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Q64441 (CP24A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Short name=24-OHase
Short name=Vitamin D(3) 24-hydroxylase
EC=1.14.13.126
Alternative name(s):
Cytochrome P450 24A1
Cytochrome P450-CC24
Gene names
Name:Cyp24a1
Synonyms:Cyp-24, Cyp24
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.

Catalytic activity

Calcitriol + NADPH + O2 = calcitetrol + NADP+ + H2O.

Calcidiol + NADPH + O2 = secalciferol + NADP+ + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Mitochondrion.

Induction

By 1,25-dihydroxyvitamin D3 in kidney. Ref.3

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3535Mitochondrion By similarity
Chain36 – 5144791,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
PRO_0000003616

Sites

Metal binding4621Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64441 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3D38BA9235177A42

FASTA51459,453
        10         20         30         40         50         60 
MSCPIDKRRP LIAFLRRLRD LGQPPRSVTS KAHVKRAPKE VPLCPLMTDG ETRNVTSLPG 

        70         80         90        100        110        120 
PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK LGSFDSVHLG SPSLLEALYR 

       130        140        150        160        170        180 
TESAHPQRLE IKPWKAYRDH RNEAYGLMIL EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN 

       190        200        210        220        230        240 
EVLADFMGQI DELRDERGRI QDLYSELNKW SFESICLVLY EKRFGLLQKD TEEEALTFIA 

       250        260        270        280        290        300 
AIKTMMSTFG KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDHRL ERYSQQPGAD 

       310        320        330        340        350        360 
FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ RLLREIQSVL 

       370        380        390        400        410        420 
PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDK PTVLGEYTLP KGTVLTLNTQ 

       430        440        450        460        470        480 
VLGSSEDNFE DADKFRPERW LEKEKKINPF AHLPFGVGKR MCIGRRLAEL QLHLALCWII 

       490        500        510 
QKYNIVATDS EPVEMLHLGI LVPSRELPIA FCPR 

« Hide

References

[1]"Molecular cloning of 25-hydroxyvitamin D-3 24-hydroxylase (Cyp-24) from mouse kidney: its inducibility by vitamin D-3."
Itoh S., Yoshimura T., Iemura O., Yamada E., Tsujikawa K., Kohama Y., Mimura T.
Biochim. Biophys. Acta 1264:26-28(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Kidney.
[2]"Mouse vitamin D-24-hydroxylase: molecular cloning, tissue distribution, and transcriptional regulation by 1alpha,25-dihydroxyvitamin D3."
Akeno N., Saikatsu S., Kawane T., Horiuchi N.
Endocrinology 138:2233-2240(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ddY.
Tissue: Kidney.
[3]"Klotho, a gene related to a syndrome resembling human premature aging, functions in a negative regulatory circuit of vitamin D endocrine system."
Tsujikawa H., Kurotaki Y., Fujimori T., Fukuda K., Nabeshima Y.
Mol. Endocrinol. 17:2393-2403(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D49438 mRNA. Translation: BAA08416.1.
D89669 mRNA. Translation: BAA21843.1.
PIRS60033.
RefSeqNP_034126.1. NM_009996.3.
UniGeneMm.6575.

3D structure databases

ProteinModelPortalQ64441.
SMRQ64441. Positions 51-514.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000047954.

PTM databases

PhosphoSiteQ64441.

Proteomic databases

PaxDbQ64441.
PRIDEQ64441.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000038824; ENSMUSP00000047954; ENSMUSG00000038567.
GeneID13081.
KEGGmmu:13081.
UCSCuc008ocb.1. mouse.

Organism-specific databases

CTD1591.
MGIMGI:88593. Cyp24a1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117435.
HOGENOMHOG000276540.
HOVERGENHBG099053.
InParanoidQ64441.
KOK07436.
OMAQHDTLAE.
OrthoDBEOG7ZGX4B.
PhylomeDBQ64441.
TreeFamTF105094.

Gene expression databases

BgeeQ64441.
GenevestigatorQ64441.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283038.
PROQ64441.
SOURCESearch...

Entry information

Entry nameCP24A_MOUSE
AccessionPrimary (citable) accession number: Q64441
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot