Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tripeptidyl-peptidase 2

Gene

Tpp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited (By similarity). Stimulates adipogenesis.By similarity1 Publication

Miscellaneous

The limitation of proteolytic products to tripeptides is achieved by tailoring the size of the substrate-binding cleft: the two negatively charged residues Glu-305 and Glu-331 that are blocking position P4 limit the number of residues that can be accommodated in the binding cleft and thus create a molecular ruler. At the same time, they orient substrates so that the tripeptides are removed exclusively from the N-terminus (By similarity).By similarity

Catalytic activityi

Release of an N-terminal tripeptide from a polypeptide.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Charge relay systemBy similarity1
Active sitei264Charge relay systemBy similarity1
Active sitei449Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.14.10 3474
ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiS08.A56

Names & Taxonomyi

Protein namesi
Recommended name:
Tripeptidyl-peptidase 2 (EC:3.4.14.10)
Short name:
TPP-2
Alternative name(s):
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase II
Short name:
TPP-II
Gene namesi
Name:Tpp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:102724 Tpp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Homozygous mutant mice die in utero before embryonic day 9.0. Heterozygous mice display normal food intake but appear lean with a significant reduction in body fat, smaller adipocytes, decreased plasma insulin levels and less white adipose tissue in the gonad, groin and mesenteric regions.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44D → A: No effect on adipogenesis. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5512

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000764232 – 1262Tripeptidyl-peptidase 2Add BLAST1261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei401N6-acetyllysineCombined sources1
Modified residuei915PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ64514
PaxDbiQ64514
PeptideAtlasiQ64514
PRIDEiQ64514

PTM databases

iPTMnetiQ64514
PhosphoSitePlusiQ64514
SwissPalmiQ64514

Expressioni

Tissue specificityi

Expressed in the brain, skeletal muscle, gonadal and mesenteric white adipose tissue and brown adipose tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000041763
CleanExiMM_TPP2
ExpressionAtlasiQ64514 baseline and differential
GenevisibleiQ64514 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204295, 2 interactors
CORUMiQ64514
IntActiQ64514, 2 interactors
MINTiQ64514
STRINGi10090.ENSMUSP00000085244

Structurei

3D structure databases

ProteinModelPortaliQ64514
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 509Peptidase S8Add BLAST470

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Phylogenomic databases

eggNOGiKOG1114 Eukaryota
COG1404 LUCA
GeneTreeiENSGT00390000014623
HOGENOMiHOG000008178
HOVERGENiHBG017992
InParanoidiQ64514
KOiK01280
OMAiWRACIDT
OrthoDBiEOG091G00P0
PhylomeDBiQ64514
TreeFamiTF105647

Family and domain databases

CDDicd04857 Peptidases_S8_Tripeptidyl_Amin, 1 hit
Gene3Di3.40.50.200, 2 hits
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR022229 Peptidase_S8A_TPPII
IPR034051 TPP_II_domain
PfamiView protein in Pfam
PF00082 Peptidase_S8, 1 hit
PF12580 TPPII, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 2 hits
PROSITEiView protein in PROSITE
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q64514-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAATEEPF PFHGLLPKKE TGASSFLCRY PEYDGRGVLI AVLDTGVDPG
60 70 80 90 100
APGMQVTTDG KPKIIDIIDT TGSGDVNTAT EVEPKDGEII GLSGRVLKIP
110 120 130 140 150
ANWTNPLGKY HIGIKNGYDF YPKALKERIQ KERKEKIWDP IHRVALAEAC
160 170 180 190 200
RKQEEFDIAN NGSSQANKLI KEELQSQVEL LNSFEKKYSD PGPVYDCLVW
210 220 230 240 250
HDGETWRACV DSNENGDLSK CAVLRNYKEA QEYSSFGTAE MLNYSVNIYD
260 270 280 290 300
DGNLLSIVTS GGAHGTHVAS IAAGHFPEEP ERNGVAPGAQ ILSIKIGDTR
310 320 330 340 350
LSTMETGTGL IRAMIEVINH KCDLVNYSYG EATHWPNSGR ICEVINEAVW
360 370 380 390 400
KHNTIYVSSA GNNGPCLSTV GCPGGTTSSV IGVGAYVSPD MMVAEYSLRE
410 420 430 440 450
KLPANQYTWS SRGPSADGAL GVSISAPGGA IASVPNWTLR GTQLMNGTSM
460 470 480 490 500
SSPNACGGIA LVLSGLKANN VDYTVHSVRR ALENTAIKAD NIEVFAQGHG
510 520 530 540 550
IIQVDKAYDY LIQNTSFANR LGFTVTVGNN RGIYLRDPVQ VAAPSDHGVG
560 570 580 590 600
IEPVFPENTE NSEKISFQLH LALTSNSSWV QCPSHLELMN QCRHINIRVD
610 620 630 640 650
PRGLREGLHY TEVCGYDIAS PNAGPLFRVP ITAVIAAKVN ESSHYDLAFT
660 670 680 690 700
DVHFKPGQIR RHFVEVPEGA TWAEVTVCSC SSEVSAKFVL HAVQLVKQRA
710 720 730 740 750
YRSHEFYKFC SLPEKGTLIE AFPVLGGKAI EFCIARWWAS LSDVNIDYTI
760 770 780 790 800
SFHGIVCTAP QLNIHASEGI NRFDVQSSLK YEDLAPCITL KSWVQTLRPV
810 820 830 840 850
NAKTRPLGSR DVLPNNRQLY EMVLTYSFHQ PKSGEVTPSC PLLCELLYES
860 870 880 890 900
EFDSQLWIIF DQNKRQMGSG DAYPHQYSLK LEKGDYTIRL QIRHEQISDL
910 920 930 940 950
DRLKDLPFIV SHRLSNTLSL DIHENHSLAL LGKKKSSSLT LPPKYNQPFF
960 970 980 990 1000
VTSLPDDKIP KGAGPGCYLA GSLTLSKTEL GKKAGQSAAK RQGKFKKDVI
1010 1020 1030 1040 1050
PVHYYLIPPP TKIKNGSKDK EKDSEKEKDL KEEFTEALRD LKIQWMTKLD
1060 1070 1080 1090 1100
STDIYNELKE TYPAYLPLYV ARLHQLDAEK ERMKRLNEIV DAANAVISHI
1110 1120 1130 1140 1150
DQTALAVYIA MKTDPRPDAA TIKNDMDKQK STLIDALCRK GCALADHLLH
1160 1170 1180 1190 1200
TQPHDGAAAG DAEAKEEEGE STMESLSETY WETTKWTDLF DTKVLIFAYK
1210 1220 1230 1240 1250
HALVNKMYGR GLKFATKLVE EKPTKENWKN CIQLMKLLGW THCASFTENW
1260
LPIMYPPDYC VF
Length:1,262
Mass (Da):139,879
Last modified:January 23, 2007 - v3
Checksum:iD50D22C85544B034
GO
Isoform Short (identifier: Q64514-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     985-997: Missing.

Show »
Length:1,249
Mass (Da):138,463
Checksum:iA4E59C8D6416EF88
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005446985 – 997Missing in isoform Short. 2 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81323 mRNA Translation: CAA57103.1
BC058239 mRNA Translation: AAH58239.1
CCDSiCCDS14926.1 [Q64514-1]
CCDS78577.1 [Q64514-2]
PIRiI48855
RefSeqiNP_001297469.1, NM_001310540.1 [Q64514-2]
NP_033444.1, NM_009418.3 [Q64514-1]
UniGeneiMm.401675

Genome annotation databases

EnsembliENSMUST00000087933; ENSMUSP00000085244; ENSMUSG00000041763 [Q64514-1]
ENSMUST00000188313; ENSMUSP00000139918; ENSMUSG00000041763 [Q64514-2]
GeneIDi22019
KEGGimmu:22019
UCSCiuc007avs.1 mouse [Q64514-1]
uc007avt.1 mouse [Q64514-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTPP2_MOUSE
AccessioniPrimary (citable) accession number: Q64514
Secondary accession number(s): Q5D072
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health