SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q64518

- AT2A3_MOUSE

UniProt

Q64518 - AT2A3_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Gene
Atp2a3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi304 – 3041Calcium 2; via carbonyl oxygen By similarity
Metal bindingi305 – 3051Calcium 2; via carbonyl oxygen By similarity
Metal bindingi307 – 3071Calcium 2; via carbonyl oxygen By similarity
Metal bindingi309 – 3091Calcium 2 By similarity
Active sitei351 – 35114-aspartylphosphate intermediate By similarity
Binding sitei515 – 5151ATP By similarity
Metal bindingi703 – 7031Magnesium By similarity
Metal bindingi707 – 7071Magnesium By similarity
Metal bindingi768 – 7681Calcium 1 By similarity
Metal bindingi771 – 7711Calcium 1 By similarity
Metal bindingi796 – 7961Calcium 2 By similarity
Metal bindingi799 – 7991Calcium 1 By similarity
Metal bindingi800 – 8001Calcium 1 By similarity
Metal bindingi800 – 8001Calcium 2 By similarity
Metal bindingi908 – 9081Calcium 1 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium-transporting ATPase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 (EC:3.6.3.8)
Short name:
SERCA3
Short name:
SR Ca(2+)-ATPase 3
Alternative name(s):
Calcium pump 3
Gene namesi
Name:Atp2a3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1194503. Atp2a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848Cytoplasmic By similarityAdd
BLAST
Transmembranei49 – 6921Helical; Name=1; By similarityAdd
BLAST
Topological domaini70 – 8920Lumenal By similarityAdd
BLAST
Transmembranei90 – 11021Helical; Name=2; By similarityAdd
BLAST
Topological domaini111 – 253143Cytoplasmic By similarityAdd
BLAST
Transmembranei254 – 27320Helical; Name=3; By similarityAdd
BLAST
Topological domaini274 – 29522Lumenal By similarityAdd
BLAST
Transmembranei296 – 31318Helical; Name=4; By similarityAdd
BLAST
Topological domaini314 – 757444Cytoplasmic By similarityAdd
BLAST
Transmembranei758 – 77720Helical; Name=5; By similarityAdd
BLAST
Topological domaini778 – 78710Lumenal By similarity
Transmembranei788 – 80821Helical; Name=6; By similarityAdd
BLAST
Topological domaini809 – 82820Cytoplasmic By similarityAdd
BLAST
Transmembranei829 – 85123Helical; Name=7; By similarityAdd
BLAST
Topological domaini852 – 89746Lumenal By similarityAdd
BLAST
Transmembranei898 – 91720Helical; Name=8; By similarityAdd
BLAST
Topological domaini918 – 93013Cytoplasmic By similarityAdd
BLAST
Transmembranei931 – 94919Helical; Name=9; By similarityAdd
BLAST
Topological domaini950 – 96415Lumenal By similarityAdd
BLAST
Transmembranei965 – 98521Helical; Name=10; By similarityAdd
BLAST
Topological domaini986 – 103853Cytoplasmic By similarityAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. nuclear membrane Source: UniProtKB-SubCell
  4. sarcoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10381038Sarcoplasmic/endoplasmic reticulum calcium ATPase 3PRO_0000046203Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei662 – 6621Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ64518.
PaxDbiQ64518.
PRIDEiQ64518.

PTM databases

PhosphoSiteiQ64518.

Expressioni

Gene expression databases

ArrayExpressiQ64518.
BgeeiQ64518.
CleanExiMM_ATP2A3.
GenevestigatoriQ64518.

Interactioni

Protein-protein interaction databases

BioGridi207277. 1 interaction.
IntActiQ64518. 1 interaction.
MINTiMINT-4088610.

