Reviewed, UniProtKB/Swiss-Prot Q64505 (CP7A1_MOUSE)

Names and origin

Protein names

Recommended name:
    Cholesterol 7-alpha-monooxygenase
    EC=1.14.13.17
Alternative name(s):
    Cytochrome P450 7A1
    CYPVII
    Cholesterol 7-alpha-hydroxylase

Gene names

Name:	Cyp7a1
Synonyms:	Cyp7

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
Catalytic activity	Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; bile acid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	Up-regulated by fasting, returns to ground state upon feeding. Up-regulated by experimentally induced diabetes. Down-regulated by insulin treatment.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	cholesterol catabolic process Inferred from direct assay. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of bile acid biosynthetic process Traceable author statement. Source: UniProtKB
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: MGI
Molecular function	cholesterol 7-alpha-monooxygenase activity Inferred from direct assay. Source: MGI electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Cholesterol 7-alpha-monooxygenase		PRO_0000051902
Sites
	Metal binding	444	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64505-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 51DB3C71D6C79791	FASTA	503	57,258
	10 20 30 40 50 60 MMSISLIWGI AVVVSCCIWF IIGIRRRKVG EPPLDNGLIP YLGCALKFGS NPLEFLRAKQ 70 80 90 100 110 120 RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HYTTSAKAFG HRSIDPSDGN 130 140 150 160 170 180 TTENINKTFN KTLQGDALCS LSEAMMQNLQ SVMRPPGLPK SKSAVWVTEG MYAFCYRVMF 190 200 210 220 230 240 EAGYLTLFGK DISKTDTQRA FIQNNLDSFK QFDQVFPALV AGVPIHLLKT AHKARERLAE 250 260 270 280 290 300 SLKHKNLYMR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL VILWASQANT IPATFWSLFQ 310 320 330 340 350 360 MIRSPEAMKA ASEEVNGSLQ SAGQELSSGG NAIYLDQEQL NNLPVLDSII KEALRLSSAS 370 380 390 400 410 420 LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAK 430 440 450 460 470 480 TTFYRNGNKL KYFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSYFELELV ESHTKCPPLD 490 500 QSRAGLGILP PLNDIEFKYK LKH

References

[1]	"Structure of the mouse cholesterol 7 alpha-hydroxylase gene." Tzung K.W., Ishimura-Oka K., Kihara S., Oka K., Chan L. Genomics 21:244-247(1994) [PubMed: 8088795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"PGC-1alpha activates CYP7A1 and bile acid biosynthesis." Shin D.J., Campos J.A., Gil G., Osborne T.F. J. Biol. Chem. 278:50047-50052(2003) [PubMed: 14522988] [Abstract] Cited for: FUNCTION, INDUCTION BY FASTING.
[3]	"Functional interaction of hepatic nuclear factor-4 and peroxisome proliferator-activated receptor-gamma coactivator 1alpha in CYP7A1 regulation is inhibited by a key lipogenic activator, sterol regulatory element-binding protein-1c." Ponugoti B., Fang S., Kemper J.K. Mol. Endocrinol. 21:2698-2712(2007) [PubMed: 17636037] [Abstract] Cited for: INDUCTION BY FASTING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23754 Genomic DNA. Translation: AAA68867.1.
IPI	IPI00135398.
PIR	A54779.
UniGene	Mm.57029
3D structure databases
SMR	Q64505. Positions 25-502.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64505.
Proteomic databases
PRIDE	Q64505.
Genome annotation databases
Ensembl	ENSMUST00000029905; ENSMUSP00000029905; ENSMUSG00000028240; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:106091. Cyp7a1.
Phylogenomic databases
HOGENOM	HBG446017.
HOVERGEN	Q64505.
InParanoid	Q64505.
PhylomeDB	Q64505.
Enzyme and pathway databases
BRENDA	1.14.13.17. 244.
Gene expression databases
ArrayExpress	Q64505.
Bgee	Q64505.
Genevestigator	Q64505.
GermOnline	ENSMUSG00000028240. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CP7A1_MOUSE

Accession

Primary (citable) accession number: Q64505

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)