Q64471 (GSTT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase theta-1
EC=2.5.1.18

Alternative name(s):
GST class-theta-1

Gene names

Name:

Gstt1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Also binds steroids, bilirubin, carcinogens and numerous organic anions. Has dichloromethane dehalogenase activity. Ref.5
Catalytic activity	RX + glutathione = HX + R-S-glutathione. Ref.5
Subunit structure	Homodimer.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	In liver, highest expression found in central vein limiting plate hepatocytes. Also expressed in interlobular bile duct epithelial cells. In lung, expressed in Clara cells and ciliated cells of the bronchiolar epithelium and in type II alveolar cells of the lung parenchyma.
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from direct assay Ref.5. Source: UniProtKB response to drug Inferred from electronic annotation. Source: Compara response to organic cyclic compound Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione peroxidase activity Inferred from electronic annotation. Source: Compara glutathione transferase activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 240	239	Glutathione S-transferase theta-1		PRO_0000185940
Regions
	Domain	2 – 82	81	GST N-terminal
	Domain	88 – 222	135	GST C-terminal
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Sites
	Binding site	40	1	Glutathione By similarity
Experimental info
	Mutagenesis	234	1	R → W: Strongly reduced catalytic activity. Ref.5
	Sequence conflict	53	1	R → K in CAA66665. Ref.1
	Sequence conflict	237	1	A → T in CAA66665. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q64471 [UniParc]. Last modified October 3, 2012. Version 4. Checksum: EDCB477D557466DB	FASTA	240	27,374
	10 20 30 40 50 60 MVLELYLDLL SQPCRAIYIF AKKNNIPFQM HTVELRKGEH LSDAFARVNP MKRVPAMMDG 70 80 90 100 110 120 GFTLCESVAI LLYLAHKYKV PDHWYPQDLQ ARARVDEYLA WQHTGLRRSC LRALWHKVMF 130 140 150 160 170 180 PVFLGEQIPP ETLAATLAEL DVNLQVLEDK FLQDKDFLVG PHISLADLVA ITELMHPVGG 190 200 210 220 230 240 GCPVFEGHPR LAAWYQRVEA AVGKDLFREA HEVILKVKDC PPADLIIKQK LMPRVLAMIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human." Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T. Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X C3H. Tissue: Liver and Lung.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver and Salivary gland.
[5]	"Residue 234 is a master switch of the alternative-substrate activity profile of human and rodent theta class glutathione transferase T1-1." Shokeer A., Mannervik B. Biochim. Biophys. Acta 1800:466-473(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ARG-234.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98055 mRNA. Translation: CAA66665.1. AC142499 Genomic DNA. No translation available. CH466553 Genomic DNA. Translation: EDL31888.1. BC012254 mRNA. Translation: AAH12254.1. BC055020 mRNA. Translation: AAH55020.1.
IPI	IPI00554933. IPI00881469.
PIR	S71878.
RefSeq	NP_032211.3. NM_008185.3.
UniGene	Mm.2746.
3D structure databases
ProteinModelPortal	Q64471.
SMR	Q64471. Positions 3-240.
ModBase	Search...
PTM databases
PhosphoSite	Q64471.
2D gel databases
SWISS-2DPAGE	Q64471.
Proteomic databases
PaxDb	Q64471.
PRIDE	Q64471.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000001713; ENSMUSP00000001713; ENSMUSG00000001663.
GeneID	14871.
KEGG	mmu:14871.
Organism-specific databases
CTD	2952.
MGI	MGI:107379. Gstt1.
Phylogenomic databases
eggNOG	COG0625.
GeneTree	ENSGT00540000069741.
HOGENOM	HOG000125747.
HOVERGEN	HBG051854.
InParanoid	Q64471.
KO	K00799.
OMA	RRNCLRT.
OrthoDB	EOG43N7F8.
Enzyme and pathway databases
SABIO-RK	Q64471.
Gene expression databases
CleanEx	MM_GSTT1.
Genevestigator	Q64471.
GermOnline	ENSMUSG00000001663. Mus musculus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	287137.
SOURCE	Search...

Entry information

Entry name

GSTT1_MOUSE

Accession

Primary (citable) accession number: Q64471
Secondary accession number(s): Q91X50

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 96 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program