Structurei

3D structure databases

ProteinModelPortaliQ64518.
SMRiQ64518. Positions 1-994.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00560000076887.
HOGENOMiHOG000265621.
HOVERGENiHBG105648.
InParanoidiQ8VD16.
KOiK05853.
OMAiACITRCY.
TreeFamiTF300651.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform SERCA3B (identifier: Q64518-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEEAHLLSAA DVLRRFSVTA EGGLSLEQVT DARERYGPNE LPTEEGKSLW     50
ELVVEQFEDL LVRILLLAAL VSFVLAWFEE GEETTTAFVE PLVIMLILVA 100
NAIVGVWQER NAESAIEALK EYEPEMGKVI RSDRKGVQRI RARDIVPGDI 150
VEVAVGDKVP ADLRLIEIKS TTLRVDQSIL TGESVSVTKH TDAIPDPRAV 200
NQDKKNMLFS GTNIASGKAL GVAVATGLQT ELGKIRSQMA AVEPERTPLQ 250
RKLDEFGRQL SHAISVICVA VWVINIGHFA DPAHGGSWLR GAVYYFKIAV 300
ALAVAAIPEG LPAVITTCLA LGTRRMARKN AIVRSLPSVE TLGCTSVICS 350
DKTGTLTTNQ MSVCRMFVVA EAEAGTCRLH EFTISGTTYT PEGEVRQGEQ 400
PVRCGQFDGL VELATICALC NDSALDYNEA KGVYEKVGEA TETALTCLVE 450
KMNVFDTDLK GLSRVERAGA CNSVIKQLMR KEFTLEFSRD RKSMSVYCTP 500
TRADPKVQGS KMFVKGAPES VIERCSSVRV GSRTAPLSTT SREHILAKIR 550
DWGSGSDTLR CLALATRDTP PRKEDMHLDD CSRFVQYETD LTFVGCVGML 600
DPPRPEVAAC ITRCSRAGIR VVMITGDNKG TAVAICRRLG IFGDTEDVLG 650
KAYTGREFDD LSPEQQRQAC RTARCFARVE PAHKSRIVEN LQSFNEITAM 700
TGDGVNDAPA LKKAEIGIAM GSGTAVAKSA AEMVLSDDNF ASIVAAVEEG 750
RAIYNNMKQF IRYLISSNVG EVVCIFLTAI LGLPEALIPV QLLWVNLVTD 800
GLPATALGFN PPDLDIMEKP PRNPREALIS GWLFFRYLAI GVYVGLATVA 850
AATWWFLYDT EGPQVTFYQL RNFLKCSEDN PLFAGIDCKV FESRFPTTMA 900
LSVLVTIEMC NALNSVSENQ SLLRMPPWLN PWLLGAVVMS MALHFLILLV 950
PPLPLIFQVT PLSGRQWGVV LQMSLPVILL DEALKYLSRN HMDGVLGTFM 1000
QARSRQLPTT SRTPYHTGKK GPEVNPGSRG ESPVWPSD 1038
Length:1,038
Mass (Da):113,638
Last modified:July 27, 2011 - v3
Checksum:iA7D6798E8B814F94
GO
Isoform SERCA3A (identifier: Q64518-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     994-1038: GVLGTFMQARSRQLPTTSRTPYHTGKKGPEVNPGSRGESPVWPSD → EKKDLK

Show »
Length:999
Mass (Da):109,530
Checksum:iF64B7046E792B6E7
GO
Isoform SERCA3C (identifier: Q64518-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1019-1038: KKGPEVNPGSRGESPVWPSD → LACKKKT

Show »
Length:1,025
Mass (Da):112,306
Checksum:i29A928D4B3F278F0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei994 – 103845GVLGT…VWPSD → EKKDLK in isoform SERCA3A. VSP_000369Add
BLAST
Alternative sequencei1019 – 103820KKGPE…VWPSD → LACKKKT in isoform SERCA3C. VSP_000370Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti860 – 8601T → A in AAB04098. 1 Publication
Sequence conflicti860 – 8601T → A in AAB04099. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U49394 mRNA. Translation: AAB04099.1.
U49393 mRNA. Translation: AAB04098.1.
CH466596 Genomic DNA. Translation: EDL12704.1.
BC017639 mRNA. Translation: AAH17639.1.
Y15734, Y15735 Genomic DNA. Translation: CAA75744.1.
Y15734, Y15735 Genomic DNA. Translation: CAA75745.1.
Y15734, Y15735 Genomic DNA. Translation: CAA75743.1.
Y15736 Genomic DNA. Translation: CAA75746.1.
CCDSiCCDS24991.1. [Q64518-1]
CCDS48843.1. [Q64518-2]
RefSeqiNP_001156809.1. NM_001163337.1.
NP_058025.2. NM_016745.3. [Q64518-1]
UniGeneiMm.6306.

Genome annotation databases

EnsembliENSMUST00000021142; ENSMUSP00000021142; ENSMUSG00000020788. [Q64518-1]
ENSMUST00000108485; ENSMUSP00000104125; ENSMUSG00000020788. [Q64518-2]
GeneIDi53313.
KEGGimmu:53313.
UCSCiuc007jzo.2. mouse. [Q64518-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U49394 mRNA. Translation: AAB04099.1 .
U49393 mRNA. Translation: AAB04098.1 .
CH466596 Genomic DNA. Translation: EDL12704.1 .
BC017639 mRNA. Translation: AAH17639.1 .
Y15734 , Y15735 Genomic DNA. Translation: CAA75744.1 .
Y15734 , Y15735 Genomic DNA. Translation: CAA75745.1 .
Y15734 , Y15735 Genomic DNA. Translation: CAA75743.1 .
Y15736 Genomic DNA. Translation: CAA75746.1 .
CCDSi CCDS24991.1. [Q64518-1 ]
CCDS48843.1. [Q64518-2 ]
RefSeqi NP_001156809.1. NM_001163337.1.
NP_058025.2. NM_016745.3. [Q64518-1 ]
UniGenei Mm.6306.

3D structure databases

ProteinModelPortali Q64518.
SMRi Q64518. Positions 1-994.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207277. 1 interaction.
IntActi Q64518. 1 interaction.
MINTi MINT-4088610.

PTM databases

PhosphoSitei Q64518.

Proteomic databases

MaxQBi Q64518.
PaxDbi Q64518.
PRIDEi Q64518.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021142 ; ENSMUSP00000021142 ; ENSMUSG00000020788 . [Q64518-1 ]
ENSMUST00000108485 ; ENSMUSP00000104125 ; ENSMUSG00000020788 . [Q64518-2 ]
GeneIDi 53313.
KEGGi mmu:53313.
UCSCi uc007jzo.2. mouse. [Q64518-1 ]

Organism-specific databases

CTDi 489.
MGIi MGI:1194503. Atp2a3.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00560000076887.
HOGENOMi HOG000265621.
HOVERGENi HBG105648.
InParanoidi Q8VD16.
KOi K05853.
OMAi ACITRCY.
TreeFami TF300651.

Miscellaneous databases

ChiTaRSi ATP2A3. mouse.
NextBioi 310097.
PROi Q64518.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64518.
Bgeei Q64518.
CleanExi MM_ATP2A3.
Genevestigatori Q64518.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Tokuyama Y., Chen X., Roe M.W., Bell G.I.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SERCA3A AND SERCA3B).
    Strain: C57BL/6.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "Structure of the human sarco/endoplasmic reticulum Ca2+-ATPase 3 gene. Promoter analysis and alternative splicing of the SERCA3 pre-mRNA."
    Dode L., De Greef C., Mountian I., Attard M., Town M.M., Casteels R., Wuytack F.
    J. Biol. Chem. 273:13982-13994(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SERCA3A), ALTERNATIVE SPLICING.

Entry informationi

Entry nameiAT2A3_MOUSE
AccessioniPrimary (citable) accession number: Q64518
Secondary accession number(s): O70625, Q64517, Q8VD16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi