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Q64462 (CP4B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 4B1

EC=1.14.14.1
Alternative name(s):
CYPIVB1
Gene names
Name:Cyp4b1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for mutagenic activation of 3-methoxy-4-aminoazobenzene (3-MeO-AAB); a potent procarcinogen. Also active on 2-aminofluorene and 2-aminoanthracene.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Present abundantly in renal microsomes of male mice but not in those of female mice. Also present in pulmonary microsomes of male and female mice. Not found in liver.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 511510Cytochrome P450 4B1
PRO_0000051820

Sites

Metal binding4531Iron (heme axial ligand) By similarity
Binding site3151Heme (covalent; via 1 link) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64462 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 35463ACDFFE6328B

FASTA51158,900
        10         20         30         40         50         60 
MALSFLSPSL SRLGLWASVV ILMVTVLKLL SLLFRRQKLA RALDSFPGPP KHWLFGHALE 

        70         80         90        100        110        120 
IQKTGGLDKV VTWTEQFPYA HPLWLGQFIV FLNIYEPDYA KAVYSRGDPK AAYVYDFFLQ 

       130        140        150        160        170        180 
WIGKGLLVLE GPKWFQHRKL LTPGFHYDVL KPYVAIFAES TRVMLDKWEK KASENKSFDI 

       190        200        210        220        230        240 
FCDVGHMALD TLMKCTFGKG DSGLSHSDNS YYLAVSDLTL LMQQRIDSFQ YHNDFIYWLT 

       250        260        270        280        290        300 
PHGRRFLRAC QIAHDHTDHV IRQRKAALQD EKEQKKLQER RHLDFLDILL GARDESGIKL 

       310        320        330        340        350        360 
SDADLRAEVD TFMFEGHDTT TSGISWFLYC MALYPMHQQR CREEVREILG DRDSFQWDDL 

       370        380        390        400        410        420 
AQMTYLTMCM KECFRLYPPV PQVYRQLSKP VTFVDGRSLP AGSLISLHIY ALHRNSAVWP 

       430        440        450        460        470        480 
DPEVFDPLRF SPENMTGRHP FAFMPFSAGP RNCIGQQFAM NEMKVVTALC LLRFEFSPDP 

       490        500        510 
SKIPIKVPQL ILRSKNGIHL YLKPLGPGSG K 

« Hide

References

« Hide 'large scale' references
[1]"Mutagenic activation of 3-methoxy-4-aminoazobenzene by mouse renal cytochrome P450 CYP4B1: cloning and characterization of mouse CYP4B1."
Imaoka S., Hiroi T., Tamura Y., Yamazaki H., Shimada T., Komori M., Degawa M., Funae Y.
Arch. Biochem. Biophys. 321:255-262(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: BALB/c.
Tissue: Kidney.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50834 mRNA. Translation: BAA09446.1.
BC008996 mRNA. Translation: AAH08996.1.
CCDSCCDS18493.1.
PIRS66472.
RefSeqNP_031849.1. NM_007823.2.
UniGeneMm.1840.

3D structure databases

ProteinModelPortalQ64462.
SMRQ64462. Positions 65-506.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ64462. 3 interactions.
MINTMINT-4091779.
STRING10090.ENSMUSP00000099768.

PTM databases

PhosphoSiteQ64462.

Proteomic databases

MaxQBQ64462.
PaxDbQ64462.
PRIDEQ64462.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102707; ENSMUSP00000099768; ENSMUSG00000028713.
GeneID13120.
KEGGmmu:13120.
UCSCuc008ufc.1. mouse.

Organism-specific databases

CTD1580.
MGIMGI:103225. Cyp4b1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00740000115505.
HOGENOMHOG000233833.
HOVERGENHBG000182.
InParanoidQ64462.
KOK07426.
OMAKWLIATA.
OrthoDBEOG7CNZFK.
PhylomeDBQ64462.
TreeFamTF105088.

Gene expression databases

ArrayExpressQ64462.
BgeeQ64462.
GenevestigatorQ64462.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283154.
PROQ64462.
SOURCESearch...

Entry information

Entry nameCP4B1_MOUSE
AccessionPrimary (citable) accession number: Q64462
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot