1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial precursor

Contribute

Send feedback

Read comments (?) or add your own

Q64441 (CP24A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
Short name=24-OHase
Short name=Vitamin D(3) 24-hydroxylase
EC=1.14.13.126

Alternative name(s):
Cytochrome P450 24A1
Cytochrome P450-CC24

Gene names

Name:	Cyp24a1
Synonyms:	Cyp-24, Cyp24

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D₃) and calcitriol (1-alpha,25-dihydroxyvitamin D₃). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.
Catalytic activity	Calcitriol + NADPH + O₂ = calcitetrol + NADP⁺ + H₂O. Calcidiol + NADPH + O₂ = secalciferol + NADP⁺ + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion.
Induction	By 1,25-dihydroxyvitamin D₃ in kidney. Ref.3
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	osteoblast differentiation Inferred from electronic annotation. Source: Compara response to vitamin D Inferred from electronic annotation. Source: Compara vitamin D metabolic process Inferred from mutant phenotype PubMed 15225763. Source: MGI
Cellular_component	mitochondrion Inferred from direct assay PubMed 14651853 PubMed 18614015. Source: MGI
Molecular_function	1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity Inferred from electronic annotation. Source: Compara 25-hydroxycholecalciferol-24-hydroxylase activity Inferred from mutant phenotype PubMed 15225763. Source: MGI electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 35

Mitochondrion By similarity

Chain

36 – 514

479

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

PRO_0000003616

Sites

Metal binding

462

Iron (heme axial ligand) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q64441 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3D38BA9235177A42
Show »

FASTA

514

59,453

        10         20         30         40         50         60 
MSCPIDKRRP LIAFLRRLRD LGQPPRSVTS KAHVKRAPKE VPLCPLMTDG ETRNVTSLPG 

        70         80         90        100        110        120 
PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK LGSFDSVHLG SPSLLEALYR 

       130        140        150        160        170        180 
TESAHPQRLE IKPWKAYRDH RNEAYGLMIL EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN 

       190        200        210        220        230        240 
EVLADFMGQI DELRDERGRI QDLYSELNKW SFESICLVLY EKRFGLLQKD TEEEALTFIA 

       250        260        270        280        290        300 
AIKTMMSTFG KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDHRL ERYSQQPGAD 

       310        320        330        340        350        360 
FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ RLLREIQSVL 

       370        380        390        400        410        420 
PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDK PTVLGEYTLP KGTVLTLNTQ 

       430        440        450        460        470        480 
VLGSSEDNFE DADKFRPERW LEKEKKINPF AHLPFGVGKR MCIGRRLAEL QLHLALCWII 

       490        500        510 
QKYNIVATDS EPVEMLHLGI LVPSRELPIA FCPR

« Hide

References

[1]	"Molecular cloning of 25-hydroxyvitamin D-3 24-hydroxylase (Cyp-24) from mouse kidney: its inducibility by vitamin D-3." Itoh S., Yoshimura T., Iemura O., Yamada E., Tsujikawa K., Kohama Y., Mimura T. Biochim. Biophys. Acta 1264:26-28(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Kidney.
[2]	"Mouse vitamin D-24-hydroxylase: molecular cloning, tissue distribution, and transcriptional regulation by 1alpha,25-dihydroxyvitamin D3." Akeno N., Saikatsu S., Kawane T., Horiuchi N. Endocrinology 138:2233-2240(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ddY. Tissue: Kidney.
[3]	"Klotho, a gene related to a syndrome resembling human premature aging, functions in a negative regulatory circuit of vitamin D endocrine system." Tsujikawa H., Kurotaki Y., Fujimori T., Fukuda K., Nabeshima Y. Mol. Endocrinol. 17:2393-2403(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49438 mRNA. Translation: BAA08416.1. D89669 mRNA. Translation: BAA21843.1.
IPI	IPI00221624.
PIR	S60033.
RefSeq	NP_034126.1. NM_009996.3.
UniGene	Mm.6575.
3D structure databases
ProteinModelPortal	Q64441.
SMR	Q64441. Positions 51-514.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000047954.
PTM databases
PhosphoSite	Q64441.
Proteomic databases
PaxDb	Q64441.
PRIDE	Q64441.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038824; ENSMUSP00000047954; ENSMUSG00000038567.
GeneID	13081.
KEGG	mmu:13081.
UCSC	uc008ocb.1. mouse.
Organism-specific databases
CTD	1591.
MGI	MGI:88593. Cyp24a1.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00550000074304.
HOGENOM	HOG000276540.
HOVERGEN	HBG099053.
InParanoid	Q64441.
KO	K07436.
OMA	EILWKGG.
OrthoDB	EOG4PC9RZ.
Gene expression databases
ArrayExpress	Q64441.
Bgee	Q64441.
Genevestigator	Q64441.
GermOnline	ENSMUSG00000038567. Mus musculus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	283038.
SOURCE	Search...

Entry information

Entry name

CP24A_MOUSE

Accession

Primary (citable) accession number: Q64441

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents