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Q64444 (CAH4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbonic anhydrase 4

EC=4.2.1.1
Alternative name(s):
Carbonate dehydratase IV
Carbonic anhydrase IV
Short name=CA-IV
Gene names
Name:Ca4
Synonyms:Car4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 By similarity.

Catalytic activity

H2CO3 = CO2 + H2O.

Cofactor

Zinc.

Enzyme regulation

Inhibited by acetazolamide By similarity.

Subunit structure

Interacts with SLC4A4. Ref.3

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor By similarity.

Sequence similarities

Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionLyase
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbicarbonate transport

Inferred from electronic annotation. Source: Ensembl

one-carbon metabolic process

Inferred from electronic annotation. Source: InterPro

organ development

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to steroid hormone

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

anchored component of external side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

anchored component of plasma membrane

Inferred from direct assay PubMed 16083424. Source: MGI

apical plasma membrane

Inferred from electronic annotation. Source: Ensembl

brush border membrane

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: Ensembl

membrane

Inferred from direct assay PubMed 16083424. Source: MGI

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

rough endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

sarcolemma

Inferred from electronic annotation. Source: Ensembl

sarcoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

secretory granule membrane

Inferred from electronic annotation. Source: Ensembl

trans-Golgi network

Inferred from electronic annotation. Source: Ensembl

transport vesicle membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functioncarbonate dehydratase activity

Inferred from direct assay PubMed 16083424. Source: MGI

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Slc4a4O883432EBI-6859308,EBI-771342

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 277260Carbonic anhydrase 4
PRO_0000004228
Propeptide278 – 30528Removed in mature form Potential
PRO_0000004229

Regions

Region218 – 2192Substrate binding By similarity

Sites

Active site871Proton acceptor By similarity
Metal binding1141Zinc; catalytic
Metal binding1161Zinc; catalytic
Metal binding1391Zinc; catalytic

Amino acid modifications

Lipidation2771GPI-anchor amidated serine By similarity
Glycosylation1231N-linked (GlcNAc...) Potential
Glycosylation2141N-linked (GlcNAc...) Potential
Disulfide bond23 ↔ 35
Disulfide bond45 ↔ 222

Secondary structure

.................................................. 305
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q64444 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: EEE988FF52732884

FASTA30534,351
        10         20         30         40         50         60 
MQLLLALLAL AYVAPSTEDS GWCYEIQTKD PRSSCLGPEK WPGACKENQQ SPINIVTART 

        70         80         90        100        110        120 
KVNPRLTPFI LVGYDQKQQW PIKNNQHTVE MTLGGGACII GGDLPARYEA VQLHLHWSNG 

       130        140        150        160        170        180 
NDNGSEHSID GRHFAMEMHI VHKKLTSSKE DSKDKFAVLA FMIEVGDKVN KGFQPLVEAL 

       190        200        210        220        230        240 
PSISKPHSTS TVRESSLQDM LPPSTKMYTY FRYNGSLTTP NCDETVIWTV YKQPIKIHKN 

       250        260        270        280        290        300 
QFLEFSKNLY YDEDQKLNMK DNVRPLQPLG KRQVFKSHAP GQLLSLPLPT LLVPTLTCLV 


ANFLQ 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the mouse gene coding for carbonic anhydrase IV."
Tamai S., Cody L.B., Sly W.S.
Biochem. Genet. 34:31-43(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[3]"Direct extracellular interaction between carbonic anhydrase IV and the human NBC1 sodium/bicarbonate co-transporter."
Alvarez B.V., Loiselle F.B., Supuran C.T., Schwartz G.J., Casey J.R.
Biochemistry 42:12321-12329(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLC4A4.
[4]"Structures of murine carbonic anhydrase IV and human carbonic anhydrase II complexed with brinzolamide: molecular basis of isozyme-drug discrimination."
Stams T., Chen Y., Boriack-Sjodin P.A., Hurt J.D., Liao J., May J.A., Dean T., Laipis P., Silverman D.N., Christianson D.W.
Protein Sci. 7:556-563(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH INHIBITOR AND ZINC ION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37091 Genomic DNA. Translation: AAC52569.1.
BC012704 mRNA. Translation: AAH12704.1.
RefSeqNP_031633.1. NM_007607.2.
UniGeneMm.1641.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZNCX-ray2.80A22-279[»]
3ZNCX-ray2.80A22-279[»]
ProteinModelPortalQ64444.
SMRQ64444. Positions 22-279.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ64444. 2 interactions.
MINTMINT-4089751.

PTM databases

PhosphoSiteQ64444.

Proteomic databases

PaxDbQ64444.
PRIDEQ64444.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103194; ENSMUSP00000099483; ENSMUSG00000000805.
GeneID12351.
KEGGmmu:12351.
UCSCuc007krg.2. mouse.

Organism-specific databases

CTD12351.
MGIMGI:1096574. Car4.

Phylogenomic databases

eggNOGCOG3338.
GeneTreeENSGT00750000117527.
HOGENOMHOG000112637.
HOVERGENHBG002837.
InParanoidQ64444.
KOK01672.
OMAQAESSNY.
OrthoDBEOG7MD4QN.
TreeFamTF316425.

Gene expression databases

BgeeQ64444.
CleanExMM_CAR4.
GenevestigatorQ64444.

Family and domain databases

Gene3D3.10.200.10. 1 hit.
InterProIPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
IPR018343. Carbonic_anhydrase_CA4.
[Graphical view]
PANTHERPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF5. PTHR18952:SF5. 1 hit.
PfamPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMSSF51069. SSF51069. 1 hit.
PROSITEPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ64444.
NextBio281004.
PROQ64444.
SOURCESearch...

Entry information

Entry nameCAH4_MOUSE
AccessionPrimary (citable) accession number: Q64444
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot