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Protein

DNA topoisomerase 2-beta

Gene

Top2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.By similarity

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

Cofactori

Mg2+PROSITE-ProRule annotation, Mn2+PROSITE-ProRule annotation, Ca2+PROSITE-ProRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPBy similarity1
Binding sitei129ATPBy similarity1
Metal bindingi470Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi550Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi550Magnesium 2PROSITE-ProRule annotation1
Metal bindingi552Magnesium 2PROSITE-ProRule annotation1
Sitei813Transition state stabilizerBy similarity1
Active sitei814O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1
Sitei865Important for DNA bending; intercalates between base pairs of target DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi157 – 159ATPBy similarity3
Nucleotide bindingi170 – 177ATPBy similarity8
Nucleotide bindingi385 – 387ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • axonogenesis Source: MGI
  • DNA topological change Source: UniProtKB
  • DNA unwinding involved in DNA replication Source: GO_Central
  • forebrain development Source: MGI
  • mitotic DNA integrity checkpoint Source: GO_Central
  • mitotic recombination Source: GO_Central
  • neuron migration Source: MGI
  • resolution of meiotic recombination intermediates Source: GO_Central
  • sister chromatid segregation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-4615885. SUMOylation of DNA replication proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2-beta (EC:5.99.1.3)
Alternative name(s):
DNA topoisomerase II, beta isozyme
Gene namesi
Name:Top2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:98791. Top2b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5564.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001453702 – 1612DNA topoisomerase 2-betaAdd BLAST1611

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei3N6-acetyllysineCombined sources1
Cross-linki22Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki165Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki361Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki425Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki593Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki631Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1215Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1224PhosphoserineBy similarity1
Cross-linki1238Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1259Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1280PhosphothreonineBy similarity1
Modified residuei1324PhosphoserineCombined sources1
Modified residuei1328PhosphoserineBy similarity1
Modified residuei1330PhosphoserineBy similarity1
Modified residuei1332PhosphoserineBy similarity1
Modified residuei1346PhosphoserineBy similarity1
Modified residuei1358PhosphotyrosineCombined sources1
Modified residuei1363PhosphoserineCombined sources1
Modified residuei1376PhosphoserineCombined sources1
Modified residuei1387PhosphoserineCombined sources1
Modified residuei1390PhosphothreonineCombined sources1
Modified residuei1400PhosphoserineCombined sources1
Modified residuei1408PhosphotyrosineCombined sources1
Modified residuei1411PhosphoserineBy similarity1
Cross-linki1427Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1428PhosphoserineBy similarity1
Modified residuei1439PhosphoserineBy similarity1
Modified residuei1441PhosphoserineBy similarity1
Cross-linki1443Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1448PhosphoserineBy similarity1
Modified residuei1453PhosphoserineCombined sources1
Modified residuei1460PhosphoserineCombined sources1
Modified residuei1509PhosphoserineCombined sources1
Modified residuei1511PhosphoserineCombined sources1
Modified residuei1513PhosphoserineCombined sources1
Modified residuei1537PhosphoserineCombined sources1
Modified residuei1539PhosphoserineCombined sources1
Modified residuei1562PhosphothreonineBy similarity1
Modified residuei1563PhosphoserineBy similarity1
Modified residuei1568PhosphoserineCombined sources1
Modified residuei1596PhosphotyrosineBy similarity1
Modified residuei1600PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ64511.
MaxQBiQ64511.
PaxDbiQ64511.
PeptideAtlasiQ64511.
PRIDEiQ64511.

PTM databases

iPTMnetiQ64511.
PhosphoSitePlusiQ64511.

Expressioni

Gene expression databases

BgeeiENSMUSG00000017485.
CleanExiMM_TOP2B.
ExpressionAtlasiQ64511. baseline and differential.
GenevisibleiQ64511. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei498Interaction with DNAPROSITE-ProRule annotation1
Sitei501Interaction with DNAPROSITE-ProRule annotation1
Sitei670Interaction with DNAPROSITE-ProRule annotation1
Sitei671Interaction with DNAPROSITE-ProRule annotation1
Sitei732Interaction with DNAPROSITE-ProRule annotation1
Sitei766Interaction with DNAPROSITE-ProRule annotation1
Sitei940Interaction with DNAPROSITE-ProRule annotation1

Binary interactionsi

WithEntry#Exp.IntActNotes
AicdaQ9WVE03EBI-2325586,EBI-3835567

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204277. 8 interactors.
IntActiQ64511. 7 interactors.
MINTiMINT-4138251.
STRINGi10090.ENSMUSP00000017629.

Structurei

3D structure databases

ProteinModelPortaliQ64511.
SMRiQ64511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini464 – 581ToprimPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni351 – 353Interaction with DNABy similarity3
Regioni999 – 1008Interaction with DNABy similarity10

Sequence similaritiesi

Belongs to the type II topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0355. Eukaryota.
COG0187. LUCA.
COG0188. LUCA.
GeneTreeiENSGT00390000016222.
HOGENOMiHOG000216693.
HOVERGENiHBG052998.
InParanoidiQ64511.
KOiK03164.
OMAiEETQNQH.
OrthoDBiEOG091G00U2.
PhylomeDBiQ64511.
TreeFamiTF105282.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR012542. DTHCT.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR028467. Top2b.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR031660. TOPRIM_C.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR10169:SF36. PTHR10169:SF36. 1 hit.
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF08070. DTHCT. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
PF16898. TOPRIM_C. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSSLAGSD GALTWVNNAT KKEELETANK NDSTKKLSVE RVYQKKTQLE
60 70 80 90 100
HILLRPDTYI GSVEPLTQLM WVYDEDVGMN CREVTFVPGL YKIFDEILVN
110 120 130 140 150
AADNKQRDKN MTCIKVSIDP ESNIISIWNN GKGIPVVEHK VEKVYVPALI
160 170 180 190 200
FGQLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTKFTVET ACKEYKHSFK
210 220 230 240 250
QTWMNNMMKT SEAKIKHFDG EDYTCITFQP DLSKFKMEKL DKDIVALMTR
260 270 280 290 300
RAYDLAGSCK GVKVMFNGKK LPVNGFRSYV DLYVKDKLDE TGVALKVIHE
310 320 330 340 350
LANERWDVCL TLSEKGFQQI SFVNSIATTK GGRHVDYVVD QVVSKLIEVV
360 370 380 390 400
KKKNKAGVSV KPFQVKNHIW VFINCLIENP TFDSQTKENM TLQPKSFGSK
410 420 430 440 450
CQLSEKFFKA ASNCGIVESI LNWVKFKAQT QLNKKCSSVK YSKIKGIPKL
460 470 480 490 500
DDANDAGGKH SLECTLILTE GDSAKSLAVS GLGVIGRDRY GVFPLRGKIL
510 520 530 540 550
NVREASHKQI MENAEINNII KIVGLQYKKS YDDAESLKTL RYGKIMIMTD
560 570 580 590 600
QDQDGSHIKG LLINFIHHNW PSLLKHGFLE EFITPIVKAS KNKQELSFYS
610 620 630 640 650
IPEFDEWKKH IENQKAWKIK YYKGLGTSTA KEAKEYFADM ERHRILFRYA
660 670 680 690 700
GPEDDAAITL AFSKKKIDDR KEWLTNFMED RRQRRLHGLP EQFLYGTATK
710 720 730 740 750
HLTYNDFINK ELILFSNSDN ERSIPSLVDG FKPGQRKVLF TCFKRNDKRE
760 770 780 790 800
VKVAQLAGSV AEMSAYHHGE QALMMTIVNL AQNFVGSNNI NLLQPIGQFG
810 820 830 840 850
TRLHGGKDAA SPRYIFTMLS SLARLLFPAV DDNLLKFLYD DNQRVEPEWY
860 870 880 890 900
IPIIPMVLIN GAEGIGTGWA CKLPNYDARE IVNNVRRMLE GLDPHPMLPN
910 920 930 940 950
YKNFKGTIQE LGQNQYAVSG EIFVVDRNTV EITELPVRTW TQVYKEQVLE
960 970 980 990 1000
PMLNGTDKTP ALISDYKEYH TDTTVKFVVK MTEEKLAQAE AAGLHKVFKL
1010 1020 1030 1040 1050
QTTLTCNSMV LFDHMGCLKK YETVQDILKE FFDLRLSYYG LRKEWLVGML
1060 1070 1080 1090 1100
GAESTKLNNQ ARFILEKIQG KITIENRSKK DLIQMLVQRG YESDPVKAWK
1110 1120 1130 1140 1150
EAQEKAAEEE DSQNQHDDSS SDSGTPSGPD FNYILNMSLW SLTKEKVEEL
1160 1170 1180 1190 1200
IKQRDTKGRE VNDLKRKSPS DLWKEDLAAF VEELDKVEAQ EREDILAGMS
1210 1220 1230 1240 1250
GKAIKGKVGK PKVKKLQLEE TMPSPYGRRI VPEITAMKAD ASRKLLKKKK
1260 1270 1280 1290 1300
GDPDTTVVKV EFDEEFSGTP AEGTGEETLT PSAPVNKGPK PKREKKEPGT
1310 1320 1330 1340 1350
RVRKTPTSTG KTNAKKVKKR NPWSDDESKS ESDLEEAEPV VIPRDSLLRR
1360 1370 1380 1390 1400
AAAERPKYTF DFSEEEDDDA AAADDSNDLE ELKVKASPIT NDGEDEFVPS
1410 1420 1430 1440 1450
DGLDKDEYAF SSGKSKATPE KSSNDKKSQD FGNLFSFPSY SQKSEDDSAK
1460 1470 1480 1490 1500
FDSNEEDTAS VFAPSFGLKQ TDKLPSKTVA AKKGKPPSDT APKAKRAPKQ
1510 1520 1530 1540 1550
KKIVETINSD SDSEFGIPKK TTTPKGKGRG AKKRKASGSE NEGDYNPGRK
1560 1570 1580 1590 1600
PSKTASKKPK KTSFDQDSDV DIFPSDFTSE PPALPRTGRA RKEVKYFAES
1610
DEEEDVDFAM FN
Length:1,612
Mass (Da):181,909
Last modified:September 27, 2005 - v2
Checksum:i974D9D5DAD0DB96A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16V → A in BAA07236 (Ref. 1) Curated1
Sequence conflicti1434L → P in BAA07236 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38046 mRNA. Translation: BAA07236.1.
BC041106 mRNA. Translation: AAH41106.1.
BC054541 mRNA. Translation: AAH54541.1.
CCDSiCCDS26833.1.
RefSeqiNP_033435.2. NM_009409.2.
UniGeneiMm.130362.

Genome annotation databases

EnsembliENSMUST00000017629; ENSMUSP00000017629; ENSMUSG00000017485.
GeneIDi21974.
KEGGimmu:21974.
UCSCiuc007shc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38046 mRNA. Translation: BAA07236.1.
BC041106 mRNA. Translation: AAH41106.1.
BC054541 mRNA. Translation: AAH54541.1.
CCDSiCCDS26833.1.
RefSeqiNP_033435.2. NM_009409.2.
UniGeneiMm.130362.

3D structure databases

ProteinModelPortaliQ64511.
SMRiQ64511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204277. 8 interactors.
IntActiQ64511. 7 interactors.
MINTiMINT-4138251.
STRINGi10090.ENSMUSP00000017629.

Chemistry databases

ChEMBLiCHEMBL5564.

PTM databases

iPTMnetiQ64511.
PhosphoSitePlusiQ64511.

Proteomic databases

EPDiQ64511.
MaxQBiQ64511.
PaxDbiQ64511.
PeptideAtlasiQ64511.
PRIDEiQ64511.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017629; ENSMUSP00000017629; ENSMUSG00000017485.
GeneIDi21974.
KEGGimmu:21974.
UCSCiuc007shc.1. mouse.

Organism-specific databases

CTDi7155.
MGIiMGI:98791. Top2b.

Phylogenomic databases

eggNOGiKOG0355. Eukaryota.
COG0187. LUCA.
COG0188. LUCA.
GeneTreeiENSGT00390000016222.
HOGENOMiHOG000216693.
HOVERGENiHBG052998.
InParanoidiQ64511.
KOiK03164.
OMAiEETQNQH.
OrthoDBiEOG091G00U2.
PhylomeDBiQ64511.
TreeFamiTF105282.

Enzyme and pathway databases

ReactomeiR-MMU-4615885. SUMOylation of DNA replication proteins.

Miscellaneous databases

PROiQ64511.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017485.
CleanExiMM_TOP2B.
ExpressionAtlasiQ64511. baseline and differential.
GenevisibleiQ64511. MM.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR012542. DTHCT.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR028467. Top2b.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR031660. TOPRIM_C.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR10169:SF36. PTHR10169:SF36. 1 hit.
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF08070. DTHCT. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
PF16898. TOPRIM_C. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP2B_MOUSE
AccessioniPrimary (citable) accession number: Q64511
Secondary accession number(s): Q7TQG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 27, 2005
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.