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Protein

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Gene

Cyp24a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.

Catalytic activityi

Calcitriol + NADPH + O2 = calcitetrol + NADP+ + H2O.
Calcidiol + NADPH + O2 = secalciferol + NADP+ + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi462 – 4621Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity Source: MGI
  2. 25-hydroxycholecalciferol-24-hydroxylase activity Source: MGI
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro

GO - Biological processi

  1. osteoblast differentiation Source: Ensembl
  2. oxidation-reduction process Source: MGI
  3. response to vitamin D Source: MGI
  4. vitamin D metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_315248. Vitamins.
REACT_352248. Vitamin D (calciferol) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (EC:1.14.13.126)
Short name:
24-OHase
Short name:
Vitamin D(3) 24-hydroxylase
Alternative name(s):
Cytochrome P450 24A1
Cytochrome P450-CC24
Gene namesi
Name:Cyp24a1
Synonyms:Cyp-24, Cyp24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88593. Cyp24a1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535MitochondrionBy similarityAdd
BLAST
Chaini36 – 5144791,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrialPRO_0000003616Add
BLAST

Proteomic databases

PaxDbiQ64441.
PRIDEiQ64441.

PTM databases

PhosphoSiteiQ64441.

Expressioni

Inductioni

By 1,25-dihydroxyvitamin D3 in kidney.1 Publication

Gene expression databases

BgeeiQ64441.
ExpressionAtlasiQ64441. baseline.
GenevestigatoriQ64441.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047954.

Structurei

3D structure databases

ProteinModelPortaliQ64441.
SMRiQ64441. Positions 51-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000119243.
HOGENOMiHOG000276540.
HOVERGENiHBG099053.
InParanoidiQ64441.
KOiK07436.
OMAiVASPHIA.
OrthoDBiEOG7ZGX4B.
PhylomeDBiQ64441.
TreeFamiTF105094.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCPIDKRRP LIAFLRRLRD LGQPPRSVTS KAHVKRAPKE VPLCPLMTDG
60 70 80 90 100
ETRNVTSLPG PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK
110 120 130 140 150
LGSFDSVHLG SPSLLEALYR TESAHPQRLE IKPWKAYRDH RNEAYGLMIL
160 170 180 190 200
EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN EVLADFMGQI DELRDERGRI
210 220 230 240 250
QDLYSELNKW SFESICLVLY EKRFGLLQKD TEEEALTFIA AIKTMMSTFG
260 270 280 290 300
KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDHRL ERYSQQPGAD
310 320 330 340 350
FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ
360 370 380 390 400
RLLREIQSVL PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDK
410 420 430 440 450
PTVLGEYTLP KGTVLTLNTQ VLGSSEDNFE DADKFRPERW LEKEKKINPF
460 470 480 490 500
AHLPFGVGKR MCIGRRLAEL QLHLALCWII QKYNIVATDS EPVEMLHLGI
510
LVPSRELPIA FCPR
Length:514
Mass (Da):59,453
Last modified:October 31, 1996 - v1
Checksum:i3D38BA9235177A42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49438 mRNA. Translation: BAA08416.1.
D89669 mRNA. Translation: BAA21843.1.
CCDSiCCDS17122.1.
PIRiS60033.
RefSeqiNP_034126.1. NM_009996.3.
UniGeneiMm.6575.

Genome annotation databases

EnsembliENSMUST00000038824; ENSMUSP00000047954; ENSMUSG00000038567.
GeneIDi13081.
KEGGimmu:13081.
UCSCiuc008ocb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49438 mRNA. Translation: BAA08416.1.
D89669 mRNA. Translation: BAA21843.1.
CCDSiCCDS17122.1.
PIRiS60033.
RefSeqiNP_034126.1. NM_009996.3.
UniGeneiMm.6575.

3D structure databases

ProteinModelPortaliQ64441.
SMRiQ64441. Positions 51-514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047954.

PTM databases

PhosphoSiteiQ64441.

Proteomic databases

PaxDbiQ64441.
PRIDEiQ64441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038824; ENSMUSP00000047954; ENSMUSG00000038567.
GeneIDi13081.
KEGGimmu:13081.
UCSCiuc008ocb.1. mouse.

Organism-specific databases

CTDi1591.
MGIiMGI:88593. Cyp24a1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000119243.
HOGENOMiHOG000276540.
HOVERGENiHBG099053.
InParanoidiQ64441.
KOiK07436.
OMAiVASPHIA.
OrthoDBiEOG7ZGX4B.
PhylomeDBiQ64441.
TreeFamiTF105094.

Enzyme and pathway databases

ReactomeiREACT_315248. Vitamins.
REACT_352248. Vitamin D (calciferol) metabolism.

Miscellaneous databases

NextBioi283038.
PROiQ64441.
SOURCEiSearch...

Gene expression databases

BgeeiQ64441.
ExpressionAtlasiQ64441. baseline.
GenevestigatoriQ64441.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of 25-hydroxyvitamin D-3 24-hydroxylase (Cyp-24) from mouse kidney: its inducibility by vitamin D-3."
    Itoh S., Yoshimura T., Iemura O., Yamada E., Tsujikawa K., Kohama Y., Mimura T.
    Biochim. Biophys. Acta 1264:26-28(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Kidney.
  2. "Mouse vitamin D-24-hydroxylase: molecular cloning, tissue distribution, and transcriptional regulation by 1alpha,25-dihydroxyvitamin D3."
    Akeno N., Saikatsu S., Kawane T., Horiuchi N.
    Endocrinology 138:2233-2240(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
    Tissue: Kidney.
  3. "Klotho, a gene related to a syndrome resembling human premature aging, functions in a negative regulatory circuit of vitamin D endocrine system."
    Tsujikawa H., Kurotaki Y., Fujimori T., Fukuda K., Nabeshima Y.
    Mol. Endocrinol. 17:2393-2403(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCP24A_MOUSE
AccessioniPrimary (citable) accession number: Q64441
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.