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Protein

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific

Gene

Gapdhs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm7293), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm3839)
  2. Phosphoglycerate kinase 2 (Pgk2), Phosphoglycerate kinase 1 (Pgk1)
  3. no protein annotated in this organism
  4. Gamma-enolase (Eno2), Beta-enolase (Eno3), Alpha-enolase (Eno1), Enolase 4 (Eno4)
  5. Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKLR (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKM (Pkm)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei138 – 1381NADBy similarity
Binding sitei183 – 1831NAD; via carbonyl oxygenBy similarity
Binding sitei205 – 2051NADBy similarity
Binding sitei225 – 2251NADBy similarity
Active sitei256 – 2561NucleophilePROSITE-ProRule annotation
Sitei283 – 2831Activates thiol group during catalysisBy similarity
Binding sitei286 – 2861Glyceraldehyde 3-phosphateBy similarity
Binding sitei338 – 3381Glyceraldehyde 3-phosphateBy similarity
Binding sitei420 – 4201NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi117 – 1182NADBy similarity

GO - Molecular functioni

GO - Biological processi

  • glucose metabolic process Source: InterPro
  • glycolytic process Source: MGI
  • positive regulation of glycolytic process Source: UniProtKB
  • sperm motility Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (EC:1.2.1.12)
Alternative name(s):
Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
Short name:
GAPDH-2
Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:Gapdhs
Synonyms:Gapd-s, Gapds
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:95653. Gapdhs.

Subcellular locationi

GO - Cellular componenti

  • cilium Source: MGI
  • cytoplasm Source: UniProtKB-SubCell
  • motile cilium Source: MGI
  • nucleus Source: MGI
  • sperm fibrous sheath Source: MGI
  • sperm principal piece Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice display greatly reduced ATP levels in sperm, severely impaired sperm motility and are infertile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Glyceraldehyde-3-phosphate dehydrogenase, testis-specificPRO_0000145504Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei358 – 3581PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ64467.
MaxQBiQ64467.
PaxDbiQ64467.
PRIDEiQ64467.

PTM databases

iPTMnetiQ64467.
PhosphoSiteiQ64467.
SwissPalmiQ64467.

Expressioni

Tissue specificityi

Testis specific.

Developmental stagei

First expressed at day 20 in post-meiotic germ cells. Levels increase until day 24 and then remain constant during maturity.

Gene expression databases

BgeeiQ64467.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ64467. 2 interactions.
MINTiMINT-4095460.
STRINGi10090.ENSMUSP00000074317.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5C7IX-ray2.01O/R107-439[»]
ProteinModelPortaliQ64467.
SMRiQ64467. Positions 108-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 105105Testis-specific N-terminal extensionBy similarityAdd
BLAST
Regioni255 – 2573Glyceraldehyde 3-phosphate bindingBy similarity
Regioni315 – 3162Glyceraldehyde 3-phosphate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi21 – 4121Cys/Pro-richAdd
BLAST
Compositional biasi54 – 7320Poly-ProAdd
BLAST
Compositional biasi84 – 10017Poly-ProAdd
BLAST

Domaini

The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-33 with sheath components.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071678.
HOVERGENiHBG000227.
InParanoidiQ64467.
KOiK10705.
OrthoDBiEOG7Q5HDF.
PhylomeDBiQ64467.
TreeFamiTF300533.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRDVVLTN VTVVQLRRDR CPCPCPCPCP CPCPVIRPPP PKLEDPPPTV
60 70 80 90 100
EEQPPPPPPP PPPPPPPPPP PPPQIEPDKF EEAPPPPPPP PPPPPPPPPP
110 120 130 140 150
LQKPARELTV GINGFGRIGR LVLRVCMEKG IRVVAVNDPF IDPEYMVYMF
160 170 180 190 200
KYDSTHGRYK GNVEHKNGQL VVDNLEINTY QCKDPKEIPW SSIGNPYVVE
210 220 230 240 250
CTGVYLSIEA ASAHISSGAR RVVVTAPSPD APMFVMGVNE KDYNPGSMTI
260 270 280 290 300
VSNASCTTNC LAPLAKVIHE NFGIVEGLMT TVHSYTATQK TVDGPSKKDW
310 320 330 340 350
RGGRGAHQNI IPSSTGAAKA VGKVIPELKG KLTGMAFRVP TPNVSVVDLT
360 370 380 390 400
CRLAKPASYS AITEAVKAAA KGPLAGILAY TEDQVVSTDF NGNPHSSIFD
410 420 430 440
AKAGIALNDN FVKLVAWYDN EYGYSNRVVD LLRYMFSREK
Length:440
Mass (Da):47,657
Last modified:November 1, 1996 - v1
Checksum:i05FF0A093D1ABD9C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 342Missing in AAA80276 (PubMed:7736666).Curated
Sequence conflicti43 – 431L → V in AAA80276 (PubMed:7736666).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60978 mRNA. Translation: AAA53033.1.
U09964 Genomic DNA. Translation: AAA80276.1.
CCDSiCCDS71933.1.
PIRiI49681.
RefSeqiNP_032111.1. NM_008085.2.
UniGeneiMm.436562.

Genome annotation databases

GeneIDi14447.
KEGGimmu:14447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60978 mRNA. Translation: AAA53033.1.
U09964 Genomic DNA. Translation: AAA80276.1.
CCDSiCCDS71933.1.
PIRiI49681.
RefSeqiNP_032111.1. NM_008085.2.
UniGeneiMm.436562.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5C7IX-ray2.01O/R107-439[»]
ProteinModelPortaliQ64467.
SMRiQ64467. Positions 108-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64467. 2 interactions.
MINTiMINT-4095460.
STRINGi10090.ENSMUSP00000074317.

PTM databases

iPTMnetiQ64467.
PhosphoSiteiQ64467.
SwissPalmiQ64467.

Proteomic databases

EPDiQ64467.
MaxQBiQ64467.
PaxDbiQ64467.
PRIDEiQ64467.

Protocols and materials databases

DNASUi14447.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi14447.
KEGGimmu:14447.

Organism-specific databases

CTDi26330.
MGIiMGI:95653. Gapdhs.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071678.
HOVERGENiHBG000227.
InParanoidiQ64467.
KOiK10705.
OrthoDBiEOG7Q5HDF.
PhylomeDBiQ64467.
TreeFamiTF300533.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Miscellaneous databases

ChiTaRSiGapdhs. mouse.
NextBioi286061.
PROiQ64467.
SOURCEiSearch...

Gene expression databases

BgeeiQ64467.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of a glyceraldehyde 3-phosphate dehydrogenase gene specific to mouse spermatogenic cells."
    Welch J.E., Schatte E.C., O'Brien D.A., Eddy E.M.
    Biol. Reprod. 46:869-878(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Testis.
  2. "Genomic organization of a mouse glyceraldehyde 3-phosphate dehydrogenase gene (Gapd-s) expressed in post-meiotic spermatogenic cells."
    Welch J.E., Brown P.R., O'Brien D.A., Eddy E.M.
    Dev. Genet. 16:179-189(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ICR X Swiss Webster.
    Tissue: Testis.
  3. "Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility."
    Miki K., Qu W., Goulding E.H., Willis W.D., Bunch D.O., Strader L.F., Perreault S.D., Eddy E.M., O'Brien D.A.
    Proc. Natl. Acad. Sci. U.S.A. 101:16501-16506(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiG3PT_MOUSE
AccessioniPrimary (citable) accession number: Q64467
Secondary accession number(s): Q60650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.