Reviewed, UniProtKB/Swiss-Prot Q64441 (CP24A_MOUSE)

Names and origin

Protein names

Recommended name:
    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
      Short name=Vitamin D(3) 24-hydroxylase
      Short name=24-OHase
    EC=1.14.13.n4
Alternative name(s):
    Cytochrome P450 24A1
    P450-CC24

Gene names

Name:	Cyp24a1
Synonyms:	Cyp-24, Cyp24

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.
Catalytic activity	Calcitriol + NADPH + O2 = calcitetrol + NADP+ + H2O. Calcidiol + NADPH + O2 = secalciferol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion.
Induction	By 1,25-dihydroxyvitamin D3 in kidney. Ref.3
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW vitamin D metabolic process Inferred from mutant phenotype. Source: MGI
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	25-hydroxycholecalciferol-24-hydroxylase activity Inferred from mutant phenotype. Source: MGI electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion By similarity
	Chain	36 – 514	479	1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial		PRO_0000003616
Sites
	Metal binding	462	1	Iron (heme axial ligand) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q64441-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3D38BA9235177A42	FASTA	514	59,453
	10 20 30 40 50 60 MSCPIDKRRP LIAFLRRLRD LGQPPRSVTS KAHVKRAPKE VPLCPLMTDG ETRNVTSLPG 70 80 90 100 110 120 PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK LGSFDSVHLG SPSLLEALYR 130 140 150 160 170 180 TESAHPQRLE IKPWKAYRDH RNEAYGLMIL EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN 190 200 210 220 230 240 EVLADFMGQI DELRDERGRI QDLYSELNKW SFESICLVLY EKRFGLLQKD TEEEALTFIA 250 260 270 280 290 300 AIKTMMSTFG KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDHRL ERYSQQPGAD 310 320 330 340 350 360 FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ RLLREIQSVL 370 380 390 400 410 420 PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDK PTVLGEYTLP KGTVLTLNTQ 430 440 450 460 470 480 VLGSSEDNFE DADKFRPERW LEKEKKINPF AHLPFGVGKR MCIGRRLAEL QLHLALCWII 490 500 510 QKYNIVATDS EPVEMLHLGI LVPSRELPIA FCPR

References

[1]	"Molecular cloning of 25-hydroxyvitamin D-3 24-hydroxylase (Cyp-24) from mouse kidney: its inducibility by vitamin D-3." Itoh S., Yoshimura T., Iemura O., Yamada E., Tsujikawa K., Kohama Y., Mimura T. Biochim. Biophys. Acta 1264:26-28(1995) [PubMed: 7578252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Kidney.
[2]	"Mouse vitamin D-24-hydroxylase: molecular cloning, tissue distribution, and transcriptional regulation by 1alpha,25-dihydroxyvitamin D3." Akeno N., Saikatsu S., Kawane T., Horiuchi N. Endocrinology 138:2233-2240(1997) [PubMed: 9165006] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ddY. Tissue: Kidney.
[3]	"Klotho, a gene related to a syndrome resembling human premature aging, functions in a negative regulatory circuit of vitamin D endocrine system." Tsujikawa H., Kurotaki Y., Fujimori T., Fukuda K., Nabeshima Y. Mol. Endocrinol. 17:2393-2403(2003) [PubMed: 14528024] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D49438 mRNA. Translation: BAA08416.1. D89669 mRNA. Translation: BAA21843.1.
IPI	IPI00221624.
PIR	S60033.
RefSeq	NP_034126.1.
UniGene	Mm.6575
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q64441.
Genome annotation databases
Ensembl	ENSMUSG00000038567. Mus musculus. [Contig view]
GeneID	13081.
KEGG	mmu:13081.
Organism-specific databases
MGI	MGI:88593. Cyp24a1.
Phylogenomic databases
HOGENOM	Q64441.
HOVERGEN	Q64441.
OMA	Q64441. RFGLLQK.
Gene expression databases
ArrayExpress	Q64441.
Bgee	Q64441.
GermOnline	ENSMUSG00000038567. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	283038.
SOURCE	Search...

Entry information

Entry name

CP24A_MOUSE

Accession

Primary (citable) accession number: Q64441

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)