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Q64520 (KGUA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanylate kinase

EC=2.7.4.8
Alternative name(s):
GMP kinase
Gene names
Name:Guk1
Synonyms:Gmk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for recycling GMP and indirectly, cGMP.

Catalytic activity

ATP + GMP = ADP + GDP.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the guanylate kinase family.

Contains 1 guanylate kinase-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 198197Guanylate kinase
PRO_0000170652

Regions

Domain4 – 186183Guanylate kinase-like
Nucleotide binding11 – 188ATP

Sites

Active site441 Probable
Active site1371 Probable
Active site1481 Probable
Binding site1711ATP
Binding site1721ATP; via carbonyl oxygen

Secondary structure

................................... 198
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q64520 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: F584E4B6521C607B

FASTA19821,918
        10         20         30         40         50         60 
MAGPRPVVLS GPSGAGKSTL LKKLFQEHSS IFGFSVSHTT RNPRPGEEDG KDYYFVTREM 

        70         80         90        100        110        120 
MQRDIAAGDF IEHAEFSGNL YGTSKEAVRA VQAMNRICVL DVDLQGVRSI KKTDLCPIYI 

       130        140        150        160        170        180 
FVQPPSLDVL EQRLRLRNTE TEESLAKRLA AARTDMESSK EPGLFDLVII NDDLDKAYAT 

       190 
LKQALSEEIK KAQGTGHA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, characterization, and modeling of mouse and human guanylate kinases."
Brady W.A., Kokoris M.S., Fitzgibbon M., Black M.E.
J. Biol. Chem. 271:16734-16740(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[3]"Structural characterization of the closed conformation of mouse guanylate kinase."
Sekulic N., Shuvalova L., Spangenberg O., Konrad M., Lavie A.
J. Biol. Chem. 277:30236-30243(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), ATP-BINDING SITES, ACTIVE SITE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U53514 mRNA. Translation: AAC52652.1.
BC024625 mRNA. Translation: AAH24625.1.
RefSeqXP_006532308.1. XM_006532245.1.
XP_006532309.1. XM_006532246.1.
XP_006532310.1. XM_006532247.1.
UniGeneMm.3624.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LVGX-ray2.10A1-198[»]
ProteinModelPortalQ64520.
SMRQ64520. Positions 5-194.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ64520. 2 interactions.
MINTMINT-4099756.

PTM databases

PhosphoSiteQ64520.

Proteomic databases

PaxDbQ64520.
PRIDEQ64520.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID14923.
KEGGmmu:14923.

Organism-specific databases

CTD2987.
MGIMGI:95871. Guk1.

Phylogenomic databases

eggNOGCOG0194.
HOVERGENHBG003344.
InParanoidQ64520.
KOK00942.

Gene expression databases

ArrayExpressQ64520.
BgeeQ64520.
CleanExMM_GUK1.
GenevestigatorQ64520.

Family and domain databases

Gene3D3.40.50.300. 3 hits.
InterProIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR03263. guanyl_kin. 1 hit.
PROSITEPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGUK1. mouse.
EvolutionaryTraceQ64520.
PROQ64520.
SOURCESearch...

Entry information

Entry nameKGUA_MOUSE
AccessionPrimary (citable) accession number: Q64520
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot