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Protein

Sorbitol dehydrogenase

Gene

Sord

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.1 Publication

Catalytic activityi

L-iditol + NAD+ = L-sorbose + NADH.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Zinc; catalyticBy similarity
Binding sitei51 – 511SubstrateBy similarity
Metal bindingi70 – 701Zinc; catalyticBy similarity
Metal bindingi71 – 711Zinc; catalyticBy similarity
Binding sitei156 – 1561SubstrateBy similarity
Binding sitei299 – 2991SubstrateBy similarity
Binding sitei300 – 3001SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5652227. Fructose biosynthesis.
R-MMU-5661270. Catabolism of glucuronate to xylulose-5-phosphate.

Names & Taxonomyi

Protein namesi
Recommended name:
Sorbitol dehydrogenase (EC:1.1.1.14)
Alternative name(s):
L-iditol 2-dehydrogenase
Gene namesi
Name:Sord
Synonyms:Sdh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:98266. Sord.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB
  • motile cilium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Flagellum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 357356Sorbitol dehydrogenasePRO_0000000882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei169 – 1691PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ64442.
MaxQBiQ64442.
PaxDbiQ64442.
PRIDEiQ64442.

2D gel databases

REPRODUCTION-2DPAGEIPI00753038.
Q64442.

PTM databases

iPTMnetiQ64442.
PhosphoSiteiQ64442.

Expressioni

Tissue specificityi

Testis has the highest level of expression, followed by kidney, liver, and lung. Low levels of expression are also observed in lens, brain, and skeletal muscle. Expressed in sperm flagellum and very low expression in the sperm head.2 Publications

Developmental stagei

Detected early in spermatogenesis. Detected in condensing spermatids (at protein level) and is up-regulated during late spermatogenesis.1 Publication

Gene expression databases

BgeeiQ64442.
CleanExiMM_SORD.
GenevisibleiQ64442. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi203145. 1 interaction.
IntActiQ64442. 5 interactions.
MINTiMINT-1869750.
STRINGi10090.ENSMUSP00000106180.

Structurei

3D structure databases

ProteinModelPortaliQ64442.
SMRiQ64442. Positions 7-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0024. Eukaryota.
COG1063. LUCA.
GeneTreeiENSGT00550000074781.
HOGENOMiHOG000294670.
HOVERGENiHBG005484.
InParanoidiQ64442.
KOiK00008.
OMAiVTHHYDI.
OrthoDBiEOG7DRJ36.
PhylomeDBiQ64442.
TreeFamiTF313060.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPAKGENL SLVVHGPGDI RLENYPIPEL GPNDVLLKMH SVGICGSDVH
60 70 80 90 100
YWEHGRIGDF VVKKPMVLGH EAAGTVTKVG ELVKHLKPGD RVAIEPGVPR
110 120 130 140 150
EVDEYCKIGR YNLTPTIFFC ATPPDDGNLC RFYKHNADFC YKLPDSVTFE
160 170 180 190 200
EGALIEPLSV GIYACRRGSV SLGNKVLVCG AGPVGMVTLL VAKAMGAAQV
210 220 230 240 250
VVTDLSASRL TKAKEVGADF TIQVGKETPQ EIASKVESLL GSKPEVTIEC
260 270 280 290 300
TGAESSVQTG IYATHSGGTL VIVGMGAEMV NLPLVHAAIR EVDIKGVFRY
310 320 330 340 350
CNTWPMAISM LASKTLNVKP LVTHRFPLEK AVEAFETAKK GVGLKVMIKC

DPNDQNP
Length:357
Mass (Da):38,249
Last modified:July 10, 2007 - v3
Checksum:iFDAA462EF1EB6C21
GO

Sequence cautioni

The sequence AAA79043.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491V → L in AAA79043 (PubMed:7601136).Curated
Sequence conflicti259 – 2591T → S in AAA79043 (PubMed:7601136).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27014 mRNA. Translation: AAA79043.1. Different initiation.
AK004692 mRNA. Translation: BAB23478.1.
AK015059 mRNA. Translation: BAB29695.1.
AK166988 mRNA. Translation: BAE39168.1.
AK166996 mRNA. Translation: BAE39175.1.
AL844566, AL844573 Genomic DNA. Translation: CAM16325.1.
AL844573, AL844566 Genomic DNA. Translation: CAM19389.1.
BC024124 mRNA. Translation: AAH24124.1.
BC030875 mRNA. Translation: AAH30875.1.
BC092291 mRNA. Translation: AAH92291.1.
CCDSiCCDS16657.1.
PIRiS65956.
RefSeqiNP_666238.1. NM_146126.4.
UniGeneiMm.371580.
Mm.471786.

Genome annotation databases

EnsembliENSMUST00000110551; ENSMUSP00000106180; ENSMUSG00000027227.
GeneIDi20322.
KEGGimmu:20322.
UCSCiuc008maj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27014 mRNA. Translation: AAA79043.1. Different initiation.
AK004692 mRNA. Translation: BAB23478.1.
AK015059 mRNA. Translation: BAB29695.1.
AK166988 mRNA. Translation: BAE39168.1.
AK166996 mRNA. Translation: BAE39175.1.
AL844566, AL844573 Genomic DNA. Translation: CAM16325.1.
AL844573, AL844566 Genomic DNA. Translation: CAM19389.1.
BC024124 mRNA. Translation: AAH24124.1.
BC030875 mRNA. Translation: AAH30875.1.
BC092291 mRNA. Translation: AAH92291.1.
CCDSiCCDS16657.1.
PIRiS65956.
RefSeqiNP_666238.1. NM_146126.4.
UniGeneiMm.371580.
Mm.471786.

3D structure databases

ProteinModelPortaliQ64442.
SMRiQ64442. Positions 7-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203145. 1 interaction.
IntActiQ64442. 5 interactions.
MINTiMINT-1869750.
STRINGi10090.ENSMUSP00000106180.

PTM databases

iPTMnetiQ64442.
PhosphoSiteiQ64442.

2D gel databases

REPRODUCTION-2DPAGEIPI00753038.
Q64442.

Proteomic databases

EPDiQ64442.
MaxQBiQ64442.
PaxDbiQ64442.
PRIDEiQ64442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000110551; ENSMUSP00000106180; ENSMUSG00000027227.
GeneIDi20322.
KEGGimmu:20322.
UCSCiuc008maj.1. mouse.

Organism-specific databases

CTDi6652.
MGIiMGI:98266. Sord.

Phylogenomic databases

eggNOGiKOG0024. Eukaryota.
COG1063. LUCA.
GeneTreeiENSGT00550000074781.
HOGENOMiHOG000294670.
HOVERGENiHBG005484.
InParanoidiQ64442.
KOiK00008.
OMAiVTHHYDI.
OrthoDBiEOG7DRJ36.
PhylomeDBiQ64442.
TreeFamiTF313060.

Enzyme and pathway databases

ReactomeiR-MMU-5652227. Fructose biosynthesis.
R-MMU-5661270. Catabolism of glucuronate to xylulose-5-phosphate.

Miscellaneous databases

NextBioi298123.
PROiQ64442.
SOURCEiSearch...

Gene expression databases

BgeeiQ64442.
CleanExiMM_SORD.
GenevisibleiQ64442. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and determination of the sites of expression of mouse sorbitol dehydrogenase cDNA."
    Lee F.K., Lee A.Y.W., Lin C.X.F., Chung S.S.-M., Chung S.K.
    Eur. J. Biochem. 230:1059-1065(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Blastocyst, Lung and Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Liver.
  5. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Sorbitol can fuel mouse sperm motility and protein tyrosine phosphorylation via sorbitol dehydrogenase."
    Cao W., Aghajanian H.K., Haig-Ladewig L.A., Gerton G.L.
    Biol. Reprod. 80:124-133(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiDHSO_MOUSE
AccessioniPrimary (citable) accession number: Q64442
Secondary accession number(s): Q569V5, Q9CPS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 10, 2007
Last modified: May 11, 2016
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.