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Protein

Potassium-transporting ATPase alpha chain 1

Gene

Atp4a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3854-aspartylphosphate intermediateBy similarity1
Metal bindingi726MagnesiumBy similarity1
Metal bindingi730MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • hydrogen:potassium-exchanging ATPase activity Source: MGI
  • magnesium ion binding Source: InterPro
  • protein heterodimerization activity Source: MGI
  • sodium:potassium-exchanging ATPase activity Source: GO_Central

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: GO_Central
  • cellular potassium ion homeostasis Source: GO_Central
  • cellular sodium ion homeostasis Source: GO_Central
  • pH reduction Source: MGI
  • potassium ion import Source: GO_Central
  • regulation of proton transport Source: MGI
  • response to drug Source: MGI
  • sodium ion export from cell Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 1 (EC:3.6.3.10)
Alternative name(s):
Gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88113. Atp4a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 96CytoplasmicSequence analysisAdd BLAST95
Transmembranei97 – 117HelicalSequence analysisAdd BLAST21
Topological domaini118 – 140LumenalSequence analysisAdd BLAST23
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 297CytoplasmicSequence analysisAdd BLAST136
Transmembranei298 – 317HelicalSequence analysisAdd BLAST20
Topological domaini318 – 329LumenalSequence analysisAdd BLAST12
Transmembranei330 – 347HelicalSequence analysisAdd BLAST18
Topological domaini348 – 781CytoplasmicSequence analysisAdd BLAST434
Transmembranei782 – 801HelicalSequence analysisAdd BLAST20
Topological domaini802 – 811LumenalSequence analysis10
Transmembranei812 – 832HelicalSequence analysisAdd BLAST21
Topological domaini833 – 852CytoplasmicSequence analysisAdd BLAST20
Transmembranei853 – 875HelicalSequence analysisAdd BLAST23
Topological domaini876 – 927LumenalSequence analysisAdd BLAST52
Transmembranei928 – 947HelicalSequence analysisAdd BLAST20
Topological domaini948 – 961CytoplasmicSequence analysisAdd BLAST14
Transmembranei962 – 980HelicalSequence analysisAdd BLAST19
Topological domaini981 – 995LumenalSequence analysisAdd BLAST15
Transmembranei996 – 1016HelicalSequence analysisAdd BLAST21
Topological domaini1017 – 1033CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000462542 – 1033Potassium-transporting ATPase alpha chain 1Add BLAST1032

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphotyrosineBy similarity1
Modified residuei9PhosphotyrosineBy similarity1
Modified residuei26PhosphoserineBy similarity1
Modified residuei56PhosphoserineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei237PhosphoserineBy similarity1
Modified residuei284PhosphoserineBy similarity1
Modified residuei461PhosphoserineBy similarity1
Modified residuei599PhosphoserineBy similarity1
Modified residuei838PhosphoserineBy similarity1
Modified residuei952Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ64436.
PaxDbiQ64436.
PRIDEiQ64436.

PTM databases

iPTMnetiQ64436.
PhosphoSitePlusiQ64436.

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI

Protein-protein interaction databases

IntActiQ64436. 2 interactors.
MINTiMINT-4088799.
STRINGi10090.ENSMUSP00000131964.

Structurei

3D structure databases

ProteinModelPortaliQ64436.
SMRiQ64436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ64436.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64436-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEKYELYS VELGSGPGGD MTAKMSKKKA GGGGGKKKEK LENMKKEMEI
60 70 80 90 100
NDHQLSVSEL EQKYQTSATK GLKASLAAEL LLRDGPNALR PPRGTPEYVK
110 120 130 140 150
FARQLAGGLQ CLMWVAAAIC LIAFAIQASE GDLTTDDNLY LAVALIAVVV
160 170 180 190 200
VTGCFGYYQE FKSTNIIASF KNLVPQQATV IRDGDKFQIN ADQLVVGDLV
210 220 230 240 250
EMKGGDRVPA DIRILSAQGC KVDNSSLTGE SEPQTRSPEC THESPLETRN
260 270 280 290 300
IAFFSTMCLE GTAQGLVVST GDRTIIGRIA SLASGVENEK TPIAIEIEHF
310 320 330 340 350
VDIIAGLAIL FGATFFVVAM CIGYTFLRAM VFFMAIVVAY VPEGLLATVT
360 370 380 390 400
VCLSLTAKRL ASKNCVVKNL EAVETLGSTS VICSDKTGTL TQNRMTVSHL
410 420 430 440 450
WFDNHIHTAD TTEDQSGQTF DQSSETWRAL CRVLTLCNRA AFKSGQDAVP
460 470 480 490 500
VPKRIVIGDA SETALLKFSE LTLGNAMGYR DRFPKVCEIP FNSTNKFQLS
510 520 530 540 550
IHTLEDPRDS RHLLVMKGAP ERVLERCSSI LIKGQELPLD EQWREAFQTA
560 570 580 590 600
YLSLGGLGER VLGFCQLYLN EKDYPPGYAF DVEAMNFPSS GLCFAGLVSM
610 620 630 640 650
IDPPRATVPD AVLKCRTAGI RVIMVTGDHP ITAKAIAASV GIISEGSETV
660 670 680 690 700
EDIAARLRMP VDQVNRKDAR ACVINGMQLK DMDPSELVEA LRTHPEMVFA
710 720 730 740 750
RTSPQQKLVI VESCQRLGAI VAVTGDGVND SPALKKADIG VAMGIAGSDA
760 770 780 790 800
AKNAADMILL DDNFASIVTG VEQGRLIFDN LKKSIAYTLT KNIPELTPYL
810 820 830 840 850
IYITVSVPLP LGCITILFIE LCTDIFPSVS LAYEKAESDI MHLRPRNPKR
860 870 880 890 900
DRLVNEPLAA YSYFQIGAIQ SFAGFADYFT AMAQEGWFPL LCVGLRPQWE
910 920 930 940 950
DHHLQDLQDS YGQEWTFGQR LYQQYTCYTV FFISIEMCQI ADVLIRKTRR
960 970 980 990 1000
LSVFQQGFFR NKILVIAIVF QVCIGCFLCY CPGMPNIFNF MPIRFQWWLV
1010 1020 1030
PMPFGLLIFV YDEIRKLGVR CCPGSWWDQD FYY
Length:1,033
Mass (Da):114,016
Last modified:January 23, 2007 - v3
Checksum:iC2A812256439766D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti828S → F in BAB26432 (PubMed:16141072).Curated1
Sequence conflicti1030 – 1031DF → EL in BAB26432 (PubMed:16141072).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17282 mRNA. Translation: AAA79514.1.
AK009676 mRNA. Translation: BAB26432.1.
PIRiI49143.
UniGeneiMm.12821.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17282 mRNA. Translation: AAA79514.1.
AK009676 mRNA. Translation: BAB26432.1.
PIRiI49143.
UniGeneiMm.12821.

3D structure databases

ProteinModelPortaliQ64436.
SMRiQ64436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64436. 2 interactors.
MINTiMINT-4088799.
STRINGi10090.ENSMUSP00000131964.

PTM databases

iPTMnetiQ64436.
PhosphoSitePlusiQ64436.

Proteomic databases

MaxQBiQ64436.
PaxDbiQ64436.
PRIDEiQ64436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:88113. Atp4a.

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ64436.

Miscellaneous databases

PROiQ64436.
SOURCEiSearch...

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATP4A_MOUSE
AccessioniPrimary (citable) accession number: Q64436
Secondary accession number(s): Q9CV46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.