Q64448 (CXA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gap junction alpha-3 protein

Alternative name(s):
Connexin-46
Short name=Cx46

Gene names

Name:

Gja3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins. This particular connexin is a component of lens fiber gap junctions, can form both junctional and non-junctional ("hemi-") channels.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Sequence similarities	Belongs to the connexin family. Alpha-type (group II) subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell communication Inferred from electronic annotation. Source: InterPro
Cellular_component	connexon complex Inferred from electronic annotation. Source: InterPro gap junction Inferred from direct assay PubMed 11786642. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 9620080. Source: MGI
Molecular_function	gap junction channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Gap junction alpha-3 protein		PRO_0000057811
Regions
	Topological domain	1 – 19	19	Cytoplasmic Potential
	Transmembrane	20 – 40	21	Helical; Potential
	Topological domain	41 – 76	36	Extracellular Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Topological domain	98 – 158	61	Cytoplasmic Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Topological domain	180 – 207	28	Extracellular Potential
	Transmembrane	208 – 228	21	Helical; Potential
	Topological domain	229 – 417	189	Cytoplasmic Potential
Experimental info
	Sequence conflict	261	1	T → A in AAB03211. Ref.1
	Sequence conflict	288	1	A → G in AAB03211. Ref.1
	Sequence conflict	390	1	H → Y in AAB03211. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q64448 [UniParc]. Last modified July 27, 2011. Version 4. Checksum: FF235977E26F55F0	FASTA	417	46,321
	10 20 30 40 50 60 MGDWSFLGRL LENAQEHSTV IGKVWLTVLF IFRILVLGAA AEEVWGDEQS DFTCNTQQPG 70 80 90 100 110 120 CENVCYDRAF PISHIRFWAL QIIFVSTPTL IYLGHVLHIV RMEEKKKERE EELLRRDNPQ 130 140 150 160 170 180 HGRGREPMRT GSPRDPPLRD DRGKVRIAGA LLRTYVFNII FKTLFEVGFI AGQYFLYGFQ 190 200 210 220 230 240 LQPLYRCDRW PCPNTVDCFI SRPTEKTIFV IFMLAVACAS LVLNMLEIYH LGWKKLKQGV 250 260 270 280 290 300 TNHFNPDASE ARHKPLDPLP TATSSGPPSV SIGFPPYYTH PACPTVQAKA IGFPGAPLSP 310 320 330 340 350 360 ADFTVVTLND AQGRNHPVKH CNGHHLTTEQ NWTRQVAEQQ TPASKPSSAA SSPDGRKGLI 370 380 390 400 410 DSSGSSLQES ALVVTPEEGE QALATTVEMH SPPLVLLDPG RSSKSSNGRA RPGDLAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and expression of a lens gap junction connexin." Kumar N.M., Nishi M., Klier F.G., Gilula N.B. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X CBA.
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U44955 Genomic DNA. Translation: AAB03211.1. CH466535 Genomic DNA. Translation: EDL36182.1. CH466535 Genomic DNA. Translation: EDL36184.1. BC138492 mRNA. Translation: AAI38493.1. BC138493 mRNA. Translation: AAI38494.1.
IPI	IPI00226997.
RefSeq	NP_001258552.1. NM_001271623.1. NP_058671.2. NM_016975.3.
UniGene	Mm.393732. Mm.57207.
3D structure databases
ProteinModelPortal	Q64448.
SMR	Q64448. Positions 3-231.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000059587.
PTM databases
PhosphoSite	Q64448.
Proteomic databases
PRIDE	Q64448.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000061614; ENSMUSP00000059587; ENSMUSG00000048582.
GeneID	14611.
KEGG	mmu:14611.
Organism-specific databases
CTD	2700.
MGI	MGI:95714. Gja3.
Phylogenomic databases
eggNOG	NOG47291.
GeneTree	ENSGT00700000104145.
HOGENOM	HOG000231127.
HOVERGEN	HBG009576.
InParanoid	B2RRM1.
KO	K07612.
OMA	KVRIAGA.
OrthoDB	EOG4868D1.
Gene expression databases
ArrayExpress	Q64448.
Bgee	Q64448.
CleanEx	MM_GJA3.
Genevestigator	Q64448.
GermOnline	ENSMUSG00000048582. Mus musculus.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002262. Connexin46. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. [Graphical view]
PANTHER	PTHR11984. PTHR11984. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01133. CONNEXINA3.
SMART	SM00037. CNX. 1 hit. SM01089. Connexin_CCC. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286412.
SOURCE	Search...

Entry information

Entry name

CXA3_MOUSE

Accession

Primary (citable) accession number: Q64448
Secondary accession number(s): B2RRM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 94 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program