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Protein

Glycerol kinase

Gene

Gk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism.By similarity

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase 2 (Gk2), Glycerol kinase (Gk), Putative glycerol kinase 5 (Gk5)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201SubstrateBy similarity
Binding sitei24 – 241ATPBy similarity
Binding sitei94 – 941SubstrateBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Binding sitei265 – 2651SubstrateBy similarity
Binding sitei287 – 2871ATPBy similarity
Binding sitei332 – 3321ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi433 – 4375ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glycerol kinase activity Source: MGI

GO - Biological processi

  • glucose homeostasis Source: MGI
  • glycerol-3-phosphate biosynthetic process Source: GO_Central
  • glycerol catabolic process Source: UniProtKB-UniPathway
  • glycerol metabolic process Source: MGI
  • regulation of fatty acid metabolic process Source: MGI
  • response to cold Source: Ensembl
  • response to drug Source: Ensembl
  • response to ethanol Source: Ensembl
  • triglyceride metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinase (EC:2.7.1.30)
Short name:
GK
Short name:
Glycerokinase
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Gene namesi
Name:Gk
Synonyms:Gyk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:106594. Gk.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • extracellular exosome Source: Ensembl
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 559559Glycerol kinasePRO_0000059538Add
BLAST

Proteomic databases

EPDiQ64516.
MaxQBiQ64516.
PaxDbiQ64516.
PeptideAtlasiQ64516.
PRIDEiQ64516.

PTM databases

iPTMnetiQ64516.
PhosphoSiteiQ64516.
SwissPalmiQ64516.

Expressioni

Gene expression databases

BgeeiQ64516.
CleanExiMM_GYK.
ExpressionAtlasiQ64516. baseline and differential.
GenevisibleiQ64516. MM.

Interactioni

Protein-protein interaction databases

BioGridi200130. 1 interaction.
IntActiQ64516. 4 interactions.
MINTiMINT-1855905.
STRINGi10090.ENSMUSP00000119564.

Structurei

3D structure databases

ProteinModelPortaliQ64516.
SMRiQ64516. Positions 13-520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FGGY kinase family.Curated

Phylogenomic databases

eggNOGiKOG2517. Eukaryota.
COG0554. LUCA.
GeneTreeiENSGT00530000063143.
HOGENOMiHOG000222134.
HOVERGENiHBG002451.
InParanoidiQ64516.
KOiK00864.
OMAiETYIMAI.
OrthoDBiEOG7VTDMZ.
PhylomeDBiQ64516.
TreeFamiTF321504.

Family and domain databases

InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q64516-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAKKAVLG PLVGAVDQGT SSTRFLVFNS KTAELLSHHQ VEIKQEFPRE
60 70 80 90 100
GWVEQDPKEI LQSVYECIEK TCEKLGQLNI DISNIKAIGV SNQRETTVVW
110 120 130 140 150
DKVTGEPLYN AVVWLDLRTQ STVENLSKRI PGNNNFVKSK TGLPLSTYFS
160 170 180 190 200
AVKLRWLLDN VKKVQEAVEE NRALFGTIDS WLIWSLTGGI HGGVHCTDVT
210 220 230 240 250
NASRTMLFNI HSLEWDKELC EFFGIPMEIL PNVRSSSEIY GLMKISHSLK
260 270 280 290 300
AGALEGVPIS GCLGDQSAAL VGQMCFQDGQ AKNTYGTGCF LLCNTGHKCV
310 320 330 340 350
FSEHGLLTTV AYKLGRDKPV YYALEGSVAI AGAVIRWLRD NLGIIKSSEE
360 370 380 390 400
IEKLAKEVGT SYGCYFVPAF SGLYAPYWEP SARGIICGLT QFTNKCHIAF
410 420 430 440 450
AALEAVCFQT REILDAMNRD CGIPLSHLQV DGGMTSNKIL MQLQADILYI
460 470 480 490 500
PVVKPSMPET TALGAAMAAG AAEGVGVWSL EPEDLSAVTM ERFEPQINAE
510 520 530 540 550
ESEIRYSTWK KAVMKSIGWV TTQSPESGDP SIFCSLPLGF FIVSSMVMLI

GARYISGIP
Length:559
Mass (Da):61,227
Last modified:July 22, 2008 - v2
Checksum:i09A58CB47AB5CD41
GO
Isoform 2 (identifier: Q64516-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     245-250: Missing.

Show »
Length:553
Mass (Da):60,562
Checksum:i386A4ED070B2A9CC
GO
Isoform 1 (identifier: Q64516-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     245-250: Missing.
     528-556: Missing.

