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Protein

Cholesterol 7-alpha-monooxygenase

Gene

Cyp7a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.1 Publication

Catalytic activityi

Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi444 – 4441Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. cholesterol 7-alpha-monooxygenase activity Source: UniProtKB
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. bile acid biosynthetic process Source: UniProtKB
  2. cellular response to cholesterol Source: UniProtKB
  3. cellular response to glucose stimulus Source: UniProtKB
  4. cholesterol catabolic process Source: UniProtKB
  5. cholesterol homeostasis Source: UniProtKB
  6. positive regulation of bile acid biosynthetic process Source: BHF-UCL
  7. regulation of bile acid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.17. 3474.
ReactomeiREACT_272139. PPARA activates gene expression.
REACT_281686. Endogenous sterols.
REACT_297274. Orphan transporters.
REACT_325766. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
UniPathwayiUPA00221.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 7-alpha-monooxygenase (EC:1.14.13.17)
Alternative name(s):
CYPVII
Cholesterol 7-alpha-hydroxylase
Cytochrome P450 7A1
Gene namesi
Name:Cyp7a1
Synonyms:Cyp7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:106091. Cyp7a1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. intracellular membrane-bounded organelle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Cholesterol 7-alpha-monooxygenasePRO_0000051902Add
BLAST

Proteomic databases

MaxQBiQ64505.
PaxDbiQ64505.
PRIDEiQ64505.

PTM databases

PhosphoSiteiQ64505.

Expressioni

Inductioni

Up-regulated by fasting, returns to ground state upon feeding. Up-regulated by experimentally induced diabetes. Down-regulated by insulin treatment.2 Publications

Gene expression databases

BgeeiQ64505.
GenevestigatoriQ64505.

Structurei

3D structure databases

ProteinModelPortaliQ64505.
SMRiQ64505. Positions 25-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ64505.
KOiK00489.
OMAiFHYTTSA.
OrthoDBiEOG7J9VP6.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030681. Cholesterol_7a_monooxygenase.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERiPTHR24304:SF1. PTHR24304:SF1. 1 hit.
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500625. Cytochrome_CYP7A1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSISLIWGI AVVVSCCIWF IIGIRRRKVG EPPLDNGLIP YLGCALKFGS
60 70 80 90 100
NPLEFLRAKQ RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF
110 120 130 140 150
HYTTSAKAFG HRSIDPSDGN TTENINKTFN KTLQGDALCS LSEAMMQNLQ
160 170 180 190 200
SVMRPPGLPK SKSAVWVTEG MYAFCYRVMF EAGYLTLFGK DISKTDSQRA
210 220 230 240 250
FIQNNLDSFK QFDQVFPALV AGVPIHLFKT AHKARERLAE SLKHKNLYMR
260 270 280 290 300
DQVSELIRLR MFLNDTLSTF DDMEKAKTHL VILWASQANT IPATFWSLFQ
310 320 330 340 350
MIRSPEAMKA ASEEVNGALQ SAGQELSSGG NAIYLDQEQL NNLPVLDSII
360 370 380 390 400
KEALRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI
410 420 430 440 450
YPDPLTFKYD RYLDESGKAK TTFYRNGNKL KYFYMPFGSG ATICPGRLFA
460 470 480 490 500
VQEIKQFLIL MLSYFELELV ESHTKCPPLD QSRAGLGILP PLNDIEFKYK

LKH
Length:503
Mass (Da):57,262
Last modified:July 26, 2011 - v2
Checksum:iF7F8BC2CDD2C43D1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971S → T in AAA68867 (PubMed:8088795).Curated
Sequence conflicti228 – 2281F → L in AAA68867 (PubMed:8088795).Curated
Sequence conflicti318 – 3181A → S in AAA68867 (PubMed:8088795).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23754 Genomic DNA. Translation: AAA68867.1.
AK050020 mRNA. Translation: BAC34033.1.
AK050210 mRNA. Translation: BAC34123.1.
AK050220 mRNA. Translation: BAC34131.1.
AK050260 mRNA. Translation: BAC34150.1.
AL772306 Genomic DNA. Translation: CAM27235.1.
CCDSiCCDS17950.1.
PIRiA54779.
RefSeqiNP_031850.2. NM_007824.2.
XP_006537666.1. XM_006537603.1.
UniGeneiMm.57029.

Genome annotation databases

EnsembliENSMUST00000029905; ENSMUSP00000029905; ENSMUSG00000028240.
GeneIDi13122.
KEGGimmu:13122.
UCSCiuc008rxk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23754 Genomic DNA. Translation: AAA68867.1.
AK050020 mRNA. Translation: BAC34033.1.
AK050210 mRNA. Translation: BAC34123.1.
AK050220 mRNA. Translation: BAC34131.1.
AK050260 mRNA. Translation: BAC34150.1.
AL772306 Genomic DNA. Translation: CAM27235.1.
CCDSiCCDS17950.1.
PIRiA54779.
RefSeqiNP_031850.2. NM_007824.2.
XP_006537666.1. XM_006537603.1.
UniGeneiMm.57029.

3D structure databases

ProteinModelPortaliQ64505.
SMRiQ64505. Positions 25-503.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2212.

PTM databases

PhosphoSiteiQ64505.

Proteomic databases

MaxQBiQ64505.
PaxDbiQ64505.
PRIDEiQ64505.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029905; ENSMUSP00000029905; ENSMUSG00000028240.
GeneIDi13122.
KEGGimmu:13122.
UCSCiuc008rxk.1. mouse.

Organism-specific databases

CTDi1581.
MGIiMGI:106091. Cyp7a1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ64505.
KOiK00489.
OMAiFHYTTSA.
OrthoDBiEOG7J9VP6.
TreeFamiTF105090.

Enzyme and pathway databases

UniPathwayiUPA00221.
BRENDAi1.14.13.17. 3474.
ReactomeiREACT_272139. PPARA activates gene expression.
REACT_281686. Endogenous sterols.
REACT_297274. Orphan transporters.
REACT_325766. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.

Miscellaneous databases

ChiTaRSiCyp7a1. mouse.
NextBioi283162.
PROiQ64505.
SOURCEiSearch...

Gene expression databases

BgeeiQ64505.
GenevestigatoriQ64505.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030681. Cholesterol_7a_monooxygenase.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERiPTHR24304:SF1. PTHR24304:SF1. 1 hit.
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500625. Cytochrome_CYP7A1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the mouse cholesterol 7 alpha-hydroxylase gene."
    Tzung K.W., Ishimura-Oka K., Kihara S., Oka K., Chan L.
    Genomics 21:244-247(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "PGC-1alpha activates CYP7A1 and bile acid biosynthesis."
    Shin D.J., Campos J.A., Gil G., Osborne T.F.
    J. Biol. Chem. 278:50047-50052(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY FASTING.
  5. "Functional interaction of hepatic nuclear factor-4 and peroxisome proliferator-activated receptor-gamma coactivator 1alpha in CYP7A1 regulation is inhibited by a key lipogenic activator, sterol regulatory element-binding protein-1c."
    Ponugoti B., Fang S., Kemper J.K.
    Mol. Endocrinol. 21:2698-2712(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY FASTING.

Entry informationi

Entry nameiCP7A1_MOUSE
AccessioniPrimary (citable) accession number: Q64505
Secondary accession number(s): Q8BFR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1999
Last sequence update: July 26, 2011
Last modified: March 31, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.