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Q64505

- CP7A1_MOUSE

UniProt

Q64505 - CP7A1_MOUSE

Protein

Cholesterol 7-alpha-monooxygenase

Gene

Cyp7a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.1 Publication

    Catalytic activityi

    Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi444 – 4441Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. cholesterol 7-alpha-monooxygenase activity Source: UniProtKB
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. bile acid biosynthetic process Source: UniProtKB
    2. cellular response to cholesterol Source: UniProtKB
    3. cellular response to glucose stimulus Source: UniProtKB
    4. cholesterol catabolic process Source: UniProtKB
    5. cholesterol homeostasis Source: UniProtKB
    6. positive regulation of bile acid biosynthetic process Source: BHF-UCL
    7. regulation of bile acid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.
    REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    REACT_227038. Endogenous sterols.
    UniPathwayiUPA00221.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholesterol 7-alpha-monooxygenase (EC:1.14.13.17)
    Alternative name(s):
    CYPVII
    Cholesterol 7-alpha-hydroxylase
    Cytochrome P450 7A1
    Gene namesi
    Name:Cyp7a1
    Synonyms:Cyp7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:106091. Cyp7a1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 503503Cholesterol 7-alpha-monooxygenasePRO_0000051902Add
    BLAST

    Proteomic databases

    MaxQBiQ64505.
    PaxDbiQ64505.
    PRIDEiQ64505.

    PTM databases

    PhosphoSiteiQ64505.

    Expressioni

    Inductioni

    Up-regulated by fasting, returns to ground state upon feeding. Up-regulated by experimentally induced diabetes. Down-regulated by insulin treatment.2 Publications

    Gene expression databases

    BgeeiQ64505.
    GenevestigatoriQ64505.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64505.
    SMRiQ64505. Positions 25-503.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00550000074551.
    HOGENOMiHOG000231026.
    HOVERGENiHBG051100.
    InParanoidiQ8BFR7.
    KOiK00489.
    OMAiFHYTTSA.
    OrthoDBiEOG7J9VP6.
    TreeFamiTF105090.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR024204. Cyt_P450_CYP7A1-type.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
    PRINTSiPR00465. EP450IV.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64505-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMSISLIWGI AVVVSCCIWF IIGIRRRKVG EPPLDNGLIP YLGCALKFGS    50
    NPLEFLRAKQ RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF 100
    HYTTSAKAFG HRSIDPSDGN TTENINKTFN KTLQGDALCS LSEAMMQNLQ 150
    SVMRPPGLPK SKSAVWVTEG MYAFCYRVMF EAGYLTLFGK DISKTDSQRA 200
    FIQNNLDSFK QFDQVFPALV AGVPIHLFKT AHKARERLAE SLKHKNLYMR 250
    DQVSELIRLR MFLNDTLSTF DDMEKAKTHL VILWASQANT IPATFWSLFQ 300
    MIRSPEAMKA ASEEVNGALQ SAGQELSSGG NAIYLDQEQL NNLPVLDSII 350
    KEALRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI 400
    YPDPLTFKYD RYLDESGKAK TTFYRNGNKL KYFYMPFGSG ATICPGRLFA 450
    VQEIKQFLIL MLSYFELELV ESHTKCPPLD QSRAGLGILP PLNDIEFKYK 500
    LKH 503
    Length:503
    Mass (Da):57,262
    Last modified:July 27, 2011 - v2
    Checksum:iF7F8BC2CDD2C43D1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti197 – 1971S → T in AAA68867. (PubMed:8088795)Curated
    Sequence conflicti228 – 2281F → L in AAA68867. (PubMed:8088795)Curated
    Sequence conflicti318 – 3181A → S in AAA68867. (PubMed:8088795)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23754 Genomic DNA. Translation: AAA68867.1.
    AK050020 mRNA. Translation: BAC34033.1.
    AK050210 mRNA. Translation: BAC34123.1.
    AK050220 mRNA. Translation: BAC34131.1.
    AK050260 mRNA. Translation: BAC34150.1.
    AL772306 Genomic DNA. Translation: CAM27235.1.
    CCDSiCCDS17950.1.
    PIRiA54779.
    RefSeqiNP_031850.2. NM_007824.2.
    XP_006537666.1. XM_006537603.1.
    UniGeneiMm.57029.

    Genome annotation databases

    EnsembliENSMUST00000029905; ENSMUSP00000029905; ENSMUSG00000028240.
    GeneIDi13122.
    KEGGimmu:13122.
    UCSCiuc008rxk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23754 Genomic DNA. Translation: AAA68867.1 .
    AK050020 mRNA. Translation: BAC34033.1 .
    AK050210 mRNA. Translation: BAC34123.1 .
    AK050220 mRNA. Translation: BAC34131.1 .
    AK050260 mRNA. Translation: BAC34150.1 .
    AL772306 Genomic DNA. Translation: CAM27235.1 .
    CCDSi CCDS17950.1.
    PIRi A54779.
    RefSeqi NP_031850.2. NM_007824.2.
    XP_006537666.1. XM_006537603.1.
    UniGenei Mm.57029.

    3D structure databases

    ProteinModelPortali Q64505.
    SMRi Q64505. Positions 25-503.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL2212.

    PTM databases

    PhosphoSitei Q64505.

    Proteomic databases

    MaxQBi Q64505.
    PaxDbi Q64505.
    PRIDEi Q64505.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029905 ; ENSMUSP00000029905 ; ENSMUSG00000028240 .
    GeneIDi 13122.
    KEGGi mmu:13122.
    UCSCi uc008rxk.1. mouse.

    Organism-specific databases

    CTDi 1581.
    MGIi MGI:106091. Cyp7a1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00550000074551.
    HOGENOMi HOG000231026.
    HOVERGENi HBG051100.
    InParanoidi Q8BFR7.
    KOi K00489.
    OMAi FHYTTSA.
    OrthoDBi EOG7J9VP6.
    TreeFami TF105090.

    Enzyme and pathway databases

    UniPathwayi UPA00221 .
    Reactomei REACT_198602. PPARA activates gene expression.
    REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    REACT_227038. Endogenous sterols.

    Miscellaneous databases

    NextBioi 283162.
    PROi Q64505.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q64505.
    Genevestigatori Q64505.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR024204. Cyt_P450_CYP7A1-type.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
    PRINTSi PR00465. EP450IV.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the mouse cholesterol 7 alpha-hydroxylase gene."
      Tzung K.W., Ishimura-Oka K., Kihara S., Oka K., Chan L.
      Genomics 21:244-247(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Liver.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "PGC-1alpha activates CYP7A1 and bile acid biosynthesis."
      Shin D.J., Campos J.A., Gil G., Osborne T.F.
      J. Biol. Chem. 278:50047-50052(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY FASTING.
    5. "Functional interaction of hepatic nuclear factor-4 and peroxisome proliferator-activated receptor-gamma coactivator 1alpha in CYP7A1 regulation is inhibited by a key lipogenic activator, sterol regulatory element-binding protein-1c."
      Ponugoti B., Fang S., Kemper J.K.
      Mol. Endocrinol. 21:2698-2712(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY FASTING.

    Entry informationi

    Entry nameiCP7A1_MOUSE
    AccessioniPrimary (citable) accession number: Q64505
    Secondary accession number(s): Q8BFR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3