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Q64433 (CH10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
10 kDa heat shock protein, mitochondrial

Short name=Hsp10
Alternative name(s):
10 kDa chaperonin
Chaperonin 10
Short name=CPN10
Gene names
Name:Hspe1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length102 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. HAMAP-Rule MF_00580

Subunit structure

Homohexamer By similarity. HAMAP-Rule MF_00580

Subcellular location

Mitochondrion matrix HAMAP-Rule MF_00580.

Induction

By stress. HAMAP-Rule MF_00580

Sequence similarities

Belongs to the GroES chaperonin family.

Ontologies

Keywords
   Biological processStress response
   Cellular componentMitochondrion
   Molecular functionChaperone
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

chaperone binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 10210110 kDa heat shock protein, mitochondrial HAMAP-Rule MF_00580
PRO_0000174918

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue81N6-acetyllysine Ref.7
Modified residue281N6-succinyllysine Ref.6
Modified residue401N6-acetyllysine; alternate Ref.7
Modified residue401N6-malonyllysine; alternate By similarity
Modified residue401N6-succinyllysine; alternate Ref.6
Modified residue541N6-malonyllysine; alternate By similarity
Modified residue541N6-succinyllysine; alternate Ref.6
Modified residue561N6-acetyllysine; alternate Ref.6 Ref.7
Modified residue561N6-malonyllysine; alternate By similarity
Modified residue561N6-succinyllysine; alternate Ref.6
Modified residue661N6-acetyllysine; alternate Ref.7
Modified residue661N6-succinyllysine; alternate Ref.6
Modified residue701N6-acetyllysine; alternate Ref.7
Modified residue701N6-succinyllysine; alternate Ref.6
Modified residue791Phosphothreonine By similarity
Modified residue801N6-acetyllysine; alternate Ref.7
Modified residue801N6-succinyllysine; alternate Ref.6
Modified residue861N6-acetyllysine; alternate Ref.7
Modified residue861N6-succinyllysine; alternate Ref.6
Modified residue991N6-acetyllysine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q64433 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 08BEB5566DBE8A3D

FASTA10210,963
        10         20         30         40         50         60 
MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGGKGKSGEI 

        70         80         90        100 
EPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDSDILGKY VD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and purification of a functional nonacetylated mammalian mitochondrial chaperonin 10."
Dickson R., Larsen B., Viitanen P.V., Tormey M.B., Geske J., Strange R., Bemis L.T.
J. Biol. Chem. 269:26858-26864(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The murine chaperonin 10 gene family contains an intronless, putative gene for early pregnancy factor, Cpn10-rs1."
Fletcher B.H., Cassady A.I., Summers K.M., Cavanagh A.C.
Mamm. Genome 12:133-140(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
Tissue: Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary gland.
[5]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 8-15; 29-36; 41-54 AND 71-92, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[6]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-56, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-28; LYS-40; LYS-54; LYS-56; LYS-66; LYS-70; LYS-80 AND LYS-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast and Liver.
[7]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-8; LYS-40; LYS-56; LYS-66; LYS-70; LYS-80; LYS-86 AND LYS-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09659 mRNA. Translation: AAA62229.1.
AF251024 Genomic DNA. Translation: AAF67345.1.
AK088121 mRNA. Translation: BAC40159.1.
BC024385 mRNA. Translation: AAH24385.1.
CCDSCCDS35570.1.
PIRA55075.
RefSeqNP_032329.1. NM_008303.4.
UniGeneMm.215667.

3D structure databases

ProteinModelPortalQ64433.
SMRQ64433. Positions 11-98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ64433. 1 interaction.
MINTMINT-1839191.

PTM databases

PhosphoSiteQ64433.

2D gel databases

REPRODUCTION-2DPAGEQ64433.

Proteomic databases

MaxQBQ64433.
PaxDbQ64433.
PRIDEQ64433.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000075242; ENSMUSP00000074724; ENSMUSG00000073676.
GeneID15528.
KEGGmmu:15528.
UCSCuc007bac.2. mouse.

Organism-specific databases

CTD3336.
MGIMGI:104680. Hspe1.

Phylogenomic databases

eggNOGCOG0234.
GeneTreeENSGT00390000006350.
HOGENOMHOG000133897.
HOVERGENHBG000385.
InParanoidQ64433.
KOK04078.
OMAQDRIIVK.
OrthoDBEOG71CFPD.
PhylomeDBQ64433.
TreeFamTF313814.

Gene expression databases

ArrayExpressQ64433.
BgeeQ64433.
CleanExMM_HSPE1.
GenevestigatorQ64433.

Family and domain databases

Gene3D2.30.33.40. 1 hit.
HAMAPMF_00580. CH10.
InterProIPR020818. Chaperonin_Cpn10.
IPR018369. Chaprnonin_Cpn10_CS.
IPR011032. GroES-like.
[Graphical view]
PANTHERPTHR10772. PTHR10772. 1 hit.
PfamPF00166. Cpn10. 1 hit.
[Graphical view]
PRINTSPR00297. CHAPERONIN10.
SMARTSM00883. Cpn10. 1 hit.
[Graphical view]
SUPFAMSSF50129. SSF50129. 1 hit.
PROSITEPS00681. CHAPERONINS_CPN10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288450.
PROQ64433.
SOURCESearch...

Entry information

Entry nameCH10_MOUSE
AccessionPrimary (citable) accession number: Q64433
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot