynthetic process Source: Ensembl
  3. GDP-mannose metabolic process Source: Ensembl
  4. GMP metabolic process Source: Ensembl
  5. dATP metabolic process Source: Ensembl
  6. dGDP biosynthetic process Source: Ensembl
  7. dGMP metabolic process Source: Ensembl
  8. glycoprotein transport Source: Ensembl
  9. purine nucleotide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196553. Abacavir metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate kinase (EC:2.7.4.8)
Alternative name(s):
GMP kinase
Gene namesi
Name:Guk1
Synonyms:Gmk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:95871. Guk1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 198197Guanylate kinase
PRO_0000170652Add
BLAST

Proteomic databases

PaxDbiQ64520.
PRIDEiQ64520.

PTM databases

PhosphoSiteiQ64520.

Expressioni

Gene expression databases

ArrayExpressiQ64520.
BgeeiQ64520.
CleanExiMM_GUK1.
GenevestigatoriQ64520.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

IntActiQ64520. 2 interactions.
MINTiMINT-4099756.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 104
Helixi17 – 2812
Turni29 – 313
Beta strandi32 – 343
Turni49 – 513
Helixi58 – 6710
Beta strandi70 – 767
Beta strandi79 – 846
Helixi85 – 939
Beta strandi97 – 1015
Helixi104 – 1107
Beta strandi118 – 1236
Helixi127 – 13711
Helixi142 – 15514
Helixi156 – 1605
Turni162 – 1643
Beta strandi166 – 1705
Helixi174 – 18411
Helixi186 – 1916

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LVGX-ray2.10A1-198[»]
ProteinModelPortaliQ64520.
SMRiQ64520. Positions 5-194.

Miscellaneous databases

EvolutionaryTraceiQ64520.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 186183Guanylate kinase-like
Add
BLAST

Sequence similaritiesi

Belongs to the guanylate kinase family.

Phylogenomic databases

eggNOGiCOG0194.
HOVERGENiHBG003344.
InParanoidiQ64520.
KOiK00942.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64520-1 [UniParc]FASTAAdd to Basket

« Hide

MAGPRPVVLS GPSGAGKSTL LKKLFQEHSS IFGFSVSHTT RNPRPGEEDG    50
KDYYFVTREM MQRDIAAGDF IEHAEFSGNL YGTSKEAVRA VQAMNRICVL 100
DVDLQGVRSI KKTDLCPIYI FVQPPSLDVL EQRLRLRNTE TEESLAKRLA 150
AARTDMESSK EPGLFDLVII NDDLDKAYAT LKQALSEEIK KAQGTGHA 198
Length:198
Mass (Da):21,918
Last modified:January 23, 2007 - v2
Checksum:iF584E4B6521C607B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U53514 mRNA. Translation: AAC52652.1.
BC024625 mRNA. Translation: AAH24625.1.
RefSeqiXP_006532308.1. XM_006532245.1.
XP_006532309.1. XM_006532246.1.
XP_006532310.1. XM_006532247.1.
UniGeneiMm.3624.

Genome annotation databases

GeneIDi14923.
KEGGimmu:14923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U53514 mRNA. Translation: AAC52652.1 .
BC024625 mRNA. Translation: AAH24625.1 .
RefSeqi XP_006532308.1. XM_006532245.1.
XP_006532309.1. XM_006532246.1.
XP_006532310.1. XM_006532247.1.
UniGenei Mm.3624.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1LVG X-ray 2.10 A 1-198 [» ]
ProteinModelPortali Q64520.
SMRi Q64520. Positions 5-194.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q64520. 2 interactions.
MINTi MINT-4099756.

PTM databases

PhosphoSitei Q64520.

Proteomic databases

PaxDbi Q64520.
PRIDEi Q64520.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 14923.
KEGGi mmu:14923.

Organism-specific databases

CTDi 2987.
MGIi MGI:95871. Guk1.

Phylogenomic databases

eggNOGi COG0194.
HOVERGENi HBG003344.
InParanoidi Q64520.
KOi K00942.

Enzyme and pathway databases

Reactomei REACT_196553. Abacavir metabolism.

Miscellaneous databases

ChiTaRSi GUK1. mouse.
EvolutionaryTracei Q64520.
PROi Q64520.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64520.
Bgeei Q64520.
CleanExi MM_GUK1.
Genevestigatori Q64520.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00625. Guanylate_kin. 1 hit.
[Graphical view ]
SMARTi SM00072. GuKc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR03263. guanyl_kin. 1 hit.
PROSITEi PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, characterization, and modeling of mouse and human guanylate kinases."
    Brady W.A., Kokoris M.S., Fitzgibbon M., Black M.E.
    J. Biol. Chem. 271:16734-16740(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "Structural characterization of the closed conformation of mouse guanylate kinase."
    Sekulic N., Shuvalova L., Spangenberg O., Konrad M., Lavie A.
    J. Biol. Chem. 277:30236-30243(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), ATP-BINDING SITES, ACTIVE SITE.

Entry informationi

Entry nameiKGUA_MOUSE
AccessioniPrimary (citable) accession number: Q64520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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