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Q64471

- GSTT1_MOUSE

UniProt

Q64471 - GSTT1_MOUSE

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Protein

Glutathione S-transferase theta-1

Gene

Gstt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Also binds steroids, bilirubin, carcinogens and numerous organic anions. Has dichloromethane dehalogenase activity.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione peroxidase activity Source: Ensembl
  2. glutathione transferase activity Source: UniProtKB

GO - Biological processi

  1. glutathione metabolic process Source: UniProtKB
  2. response to drug Source: Ensembl
  3. response to organic cyclic compound Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_215316. Glutathione conjugation.
SABIO-RKQ64471.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase theta-1 (EC:2.5.1.18)
Alternative name(s):
GST class-theta-1
Gene namesi
Name:Gstt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:107379. Gstt1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi234 – 2341R → W: Strongly reduced catalytic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 240239Glutathione S-transferase theta-1PRO_0000185940Add
BLAST

Proteomic databases

PaxDbiQ64471.
PRIDEiQ64471.

2D gel databases

SWISS-2DPAGEQ64471.

PTM databases

PhosphoSiteiQ64471.

Expressioni

Tissue specificityi

In liver, highest expression found in central vein limiting plate hepatocytes. Also expressed in interlobular bile duct epithelial cells. In lung, expressed in Clara cells and ciliated cells of the bronchiolar epithelium and in type II alveolar cells of the lung parenchyma.

Gene expression databases

CleanExiMM_GSTT1.
ExpressionAtlasiQ64471. baseline and differential.
GenevestigatoriQ64471.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiQ64471. 2 interactions.
MINTiMINT-1865860.

Structurei

3D structure databases

ProteinModelPortaliQ64471.
SMRiQ64471. Positions 3-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8281GST N-terminalAdd
BLAST
Domaini88 – 222135GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 542Glutathione bindingBy similarity
Regioni66 – 672Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00540000069741.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiQ64471.
KOiK00799.
OMAiRRNCLRT.
OrthoDBiEOG7GTT59.
TreeFamiTF325759.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64471-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLELYLDLL SQPCRAIYIF AKKNNIPFQM HTVELRKGEH LSDAFARVNP
60 70 80 90 100
MKRVPAMMDG GFTLCESVAI LLYLAHKYKV PDHWYPQDLQ ARARVDEYLA
110 120 130 140 150
WQHTGLRRSC LRALWHKVMF PVFLGEQIPP ETLAATLAEL DVNLQVLEDK
160 170 180 190 200
FLQDKDFLVG PHISLADLVA ITELMHPVGG GCPVFEGHPR LAAWYQRVEA
210 220 230 240
AVGKDLFREA HEVILKVKDC PPADLIIKQK LMPRVLAMIQ
Length:240
Mass (Da):27,374
Last modified:October 3, 2012 - v4
Checksum:iEDCB477D557466DB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531R → K in CAA66665. (PubMed:8761485)Curated
Sequence conflicti237 – 2371A → T in CAA66665. (PubMed:8761485)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98055 mRNA. Translation: CAA66665.1.
AC142499 Genomic DNA. No translation available.
CH466553 Genomic DNA. Translation: EDL31888.1.
BC012254 mRNA. Translation: AAH12254.1.
BC055020 mRNA. Translation: AAH55020.1.
CCDSiCCDS23932.1.
PIRiS71878.
RefSeqiNP_032211.3. NM_008185.3.
UniGeneiMm.2746.

Genome annotation databases

EnsembliENSMUST00000001713; ENSMUSP00000001713; ENSMUSG00000001663.
GeneIDi14871.
KEGGimmu:14871.
UCSCiuc007frc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98055 mRNA. Translation: CAA66665.1 .
AC142499 Genomic DNA. No translation available.
CH466553 Genomic DNA. Translation: EDL31888.1 .
BC012254 mRNA. Translation: AAH12254.1 .
BC055020 mRNA. Translation: AAH55020.1 .
CCDSi CCDS23932.1.
PIRi S71878.
RefSeqi NP_032211.3. NM_008185.3.
UniGenei Mm.2746.

3D structure databases

ProteinModelPortali Q64471.
SMRi Q64471. Positions 3-240.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q64471. 2 interactions.
MINTi MINT-1865860.

PTM databases

PhosphoSitei Q64471.

2D gel databases

SWISS-2DPAGE Q64471.

Proteomic databases

PaxDbi Q64471.
PRIDEi Q64471.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001713 ; ENSMUSP00000001713 ; ENSMUSG00000001663 .
GeneIDi 14871.
KEGGi mmu:14871.
UCSCi uc007frc.2. mouse.

Organism-specific databases

CTDi 2952.
MGIi MGI:107379. Gstt1.

Phylogenomic databases

eggNOGi COG0625.
GeneTreei ENSGT00540000069741.
HOGENOMi HOG000125747.
HOVERGENi HBG051854.
InParanoidi Q64471.
KOi K00799.
OMAi RRNCLRT.
OrthoDBi EOG7GTT59.
TreeFami TF325759.

Enzyme and pathway databases

Reactomei REACT_215316. Glutathione conjugation.
SABIO-RK Q64471.

Miscellaneous databases

NextBioi 287137.
PROi Q64471.
SOURCEi Search...

Gene expression databases

CleanExi MM_GSTT1.
ExpressionAtlasi Q64471. baseline and differential.
Genevestigatori Q64471.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human."
    Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T.
    Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X C3H.
    Tissue: Liver and Lung.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver and Salivary gland.
  5. "Residue 234 is a master switch of the alternative-substrate activity profile of human and rodent theta class glutathione transferase T1-1."
    Shokeer A., Mannervik B.
    Biochim. Biophys. Acta 1800:466-473(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ARG-234.

Entry informationi

Entry nameiGSTT1_MOUSE
AccessioniPrimary (citable) accession number: Q64471
Secondary accession number(s): Q91X50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 3, 2012
Last modified: October 29, 2014
This is version 108 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3