Reviewed, UniProtKB/Swiss-Prot Q64436 (ATP4A_MOUSE)

Names and origin

Protein names

Recommended name:
    Potassium-transporting ATPase alpha chain 1
    EC=3.6.3.10
Alternative name(s):
    Proton pump
    Gastric H(+)/K(+) ATPase subunit alpha

Gene names

Name:

Atp4a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.
Catalytic activity	ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).
Subunit structure	Composed of two subunits: alpha (catalytic) and beta.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro pH reduction Ref.1 Inferred from direct assay. Source: MGI potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of proton transport Ref.1 Inferred from direct assay. Source: MGI response to drug Ref.1 Inferred from direct assay. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.1 Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen:potassium-exchanging ATPase activity Ref.1 Inferred from direct assay. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein heterodimerization activity Ref.1 Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1033	1032	Potassium-transporting ATPase alpha chain 1		PRO_0000046254
Regions
	Topological domain	2 – 96	95	Cytoplasmic Potential
	Transmembrane	97 – 117	21	Potential
	Topological domain	118 – 140	23	Lumenal Potential
	Transmembrane	141 – 161	21	Potential
	Topological domain	162 – 297	136	Cytoplasmic Potential
	Transmembrane	298 – 317	20	Potential
	Topological domain	318 – 329	12	Lumenal Potential
	Transmembrane	330 – 347	18	Potential
	Topological domain	348 – 781	434	Cytoplasmic Potential
	Transmembrane	782 – 801	20	Potential
	Topological domain	802 – 811	10	Lumenal Potential
	Transmembrane	812 – 832	21	Potential
	Topological domain	833 – 852	20	Cytoplasmic Potential
	Transmembrane	853 – 875	23	Potential
	Topological domain	876 – 927	52	Lumenal Potential
	Transmembrane	928 – 947	20	Potential
	Topological domain	948 – 961	14	Cytoplasmic Potential
	Transmembrane	962 – 980	19	Potential
	Topological domain	981 – 995	15	Lumenal Potential
	Transmembrane	996 – 1016	21	Potential
	Topological domain	1017 – 1033	17	Cytoplasmic Potential
Sites
	Active site	385	1	4-aspartylphosphate intermediate By similarity
	Metal binding	726	1	Magnesium By similarity
	Metal binding	730	1	Magnesium By similarity
Amino acid modifications
	Modified residue	952	1	Phosphoserine; by PKA By similarity
Experimental info
	Sequence conflict	828	1	S → F in BAB26432. Ref.3
	Sequence conflict	1030 – 1031	2	DF → EL in BAB26432. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q64436-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C2A812256439766D	FASTA	1,033	114,016
	10 20 30 40 50 60 MGKEKYELYS VELGSGPGGD MTAKMSKKKA GGGGGKKKEK LENMKKEMEI NDHQLSVSEL 70 80 90 100 110 120 EQKYQTSATK GLKASLAAEL LLRDGPNALR PPRGTPEYVK FARQLAGGLQ CLMWVAAAIC 130 140 150 160 170 180 LIAFAIQASE GDLTTDDNLY LAVALIAVVV VTGCFGYYQE FKSTNIIASF KNLVPQQATV 190 200 210 220 230 240 IRDGDKFQIN ADQLVVGDLV EMKGGDRVPA DIRILSAQGC KVDNSSLTGE SEPQTRSPEC 250 260 270 280 290 300 THESPLETRN IAFFSTMCLE GTAQGLVVST GDRTIIGRIA SLASGVENEK TPIAIEIEHF 310 320 330 340 350 360 VDIIAGLAIL FGATFFVVAM CIGYTFLRAM VFFMAIVVAY VPEGLLATVT VCLSLTAKRL 370 380 390 400 410 420 ASKNCVVKNL EAVETLGSTS VICSDKTGTL TQNRMTVSHL WFDNHIHTAD TTEDQSGQTF 430 440 450 460 470 480 DQSSETWRAL CRVLTLCNRA AFKSGQDAVP VPKRIVIGDA SETALLKFSE LTLGNAMGYR 490 500 510 520 530 540 DRFPKVCEIP FNSTNKFQLS IHTLEDPRDS RHLLVMKGAP ERVLERCSSI LIKGQELPLD 550 560 570 580 590 600 EQWREAFQTA YLSLGGLGER VLGFCQLYLN EKDYPPGYAF DVEAMNFPSS GLCFAGLVSM 610 620 630 640 650 660 IDPPRATVPD AVLKCRTAGI RVIMVTGDHP ITAKAIAASV GIISEGSETV EDIAARLRMP 670 680 690 700 710 720 VDQVNRKDAR ACVINGMQLK DMDPSELVEA LRTHPEMVFA RTSPQQKLVI VESCQRLGAI 730 740 750 760 770 780 VAVTGDGVND SPALKKADIG VAMGIAGSDA AKNAADMILL DDNFASIVTG VEQGRLIFDN 790 800 810 820 830 840 LKKSIAYTLT KNIPELTPYL IYITVSVPLP LGCITILFIE LCTDIFPSVS LAYEKAESDI 850 860 870 880 890 900 MHLRPRNPKR DRLVNEPLAA YSYFQIGAIQ SFAGFADYFT AMAQEGWFPL LCVGLRPQWE 910 920 930 940 950 960 DHHLQDLQDS YGQEWTFGQR LYQQYTCYTV FFISIEMCQI ADVLIRKTRR LSVFQQGFFR 970 980 990 1000 1010 1020 NKILVIAIVF QVCIGCFLCY CPGMPNIFNF MPIRFQWWLV PMPFGLLIFV YDEIRKLGVR 1030 CCPGSWWDQD FYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and functional expression of the mouse and frog alpha-subunit of the gastric H(+)-K(+)-ATPase." Mathews P.M., Claeys D., Jaisser F., Geering K., Horisberger J.-D., Kraehenbuhl J.-P., Rossier B.C. Am. J. Physiol. 268:C1207-C1214(1995) [PubMed: 7762614] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Gastric mucosa.
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 222-249; 369-386; 486-496; 622-634; 659-667 AND 776-783, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 822-1033. Strain: C57BL/6J. Tissue: Tongue.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U17282 mRNA. Translation: AAA79514.1. AK009676 mRNA. Translation: BAB26432.1.
IPI	IPI00230680.
PIR	I49143.
UniGene	Mm.12821
3D structure databases
HSSP	HSSP built from PDB template 1IWC based on UniProtKB P19156.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64436.
PTM databases
PhosphoSite	Q64436.
Proteomic databases
PRIDE	Q64436.
Genome annotation databases
Ensembl	ENSMUST00000005692; ENSMUSP00000005692; ENSMUSG00000005553; Mus musculus. [Genome view]
UCSC	uc009gfz.1. mouse.
Organism-specific databases
MGI	MGI:88113. Atp4a.
Phylogenomic databases
HOGENOM	Q64436.
HOVERGEN	Q64436.
Enzyme and pathway databases
BRENDA	3.6.3.10. 244.
Gene expression databases
ArrayExpress	Q64436.
Bgee	Q64436.
Genevestigator	Q64436.
GermOnline	ENSMUSG00000005553. Mus musculus.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR005775. ATPase_P-typ_cation-ex_asu_euk. IPR006069. ATPase_P-typ_cation-exchng_asu. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR015127. ATPase_P-typ_H/K-transp_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF09040. H-K_ATPase_N. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00121. NAKATPASE.
TIGRFAMs	TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

ATP4A_MOUSE

Accession

Primary (citable) accession number: Q64436
Secondary accession number(s): Q9CV46

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)