Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase theta-1

Gene

Gstt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Also binds steroids, bilirubin, carcinogens and numerous organic anions. Has dichloromethane dehalogenase activity.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401GlutathioneBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

SABIO-RKQ64471.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase theta-1 (EC:2.5.1.18)
Alternative name(s):
GST class-theta-1
Gene namesi
Name:Gstt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:107379. Gstt1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi234 – 2341R → W: Strongly reduced catalytic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 240239Glutathione S-transferase theta-1PRO_0000185940Add
BLAST

Proteomic databases

PaxDbiQ64471.
PRIDEiQ64471.

2D gel databases

SWISS-2DPAGEQ64471.

PTM databases

PhosphoSiteiQ64471.

Expressioni

Tissue specificityi

In liver, highest expression found in central vein limiting plate hepatocytes. Also expressed in interlobular bile duct epithelial cells. In lung, expressed in Clara cells and ciliated cells of the bronchiolar epithelium and in type II alveolar cells of the lung parenchyma.

Gene expression databases

CleanExiMM_GSTT1.
ExpressionAtlasiQ64471. baseline and differential.
GenevestigatoriQ64471.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiQ64471. 2 interactions.
MINTiMINT-1865860.

Structurei

3D structure databases

ProteinModelPortaliQ64471.
SMRiQ64471. Positions 3-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8281GST N-terminalAdd
BLAST
Domaini88 – 222135GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 542Glutathione bindingBy similarity
Regioni66 – 672Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00540000069741.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiQ64471.
KOiK00799.
OMAiKQKLMPA.
OrthoDBiEOG7GTT59.
TreeFamiTF325759.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLELYLDLL SQPCRAIYIF AKKNNIPFQM HTVELRKGEH LSDAFARVNP
60 70 80 90 100
MKRVPAMMDG GFTLCESVAI LLYLAHKYKV PDHWYPQDLQ ARARVDEYLA
110 120 130 140 150
WQHTGLRRSC LRALWHKVMF PVFLGEQIPP ETLAATLAEL DVNLQVLEDK
160 170 180 190 200
FLQDKDFLVG PHISLADLVA ITELMHPVGG GCPVFEGHPR LAAWYQRVEA
210 220 230 240
AVGKDLFREA HEVILKVKDC PPADLIIKQK LMPRVLAMIQ
Length:240
Mass (Da):27,374
Last modified:October 3, 2012 - v4
Checksum:iEDCB477D557466DB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531R → K in CAA66665 (PubMed:8761485).Curated
Sequence conflicti237 – 2371A → T in CAA66665 (PubMed:8761485).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98055 mRNA. Translation: CAA66665.1.
AC142499 Genomic DNA. No translation available.
CH466553 Genomic DNA. Translation: EDL31888.1.
BC012254 mRNA. Translation: AAH12254.1.
BC055020 mRNA. Translation: AAH55020.1.
CCDSiCCDS23932.1.
PIRiS71878.
RefSeqiNP_032211.3. NM_008185.3.
UniGeneiMm.2746.

Genome annotation databases

EnsembliENSMUST00000001713; ENSMUSP00000001713; ENSMUSG00000001663.
GeneIDi14871.
KEGGimmu:14871.
UCSCiuc007frc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98055 mRNA. Translation: CAA66665.1.
AC142499 Genomic DNA. No translation available.
CH466553 Genomic DNA. Translation: EDL31888.1.
BC012254 mRNA. Translation: AAH12254.1.
BC055020 mRNA. Translation: AAH55020.1.
CCDSiCCDS23932.1.
PIRiS71878.
RefSeqiNP_032211.3. NM_008185.3.
UniGeneiMm.2746.

3D structure databases

ProteinModelPortaliQ64471.
SMRiQ64471. Positions 3-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64471. 2 interactions.
MINTiMINT-1865860.

PTM databases

PhosphoSiteiQ64471.

2D gel databases

SWISS-2DPAGEQ64471.

Proteomic databases

PaxDbiQ64471.
PRIDEiQ64471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001713; ENSMUSP00000001713; ENSMUSG00000001663.
GeneIDi14871.
KEGGimmu:14871.
UCSCiuc007frc.2. mouse.

Organism-specific databases

CTDi2952.
MGIiMGI:107379. Gstt1.

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00540000069741.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiQ64471.
KOiK00799.
OMAiKQKLMPA.
OrthoDBiEOG7GTT59.
TreeFamiTF325759.

Enzyme and pathway databases

SABIO-RKQ64471.

Miscellaneous databases

NextBioi287137.
PROiQ64471.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GSTT1.
ExpressionAtlasiQ64471. baseline and differential.
GenevestigatoriQ64471.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human."
    Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T.
    Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X C3H.
    Tissue: Liver and Lung.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver and Salivary gland.
  5. "Residue 234 is a master switch of the alternative-substrate activity profile of human and rodent theta class glutathione transferase T1-1."
    Shokeer A., Mannervik B.
    Biochim. Biophys. Acta 1800:466-473(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ARG-234.

Entry informationi

Entry nameiGSTT1_MOUSE
AccessioniPrimary (citable) accession number: Q64471
Secondary accession number(s): Q91X50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 3, 2012
Last modified: April 1, 2015
This is version 111 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.