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Protein

Cytochrome P450 4B1

Gene

Cyp4b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for mutagenic activation of 3-methoxy-4-aminoazobenzene (3-MeO-AAB); a potent procarcinogen. Also active on 2-aminofluorene and 2-aminoanthracene.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei315 – 3151Heme (covalent; via 1 link)By similarity
Metal bindingi453 – 4531Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. drug binding Source: Ensembl
  3. fluorene oxygenase activity Source: Ensembl
  4. heme binding Source: InterPro
  5. iron ion binding Source: InterPro

GO - Biological processi

  1. biphenyl metabolic process Source: Ensembl
  2. exogenous drug catabolic process Source: Ensembl
  3. fluorene metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_331519. Fatty acids.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4B1 (EC:1.14.14.1)
Alternative name(s):
CYPIVB1
Gene namesi
Name:Cyp4b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:103225. Cyp4b1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 511510Cytochrome P450 4B1PRO_0000051820Add
BLAST

Proteomic databases

MaxQBiQ64462.
PaxDbiQ64462.
PRIDEiQ64462.

PTM databases

PhosphoSiteiQ64462.

Expressioni

Tissue specificityi

Present abundantly in renal microsomes of male mice but not in those of female mice. Also present in pulmonary microsomes of male and female mice. Not found in liver.

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

BgeeiQ64462.
ExpressionAtlasiQ64462. baseline and differential.
GenevestigatoriQ64462.

Interactioni

Protein-protein interaction databases

IntActiQ64462. 3 interactions.
MINTiMINT-4091779.
STRINGi10090.ENSMUSP00000099768.

Structurei

3D structure databases

ProteinModelPortaliQ64462.
SMRiQ64462. Positions 47-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiQ64462.
KOiK07426.
OMAiKWLIATA.
OrthoDBiEOG7CNZFK.
PhylomeDBiQ64462.
TreeFamiTF105088.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSFLSPSL SRLGLWASVV ILMVTVLKLL SLLFRRQKLA RALDSFPGPP
60 70 80 90 100
KHWLFGHALE IQKTGGLDKV VTWTEQFPYA HPLWLGQFIV FLNIYEPDYA
110 120 130 140 150
KAVYSRGDPK AAYVYDFFLQ WIGKGLLVLE GPKWFQHRKL LTPGFHYDVL
160 170 180 190 200
KPYVAIFAES TRVMLDKWEK KASENKSFDI FCDVGHMALD TLMKCTFGKG
210 220 230 240 250
DSGLSHSDNS YYLAVSDLTL LMQQRIDSFQ YHNDFIYWLT PHGRRFLRAC
260 270 280 290 300
QIAHDHTDHV IRQRKAALQD EKEQKKLQER RHLDFLDILL GARDESGIKL
310 320 330 340 350
SDADLRAEVD TFMFEGHDTT TSGISWFLYC MALYPMHQQR CREEVREILG
360 370 380 390 400
DRDSFQWDDL AQMTYLTMCM KECFRLYPPV PQVYRQLSKP VTFVDGRSLP
410 420 430 440 450
AGSLISLHIY ALHRNSAVWP DPEVFDPLRF SPENMTGRHP FAFMPFSAGP
460 470 480 490 500
RNCIGQQFAM NEMKVVTALC LLRFEFSPDP SKIPIKVPQL ILRSKNGIHL
510
YLKPLGPGSG K
Length:511
Mass (Da):58,900
Last modified:November 1, 1996 - v1
Checksum:i35463ACDFFE6328B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50834 mRNA. Translation: BAA09446.1.
BC008996 mRNA. Translation: AAH08996.1.
CCDSiCCDS18493.1.
PIRiS66472.
RefSeqiNP_031849.1. NM_007823.2.
UniGeneiMm.1840.

Genome annotation databases

EnsembliENSMUST00000102707; ENSMUSP00000099768; ENSMUSG00000028713.
GeneIDi13120.
KEGGimmu:13120.
UCSCiuc008ufc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50834 mRNA. Translation: BAA09446.1.
BC008996 mRNA. Translation: AAH08996.1.
CCDSiCCDS18493.1.
PIRiS66472.
RefSeqiNP_031849.1. NM_007823.2.
UniGeneiMm.1840.

3D structure databases

ProteinModelPortaliQ64462.
SMRiQ64462. Positions 47-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64462. 3 interactions.
MINTiMINT-4091779.
STRINGi10090.ENSMUSP00000099768.

PTM databases

PhosphoSiteiQ64462.

Proteomic databases

MaxQBiQ64462.
PaxDbiQ64462.
PRIDEiQ64462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102707; ENSMUSP00000099768; ENSMUSG00000028713.
GeneIDi13120.
KEGGimmu:13120.
UCSCiuc008ufc.1. mouse.

Organism-specific databases

CTDi1580.
MGIiMGI:103225. Cyp4b1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiQ64462.
KOiK07426.
OMAiKWLIATA.
OrthoDBiEOG7CNZFK.
PhylomeDBiQ64462.
TreeFamiTF105088.

Enzyme and pathway databases

ReactomeiREACT_331519. Fatty acids.

Miscellaneous databases

NextBioi283154.
PROiQ64462.
SOURCEiSearch...

Gene expression databases

BgeeiQ64462.
ExpressionAtlasiQ64462. baseline and differential.
GenevestigatoriQ64462.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutagenic activation of 3-methoxy-4-aminoazobenzene by mouse renal cytochrome P450 CYP4B1: cloning and characterization of mouse CYP4B1."
    Imaoka S., Hiroi T., Tamura Y., Yamazaki H., Shimada T., Komori M., Degawa M., Funae Y.
    Arch. Biochem. Biophys. 321:255-262(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: BALB/c.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiCP4B1_MOUSE
AccessioniPrimary (citable) accession number: Q64462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.