Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Names and origin

Protein names

Recommended name:
    Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
      Short name=SR Ca(2+)-ATPase 3
      Short name=SERCA3
    EC=3.6.3.8
Alternative name(s):
    Calcium pump 3

Gene names

Name:

Atp2a3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	1038 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Catalytic activity	ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
Subcellular location	Nucleus membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Sarcoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIA subfamily.

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Endoplasmic reticulum Membrane Nucleus Sarcoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	ATP-binding Calcium Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-KW sarcoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform SERCA3B (identifier: Q64518-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform SERCA3A (identifier: Q64518-2) The sequence of this isoform differs from the canonical sequence as follows: 994-1038: GVLGTFMQARSRQLPTTSRTPYHTGKKGPEVNPGSRGESPVWPSD → EKKDLK

Isoform SERCA3C (identifier: Q64518-3) The sequence of this isoform differs from the canonical sequence as follows: 1019-1038: KKGPEVNPGSRGESPVWPSD → LACKKKT

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1038

1038

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

PRO_0000046203

Regions

Topological domain

1 – 48

48

Cytoplasmic By similarity

Transmembrane

49 – 69

21

1 By similarity

Topological domain

70 – 89

20

Lumenal By similarity

Transmembrane

90 – 110

21

2 By similarity

Topological domain

111 – 253

143

Cytoplasmic By similarity

Transmembrane

254 – 273

20

3 By similarity

Topological domain

274 – 295

22

Lumenal By similarity

Transmembrane

296 – 313

18

4 By similarity

Topological domain

314 – 757

444

Cytoplasmic By similarity

Transmembrane

758 – 777

20

5 By similarity

Topological domain

778 – 787

10

Lumenal By similarity

Transmembrane

788 – 808

21

6 By similarity

Topological domain

809 – 828

20

Cytoplasmic By similarity

Transmembrane

829 – 851

23

7 By similarity

Topological domain

852 – 897

46

Lumenal By similarity

Transmembrane

898 – 917

20

8 By similarity

Topological domain

918 – 930

13

Cytoplasmic By similarity

Transmembrane

931 – 949

19

9 By similarity

Topological domain

950 – 964

15

Lumenal By similarity

Transmembrane

965 – 985

21

10 By similarity

Topological domain

986 – 1038

53

Cytoplasmic By similarity

Sites

Active site

351

1

4-aspartylphosphate intermediate By similarity

Metal binding

304

1

Calcium 2; via carbonyl oxygen By similarity

Metal binding

305

1

Calcium 2; via carbonyl oxygen By similarity

Metal binding

307

1

Calcium 2; via carbonyl oxygen By similarity

Metal binding

309

1

Calcium 2 By similarity

Metal binding

703

1

Magnesium By similarity

Metal binding

707

1

Magnesium By similarity

Metal binding

768

1

Calcium 1 By similarity

Metal binding

771

1

Calcium 1 By similarity

Metal binding

796

1

Calcium 2 By similarity

Metal binding

799

1

Calcium 1 By similarity

Metal binding

800

1

Calcium 1 By similarity

Metal binding

800

1

Calcium 2 By similarity

Metal binding

908

1

Calcium 1 By similarity

Binding site

515

1

ATP By similarity

Amino acid modifications

Modified residue

1

N-acetylmethionine By similarity

Natural variations

Alternative sequence

994 – 1038

45

GVLGT…VWPSD → EKKDLK in isoform SERCA3A.

VSP_000369

Alternative sequence

1019 – 1038

20

KKGPE…VWPSD → LACKKKT in isoform SERCA3C.

VSP_000370

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Sequences

Sequence		Length	Mass (Da)
Isoform SERCA3B [UniParc]. Last modified October 18, 2001. Version 2. Checksum: 47C3763E843EEA8E Show »	FASTA	1,038	113,608
10 20 30 40 50 60 MEEAHLLSAA DVLRRFSVTA EGGLSLEQVT DARERYGPNE LPTEEGKSLW ELVVEQFEDL 70 80 90 100 110 120 LVRILLLAAL VSFVLAWFEE GEETTTAFVE PLVIMLILVA NAIVGVWQER NAESAIEALK 130 140 150 160 170 180 EYEPEMGKVI RSDRKGVQRI RARDIVPGDI VEVAVGDKVP ADLRLIEIKS TTLRVDQSIL 190 200 210 220 230 240 TGESVSVTKH TDAIPDPRAV NQDKKNMLFS GTNIASGKAL GVAVATGLQT ELGKIRSQMA 250 260 270 280 290 300 AVEPERTPLQ RKLDEFGRQL SHAISVICVA VWVINIGHFA DPAHGGSWLR GAVYYFKIAV 310 320 330 340 350 360 ALAVAAIPEG LPAVITTCLA LGTRRMARKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 370 380 390 400 410 420 MSVCRMFVVA EAEAGTCRLH EFTISGTTYT PEGEVRQGEQ PVRCGQFDGL VELATICALC 430 440 450 460 470 480 NDSALDYNEA KGVYEKVGEA TETALTCLVE KMNVFDTDLK GLSRVERAGA CNSVIKQLMR 490 500 510 520 530 540 KEFTLEFSRD RKSMSVYCTP TRADPKVQGS KMFVKGAPES VIERCSSVRV GSRTAPLSTT 550 560 570 580 590 600 SREHILAKIR DWGSGSDTLR CLALATRDTP PRKEDMHLDD CSRFVQYETD LTFVGCVGML 610 620 630 640 650 660 DPPRPEVAAC ITRCSRAGIR VVMITGDNKG TAVAICRRLG IFGDTEDVLG KAYTGREFDD 670 680 690 700 710 720 LSPEQQRQAC RTARCFARVE PAHKSRIVEN LQSFNEITAM TGDGVNDAPA LKKAEIGIAM 730 740 750 760 770 780 GSGTAVAKSA AEMVLSDDNF ASIVAAVEEG RAIYNNMKQF IRYLISSNVG EVVCIFLTAI 790 800 810 820 830 840 LGLPEALIPV QLLWVNLVTD GLPATALGFN PPDLDIMEKP PRNPREALIS GWLFFRYLAI 850 860 870 880 890 900 GVYVGLATVA AATWWFLYDA EGPQVTFYQL RNFLKCSEDN PLFAGIDCKV FESRFPTTMA 910 920 930 940 950 960 LSVLVTIEMC NALNSVSENQ SLLRMPPWLN PWLLGAVVMS MALHFLILLV PPLPLIFQVT 970 980 990 1000 1010 1020 PLSGRQWGVV LQMSLPVILL DEALKYLSRN HMDGVLGTFM QARSRQLPTT SRTPYHTGKK 1030 GPEVNPGSRG ESPVWPSD « Hide
Isoform SERCA3A. Checksum: F65071FDE732B7E7 Show »	FASTA	999	109,500
10 20 30 40 50 60 MEEAHLLSAA DVLRRFSVTA EGGLSLEQVT DARERYGPNE LPTEEGKSLW ELVVEQFEDL 70 80 90 100 110 120 LVRILLLAAL VSFVLAWFEE GEETTTAFVE PLVIMLILVA NAIVGVWQER NAESAIEALK 130 140 150 160 170 180 EYEPEMGKVI RSDRKGVQRI RARDIVPGDI VEVAVGDKVP ADLRLIEIKS TTLRVDQSIL 190 200 210 220 230 240 TGESVSVTKH TDAIPDPRAV NQDKKNMLFS GTNIASGKAL GVAVATGLQT ELGKIRSQMA 250 260 270 280 290 300 AVEPERTPLQ RKLDEFGRQL SHAISVICVA VWVINIGHFA DPAHGGSWLR GAVYYFKIAV 310 320 330 340 350 360 ALAVAAIPEG LPAVITTCLA LGTRRMARKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 370 380 390 400 410 420 MSVCRMFVVA EAEAGTCRLH EFTISGTTYT PEGEVRQGEQ PVRCGQFDGL VELATICALC 430 440 450 460 470 480 NDSALDYNEA KGVYEKVGEA TETALTCLVE KMNVFDTDLK GLSRVERAGA CNSVIKQLMR 490 500 510 520 530 540 KEFTLEFSRD RKSMSVYCTP TRADPKVQGS KMFVKGAPES VIERCSSVRV GSRTAPLSTT 550 560 570 580 590 600 SREHILAKIR DWGSGSDTLR CLALATRDTP PRKEDMHLDD CSRFVQYETD LTFVGCVGML 610 620 630 640 650 660 DPPRPEVAAC ITRCSRAGIR VVMITGDNKG TAVAICRRLG IFGDTEDVLG KAYTGREFDD 670 680 690 700 710 720 LSPEQQRQAC RTARCFARVE PAHKSRIVEN LQSFNEITAM TGDGVNDAPA LKKAEIGIAM 730 740 750 760 770 780 GSGTAVAKSA AEMVLSDDNF ASIVAAVEEG RAIYNNMKQF IRYLISSNVG EVVCIFLTAI 790 800 810 820 830 840 LGLPEALIPV QLLWVNLVTD GLPATALGFN PPDLDIMEKP PRNPREALIS GWLFFRYLAI 850 860 870 880 890 900 GVYVGLATVA AATWWFLYDA EGPQVTFYQL RNFLKCSEDN PLFAGIDCKV FESRFPTTMA 910 920 930 940 950 960 LSVLVTIEMC NALNSVSENQ SLLRMPPWLN PWLLGAVVMS MALHFLILLV PPLPLIFQVT 970 980 990 PLSGRQWGVV LQMSLPVILL DEALKYLSRN HMDEKKDLK « Hide
Isoform SERCA3C. Checksum: 821939C5B3FEDF36 Show »	FASTA	1,025	112,276
10 20 30 40 50 60 MEEAHLLSAA DVLRRFSVTA EGGLSLEQVT DARERYGPNE LPTEEGKSLW ELVVEQFEDL 70 80 90 100 110 120 LVRILLLAAL VSFVLAWFEE GEETTTAFVE PLVIMLILVA NAIVGVWQER NAESAIEALK 130 140 150 160 170 180 EYEPEMGKVI RSDRKGVQRI RARDIVPGDI VEVAVGDKVP ADLRLIEIKS TTLRVDQSIL 190 200 210 220 230 240 TGESVSVTKH TDAIPDPRAV NQDKKNMLFS GTNIASGKAL GVAVATGLQT ELGKIRSQMA 250 260 270 280 290 300 AVEPERTPLQ RKLDEFGRQL SHAISVICVA VWVINIGHFA DPAHGGSWLR GAVYYFKIAV 310 320 330 340 350 360 ALAVAAIPEG LPAVITTCLA LGTRRMARKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 370 380 390 400 410 420 MSVCRMFVVA EAEAGTCRLH EFTISGTTYT PEGEVRQGEQ PVRCGQFDGL VELATICALC 430 440 450 460 470 480 NDSALDYNEA KGVYEKVGEA TETALTCLVE KMNVFDTDLK GLSRVERAGA CNSVIKQLMR 490 500 510 520 530 540 KEFTLEFSRD RKSMSVYCTP TRADPKVQGS KMFVKGAPES VIERCSSVRV GSRTAPLSTT 550 560 570 580 590 600 SREHILAKIR DWGSGSDTLR CLALATRDTP PRKEDMHLDD CSRFVQYETD LTFVGCVGML 610 620 630 640 650 660 DPPRPEVAAC ITRCSRAGIR VVMITGDNKG TAVAICRRLG IFGDTEDVLG KAYTGREFDD 670 680 690 700 710 720 LSPEQQRQAC RTARCFARVE PAHKSRIVEN LQSFNEITAM TGDGVNDAPA LKKAEIGIAM 730 740 750 760 770 780 GSGTAVAKSA AEMVLSDDNF ASIVAAVEEG RAIYNNMKQF IRYLISSNVG EVVCIFLTAI 790 800 810 820 830 840 LGLPEALIPV QLLWVNLVTD GLPATALGFN PPDLDIMEKP PRNPREALIS GWLFFRYLAI 850 860 870 880 890 900 GVYVGLATVA AATWWFLYDA EGPQVTFYQL RNFLKCSEDN PLFAGIDCKV FESRFPTTMA 910 920 930 940 950 960 LSVLVTIEMC NALNSVSENQ SLLRMPPWLN PWLLGAVVMS MALHFLILLV PPLPLIFQVT 970 980 990 1000 1010 1020 PLSGRQWGVV LQMSLPVILL DEALKYLSRN HMDGVLGTFM QARSRQLPTT SRTPYHTGLA CKKKT « Hide

