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Q64471

- GSTT1_MOUSE

UniProt

Q64471 - GSTT1_MOUSE

Protein

Glutathione S-transferase theta-1

Gene

Gstt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 4 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Also binds steroids, bilirubin, carcinogens and numerous organic anions. Has dichloromethane dehalogenase activity.1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione peroxidase activity Source: Ensembl
    2. glutathione transferase activity Source: UniProtKB

    GO - Biological processi

    1. glutathione metabolic process Source: UniProtKB
    2. response to drug Source: Ensembl
    3. response to organic cyclic compound Source: Ensembl

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    ReactomeiREACT_215316. Glutathione conjugation.
    SABIO-RKQ64471.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase theta-1 (EC:2.5.1.18)
    Alternative name(s):
    GST class-theta-1
    Gene namesi
    Name:Gstt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:107379. Gstt1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi234 – 2341R → W: Strongly reduced catalytic activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 240239Glutathione S-transferase theta-1PRO_0000185940Add
    BLAST

    Proteomic databases

    PaxDbiQ64471.
    PRIDEiQ64471.

    2D gel databases

    SWISS-2DPAGEQ64471.

    PTM databases

    PhosphoSiteiQ64471.

    Expressioni

    Tissue specificityi

    In liver, highest expression found in central vein limiting plate hepatocytes. Also expressed in interlobular bile duct epithelial cells. In lung, expressed in Clara cells and ciliated cells of the bronchiolar epithelium and in type II alveolar cells of the lung parenchyma.

    Gene expression databases

    ArrayExpressiQ64471.
    CleanExiMM_GSTT1.
    GenevestigatoriQ64471.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiQ64471. 2 interactions.
    MINTiMINT-1865860.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64471.
    SMRiQ64471. Positions 3-240.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 8281GST N-terminalAdd
    BLAST
    Domaini88 – 222135GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 542Glutathione bindingBy similarity
    Regioni66 – 672Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Theta family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    GeneTreeiENSGT00540000069741.
    HOGENOMiHOG000125747.
    HOVERGENiHBG051854.
    InParanoidiQ64471.
    KOiK00799.
    OMAiRRNCLRT.
    OrthoDBiEOG7GTT59.
    TreeFamiTF325759.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q64471-1 [UniParc]FASTAAdd to Basket

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    MVLELYLDLL SQPCRAIYIF AKKNNIPFQM HTVELRKGEH LSDAFARVNP    50
    MKRVPAMMDG GFTLCESVAI LLYLAHKYKV PDHWYPQDLQ ARARVDEYLA 100
    WQHTGLRRSC LRALWHKVMF PVFLGEQIPP ETLAATLAEL DVNLQVLEDK 150
    FLQDKDFLVG PHISLADLVA ITELMHPVGG GCPVFEGHPR LAAWYQRVEA 200
    AVGKDLFREA HEVILKVKDC PPADLIIKQK LMPRVLAMIQ 240
    Length:240
    Mass (Da):27,374
    Last modified:October 3, 2012 - v4
    Checksum:iEDCB477D557466DB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531R → K in CAA66665. (PubMed:8761485)Curated
    Sequence conflicti237 – 2371A → T in CAA66665. (PubMed:8761485)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98055 mRNA. Translation: CAA66665.1.
    AC142499 Genomic DNA. No translation available.
    CH466553 Genomic DNA. Translation: EDL31888.1.
    BC012254 mRNA. Translation: AAH12254.1.
    BC055020 mRNA. Translation: AAH55020.1.
    CCDSiCCDS23932.1.
    PIRiS71878.
    RefSeqiNP_032211.3. NM_008185.3.
    UniGeneiMm.2746.

    Genome annotation databases

    EnsembliENSMUST00000001713; ENSMUSP00000001713; ENSMUSG00000001663.
    GeneIDi14871.
    KEGGimmu:14871.
    UCSCiuc007frc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98055 mRNA. Translation: CAA66665.1 .
    AC142499 Genomic DNA. No translation available.
    CH466553 Genomic DNA. Translation: EDL31888.1 .
    BC012254 mRNA. Translation: AAH12254.1 .
    BC055020 mRNA. Translation: AAH55020.1 .
    CCDSi CCDS23932.1.
    PIRi S71878.
    RefSeqi NP_032211.3. NM_008185.3.
    UniGenei Mm.2746.

    3D structure databases

    ProteinModelPortali Q64471.
    SMRi Q64471. Positions 3-240.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q64471. 2 interactions.
    MINTi MINT-1865860.

    PTM databases

    PhosphoSitei Q64471.

    2D gel databases

    SWISS-2DPAGE Q64471.

    Proteomic databases

    PaxDbi Q64471.
    PRIDEi Q64471.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000001713 ; ENSMUSP00000001713 ; ENSMUSG00000001663 .
    GeneIDi 14871.
    KEGGi mmu:14871.
    UCSCi uc007frc.2. mouse.

    Organism-specific databases

    CTDi 2952.
    MGIi MGI:107379. Gstt1.

    Phylogenomic databases

    eggNOGi COG0625.
    GeneTreei ENSGT00540000069741.
    HOGENOMi HOG000125747.
    HOVERGENi HBG051854.
    InParanoidi Q64471.
    KOi K00799.
    OMAi RRNCLRT.
    OrthoDBi EOG7GTT59.
    TreeFami TF325759.

    Enzyme and pathway databases

    Reactomei REACT_215316. Glutathione conjugation.
    SABIO-RK Q64471.

    Miscellaneous databases

    NextBioi 287137.
    PROi Q64471.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64471.
    CleanExi MM_GSTT1.
    Genevestigatori Q64471.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human."
      Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T.
      Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X C3H.
      Tissue: Liver and Lung.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver and Salivary gland.
    5. "Residue 234 is a master switch of the alternative-substrate activity profile of human and rodent theta class glutathione transferase T1-1."
      Shokeer A., Mannervik B.
      Biochim. Biophys. Acta 1800:466-473(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ARG-234.

    Entry informationi

    Entry nameiGSTT1_MOUSE
    AccessioniPrimary (citable) accession number: Q64471
    Secondary accession number(s): Q91X50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 107 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3