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Protein

Gap junction alpha-3 protein

Gene

Gja3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

GO - Molecular functioni

  1. gap junction channel activity Source: InterPro

GO - Biological processi

  1. cell communication Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction alpha-3 protein
Alternative name(s):
Connexin-46
Short name:
Cx46
Gene namesi
Name:Gja3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:95714. Gja3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei20 – 4021HelicalSequence AnalysisAdd
BLAST
Topological domaini41 – 7636ExtracellularSequence AnalysisAdd
BLAST
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST
Topological domaini98 – 15861CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei159 – 17921HelicalSequence AnalysisAdd
BLAST
Topological domaini180 – 20728ExtracellularSequence AnalysisAdd
BLAST
Transmembranei208 – 22821HelicalSequence AnalysisAdd
BLAST
Topological domaini229 – 417189CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. connexon complex Source: InterPro
  2. gap junction Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Gap junction alpha-3 proteinPRO_0000057811Add
BLAST

Proteomic databases

PRIDEiQ64448.

PTM databases

PhosphoSiteiQ64448.

Expressioni

Gene expression databases

BgeeiQ64448.
CleanExiMM_GJA3.
ExpressionAtlasiQ64448. baseline and differential.
GenevestigatoriQ64448.

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins. This particular connexin is a component of lens fiber gap junctions, can form both junctional and non-junctional ("hemi-") channels.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059587.

Structurei

3D structure databases

ProteinModelPortaliQ64448.
SMRiQ64448. Positions 3-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47291.
GeneTreeiENSGT00760000118780.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiQ64448.
KOiK07612.
OMAiIGFPPYY.
OrthoDBiEOG7P2XSS.
TreeFamiTF329606.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002262. Connexin46.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PfamiPF00029. Connexin. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01133. CONNEXINA3.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDWSFLGRL LENAQEHSTV IGKVWLTVLF IFRILVLGAA AEEVWGDEQS
60 70 80 90 100
DFTCNTQQPG CENVCYDRAF PISHIRFWAL QIIFVSTPTL IYLGHVLHIV
110 120 130 140 150
RMEEKKKERE EELLRRDNPQ HGRGREPMRT GSPRDPPLRD DRGKVRIAGA
160 170 180 190 200
LLRTYVFNII FKTLFEVGFI AGQYFLYGFQ LQPLYRCDRW PCPNTVDCFI
210 220 230 240 250
SRPTEKTIFV IFMLAVACAS LVLNMLEIYH LGWKKLKQGV TNHFNPDASE
260 270 280 290 300
ARHKPLDPLP TATSSGPPSV SIGFPPYYTH PACPTVQAKA IGFPGAPLSP
310 320 330 340 350
ADFTVVTLND AQGRNHPVKH CNGHHLTTEQ NWTRQVAEQQ TPASKPSSAA
360 370 380 390 400
SSPDGRKGLI DSSGSSLQES ALVVTPEEGE QALATTVEMH SPPLVLLDPG
410
RSSKSSNGRA RPGDLAI
Length:417
Mass (Da):46,321
Last modified:July 27, 2011 - v4
Checksum:iFF235977E26F55F0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611T → A in AAB03211 (Ref. 1) Curated
Sequence conflicti288 – 2881A → G in AAB03211 (Ref. 1) Curated
Sequence conflicti390 – 3901H → Y in AAB03211 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44955 Genomic DNA. Translation: AAB03211.1.
CH466535 Genomic DNA. Translation: EDL36182.1.
CH466535 Genomic DNA. Translation: EDL36184.1.
BC138492 mRNA. Translation: AAI38493.1.
BC138493 mRNA. Translation: AAI38494.1.
CCDSiCCDS27152.1.
RefSeqiNP_001258552.1. NM_001271623.1.
NP_058671.2. NM_016975.3.
XP_006518622.1. XM_006518559.2.
UniGeneiMm.57207.

Genome annotation databases

EnsembliENSMUST00000061614; ENSMUSP00000059587; ENSMUSG00000048582.
GeneIDi14611.
KEGGimmu:14611.
UCSCiuc007ucv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44955 Genomic DNA. Translation: AAB03211.1.
CH466535 Genomic DNA. Translation: EDL36182.1.
CH466535 Genomic DNA. Translation: EDL36184.1.
BC138492 mRNA. Translation: AAI38493.1.
BC138493 mRNA. Translation: AAI38494.1.
CCDSiCCDS27152.1.
RefSeqiNP_001258552.1. NM_001271623.1.
NP_058671.2. NM_016975.3.
XP_006518622.1. XM_006518559.2.
UniGeneiMm.57207.

3D structure databases

ProteinModelPortaliQ64448.
SMRiQ64448. Positions 3-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059587.

PTM databases

PhosphoSiteiQ64448.

Proteomic databases

PRIDEiQ64448.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061614; ENSMUSP00000059587; ENSMUSG00000048582.
GeneIDi14611.
KEGGimmu:14611.
UCSCiuc007ucv.1. mouse.

Organism-specific databases

CTDi2700.
MGIiMGI:95714. Gja3.

Phylogenomic databases

eggNOGiNOG47291.
GeneTreeiENSGT00760000118780.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiQ64448.
KOiK07612.
OMAiIGFPPYY.
OrthoDBiEOG7P2XSS.
TreeFamiTF329606.

Miscellaneous databases

NextBioi286412.
PROiQ64448.
SOURCEiSearch...

Gene expression databases

BgeeiQ64448.
CleanExiMM_GJA3.
ExpressionAtlasiQ64448. baseline and differential.
GenevestigatoriQ64448.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002262. Connexin46.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PfamiPF00029. Connexin. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01133. CONNEXINA3.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and expression of a lens gap junction connexin."
    Kumar N.M., Nishi M., Klier F.G., Gilula N.B.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6 X CBA.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiCXA3_MOUSE
AccessioniPrimary (citable) accession number: Q64448
Secondary accession number(s): B2RRM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 110 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.