Show »
Length:524
Mass (Da):57,458
Checksum:iC3E1C56887683B55
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551Q → R in BAC31861 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei245 – 2506Missing in isoform 1 and isoform 2. 3 PublicationsVSP_034650
Alternative sequencei528 – 55629Missing in isoform 1. 2 PublicationsVSP_034651Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48403 mRNA. Translation: AAC52824.1.
AK044308 mRNA. Translation: BAC31861.1.
AK088373 mRNA. Translation: BAC40312.1.
AL645567, AL672056 Genomic DNA. Translation: CAM16667.1.
AL645567, AL672056 Genomic DNA. Translation: CAM16668.1.
AL672056, AL645567 Genomic DNA. Translation: CAM25261.1.
AL672056, AL645567 Genomic DNA. Translation: CAM25262.1.
BC003767 mRNA. Translation: AAH03767.1.
CCDSiCCDS41050.1. [Q64516-2]
CCDS53126.1. [Q64516-1]
CCDS81149.1. [Q64516-3]
RefSeqiNP_001281069.1. NM_001294140.1. [Q64516-3]
NP_032220.1. NM_008194.3. [Q64516-1]
NP_997609.1. NM_212444.2. [Q64516-2]
UniGeneiMm.246682.

Genome annotation databases

EnsembliENSMUST00000026039; ENSMUSP00000026039; ENSMUSG00000025059. [Q64516-1]
ENSMUST00000113978; ENSMUSP00000109611; ENSMUSG00000025059. [Q64516-3]
ENSMUST00000156390; ENSMUSP00000119564; ENSMUSG00000025059. [Q64516-2]
GeneIDi14933.
KEGGimmu:14933.
UCSCiuc009trv.1. mouse. [Q64516-2]
uc009trw.1. mouse. [Q64516-1]
uc009trx.1. mouse. [Q64516-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48403 mRNA. Translation: AAC52824.1.
AK044308 mRNA. Translation: BAC31861.1.
AK088373 mRNA. Translation: BAC40312.1.
AL645567, AL672056 Genomic DNA. Translation: CAM16667.1.
AL645567, AL672056 Genomic DNA. Translation: CAM16668.1.
AL672056, AL645567 Genomic DNA. Translation: CAM25261.1.
AL672056, AL645567 Genomic DNA. Translation: CAM25262.1.
BC003767 mRNA. Translation: AAH03767.1.
CCDSiCCDS41050.1. [Q64516-2]
CCDS53126.1. [Q64516-1]
CCDS81149.1. [Q64516-3]
RefSeqiNP_001281069.1. NM_001294140.1. [Q64516-3]
NP_032220.1. NM_008194.3. [Q64516-1]
NP_997609.1. NM_212444.2. [Q64516-2]
UniGeneiMm.246682.

3D structure databases

ProteinModelPortaliQ64516.
SMRiQ64516. Positions 13-520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200130. 1 interaction.
IntActiQ64516. 4 interactions.
MINTiMINT-1855905.
STRINGi10090.ENSMUSP00000119564.

PTM databases

iPTMnetiQ64516.
PhosphoSiteiQ64516.
SwissPalmiQ64516.

Proteomic databases

EPDiQ64516.
MaxQBiQ64516.
PaxDbiQ64516.
PeptideAtlasiQ64516.
PRIDEiQ64516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026039; ENSMUSP00000026039; ENSMUSG00000025059. [Q64516-1]
ENSMUST00000113978; ENSMUSP00000109611; ENSMUSG00000025059. [Q64516-3]
ENSMUST00000156390; ENSMUSP00000119564; ENSMUSG00000025059. [Q64516-2]
GeneIDi14933.
KEGGimmu:14933.
UCSCiuc009trv.1. mouse. [Q64516-2]
uc009trw.1. mouse. [Q64516-1]
uc009trx.1. mouse. [Q64516-3]

Organism-specific databases

CTDi2710.
MGIiMGI:106594. Gk.

Phylogenomic databases

eggNOGiKOG2517. Eukaryota.
COG0554. LUCA.
GeneTreeiENSGT00530000063143.
HOGENOMiHOG000222134.
HOVERGENiHBG002451.
InParanoidiQ64516.
KOiK00864.
OMAiETYIMAI.
OrthoDBiEOG7VTDMZ.
PhylomeDBiQ64516.
TreeFamiTF321504.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
ReactomeiR-MMU-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ64516.
SOURCEiSearch...

Gene expression databases

BgeeiQ64516.
CleanExiMM_GYK.
ExpressionAtlasiQ64516. baseline and differential.
GenevisibleiQ64516. MM.

Family and domain databases

InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, mapping, and functional expression of the mouse X chromosome glycerol kinase gene."
    Huq A.H., Lovell R.S., Sampson M.J., Decker W.K., Dinulos M.B., Disteche C.M., Craigen W.J.
    Genomics 36:530-534(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/cJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Retina and Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney and Liver.

Entry informationi

Entry nameiGLPK_MOUSE
AccessioniPrimary (citable) accession number: Q64516
Secondary accession number(s): B1ASZ1, Q8C2M1, Q8C8X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 22, 2008
Last modified: July 6, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.