Q64442 (DHSO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sorbitol dehydrogenase
EC=1.1.1.14

Alternative name(s):
L-iditol 2-dehydrogenase

Gene names

Name:	Sord
Synonyms:	Sdh1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Ref.5
Catalytic activity	L-iditol + NAD+ = L-sorbose + NADH.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion membrane; Peripheral membrane protein. Cell projection › cilium › flagellum. Note: Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface. Ref.5
Tissue specificity	Testis has the highest level of expression, followed by kidney, liver, and lung. Low levels of expression are also observed in lens, brain, and skeletal muscle. Expressed in sperm flagellum and very low expression in the sperm head. Ref.1 Ref.5
Developmental stage	Detected early in spermatogenesis. Detected in condensing spermatids (at protein level) and is up-regulated during late spermatogenesis. Ref.5
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.
Sequence caution	The sequence AAA79043.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cell projection Cilium Flagellum Membrane Mitochondrion
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sperm motility Inferred from direct assay Ref.5. Source: UniProtKB
Cellular component	cilium Inferred from electronic annotation. Source: UniProtKB-KW flagellum Inferred from direct assay Ref.5. Source: UniProtKB mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from direct assay. Source: MGI
Molecular function	L-iditol 2-dehydrogenase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Sorbitol dehydrogenase		PRO_0000000882
Sites
	Metal binding	45	1	Zinc; catalytic By similarity
	Metal binding	70	1	Zinc; catalytic By similarity
	Metal binding	71	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	169	1	Phosphoserine Ref.6 Ref.7
Experimental info
	Sequence conflict	49	1	V → L in AAA79043. Ref.1
	Sequence conflict	259	1	T → S in AAA79043. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q64442 [UniParc]. Last modified July 10, 2007. Version 3. Checksum: FDAA462EF1EB6C21	FASTA	357	38,249
	10 20 30 40 50 60 MAAPAKGENL SLVVHGPGDI RLENYPIPEL GPNDVLLKMH SVGICGSDVH YWEHGRIGDF 70 80 90 100 110 120 VVKKPMVLGH EAAGTVTKVG ELVKHLKPGD RVAIEPGVPR EVDEYCKIGR YNLTPTIFFC 130 140 150 160 170 180 ATPPDDGNLC RFYKHNADFC YKLPDSVTFE EGALIEPLSV GIYACRRGSV SLGNKVLVCG 190 200 210 220 230 240 AGPVGMVTLL VAKAMGAAQV VVTDLSASRL TKAKEVGADF TIQVGKETPQ EIASKVESLL 250 260 270 280 290 300 GSKPEVTIEC TGAESSVQTG IYATHSGGTL VIVGMGAEMV NLPLVHAAIR EVDIKGVFRY 310 320 330 340 350 CNTWPMAISM LASKTLNVKP LVTHRFPLEK AVEAFETAKK GVGLKVMIKC DPNDQNP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and determination of the sites of expression of mouse sorbitol dehydrogenase cDNA." Lee F.K., Lee A.Y.W., Lin C.X.F., Chung S.S.-M., Chung S.K. Eur. J. Biochem. 230:1059-1065(1995) [PubMed: 7601136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Blastocyst, Lung and Testis.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Liver.
[5]	"Sorbitol can fuel mouse sperm motility and protein tyrosine phosphorylation via sorbitol dehydrogenase." Cao W., Aghajanian H.K., Haig-Ladewig L.A., Gerton G.L. Biol. Reprod. 80:124-133(2009) [PubMed: 18799757] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed: 18630941] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27014 mRNA. Translation: AAA79043.1. Different initiation. AK004692 mRNA. Translation: BAB23478.1. AK015059 mRNA. Translation: BAB29695.1. AK166988 mRNA. Translation: BAE39168.1. AK166996 mRNA. Translation: BAE39175.1. AL844566, AL844573 Genomic DNA. Translation: CAM16325.1. AL844573, AL844566 Genomic DNA. Translation: CAM19389.1. BC024124 mRNA. Translation: AAH24124.1. BC030875 mRNA. Translation: AAH30875.1. BC092291 mRNA. Translation: AAH92291.1.
IPI	IPI00753038.
PIR	S65956.
RefSeq	NP_666238.1. NM_146126.4.
UniGene	Mm.371580. Mm.432169.
3D structure databases
ProteinModelPortal	Q64442.
SMR	Q64442. Positions 7-357.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64442.
PTM databases
PhosphoSite	Q64442.
2D gel databases
REPRODUCTION-2DPAGE	IPI00753038. Q64442.
Proteomic databases
PRIDE	Q64442.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000110551; ENSMUSP00000106180; ENSMUSG00000027227.
GeneID	20322.
KEGG	mmu:20322.
NMPDR	fig|10090.3.peg.6788.
UCSC	uc008maj.1. mouse.
Organism-specific databases
CTD	6652.
MGI	MGI:98266. Sord.
Phylogenomic databases
eggNOG	roNOG08081.
HOGENOM	HBG753318.
HOVERGEN	HBG005484.
InParanoid	Q64442.
OrthoDB	EOG4WSW9S.
Gene expression databases
ArrayExpress	Q64442.
Bgee	Q64442.
CleanEx	MM_SORD.
Genevestigator	Q64442.
GermOnline	ENSMUSG00000027227. Mus musculus.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00008.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	298123.
SOURCE	Search...

Entry information

Entry name

DHSO_MOUSE

Accession

Primary (citable) accession number: Q64442
Secondary accession number(s): Q569V5, Q9CPS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 122 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program