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References

[1]	Tokuyama Y., Chen X., Roe M.W., Bell G.I. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SERCA3A AND SERCA3B). Strain: C57BL/6.
[2]	"Structure of the human sarco/endoplasmic reticulum Ca2+-ATPase 3 gene. Promoter analysis and alternative splicing of the SERCA3 pre-mRNA." Dode L., De Greef C., Mountian I., Attard M., Town M.M., Casteels R., Wuytack F. J. Biol. Chem. 273:13982-13994(1998) [PubMed: 9593748] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SERCA3A), ALTERNATIVE SPLICING.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	U49394 mRNA. Translation: AAB04099.1. U49393 mRNA. Translation: AAB04098.1. Y15734, Y15735 Genomic DNA. Translation: CAA75744.1. Y15734, Y15735 Genomic DNA. Translation: CAA75745.1. Y15734, Y15735 Genomic DNA. Translation: CAA75743.1. Y15736 Genomic DNA. Translation: CAA75746.1.
IPI	IPI00311200. IPI00336889. IPI00944111.
RefSeq	NP_001156809.1. NP_058025.2.
UniGene	Mm.6306
3D structure databases
SMR	Q64518. Positions 1-994.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64518.
Proteomic databases
PRIDE	Q64518.
Genome annotation databases
Ensembl	ENSMUST00000021142; ENSMUSP00000021142; ENSMUSG00000020788; Mus musculus. [Genome view] ENSMUST00000108485; ENSMUSP00000104125; ENSMUSG00000020788; Mus musculus. [Genome view]
GeneID	53313.
Organism-specific databases
CTD	53313.
MGI	MGI:1194503. Atp2a3.
Phylogenomic databases
eggNOG	maNOG16465.
HOGENOM	HBG456486.
HOVERGEN	Q64518.
InParanoid	Q64518.
PhylomeDB	Q64518.
Enzyme and pathway databases
BRENDA	3.6.3.8. 244.
Gene expression databases
ArrayExpress	Q64518.
Bgee	Q64518.
CleanEx	MM_ATP2A3.
Genevestigator	Q64518.
GermOnline	ENSMUSG00000020788. Mus musculus.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR005782. ATPase_P-typ_Ca-transp. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01116. ATPase-IIA1_Ca. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

AT2A3_MOUSE

Accession

Primary (citable) accession number: Q64518
Secondary accession number(s): O70625, Q64517

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	October 18, 2001
Last modified:	February 9, 2010
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents